Identifying Spatial Relationships between Built Heritage Resources and Short-Term Rentals before the Covid-19 Pandemic: Exploratory Perspectives on Sustainability Issues

Built heritage resources (BHRs) are multidimensional assets that need to be conceived under a sustainability and circular economy framework. Whereas it is essential that their conservation, management, and enjoyment are sustainable, it is also necessary that the environmental, cultural, and socio-economic contexts in which they are integrated are sustainable too. Like other amenities, BHRs can improve the quality of the urban environment and generate externalities; additionally, they may influence sectors such as real estate, hospitality, and tourism. In this framework, this contribution aims to identify spatial relationships occurring between BHRs and short-term rentals (STRs), i.e., a recent economic phenomenon facilitated by platforms such as Airbnb. Through the application of Exploratory Spatial Data Analysis techniques and taking Turin (Italy) as a case study, this article provides evidence that spatial correlation patterns between BHRs and STRs exist, and that the areas most affected by STRs are the residential neighborhoods located in the proximity of the historic center of the city. Relations with other sets of socio-economic variables are highlighted too, and conclusions suggest that future studies are essential not only to monitor sustainability issues and reflect on new housing models and sustainable uses of buildings, but also to understand the evolution of the phenomenon in light of the pandemic Covid-19. they may influence sectors such as real estate, hospitality, and tourism. In this framework, this contribution aims to identify spatial relationships occurring between BHRs and short-term rentals (STRs), i.e., a recent economic phenomenon facilitated by platforms such as Airbnb. Through the application of Exploratory Spatial Data Analysis techniques and taking Turin (Italy) as a case study, this article provides evidence that spatial correlation patterns between BHRs and STRs exist, and that the areas most aected by STRs are the residential neighborhoods located in the proximity of the historic center of the city. Relations with other sets of socio-economic variables are highlighted too, and conclusions suggest that future studies are essential not only to monitor sustainability issues and reflect on new housing models and sustainable uses of buildings, but also to understand the evolution of the phenomenon in light of the pandemic Covid-19

Concordance in the estimation of tumor percentage in non-small cell lung cancer using digital pathology

Non-small cell lung cancer; Digital pathologyCáncer de pulmón de células no pequeñas; Patología digitalCàncer de pulmó de cèl·lules no petites; Patologia digitalThe incorporation of digital pathology in clinical practice will require the training of pathologists in digital skills. Our study aimed to assess the reliability among pathologists in determining tumor percentage in whole slide images (WSI) of non-small cell lung cancer (NSCLC) using digital image analysis, and study how the results correlate with the molecular findings. Pathologists from nine centers were trained to quantify epithelial tumor cells, tumor-associated stromal cells, and non-neoplastic cells from NSCLC WSI using QuPath. Then, we conducted two consecutive ring trials. In the first trial, analyzing four WSI, reliability between pathologists in the assessment of tumor cell percentage was poor (intraclass correlation coefficient (ICC) 0.09). After performing the first ring trial pathologists received feedback. The second trial, comprising 10 WSI with paired next-generation sequencing results, also showed poor reliability (ICC 0.24). Cases near the recommended 20% visual threshold for molecular techniques exhibited higher values with digital analysis. In the second ring trial reliability slightly improved and human errors were reduced from 5.6% to 1.25%. Most discrepancies arose from subjective tasks, such as the annotation process, suggesting potential improvement with future artificial intelligence solutions.This work has been supported by CIBERONC (grant CB16/12/00316), by Instituto de Salud Carlos III (ISCIII) through the project “PI22/01892”, by the European Union. Developed with the financial support of Immune4ALL Project (PMP22/00054), with European funds of the Recovery, Transformation and Resilence Plan; and by the Spanish Ministry of Science, Innovation and Universities under grants PID2022-141493OB-I00 and PDC2022-133865-I00 (https://doi.org/10.13039/501100011033/MCIN/AEI/ERDF, UE) - NextGenerationEU. FLR also acknowledges the support of Fundacion Mutua Madrileña (AP18051-2022), Instituto de Salud Carlos III (ISCIII) (PI22-01700, co-funded by the European Union) and the Comunidad de Madrid iLUNG Program (P2022/BMD-7437). CÓDIGO DE PROYECTO: PMP21/00107 - TÍTULO: “INtegrative GENomic, digital Imaging and clinical information towards Precision Oncology Optimization – INGENIO” - IP: LUIS PAZ-ARES RODRIGUEZ “ENTIDAD FINANCIADORA: INSTITUTO DE SALUD CARLOS III (ISCIII). PROYECTO “FINANCIADO CON CARGO A FONDOS NEXTGENERATIONEU, QUE FINANCIAN LAS ACTUACIONES DEL MRR”

Building our future, a collaborative, participatory and motivating experience for undergraduates in chemistry

