Understanding potentials and restrictions of solvent-free enzymatic polycondensation of itaconic acid: an experimental and computational analysis

6siItaconic acid is a chemically versatile unsaturated diacid that can be produced by fermentation and potentially it can replace petrol based monomers such as maleic and fumaric acids in the production of curable polyesters or new biocompatible functionalized materials. Unfortunately, due to the presence of the unsaturated C=C bond, polycondensation of itaconic acid is hampered by cross reactivity and isomerization. Therefore, enzymatic polycondensations would respond to the need of mild and selective synthetic routes for the production of novel bio-based polymers. The present work analyses the feasibility of enzymatic polycondensation of diethyl itaconate and, for the first time, provides comprehensive solutions embracing both the formulation of the biocatalyst, the reaction conditions and the choice of the co-monomers. Computational docking was used to disclose the structural factors responsible for the low reactivity of dimethyl itaconate and to identify possible solutions. Surprisingly, experimental and computational analysis revealed that 1,4-butanediol is an unsuitable co-monomer for the polycondensation of dimethyl itaconate whereas the cyclic and rigid 1,4-cyclohexanedimethanol promotes the elongation of the oligomers.partially_openembargoed_20160430Corici, Livia; Pellis, Alessandro; Ferrario, Valerio; Ebert, Cynthia; Cantone, Sara; Gardossi, LuciaCorici, Livia; Pellis, Alessandro; Ferrario, Valerio; Ebert, Cynthia; Cantone, Sara; Gardossi, Luci

Towards feasible and scalable solvent-free enzymatic polycondensations: integrating robust biocatalysts with thin film reactions

There is an enormous potential for synthesizing novel bio-based functionalized polyesters under environmentally benign conditions by exploiting the catalytic efficiency and selectivity of enzymes. Despite the wide number of studies addressing in vitro enzymatic polycondensation, insufficient progress has been documented in the last two decades towards the preparative and industrial application of this methodology. The present study analyses bottlenecks hampering the practical applicability of enzymatic polycondensation that have been most often neglected in the past, with a specific focus on solvent-free processes. Data here presented elucidate how classical approaches for enzyme immobilization combined with batch reactor configuration translate into insufficient mass transfer as well as limited recyclability of the biocatalyst. In order to overcome such bottlenecks, the present study proposes thin-film processes employing robust covalently immobilized lipases. The strategy was validated experimentally by carrying out the solvent-free polycondensation of esters of adipic and itaconic acids. The results open new perspectives for enlarging the applicability of biocatalysts in other viscous and solvent-free syntheses

Toward a fully cloudified mobile network infrastructure

Cloud computing enables the on-demand delivery of resources for a multitude of services and gives the opportunity for small agile companies to compete with large industries. In the telco world, cloud computing is currently mostly used by mobile network operators (MNO) for hosting non-critical support services and selling cloud services such as applications and data storage. MNOs are investigating the use of cloud computing to deliver key telecommunication services in the access and core networks. Without this, MNOs lose the opportunities of both combining this with over-the-top (OTT) and value-added services to their fundamental service offerings and leveraging cost-effective commodity hardware. Being able to leverage cloud computing technology effectively for the telco world is the focus of mobile cloud networking (MCN). This paper presents the key results of MCN integrated project that includes its architecture advancements, prototype implementation, and evaluation. Results show the efficiency and the simplicity that a MNO can deploy and manage the complete service lifecycle of fully cloudified, composed services that combine OTT/IT- and mobile-network-based services running on commodity hardware. The extensive performance evaluation of MCN using two key proof-of-concept scenarios that compose together many services to deliver novel converged elastic, on-demand mobile-based but innovative OTT services proves the feasibility of such fully virtualized deployments. Results show that it is beneficial to extend cloud computing to telco usage and run fully cloudified mobile-network-based systems with clear advantages and new service opportunities for MNOs and end-users

Ring opening metathesis polymerisation of a new bio-derived monomer from itaconic anhydride and furfuryl alcohol

A new oxa-norbornene bio-based lactone obtained from the 100% atom economic reaction of furfuryl alcohol and itaconic anhydride via a tandem Diels-Alder addition and lactonisation is presented. Esterification of the resulting acid gives a monomer for the production of a bio-based polymer with low polydispersity and well controlled molecular weight via ring-opening metathesis polymerisation (ROMP)

Open 5G campus networks: key drivers for 6G innovations

5G was designed to enable and unify Industrial Internet communication. Emerging 5G campus networks, in particular, provide a flexible communication infrastructure option addressing the specific needs of industry verticals regarding low latency, resilience, security, and operation models. Network Function Virtualization (NFV) and Edge Computing have paved the way for vendor-independent, customized, and scalable network designs for the past decade. Today, Open Radio Access Network (Open RAN) principles extend this architectural thinking toward an innovative and open 5G end-to-end infrastructure. 5G campus networks, in particular, might benefit from this envisaged openness. One key driver for boosting the global interest in private campus networks was the allocation of a dedicated 5G spectrum in Germany in 2019. In addition to permanent spectrum allocations for static campus network deployments, nomadic ad hoc campus network deployments using novel mechanisms, such as dynamic spectrum access and trading, also emerge. Both network types are enabled by the inherent flexibility of combining or disaggregating the desired open 5G RAN and core components in appropriate network deployments. Building upon years of experience in developing and operating 5G network cores and 5G testbeds, the authors provide an overview of the emerging global campus network market, available spectrum options, use cases for nomadic campus network deployments, and the need for open campus networks and open end-to-end technology testbeds. Utilizing the Fraunhofer FOKUS Open5GCore, the 5G Playground testbed, and the 5G+ Nomadic Node as examples, the paper sketches a blueprint for campus networks for international, applied research and development. Ending with an outlook on the evolution of campus networks, namely the transition toward higher spectrums and the integration of non-terrestrial networks, but also the adoption of more agile software principles and the deeper integration of AI/ML technologies for network control and management, it will become obvious that open campus network innovations will pave the way toward 6G

