The researcher’s erotic subjectivities : epistemological and ethical challenges

Aquest article pretén aprofundir en la qüestió de la rellevància potencial i la importància d’incloure reflexions sobre el desig i la sexualitat de la persona que investiga en els resultats de la seva recerca. Analitzem críticament l’excepcionalització de les interaccions sexual(itzade) s en la recerca: quines són les raons per les quals el contacte sexual(itzat) entre la persona que investiga i les persones participants es considera no ètic o problemàtic, i quines són les conseqüències del fet d’evitar la intimitat —o l’(auto)censura en relació amb el debat— en el treball de camp? Aquest debat ens porta a defensar una aproximació ètica alternativa a la prescrita pels protocols ètics institucionals. L’aproximació ètica que plantegem es basa en la premissa que la producció de coneixement mai no es dona fora dels nostres cossos i que la relació de recerca no és fonamentalment diferent de cap altre tipus de relació. El que proposem és una ètica relacional de la recerca que creï espais per al debat obert i en diàleg amb altres persones sobre les conseqüències (potencials) de les nostres accions com a investigadors/es/éssers humans en unes relacions d’asimetria de poder canviants.This paper aims to deepen the conversation about the potential relevance and importance of including reflection on the desire and sexuality of the researcher in research outputs. We critically scrutinise the exceptionalisation of sexual(ised) interactions in research: why is sexual(ised) contact between researchers and participants considered unethical or problematic, and what are the consequences of the avoidance of—and/or the (self-)censorship with regard to discussin —intimacy in the field? This discussion leads us to argue for an alternative ethical approach than that prescribed by institutional ethical protocols. The ethical approach that we envision is based on the premise that knowledge production never occurs apart from our bodies and that a research relationship is not fundamentally different from any other human relationship. What we propose is a relational research ethics that creates space for discussing openly and in dialogue with others the (potential) consequences of our actions as researchers/human beings within relationships of shifting power asymmetry

Development and characterization of a new human hepatic cell line

The increasing demand and hampered use of primary human hepatocytes for research purposes have urged scientists to search for alternative cell sources, such as immortalized hepatic cell lines. The aim of this study was to develop a human hepatic cell line using the combined overexpression of TERT and the cell cycle regulators cyclin D1 and mutant isoform CDK4R24C. Following transduction of adult human primary hepatocytes with the selected immortalization genes, cell growth was triggered and a cell line was established. When cultured under appropriate conditions, the cell line expressed several hepatocytic markers and liver-enriched transcription factors at the transcriptional and/or translational level, secreted liver-specific proteins and showed glycogen deposition. These results suggest that the immortalization strategy applied to primary human hepatocytes could generate a novel hepatic cell line that seems to retain some key hepatic characteristics

Architectuur en beschaving

recensie van: Wijburg, G. (2014) Architectuur en beschaving: Het smaakoffensief van de Moderne Beweging. Amsterdam: Stichting de Driehoek

Clinical procedure for colon carcinoma tissue sampling directly affects the cancer marker-capacity of VEGF family members

Background: mRNA levels of members of the Vascular Endothelial Growth Factor family (VEGF-A, -B, -C, -D, Placental Growth Factor/PlGF) have been investigated as tissue-based markers of colon cancer. These studies, which used specimens obtained by surgical resection or colonoscopic biopsy, yielded contradictory results. We studied the effect of the sampling method on the marker accuracy of VEGF family members. Methods: Comparative RT-qPCR analysis was performed on healthy colon and colon carcinoma samples obtained by biopsy (n = 38) or resection (n = 39) to measure mRNA expression levels of individual VEGF family members. mRNA levels of genes encoding the eicosanoid enzymes cyclooxygenase 2 (COX2) and 5-lipoxygenase (5-LOX) and of genes encoding the hypoxia markers glucose transporter 1 (GLUT-1) and carbonic anhydrase IX (CAIX) were included as markers for cellular stress and hypoxia. Results: Expression levels of COX2, 5-LOX, GLUT-1 and CAIX revealed the occurrence in healthy colon resection samples of hypoxic cellular stress and a concurrent increment of basal expression levels of VEGF family members. This increment abolished differential expression of VEGF-B and VEGF-C in matched carcinoma resection samples and created a surgery-induced underexpression of VEGF-D. VEGF-A and PlGF showed strong overexpression in carcinoma samples regardless of the sampling method. Conclusions: Sampling-induced hypoxia in resection samples but not in biopsy samples affects the marker-reliability of VEGF family members. Therefore, biopsy samples provide a more accurate report on VEGF family mRNA levels. Furthermore, this limited expression analysis proposes VEGF-A and PlGF as reliable, sampling procedure insensitive mRNA-markers for molecular diagnosis of colon cancer

