SCUBA observations of the Horsehead Nebula - what did the horse swallow?

We present observations taken with SCUBA on the JCMT of the Horsehead Nebula in Orion (B33), at wavelengths of 450 and 850 \mum. We see bright emission from that part of the cloud associated with the photon-dominated region (PDR) at the `top' of the horse's head, which we label B33-SMM1. We characterise the physical parameters of the extended dust responsible for this emission, and find that B33-SMM1 contains a more dense core than was previously suspected. We compare the SCUBA data with data from the Infrared Space Observatory (ISO) and find that the emission at 6.75-\mum is offset towards the west, indicating that the mid-infrared emission is tracing the PDR while the submillimetre emission comes from the molecular cloud core behind the PDR. We calculate the virial balance of this core and find that it is not gravitationally bound but is being confined by the external pressure from the HII region IC434, and that it will either be destroyed by the ionising radiation, or else may undergo triggered star formation. Furthermore we find evidence for a lozenge-shaped clump in the `throat' of the horse, which is not seen in emission at shorter wavelengths. We label this source B33-SMM2 and find that it is brighter at submillimetre wavelengths than B33-SMM1. SMM2 is seen in absorption in the 6.75-\mum ISO data, from which we obtain an independent estimate of the column density in excellent agreement with that calculated from the submillimetre emission. We calculate the stability of this core against collapse and find that it is in approximate gravitational virial equilibrium. This is consistent with it being a pre-existing core in B33, possibly pre-stellar in nature, but that it may also eventually undergo collapse under the effects of the HII region.Comment: 11 pages, 6 figures, accepted by MNRA

Simulating star formation in molecular cloud cores IV. The role of turbulence and thermodynamics

We perform SPH simulations of the collapse and fragmentation of low-mass cores having different initial levels of turbulence (alpha_turb=0.05,0.10,0.25). We use a new treatment of the energy equation which captures the transport of cooling radiation against opacity due to both dust and gas (including the effects of dust sublimation, molecules, and H^- ions). We also perform comparison simulations using a standard barotropic equation of state. We find that -- when compared with the barotropic equation of state -- our more realistic treatment of the energy equation results in more protostellar objects being formed, and a higher proportion of brown dwarfs; the multiplicity frequency is essentially unchanged, but the multiple systems tend to have shorter periods (by a factor ~3), higher eccentricities, and higher mass ratios. The reason for this is that small fragments are able to cool more effectively with the new treatment, as compared with the barotropic equation of state. We find that the process of fragmentation is often bimodal. The first protostar to form is usually, at the end, the most massive, i.e. the primary. However, frequently a disc-like structure subsequently forms round this primary, and then, once it has accumulated sufficient mass, quickly fragments to produce several secondaries. We believe that this delayed fragmentation of a disc-like structure is likely to be an important source of very low-mass hydrogen-burning stars and brown dwarfs.Comment: 14 pages, 8 figures. Accepted for publication by A&

Structural analysis of the GH43 enzyme Xsa43E from Butyrivibrio proteoclasticus

The rumen of dairy cattle can be thought of as a large, stable fermentation vat and as such it houses a large and diverse community of microorganisms. The bacterium Butyrivibrio proteoclasticus is a representative of a significant component of this microbial community. It is a xylan-degrading organism whose genome encodes a large number of open reading frames annotated as fibre-degrading enzymes. This suite of enzymes is essential for the organism to utilize the plant material within the rumen as a fuel source, facilitating its survival in this competitive environment. Xsa43E, a GH43 enzyme from B. proteoclasticus, has been structurally and functionally characterized. Here, the structure of selenomethionine-derived Xsa43E determined to 1.3 Å resolution using single-wavelength anomalous diffraction is reported. Xsa43E possesses the characteristic five-bladed β-propeller domain seen in all GH43 enzymes. GH43 enzymes can have a range of functions, and the functional characterization of Xsa43E shows it to be an arabinofuranosidase capable of cleaving arabinose side chains from short segments of xylan. Full functional and structural characterization of xylan-degrading enzymes will aid in creating an enzyme cocktail that can be used to completely degrade plant material into simple sugars. These molecules have a range of applications as starting materials for many industrial processes, including renewable alternatives to fossil fuels

