The evaluation of the antioxidant enzyme’s activity and activity of anaerobic glycolysis’ enzymes under gradual temperature increase in Baikal amphipod species Eulimnogammаrus marituji, E. maackii and Gmelinoides fasciatus

The aim of present study was the evaluation of the antioxidant enzyme’s activity and activity of anaerobic glycolysis enzymes under gradual temperature increase in Baikal amphipods species Eulimnogammarus marituji (Baz., 1945), E. maackii (Gerstf, 1858) and Gmelinoides fasciatus (Stebb., 1899). In all species under gradual temperature increase exposition elevation of peroxidase’ activity was observed. Activities of catalase’, glutathione S – transferase’ and lactate dehydrogenase’ activity in E. marituji andE. maacki were differed from G. fasciatus. It was proposed that changes in the antioxidant enzyme’s activity and activity of anaerobic glycolysis enzymes in Baikal amphipods may contribute to increasing of glycolytic processes functional efficiency and keeping of energetic homeostasis in endemic organisms

MICROSPORIDIAN PARASITES OF SOME SPECIES ENDEMICK AMPHIPODS OF LAKE BAIKAL

With use universal microsporidian SSU rDNA primers were carry out PCR based screening of nine species endemic amphipods of lake Baikal. Six differ species microsporidian parasites were find out at two species of amphipods (P. cancel­lus and E. verrrucosus). Three parasites of uncovered microsporidian are endemick.Работа выполнена при частичной финансовой поддержке грантов ГЗ № 6.382.2014/К, РНФ 14-14-00400, Программы стратегического развития ФГБОУ ВПО «ИГУ» и совместной программы академических обменов DAAD – Минобрнауки РФ «М. Ломоносов» 2014–2015 гг

Salinity modulates thermotolerance, energy metabolism and stress response in amphipods Gammarus lacustris

Temperature and salinity are important abiotic factors for aquatic invertebrates. We investigated the influence of different salinity regimes on thermotolerance, energy metabolism and cellular stress defense mechanisms in amphipods Gammarus lacustris Sars from two populations. We exposed amphipods to different thermal scenarios and determined their survival as well as activity of major antioxidant enzymes (peroxidase, catalase, glutathione S-transferase) and parameters of energy metabolism (content of glucose, glycogen, ATP, ADP, AMP and lactate). Amphipods from a freshwater population were more sensitive to the thermal challenge, showing higher mortality during acute and gradual temperature change compared to their counterparts from a saline lake. A more thermotolerant population from a saline lake had high activity of antioxidant enzymes. The energy limitations of the freshwater population (indicated by low baseline glucose levels, downward shift of the critical temperature of aerobic metabolism and inability to maintain steady-state ATP levels during warming) was observed, possibly reflecting a trade-off between the energy demands for osmoregulation under the hypo-osmotic condition of a freshwater environment and protection against temperature stress

CHANGES IN ACTIVITY OF ANTIOXIDANT ENZYMES UNDER INCREASED SALINITY IN PALEARCTIC AMPHIPODS GAMMARUS LACUSTRIS SARS

The aim of the present study was to evaluate activity of antioxidant en­zymes (peroxidase, catalase, glutathione S-transferase) in Palearctic amphipod species Gammarus lacustris Sars under increased salinity. Activity of peroxidase and catalase was stable under combined influence of increased salinity and temperature. Opposite to this, activity of glutathione S-transferase decreased in the same conditions. Prob­ably, here we demonstrated possible participation of antioxidant enzymes in adaptation mechanisms to environmental changes in widespread amphipods G. lacustris

THE FIRST ASSESMENT OF THE ANTIOXIDANT POTENTIAL OF ACTINOBACTERIA ASSOSIATED WITH BAIKAL ENDEMIC AMPHIPODS

The study obtained the first materials confirming the hypothesis that actinobacteria associated with Baikal endemic amphipods can be oxyphils and synthesize new natural compounds with antioxidant activity.</jats:p

BIOSYNTHETIC POTENTIAL OF BAIKAL ACTINOBACTERIA CULTIVATED ON THE WOODWASHING WASTES

It has been shown that actinobacteria can grow on hard decomposable wastes and synthesize biologically active metabolites. This is a particular value for modern biopharmaceuticals and medicine in part of drug delivery and study of new natural products.</jats:p

STRAINS OF ACTINOMYCETES, ISOLATED FROM THE BAIKAL MACROINVERTEBRATES, LIKE THE PROSPECTIVE SOURCE FOR SEARCHING OF BIOACTIVE SUBSTANCES

This article provides results of the screening and an analysis of biosynthetic potential of actinomycetes, isolated from the Baikal macroinvertebrates. An analysis of mass-spec chromatograms extracts, had demonstrated that percent of identified compounds amounted to 9% (n=14), while percent of unidentified compounds amounted to 91% (n=141). Some of these identified compounds, isolated from the biomass and culture broth of the strains, had recently discovered in macroinvertebrates.Работа выполнена при частичной финансовой поддержке проектной части ГЗ № 6.382.2014/K, грантов РФФИ № 14-04-00501_а, РНФ 14-14-00400 программы стратегического развития ФГБОУ ВПО «ИГУ», а также Немецкой службы академических обменов