The Properties of the Transient Outward, Inward Rectifier and Acetylcholine-Sensitive Potassium Currents in Atrial Myocytes from Dogs in Sinus Rhythm and Experimentally Induced Atrial Fibrillation Dog Models

Aims: Atrial fibrillation (AF) is the most common chronic/recurrent arrhythmia, which significantly impairs quality of life and increases cardiovascular morbidity and mortality. Therefore, the aim of the present study was to investigate the properties of three repolarizing potassium currents which were shown to contribute to AF-induced electrical remodeling, i.e., the transient outward (Ito), inward rectifier (IK1) and acetylcholine-sensitive (IK,ACh) potassium currents in isolated atrial myocytes obtained from dogs either with sinus rhythm (SR) or following chronic atrial tachypacing (400/min)-induced AF. Methods: Atrial remodeling and AF were induced by chronic (4–6 weeks of) right atrial tachypacing (400/min) in dogs. Transmembrane ionic currents were measured by applying the whole-cell patch-clamp technique at 37 °C. Results: The Ito current was slightly downregulated in AF cells when compared with that recorded in SR cells. This downregulation was also associated with slowed inactivation kinetics. The IK1 current was found to be larger in AF cells; however, this upregulation was not statistically significant in the voltage range corresponding with atrial action potential (−80 mV to 0 mV). IK,ACh was activated by the cholinergic agonist carbachol (CCh; 2 µM). In SR, CCh activated a large current either in inward or outward directions. The selective IK,ACh inhibitor tertiapin (10 nM) blocked the outward CCh-induced current by 61%. In atrial cardiomyocytes isolated from dogs with AF, the presence of a constitutively active IK,ACh was observed, blocked by 59% with 10 nM tertiapin. However, in “AF atrial myocytes”, CCh activated an additional, significant ligand-dependent and tertiapin-sensitive IK,ACh current. Conclusions: In our dog AF model, Ito unlike in humans was downregulated only in a slight manner. Due to its slow inactivation kinetics, it seems that Ito may play a more significant role in atrial repolarization than in ventricular working muscle myocytes. The presence of the constitutively active IK,ACh in atrial myocytes from AF dogs shows that electrical remodeling truly developed in this model. The IK,ACh current (both ligand-dependent and constitutively active) seems to play a significant role in canine atrial electrical remodeling and may be a promising atrial selective drug target for suppressing AF

Fully renewable polyesters via polycondensation catalyzed by Thermobifida cellulosilytica cutinase 1: an integrated approach

The present study addresses comprehensively the problem of producing polyesters through sustainable processes while using fully renewable raw materials and biocatalysts. Polycondensation of bio-based dimethyl adipate with different diols was catalyzed by cutinase 1 from Thermobifida cellulosilytica (Thc_cut1) under solvent free and thin-film conditions. The biocatalyst was immobilized efficiently on a fully renewable cheap carrier based on milled rice husk. A multivariate factorial design demonstrated that Thc_cut1 is less sensitive to the presence of water in the system and it works efficiently under milder conditions (50 \ub0C; 535 mbar) when compared to lipase B from Candida antarctica (CaLB), thus enabling energy savings. Experimental and computational investigations of cutinase 1 from Thermobifida cellulosilytica (Thc_cut1) disclosed structural and functional features that make this serine-hydrolase efficient in polycondensation reactions. Bioinformatic analysis performed with the BioGPS tool pointed out functional similarities with CaLB and provided guidelines for future engineering studies aiming, for instance, at introducing different promiscuous activities in the Thc_cut1 scaffold. The results set robust premises for a full exploitation of enzymes in environmentally and economically sustainable enzymatic polycondensation reactions

Organic 6G Networks: Vision, Requirements, and Research Approaches

Building upon the significant number of already published 6G position papers, we are concentrating on the immediate next steps toward turning the research vision of software-centric networks into reality. This is accomplished, by summarizing and assessing the various requirements documents and providing a significant number of specific research directions and approaches in order to fulfill them. This article complements the existing body of work, by focusing on future core networks and their infrastructures, yet maintaining a system-level perspective and progressing in the direction of scoping key technology elements and providing high-potential research approaches for them. Additionally, we rigorously discuss the impact that different technological advancements have on the other parts of the system, to provide a coherent, end-to-end network understanding. This is in strong contrast to current approaches, where from the challenges, each research direction becomes independent and, thus, its advances are potentially cancelled out by the next technology in the chain. By maintaining this system perspective, the adoption of the different technologies becomes easier, as they are developed in unison. To address the requirements in a coherent, holistic, and unified way, we extend our high-level architecture concept named “Organic 6G Networks” towards a comprehensive end-to-end system. A holistic software-centric system, adapting the latest software development advancements from the IT industry. The Organic 6G network provides support for building a streamlined software network architecture and offers the next step on the path towards the development and specification of future mobile networks