Interaction of Caffeine with Model Lipid Membranes

Caffeine is not only a widely consumed active stimulant, but it is also a model molecule commonly used in pharmaceutical sciences. In this work, by performing quartz-crystal microbalance and neutron reflectometry experiments we investigate the interaction of caffeine molecules with a model lipid membrane. We determined that caffeine molecules are not able to spontaneously partition from an aqueous environment, enriched in caffeine, into a bilayer. Caffeine could be however included in solid-supported lipid bilayers if present with lipids during self-assembly. In this case, thanks to surface-sensitive techniques, we determined that caffeine molecules are preferentially located in the hydrophobic region of the membrane. These results are highly relevant for the development of new drug delivery vectors, as well as for a deeper understanding of the membrane permeation role of purine molecules

Small-angle scattering from flat bilayers containing correlated scattering length density inhomogeneities

Model lipid bilayers have been widely employed as a minimal system to investigate the structural properties of biological membranes by small-angle X-ray (SAXS) and neutron scattering (SANS) techniques. These have nanometre resolution and can give information regarding membrane thickness and scattering length densities (SLDs) of polar and apolar regions. However, biological membranes are complex systems containing different lipids and protein species, in which lipid domains can be dynamically assembled and disassembled. Therefore, SLD variations can occur within the biomembrane. In this work, a novel method has been developed to simulate SAXS and SANS profiles obtained from large unilamellar vesicles containing SLD inhomogeneities that are spatially correlated over the membrane surface. Such inhomogeneities are represented by cylindrical entities with equivalent SLDs. Stacking of bilayers is also included in the model, with no correlation between horizontal and vertical order. The model is applied to a lipid bilayer containing SLD inhomogeneities representing pores, lipid domains, and transmembrane, partially immersed and anchored proteins. It is demonstrated that all the structural information from the host lipid bilayer and from the SLD inhomogeneity can be consistently retrieved by a combined analysis of experimental SAXS and SANS data through the methodology proposed here

An Intelligent System for Electrical Energy Management in Buildings

Recent studies have highlighted that a significant part of the electrical energy consumption in residential and business buildings is due to an improper use of the electrical appliances. In this context, an automated power management system - capable of reducing energy wastes while preserving the perceived comfort level - would be extremely appealing. To this aim, we propose GreenBuilding, a sensor-based intelligent system that monitors the energy consumption and automatically controls the behavior of appliances used in a building. GreenBuilding has been implemented as a prototype and has been experimented in a real household scenario. The analysis of the experimental results highlights that GreenBuilding is able to provide significant energy savings

Rischio illuminotecnico nelle postazioni fisse di lavoro con videoterminale

Lo studio della postazione fissa di lavoro con videoterminale e l’analisi dettagliata dei requisiti ergonomici relativi agli aspetti di illuminazione e agli aspetti posturali, strettamente connessi, è divenuta oggi una attività cruciale per il soddisfacimento di condizioni di benessere, salute e sicurezza dei lavoratori. In particolare le prolungate attività di lavoro che comportano una lettura continuativa allo schermo del videoterminale richiedono una conoscenza dettagliata delle caratteristiche degli schermi. L’analisi dettagliata di postazioni di lavoro con videoterminale, scelte come casi di studio significativi, ha permesso di definire una metodologia per valutare il rischio illuminotecnico connesso con tali attività lavorative

Small-angle X-ray scattering unveils the internal structure of lipid nanoparticles

Lipid nanoparticles own a remarkable potential in nanomedicine, only partially disclosed. While the clinical use of liposomes and cationic lipid-nucleic acid complexes is well-established, liquid lipid nanoparticles (nanoemulsions), solid lipid nanoparticles, and nanostructured lipid carriers have even greater possibilities. However, they face obstacles in being used in clinics due to a lack of understanding about the molecular mechanisms controlling their drug loading and release, interactions with the biological environment (such as the protein corona), and shelf-life stability. To create effective drug delivery carriers and successfully translate bench research to clinical settings, it is crucial to have a thorough understanding of the internal structure of lipid nanoparticles. Through synchrotron small-angle X-ray scattering experiments, we determined the spatial distribution and internal structure of the nanoparticles' lipid, surfactant, and the bound water in them. The nanoparticles themselves have a barrel-like shape that consists of coplanar lipid platelets (specifically cetyl palmitate) that are covered by loosely spaced polysorbate 80 surfactant molecules, whose polar heads retain a large amount of bound water. To reduce the interface cost of bound water with unbound water without stacking, the platelets collapse onto each other. This internal structure challenges the classical core-shell model typically used to describe solid lipid nanoparticles and could play a significant role in drug loading and release, biological fluid interaction, and nanoparticle stability, making our findings valuable for the rational design of lipid-based nanoparticles

SCELTA DEFINITIVA DELLE SPECIFICHE TECNICO FUNZIONALI DI UN RADAR OTH

Rapporto finale di ricerc

Filling the 'white ribbon': a multisource seamless digital elevation model for Lizard Island, northern Great Barrier Reef

Hydrographers have traditionally referred to the nearshore area as the white ribbon' area due to the challenges associated with the collection of elevation data (elevation hereafter refers to both topography and bathymetry) in this highly dynamic transitional zone between terrestrial and marine environments. Accordingly, available information in this zone is typically characterized by a range of data sets from disparate sources. In this article, we propose a framework to fill the white ribbon area of a coral reef system by integrating multiple elevation data sets acquired by a suite of remote-sensing technologies into a seamless digital elevation model (DEM). A range of data sets are integrated, including field-collected global positioning system (GPS) elevation points, topographic and bathymetric light detecting and ranging (lidar), single and multibeam echosoundings, nautical charts, and bathymetry derived from optical remote-sensing imagery. The proposed framework ranks data reliability internally, thereby avoiding the requirements to quantify absolute error and results in a high-resolution, seamless product. Nested within this approach is an effective spatially explicit technique for improving the accuracy of bathymetry estimates derived empirically from optical satellite imagery through modelling the spatial structure of residuals. The approach was applied to data collected on and around Lizard Island in northern Australia. Collectively, the framework holds promise for filling the white ribbon zone in coastal areas characterized by similar data availability scenarios