Anti-canine Syndecan-4 as a Tool to Identify and Analyze Dystrophic Dog Satellite Cells [abstract]

Abstract only availableFaculty Mentor: Dr. Kathleen Newton, Biological SciencesMitochondria are organelles containing their own genomic DNA that are found in most eukaryotic cells. In contrast to those of animals, plant mitochondrial genomes are large and very complex in structure. While maize mitochondrial DNA can be mapped as a large single circle, it has not been observed as such.  Thus, the in vivo organization of the mitochondrial genome is not known.  Using the B37 maize inbred line, which has a 570 kilobase (kb) mitochondrial genome, observations were made regarding the possibility of sub-genomes resulting from recombination between repeats.  Mitochondria were isolated from fresh B37 seedling shoots and were placed on slides to be observed using the FISH (Fluorescent in situ Hybridization) technique.  Two probes were used, cosmid 6 in Alexafluor 488 (green) and cosmid 19 in Texas Red (red).  These cosmids are separated in the mitochondrial genome by approximately 350kb.  They also occur on separate sides of a small repeat that is known to recombine frequently.  If the genome is intact the two colors should overlap and produce a yellow color.  If there are sub-genomes, red and green should be seen individually.  The mitochondria were observed on an Olympus Spectral Imaging Microscope and the genome appeared to be fragmented most of the time. Five slides were counted and 31,077 spots were observed.  The red: green: yellow ratio was 6.44 : 2.96 :1.00.  Stretching out of an intact mitochondrial genome could cause apparent sub-genomes.  However, it is more likely that there are sub-genomes present in separate mitochondria because small mitochondria (about one micron) were selected during the isolation procedure. This is small enough that the microscope should have seen the signal as overlapping.  Future studies could extend the analysis into other maize lines and to other types of plants to determine if the sub-genomic structures are universal

A preliminary report on the contact-independent antagonism of Pseudogymnoascus destructans by Rhodococcus rhodochrous strain DAP96253.

BackgroundThe recently-identified causative agent of White-Nose Syndrome (WNS), Pseudogymnoascus destructans, has been responsible for the mortality of an estimated 5.5 million North American bats since its emergence in 2006. A primary focus of the National Response Plan, established by multiple state, federal and tribal agencies in 2011, was the identification of biological control options for WNS. In an effort to identify potential biological control options for WNS, multiply induced cells of Rhodococcus rhodochrous strain DAP96253 was screened for anti-P. destructans activity.ResultsConidia and mycelial plugs of P. destructans were exposed to induced R. rhodochrous in a closed air-space at 15°C, 7°C and 4°C and were evaluated for contact-independent inhibition of conidia germination and mycelial extension with positive results. Additionally, in situ application methods for induced R. rhodochrous, such as fixed-cell catalyst and fermentation cell-paste in non-growth conditions, were screened with positive results. R. rhodochrous was assayed for ex vivo activity via exposure to bat tissue explants inoculated with P. destructans conidia. Induced R. rhodochrous completely inhibited growth from conidia at 15°C and had a strong fungistatic effect at 4°C. Induced R. rhodochrous inhibited P. destructans growth from conidia when cultured in a shared air-space with bat tissue explants inoculated with P. destructans conidia.ConclusionThe identification of inducible biological agents with contact-independent anti- P. destructans activity is a major milestone in the development of viable biological control options for in situ application and provides the first example of contact-independent antagonism of this devastating wildlife pathogen

Recent Upgrades for the NASA Ames Vertical Gun Range

Recent upgrades to the performance capabilities of the NASA Ames Vertical Gun Range(AVGR) are presented. Upgrades include: the successful implementation of a fast-acting, gun gases suppression valve to minimize target contamination and perturbations to both the target and ejecta; powder gun and light-gas gun operational parameter adjustments to provide clean, low speed test conditions; a liquid nitrogen-based system and methodology for chilling targets and/or other impact chamber situated equipment; and imaging system capabilities enhancements to enable observing 50 micrometer particles traveling at 2 km/s. Many of these performance improvements were motivated by AVGR customer requirements for very clean shot conditions at speeds below 1.9 km/s and to provide testing in support of proposed NASA missions to Enceladus and 16-Psyche

Methane and Nitrogen Abundances On Pluto and Eris

We present spectra of Eris from the MMT 6.5 meter telescope and Red Channel Spectrograph (5700-9800 angstroms; 5 angstroms per pix) on Mt. Hopkins, AZ, and of Pluto from the Steward Observatory 2.3 meter telescope and Boller and Chivens spectrograph (7100-9400 angstroms; 2 angstroms per pix) on Kitt Peak, AZ. In addition, we present laboratory transmission spectra of methane-nitrogen and methane-argon ice mixtures. By anchoring our analysis in methane and nitrogen solubilities in one another as expressed in the phase diagram of Prokhvatilov and Yantsevich (1983), and comparing methane bands in our Eris and Pluto spectra and methane bands in our laboratory spectra of methane and nitrogen ice mixtures, we find Eris' bulk methane and nitrogen abundances are about 10% and about 90%, and Pluto's bulk methane and nitrogen abundances are about 3% and about 97%. Such abundances for Pluto are consistent with values reported in the literature. It appears that the bulk volatile composition of Eris is similar to the bulk volatile composition of Pluto. Both objects appear to be dominated by nitrogen ice. Our analysis also suggests, unlike previous work reported in the literature, that the methane and nitrogen stoichiometry is constant with depth into the surface of Eris. Finally, we point out that our Eris spectrum is also consistent with a laboratory ice mixture consisting of 40% methane and 60% argon. Although we cannot rule out an argon rich surface, it seems more likely that nitrogen is the dominant species on Eris because the nitrogen ice 2.15 micron band is seen in spectra of Pluto and Triton.Comment: The manuscript has 44 pages, 15 figures, and four tables. It will appear in the Astrophysical Journa

