CHANGES IN ANTIBODIES TO C1Q PREDICT RENAL RELAPSES IN SYSTEMIC LUPUS-ERYTHEMATOSUS

The presence of elevated plasma levels of autoantibodies against Clq, a subcomponent of the first component of complement in sera of patients with systemic lupus erythematosus (SLE) has been found to be associated with renal involvement, The purpose of this study was to determine whether increases in anti-Clq antibodies (anti-C1q) precede renal involvement in SLE, Forty-three SLE patients were studied longitudinally to determine the relationship between manifestations of the disease and levels of anti-Clq as well as to identify antibodies against double-stranded DNA (anti-dsDNA). Increased levels of anti-Clq were detected in all 14 of the patients who developed proliferative lupus nephritis out of 17 patients with renal relapses, which was significantly more frequent (P <0.005) than in patients with nonrenal relapses (six of 16 patients) or with inactive disease (two of 10 patients). Increased anti-dsDNA levels were observed in 14 of 17 patients with renal relapses compared with 15 of 16 patients with nonrenal relapses and five of 10 patients with inactive disease, Significant increases in anti-Clq levels prior to the relapse occurred in 10 of 14 patients who developed proliferative nephritis and in three of 16 patients with nonrenal relapses, Significant increases in anti-dsDNA levels occurred in 11 patients of the former group and in nine patients of the latter group, No significant increases in anti-Clq or anti-dsDNA levels were observed in the patients with inactive disease, The mean time period between the occurrence of a significant increase in anti-Clq or anti-dsDNA level and the moment of renal relapse for both antibodies was 2.3 months, These results suggest that an increase in anti-Clq level has a predictive value for an ensuing renal relapse of proliferative lupus nephritis, and that serial measurements of anti-Clq levels might be useful in the management of SLE patients. (C) 1995 by the National Kidney Foundation, Inc

Anti-lactoferrin antibodies and other types of anti-neutrophil cytoplasmic antibodies (ANCA) in reactive arthritis and ankylosing spondylitis

Fifty-five serum samples from patients with reactive arthritis (ReA), 40 from patients with ankylosing spondylitis (AS) and three from patients with chronic sacroiliac joint arthritis were analysed for the presence of ANCA of IgG class by means of enzyme immunosorbent assay using lactoferrin (Lf), myeloperoxidase (MPO) and antigen extracted from azurophil granules (‘α-antigen’) containing proteinase 3 (PR3) as substrate. IgG-ANCA were found in 31 (56%) patients with ReA. Twenty-three (42%) had anti-Lf antibodies, nine (16%) had anti-MPO and eight (15%) had anti-α-antigen antibodies, none of which reacted with PR3. Only six (14%) AS or sacroiliac joint arthritis patients had ANCA (P < 0.001). Three (7%) had anti-Lf, two (5%) anti-MPO and two (5%) anti-α-antigen antibodies. Yersinia and Salmonella bacteria were separated by SDS–PAGE and blots were incubated with serum from rabbits immunized with human Lf. The hyperimmune serum recognized a band of 78 kD from both bacteria which was not seen when preimmune serum was used. The reaction to the 78-kD antigen could be completely inhibited when anti-Lf antibodies were absorbed on Lf coupled to cyanogen bromide-activated Sepharose, possibly indicating cross-reacting epitopes in Lf and enterobacterial antigen