One of the aspects that best captures the interest of students, and the most neglected, is the possibility of offering the practical application of what is learned, favoring experiences that make this possible. Promoting interrelation and involvement with reality is one of the most motivating elements. For all these reasons, we present the action developed in which we are committed to participatory techniques, more specifically, to the development of projects related to the professional interests of the first-year students of the Degree in Chemistry. According to the surveys we carried out with our students, the main orientations were Research, Teaching and Bio-Entrepreneurship. The specific teaching objectives were: i) to strengthen the group, integrate, disinhibit, encourage; ii) to stimulate the exchange of experiences, ideas and opinions; iii) to develop divergent and creative thinking; iv) to quickly promote student participation, capturing their interest and involving them in the teaching/learning process; and v) to promote the learning of diverse knowledge and its collective construction, among others. The experience was carried out by all the students of the Biology subject, who were distributed in work teams of 4-6 students. The groups chose a topic from among the orientations requested by the students: i) Research (Support Centres); ii) Teaching (Institute of Education Sciences); iii) Bio-Entrepreneurship (Office for the Transfer of Research Results); iv) Forensic Science Police. The dynamic of the action is based on three main phases: - Initial session. In this session, objectives, methodology and evaluation criteria were clarified. The instructors involved provided the working groups with the corresponding contact. At this point, the enquiry of the group members began. - Development sessions. Throughout several meetings with the instructors, the members of the teams had to undertake the following objectives: i) search for information from the contact provided (description of the services studied, equipment, function and applications); ii) interview with experts; iii) contextualization according to their professional interests; iv) organization of the information and preparation of communications (posters). All this was supervised by the instructors involved. They were also presented with the "digital" portfolio that they had to prepare during the course of the action. - Final session. The students presented their work in a public event at the Building our Future Conference. Assessment for learning was carried out, taking into account the influence of this one on the motivation and self-esteem of the student; and the assessment of competences, having as indicators communication skills, teamwork and responsibility, among others. The development of a "digital" portfolio per team was used: i) to improve reflection on learning, ii) to stimulate student motivation, iii) to incorporate students in the evaluation and focus it on performance, and iv) to bring the learning achieved closer to the professional tasks of the discipline. We .can conclude that the action implemented had an impact on teaching practice to the extent that what was "investigated" was motivated by the reality of professional practice, putting into context all the subjects being reviewed in the Degree and contributing to the improvement of the quality of face-to-face teaching.Universidad de Alcal

Integra BioFis 5.0. A collaborative, participatory and interdisciplinary experience for undergraduates in nursing

The pandemic has forced us to reinvent ourselves and to consider new strategies in education. Motivation, fundamental to student performance, has been seriously compromised. In this sense, the type of motivation we are interested in "fostering" is intrinsic motivation, closely linked to the concept of learning-centered goals and objectives. The action implemented is committed to the approach to challenge-based learning-gamification in the Degree in Nursing (UAH), in order to develop an integrative training with an interdisciplinary focus. Biochemistry and Physiology came together in Integra BioFis 5.0 and through participatory and collaborative techniques we pursued meaningful learning. All the students of the Biochemistry and Physiology subjects (n = 120) took part in the learning experience, organized in 12 teams. The action was carried out online through the virtual platform: - An initial session, in which the objectives, methodology, timetable and evaluation criteria were clarified. Topics that aroused the students? interest were randomly assigned (https://bit.ly/3tfJaCi). The assigned tutors guided the students in overcoming the challenges of each stage. - The development of the action consisted of a series of phases: i) documentation and literature search; ii) integration of objectives and choice of presentation format; iii) elaboration of the graphical document; iv) peer review of presentations and voting for the best contribution. The students' papers, as well as the rubrics with comments and suggestions from each of the instructors, were returned to the teams immediately. - A final session, in which they reflected on the activity they had carried out, highlighting the positive aspects of the training for the development of competences and skills: i) search for information from quality sources; ii) synthesis of contents; iii) work as a team; iv) elaboration of an original and own work. Voting was then shown for the papers presented, revealing the names of the three teams with the most votes, finalists and winners. Learning assessment was conducted by taking into account the influence of learning on motivation and the student's self-esteem and competences, with indicators such as progress, content, sources, graphical document production, teamwork and responsibility, among others. As an important element that makes gamification work, a reward for participation (awarding of a participation diploma) and for the best Integra BioFis 5.0 graphic document presented (awarding of diplomas and prizes-gifts to the members of the winning team) was considered as an important element that makes gamification work. From this educational strategy, it can be concluded that gamification is a constructive experience, taking advantage of all the benefits of implementing the overcoming of challenges in the educational environment. This has had an impact on teaching practice, to the extent that what has been "reflected" and "worked" in Integra BioFis 5.0 has contributed to improving the quality of virtual teaching, "fostering" intrinsic motivation in students.Universidad de Alcal

Induction of more aggressive tumoral phenotypes in LNCaP and PC3 cells by serum exosomes from prostate cancer patients

