Stiffness characteristic of front part of the vehicle

So far, in the field of forensic engineering was used to describe the stiffness of the front part of the vehicle only central stiffness characteristic that can be used with sufficient accuracy for frontal crash with a full overlap. However, if the vehicle crashes with only partial overlap so can be central stiffness characteristic limitations in the accuracy of the calculation of the deformation energy. The present article deals with the stiffness of the each parts of the front part of the vehicle for their use in forensic expert practice. Stiffness characteristics was processed for each part of the vehicle, with which you can calculate the exact deformation energy for a specific front part of the vehicle which was damaged in a frontal collision.Doposud byla v oboru soudního inženýrství využívána pro popis tuhosti přední části vozidla pouze centrální tuhostní charakteristika, kterou bylo možné použít s dostatečnou přesností pro čelní nárazy vozidla s plným překrytím. Pokud však vozidlo narazí pouze s částečným překrytím má již centrální tuhostní charakteristika svá omezení v přesnosti výpočtu deformační energie. Předmětný článek pojednává o tuhostech jednotlivých partií přední části vozidla za účelem jejich využití ve znalecké praxi. Pro každou partii předmětného vozidla je zpracována příslušná tuhostí charakteristika, s pomocí které lze vypočíst deformační energii přesně pro konkrétní část vozidla, která je při čelním nárazu poškozena

The identification of chemical compounds that decrease cellular levels of toxic Huntington's disease protein through a novel cell-based assay

Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 2006.Vita.Includes bibliographical references.Huntington's disease (HD) is a progressive degenerative neurological disorder. Individuals who inherit the IT15 gene with an expansion of the CAG repeat region inevitably succumb to increasingly sever motor, psychological, and cognitive symptoms. I sought to develop an assay system with the capability for identification of chemical compounds that selectively decrease the intracellular levels of disease-causing expanded polyglutamine huntingtin (Htt) protein without reducing the intracellular levels of the potentially protective normal Htt. To achieve this goal I designed a cell-based assay using the enzymatic activity of E. coli [beta]-galactosidase as a reporter for Htt protein levels. I expressed either expanded (97Q) or normal (23Q) Htt fused to the [beta]-galactosidase alpha-subunit ([alpha]) in an inducible fashion in PC12 cells which also expressed the [beta]-galactosidase delta-subunit ([delta]). Complementation between these expressed subunits allowed the formation of functional P-galactosidase. The level of [beta]-galactosidase activity in these [delta]-[alpha]97Q and [delta]-[alpha]23Q cells directly correlated with the amount of a 97Q and a 23Q fusion protein levels, indicating that [beta]-galactosidase activity could be used as a reporter in this system for Htt protein levels.(cont.) I implemented this cell-based assay as a secondary assay to characterize a group of compounds that had been initially identified in a High Throughput Screen because they reduced levels of expanded Htt-fragment fused to GFP. Of the 34 compounds characterized in the -galactosidase [delta]-[alpha]97Q assay, dose response curves and counter-screening with [delta]-[alpha]23Q cells revealed that seven compounds decrease [beta]-galactosidase activity only in [delta]-[alpha]97Q cells. Immunofluorescence demonstrated that two compounds decrease levels of expanded but not normal Htt proteins in the cells. Finally, tests of toxicity on HttQ103 PC12 cell lines, which show specific toxicity following expression of expanded Htt, revealed a significant correlation with the results from the [beta]-galactosidase assay and the identification of at least one compound which continued to meet the criteria for therapeutic intervention in HD. These results support the feasibility of the development of an HD therapeutic strategy based on small molecules which cause a specific reduction of intracellular expanded Htt protein levels and suggest a program of development for such molecules.by Myra Alfert Coufal.Ph.D

Orbispaces and their Mapping Spaces via Groupoids: A Categorical Approach

In this paper, we give an accessible introduction to the theory of orbispaces via groupoids. We define a certain class of topological groupoids, which we call orbigroupoids. Each orbigroupoid represents an orbispace, but just as with orbifolds and Lie groupoids, this representation is not unique: orbispaces are Morita equivalence classes of orbigroupoids. We show how to formalize this equivalence by defining the category of orbispaces as a bicatecory of fractions from the category of orbigroupoids. We focus particularly on laying the groundwork for future work in creating mapping objects for orbispaces which are themselves orbispaces, and providing a concrete description of how this mapping space construction will get its orbispace structure. Throughout this paper, we illustrate our definitions and results with numerous examples which we hope will be useful in seeing how the categorical point of view is used to study these spaces