X-Ray photoelectron spectroscopy and mass spectrometry studies of X-ray-processed solid CO2

Solid CO2 films have been grown on a stainless steel substrate and processed by X-ray bombardment for up to 6 hr.. The reactions induced were monitored using X-ray photoelectron spectroscopy (XPS) and mass spectrometry. The XPS results are twofold: direct X-ray photolysis of the CO2 ice produced CO and an unidentified O product, possibly atomic O; secondary effects resulting from surface reactions between CO, O, and residual H from the vacuum environment produced H2CO, CH3OH, and a water ice cap on the CO2 film. The rate of production of CO from direct X-ray photolysis of CO2 is measured to be 5.4 × 102 molecule photon-1, corresponding to a formation cross section of 4.7 × 10-20 cm2. The growth rate for the water cap is calculated to be 2.6 × 10-4 monolayers s-1 for a partial pressure of H equal to 2 × 10-10 Torr. The appearance of gas-phase products from the film showed a time lag which indicates that the diffusion of the product species in the bulk CO2 is affected by some time-dependent process, possibly the creation of defects in the film. A model for the observed time dependence of the dissociation products in the gas phase yields diffusion coefficients in the CO2 of 5 × 10-12 and 1 × 10-12 cm2 s-1, for O and CO, respectively

Upgrades and Modifications of the NASA Ames HFFAF Ballistic Range

The NASA Ames Hypervelocity Free Flight Aerodynamics Facility ballistic range is described. The various configurations of the shadowgraph stations are presented. This includes the original stations with film and configurations with two different types of digital cameras. Resolution tests for the 3 shadowgraph station configurations are described. The advantages of the digital cameras are discussed, including the immediate availability of the shadowgraphs. The final shadowgraph station configuration is a mix of 26 Nikon cameras and 6 PI-MAX2 cameras. Two types of trigger light sheet stations are described visible and IR. The two gunpowders used for the NASA Ames 6.251.50 light gas guns are presented. These are the Hercules HC-33-FS powder (no longer available) and the St. Marks Powder WC 886 powder. The results from eight proof shots for the two powders are presented. Both muzzle velocities and piston velocities are 5 9 lower for the new St. Marks WC 886 powder than for the old Hercules HC-33-FS powder (no longer available). The experimental and CFD (computational) piston and muzzle velocities are in good agreement. Shadowgraph-reading software that employs template-matching pattern recognition to locate the ballistic-range model is described. Templates are generated from a 3D solid model of the ballistic-range model. The accuracy of the approach is assessed using a set of computer-generated test images

The Ames Vertical Gun Range

The Ames Vertical Gun Range (AVGR) is a national facility for conducting laboratory- scale investigations of high-speed impact processes. It provides a set of light-gas, powder, and compressed gas guns capable of accelerating projectiles to speeds up to 7 km s(exp -1). The AVGR has a unique capability to vary the angle between the projectile-launch and gravity vectors between 0 and 90 deg. The target resides in a large chamber (diameter approximately 2.5 m) that can be held at vacuum or filled with an experiment-specific atmosphere. The chamber provides a number of viewing ports and feed-throughs for data, power, and fluids. Impacts are observed via high-speed digital cameras along with investigation-specific instrumentation, such as spectrometers. Use of the range is available via grant proposals through any Planetary Science Research Program element of the NASA Research Opportunities in Space and Earth Sciences (ROSES) calls. Exploratory experiments (one to two days) are additionally possible in order to develop a new proposal

Shock Tube and Ballistic Range Facilities at NASA Ames Research Center

The Electric Arc Shock Tube (EAST) facility and the Hypervelocity Free Flight Aerodynamic Facility (HFFAF) at NASA Ames Research Center are described. These facilities have been in operation since the 1960s and have supported many NASA missions and technology development initiatives. The facilities have world-unique capabilities that enable experimental studies of real-gas aerothermal, gas dynamic, and kinetic phenomena of atmospheric entry

Questioning the Question: Review of MODERNISM AND THE HARLEM RENAISSANCE

Through my book review of Houston A. Baker, Jr.’s Modernism and the Harlem Renaissance, I examine how well Baker ingeniously transforms the question defining Harlem Renaissance scholarship from “Why did the Harlem Renaissance fail?” to “How did the Harlem Renaissance marshal black aesthetic tradition to develop a form of modernism for the New Negro?” Baker’s bold refusal to limit scholarship to the question being asked engages current readers in a previously unreachable aspect of this momentous movement

Rocket Stats

An Android application to allow Rocket League players to view their stats and track progress