Prostate cancer (PCa) is the second most frequent and sixth most fatal cancer in men worldwide. Despite its high prevalence, our understanding of its etiology and the molecular mechanisms involved in the progression of the disease is substantially limited. In recent years, the potential participation of exosomes in this process has been suggested. Therefore, we aim to study the effect of exosomes isolated from the serum of patients with PCa on various cellular processes associated with increased tumor aggressiveness in two PCa cell lines: LNCaP-FGC and PC3. The exosomes were isolated by filtration wand ultracentrifugation. Their presence was confirmed by immunodetection of specific markers and their size distribution was analyzed by Dynamic Light Scattering (DLS). The results obtained demonstrated that serum exosomes from PCa patients increased migration of PC3 cells and neuroendocrine differentiation of LNCaP-FGC cells regardless of the grade of the tumor. PCa serum exosomes also enhanced the secretion of enzymes related to invasiveness and resistance to chemotherapeutics, such as extracellular matrix metalloproteases 2 and 9, and gamma-glutamyltransferase in both cell lines. Altogether, these findings support the pivotal participation of exosomes released by tumoral cells in the progression of PCa. Future studies on the molecular mechanisms involved in the observed changes could provide crucial information on this disease and help in the discovery of new therapeutic targets.Instituto de Salud Carlos III (ISCIII) P118/00526, co-funded by European Regional Development Fund (ERDF), A way to make Europe to AM

Designing a biochemical escape room for undergraduate students

Gamification is increasingly used in Higher Education to foster intrinsic motivation of undergraduate students. In this sense, we designed the Biochemical Escape Room. The students, organized in teams and through the overcoming of challenges, tackled the contents reviewed in the face-to-face classes. In addition, the tests helped in the training of different soft skills such as creative thinking, deductive thinking, collaborative work, teamwork, manual dexterity, communication and time management. The activity was designed in several phases: i) setting the learning objectives; ii) adapting the physical spaces to the teams involved in the "game"; iii) acquiring the material; iv) preparing, placing the tests and the clues and general rehearsal before D-day; and v) D-day. The results obtained after the design of the Biochemical Escape Room and its implementation show that one of the objectives of the activity had been achieved: it provided an "injection" of motivation for teachers and students. And although there are certain aspects that need to be improved, designing the escape room for the biochemistry laboratories has meant a significant change both for the instructors, in the way we teach, and for the students, in the manner they learn.Universidad de Alcalá. Vicerrectorado de Innovación Docente y Transformación Digita

Effect of Infliximab in oxidised serum albumin levels during experimental colitis.

Infliximab (IFX) is widely used in ulcerative colitis and in Crohn's disease treatment. Both diseases are characterised by increased oxidative stress, which may affect albumin oxidation. In order to test this hypothesis, the effect of IFX on colitis induced by dextran sulphate sodium (DSS) in rats was evaluated by measuring the Disease Activity Index, biochemical parameters, serum albumin oxidation and colonic mucosa oxidation. Rats with colitis showed an increase in oxidised serum albumin levels and in the oxidation of colon mucous cells. Both decreased after IFX treatment. This suggests that oxidised albumin could be a useful biomarker for monitoring inflammatory bowel disease.Universidad de AlcaláComunidad de Madri

Efecto de los exosomas de líneas celulares de próstata en la migración de células PC3. Aproximación a la funcionalización de los exosomas PC3.

10 p.El cáncer de próstata (CaP) es el segundo tipo de cáncer más frecuente en hombres y la octava causa de muerte por cáncer a nivel mundial. Actualmente, los métodos disponibles para el tratamiento son inadecuados, por carecer de especificidad, presentan elevados costes y provocan efectos secundarios graves. En los últimos años, se ha estudiado el papel de los exosomas en el cáncer debido a su función en la comunicación intercelular, promoviendo procesos como la invasión y la migración celular. En este contexto, uno de los objetivos que se plantearon fue investigar el efecto del tratamiento de la línea celular PC3 con exosomas procedentes de tres líneas celulares de próstata: i) una línea de células epiteliales humanas de próstata (RWPE-1) y ii) dos líneas tumorales que representan diferentes estadios del cáncer de próstata humano, una hormono-dependiente (LNCaP) y otra hormono-refractaria (PC3). Los exosomas fueron aislados mediante filtración y ultracentrifugación, y su presencia fue confirmada mediante inmunodetección de marcadores específicos. Los ensayos de viabilidad celular revelaron un aumento significativo en la viabilidad de las células PC3 tras 8 horas de tratamiento con los exosomas provenientes de las tres líneas celulares. Además, se observó que los exosomas derivados de PC3 incrementaron la migración celular de las células PC3. Estos hallazgos sugieren que los exosomas juegan un papel esencial en la progresión del CaP. Adicionalmente, se llevaron a cabo estudios preliminares sobre la vehiculización de compuestos con potencial terapéutico, como un conjugado de carbohidrato del 1H-imidazol[4,5- f][1,10]fenantrolina (APIP) y un metalodendrímero de rutenio, los cuales mostraron una actividad antitumoral significativa sobre las células PC3. Finalmente, se confirmó la efectividad de los procedimientos de carga de los exosomas con el metalodendrímero de Ru. No obstante, es fundamental continuar con los ensayos para validar el procedimiento de vehiculización.Máster Universitario en Dianas Terapéuticas en Señalización Celular: Investigación y Desarrollo (M132