Small heating units - emissions - fuels - choice

Bakalářská práce se ve své první části zaměřuje na přehled dostupných malých zdrojů tepelné energie. Na toto téma navazuje přehled dostupných paliv a k nim vztažené emise. Třetí částí je tvorba základního algoritmu pro volbu tepelného zdroje. V poslední části práce je navržený algoritmus aplikován na virtuálním modelovém domě Cesmína.The first part of thesis focuses on an overview of small heat energy sources. Second part is an overview of available fuels and their related emissions. The third part is the creation of the basic algorithm to select the heat source. The last part is application of the proposed model to the virtual house Cesmína.

Analysis of Power Quality Parameters in Off-Grid Systems

Import 03/11/2016Cílem této bakalářské práce je provést analýzu kvality elektrické energie v ostrovní napájecí soustavě, která není připojena k distribuční síti. Dodávka elektrické energie do této sítě pochází z obnovitelných zdrojů elektrické energie, využívající sluneční záření, s možností akumulace přebytků elektrické energie pro následné pokrytí spotřeby v době, kdy nestačí aktuální dopad úhrnu slunečního svitu na fotovoltaické panely. Předmětem této analýzy je najít vztahy nebo souvislosti mezi překročením kvalitativních parametrů elektrické energie a meteorologickými veličinami. Analyzované data pocházejí z měření v roce 2015, které započalo po změně zapojení topologie sítě Off-Grid napájecího systému z AC na DC. Naměřená data z tohoto roku mají odpovědět na klíčovou otázku z pohledu kvality elektrické energie a to, zda-li je nyní možné provozovat ostrovní napájecí systém, využívající fotovoltaické napájecí systémy, autonomně a to bez překročení stanovených velikostí limitů kvalitativních parametrů elektrické energie, zejména podle převzaté evropské normy o charakteristikách napětí elektrické energie, jež je dodávána z veřejné distribuční sítě, s označením ČSN EN 50160.The goal of this thesis is to analyze power quality in Off-Grid system, which isn't connected to distribution network. The supply of electric power to this network comes from renewable resource using solar power source with the ability to accumulate the excess of electric power to use it in times when the actual total sunlight caught on photovoltaic panels isn't enough. The aim of this analysis is to find relations between exceeding qualitative parameters and meteorological quantities. The analyzed data comes from measuring conducted in 2015 which started after the wiring change of network topology of Off-Grid feeding system from AC to DC. The data measured in that year are to answer the key question from the quality of electric energy point of view and whether it is possible to operate autonomic Off-Grid systems which uses photovoltaic supply system, without exceeding the limits of qualitative parameters of electric power, in particular these assumed from European norm labeled ČSN EN 50160 about characteristics of electric power voltage which is supplied from public distribution network.410 - Katedra elektroenergetikyvýborn

Community-acquired pneumonia in children: cell-free plasma sequencing for diagnosis and management.

Community-acquired pneumonia (CAP) is a common cause of pediatric hospital admission. Empiric antibiotic therapy for hospitalized children with serious CAP now targets the most likely pathogen(s), including those that may demonstrate significant antibiotic resistance. Cell-free plasma next-generation sequencing (CFPNGS) was first made available for Pediatric Infectious Diseases physicians in June 1, 2017, to supplement standard-of-care diagnostic techniques. A retrospective chart review was performed for children hospitalized with CAP between June 1, 2017, and January 22, 2018, to evaluate the impact of CFPNGS. We identified 15 hospitalized children with CAP without other underlying medical conditions for whom CFPNGS was performed. CFPNGS identified a pathogen in 13 of 15 (86%) children compared with 47% for those using standard culture and PCR-based methods alone. Changes in antibiotic management were made in 7 of 15 (47%) of children as a result of CFPNGS