Development and characterization of a new human hepatic cell line

The increasing demand and hampered use of primary human hepatocytes for research purposes have urged scientists to search for alternative cell sources, such as immortalized hepatic cell lines. The aim of this study was to develop a human hepatic cell line using the combined overexpression of TERT and the cell cycle regulators cyclin D1 and mutant isoform CDK4R24C. Following transduction of adult human primary hepatocytes with the selected immortalization genes, cell growth was triggered and a cell line was established. When cultured under appropriate conditions, the cell line expressed several hepatocytic markers and liver-enriched transcription factors at the transcriptional and/or translational level, secreted liver-specific proteins and showed glycogen deposition. These results suggest that the immortalization strategy applied to primary human hepatocytes could generate a novel hepatic cell line that seems to retain some key hepatic characteristics

Een kijkje in de spiegel

Dat AGORA al decennialang netwerken smeedt tussen sociaal-ruimtelijke wetenschappers, beleidsmakers en disciplines over de generaties heen is evident. Maar hoe zien deze netwerken er precies uit en wie zijn de verbindende (f)actoren hierin? Hoog tijd om de spiegel eens op te poetsen

The role of the image phase in cardiac strain imaging

International audienceThis paper reviews our most recent contributions in the field of cardiac deformation imaging, which includes a motion estimation framework based on the conservation of the image phase over time and an open pipeline to benchmark algorithms for cardiac strain imaging in 2D and 3D ultrasound. The paper also shows an original evaluation of the proposed motion estimation technique based on the new benchmarking pipeline

Fast left ventricle tracking using localized anatomical affine optical flow

Fast left ventricle tracking using localized anatomical affine optical flowIn daily clinical cardiology practice, left ventricle (LV) global and regional function assessment is crucial for disease diagnosis, therapy selection, and patient follow-up. Currently, this is still a time-consuming task, spending valuable human resources. In this work, a novel fast methodology for automatic LV tracking is proposed based on localized anatomically constrained affine optical flow. This novel method can be combined to previously proposed segmentation frameworks or manually delineated surfaces at an initial frame to obtain fully delineated datasets and, thus, assess both global and regional myocardial function. Its feasibility and accuracy were investigated in 3 distinct public databases, namely in realistically simulated 3D ultrasound, clinical 3D echocardiography, and clinical cine cardiac magnetic resonance images. The method showed accurate tracking results in all databases, proving its applicability and accuracy for myocardial function assessment. Moreover, when combined to previous state-of-the-art segmentation frameworks, it outperformed previous tracking strategies in both 3D ultrasound and cardiac magnetic resonance data, automatically computing relevant cardiac indices with smaller biases and narrower limits of agreement compared to reference indices. Simultaneously, the proposed localized tracking method showed to be suitable for online processing, even for 3D motion assessment. Importantly, although here evaluated for LV tracking only, this novel methodology is applicable for tracking of other target structures with minimal adaptations.The authors acknowledge funding support from FCT - Fundacao para a Ciência e a Tecnologia, Portugal, and the European Social Found, European Union, through the Programa Operacional Capital Humano (POCH) in the scope of the PhD grants SFRH/BD/93443/2013 (S. Queiros) and SFRH/BD/95438/2013 (P. Morais), and by the project ’PersonalizedNOS (01-0145-FEDER-000013)’ co-funded by Programa Operacional Regional do Norte (Norte2020) through the European Regional Development Fund (ERDF).info:eu-repo/semantics/publishedVersio