ATRA mechanically reprograms pancreatic stellate cells to suppress matrix remodelling and inhibit cancer cell invasion

Pancreatic ductal adenocarcinoma (PDAC) is a highly aggressive malignancy with a dismal survival rate. Persistent activation of pancreatic stellate cells (PSCs) can perturb the biomechanical homoeostasis of the tumour microenvironment to favour cancer cell invasion. Here we report that ATRA, an active metabolite of vitamin A, restores mechanical quiescence in PSCs via a mechanism involving a retinoic acid receptor beta (RAR-β)-dependent downregulation of actomyosin (MLC-2) contractility. We show that ATRA reduces the ability of PSCs to generate high traction forces and adapt to extracellular mechanical cues (mechanosensing), as well as suppresses force-mediated extracellular matrix remodelling to inhibit local cancer cell invasion in 3D organotypic models. Our findings implicate a RAR-β/MLC-2 pathway in peritumoural stromal remodelling and mechanosensory-driven activation of PSCs, and further suggest that mechanical reprogramming of PSCs with retinoic acid derivatives might be a viable alternative to stromal ablation strategies for the treatment of PDAC

The environmental stewardship program: lessons on creating long-term agri-environment schemes

The conservation of biodiversity on private land is both a high priority and a considerable challenge. An effective response to this challenge requires a combination of legislative and incentive mechanisms, coupled with preparedness by government to review and revise administrative arrangements. Preliminary results from the Environmental Stewardship Program, established by the Australian Government, highlight that there is a role for market-based approaches. However, implementation of this program through a Commonwealth bureaucracy was not without its challenges. Here we provide an overview of the program’s implementation from 2007 to 2012, followed by discussion of some key lessons learned

Direct iminization of PEEK

Semi-crystalline poly(ether ketone)s are important high-temperature engineering thermoplastics, but are difficult to characterize at the molecular level because of their insolubility in conventional organic solvents. Here we report that polymers of this type, including PEEK, react cleanly at high temperatures with low-volatility aralkyl amines to afford stable, noncrystalline poly(ether-imine)s, which are readily soluble in solvents such as chloroform, THF and DMF and so characterizable by conventional size-exclusion chromatography

Leviduction-- a study in electromagnetic levitation

Imperial Users onl

On the hierarchical classification of G Protein-Coupled Receptors

Motivation: G protein-coupled receptors (GPCRs) play an important role in many physiological systems by transducing an extracellular signal into an intracellular response. Over 50% of all marketed drugs are targeted towards a GPCR. There is considerable interest in developing an algorithm that could effectively predict the function of a GPCR from its primary sequence. Such an algorithm is useful not only in identifying novel GPCR sequences but in characterizing the interrelationships between known GPCRs. Results: An alignment-free approach to GPCR classification has been developed using techniques drawn from data mining and proteochemometrics. A dataset of over 8000 sequences was constructed to train the algorithm. This represents one of the largest GPCR datasets currently available. A predictive algorithm was developed based upon the simplest reasonable numerical representation of the protein's physicochemical properties. A selective top-down approach was developed, which used a hierarchical classifier to assign sequences to subdivisions within the GPCR hierarchy. The predictive performance of the algorithm was assessed against several standard data mining classifiers and further validated against Support Vector Machine-based GPCR prediction servers. The selective top-down approach achieves significantly higher accuracy than standard data mining methods in almost all cases

What do people do with porn? qualitative research into the consumption, use and experience of pornography and other sexually explicit media

This article reviews qualitative research into the consumption of pornography and other sexually explicit media emerging from a range of subject areas. Taking a critique of quantitative methods and a focus on measuring sexual effects and attitudes as a starting point, it considers the proposition that qualitative work is more suited to an examination of the complex social, cultural and political constructions of sexuality. Examining studies into the way men, women and young people see, experience, and use explicit media texts, the article identifies the key findings that have emerged. Qualitative work shows that sexuality explicit media texts are experienced and understood in a variety of ways and evoke strong and often contradictory reactions, not all of which are represented in public debates about pornography. These texts function in a range of different ways, depending on context; as a source of knowledge, a resource for intimate practices, a site for identity construction, and an occasion for performing gender and sexuality. The article reviews these studies and their findings, identifying what they suggest about directions for future research, both in terms of developing methodology and refining approaches to sexuality and media consumption.</p