Introduction of 4-chloro-alpha-cyanocinnamic acid liquid matrices for high sensitivity UV-MALDI MS

Matrix-assisted laser desorption/ionization (MALDI) is a key ionization technique in mass spectrometry (MS) for the analysis of labile macromolecules. An important area of study and improvements in relation to MALDI and its application in high-sensitivity MS is that of matrix design and sample preparation. Recently, 4-chloro-alpha-cyanocinnamic acid (ClCCA) has been introduced as a new rationally designed matrix and reported to provide an improved analytical performance as demonstrated by an increase in sequence coverage of protein digests obtained by peptide mass mapping (PMM) (Jaskolla, T. W.; et al. Proc. Natl. Acad. Sci. U.S.A. 2008, 105, 12200-12205). This new matrix shows the potential to be a superior alternative to the commonly used and highly successful alpha-cyano-4-hydroxycinnamic acid (CHCA). We have taken this design one step further by developing and optimizing an ionic liquid matrix (ILM) and liquid support matrix (LSM) using ClCCA as the principle chromophore and MALDI matrix compound. These new liquid matrices possess greater sample homogeneity and a simpler morphology. The data obtained from our studies show improved sequence coverage for BSA digests compared to the traditional CHCA crystalline matrix and for the ClCCA-containing ILM a similar performance to the ClCCA crystalline matrix down to 1 fmol of BSA digest prepared in a single MALDI sample droplet with current sensitivity levels in the attomole range. The LSMs show a high tolerance to contamination such as ammonium bicarbonate, a commonly used buffering agent

Quantifying Absorption in the Transactional Interpretation

The Transactional Interpretation offers a solution to the measurement problem by identifying specific physical conditions precipitating the non-unitary `measurement transition' of von Neumann. Specifically, the transition occurs as a result of absorber response (a process lacking in the standard approach to the theory). The purpose of this Letter is to make clear that, despite recent claims to the contrary, the concepts of `absorber' and `absorber response,' as well as the process of absorption, are physically and quantitatively well-defined in the transactional picture. In addition, the Born Rule is explicitly derived for radiative processes.Comment: Final version, accepted in International Journal of Quantum Foundation

Mode-Based Sensing and Actuation Techniques for Multi-Objective Flexible Aircraft Control

Intelligent sensing and actuation designs are explored as a means to improve performance of a gust load alleviation control design for a flexible wing aircraft equipped with wing-shaping control surfaces. The proposed techniques rely on identification of the dominant structural modes during specified flight conditions and uses them as a basis for sensor placement and actuator utilization. Specifically, a strategy for sensor placement is discussed that uses target mode shape capture as a mean to improve state estimation quality. A second strategy that reduces the number of wing-shaping control inputs using mode and objective-based shape functions as virtual input channels is also presented. Both techniques are demonstrated in simulation of a flexible wing transport aircraft utilizing a multi-objective control system designed to suppress flexible motion, minimize gust and maneuver load, and reduce drag

Unsteady Crack Motion and Branching in a Phase-Field Model of Brittle Fracture

Crack propagation is studied numerically using a continuum phase-field approach to mode III brittle fracture. The results shed light on the physics that controls the speed of accelerating cracks and the characteristic branching instability at a fraction of the wave speed.Comment: 11 pages, 4 figure

New CP-violation and preferred-frame tests with polarized electrons

We used a torsion pendulum containing $\sim 9 \times 10^{22}$ polarized electrons to search for CP-violating interactions between the pendulum's electrons and unpolarized matter in the laboratory's surroundings or the sun, and to test for preferred-frame effects that would precess the electrons about a direction fixed in inertial space. We find $|g_{\rm P}^e g_{\rm S}^N|/(\hbar c)< 1.7 \times 10^{-36}$ and $|g_{\rm A}^e g_{\rm V}^N|/(\hbar c) < 4.8 \times 10^{-56}$ for $\lambda > 1$AU. Our preferred-frame constraints, interpreted in the Kosteleck\'y framework, set an upper limit on the parameter $|\bm{\tilde {b}}^e| \leq 5.0 \times 10^{-21}$ eV that should be compared to the benchmark value $m_e^2/M_{\rm Planck}= 2 \times 10^{-17}$ eV.Comment: 4 figures, accepted for publication in Physical Review Letter

Diagnostic criteria for grading the severity of acute motion sickness

Diagnostic criteria for grading severity of acute motion sicknes

On the possibility of an astronomical detection of chromaticity effects in microlensing by wormhole-like objects

We study the colour changes induced by blending in a wormhole-like microlensing scenario with extended sources. The results are compared with those obtained for limb darkening. We assess the possibility of an actual detection of the colour curve using the difference image analysis method.Comment: Accepted for publication in Modern Physics Letters A. 13 report pages, 7 figure

Parametric instabilities in magnetized multicomponent plasmas

This paper investigates the excitation of various natural modes in a magnetized bi-ion or dusty plasma. The excitation is provided by parametrically pumping the magnetic field. Here two ion-like species are allowed to be fully mobile. This generalizes our previous work where the second heavy species was taken to be stationary. Their collection of charge from the background neutral plasma modifies the dispersion properties of the pump and excited waves. The introduction of an extra mobile species adds extra modes to both these types of waves. We firstly investigate the pump wave in detail, in the case where the background magnetic field is perpendicular to the direction of propagation of the pump wave. Then we derive the dispersion equation relating the pump to the excited wave for modes propagating parallel to the background magnetic field. It is found that there are a total of twelve resonant interactions allowed, whose various growth rates are calculated and discussed.Comment: Published in May 2004; this is a late submission to the archive. 14 pages, 8 figure

Telerobotic workstation design aid

Telerobot systems are being developed to support a number of space mission applications. In low earth orbit, telerobots and teleoperated manipulators will be used in shuttle operations and space station construction/maintenance. Free flying telerobotic service vehicles will be used at low and geosynchronous orbital operations. Rovers and autonomous vehicles will be equipped with telerobotic devices in planetary exploration. In all of these systems, human operators will interact with the robot system at varied levels during the scheduled operations. The human operators may be in either orbital or ground-based control systems. To assure integrated system development and maximum utility across these systems, designers must be sensitive to the constraints and capabilities that the human brings to system operation and must be assisted in applying these human factors to system development. The simulation and analysis system is intended to serve the needs of system analysis/designers as an integrated workstation in support of telerobotic design