Effects of Simple Carbohydrate vs. Carbohydrate-Protein Intake on Glucose Homeostasis Following Intense Exercise

Kluka, J., Baskerville, J., Clifton, K., Fisher, K., Marks, D., Weidner, C., Veerabhadrappa, P. and Braun, W.A. (FACSM), Shippensburg University, Shippensburg, PA. Purpose: To test the effects of simple carbohydrate (CHO) and the combination of CHO and protein on blood glucose (BG) clearance following intense anaerobic exercise. Methods: Eight members (age = 18.8 ± 1.4 years) of the Shippensburg University Football team participated in the study. On separate test days, subjects were given either a placebo (PL) drink, a CHO drink (74 g CHO in 473 ml), or a carbohydrate-protein (C-PRO) drink (58 g CHO and 16 g PRO in 473 ml), upon completing the exercise bout. Before exercise, baseline measurements were taken for BG and heart rate. The subject then completed a 90-sec modified Wingate protocol (resistance set at 70% of the individual’s Wingate protocol resistance). BG was tested post-exercise, and every ten minutes after ingestion of the solution for 60 min. Results: Neither total revolutions completed (PL = 123.0±5.0; CHO = 126.9±3.9; C-PRO = 125.4±2.8) nor post-exercise BG (mg.dl-1) differed across treatments (PL = 88.6±6.5; CHO = 93.1±4.0; C-PRO = 101.1±4.7). However, CHO elicited the highest (p\u3c0.05) mean BG during recovery and the lowest BG was found in the PL. Conclusion: While matching post-exercise energy intake, C-PRO elicited a smaller BG area than CHO but tended to be larger than that of PL (p=0.052). C-PRO may be a better choice for post-exercise intake if aiming to maintain glucose homeostasis during recovery

Implementing telephone triage in general practice: a process evaluation of a cluster randomised controlled trial

Background: Telephone triage represents one strategy to manage demand for face-to-face GP appointments in primary care. However, limited evidence exists of the challenges GP practices face in implementing telephone triage. We conducted a qualitative process evaluation alongside a UK-based cluster randomised trial (ESTEEM) which compared the impact of GP-led and nurse-led telephone triage with usual care on primary care workload, cost, patient experience, and safety for patients requesting a same-day GP consultation. The aim of the process study was to provide insights into the observed effects of the ESTEEM trial from the perspectives of staff and patients, and to specify the circumstances under which triage is likely to be successfully implemented. Here we report perspectives of staff. Methods: The intervention comprised implementation of either GP-led or nurse-led telephone triage for a period of 2-3 months. A qualitative evaluation was conducted using staff interviews recruited from eight general practices (4 GP triage, 4 Nurse triage) in the UK, implementing triage as part of the ESTEEM trial. Qualitative interviews were undertaken with 44 staff members in GP triage and nurse triage practices (16 GPs, 8 nurses, 7 practice managers, 13 administrative staff). Results: Staff reported diverse experiences and perceptions regarding the implementation of telephone triage, its effects on workload, and on the benefits of triage. Such diversity were explained by the different ways triage was organised, the staffing models used to support triage, how the introduction of triage was communicated across practice staff, and by how staff roles were reconfigured as a result of implementing triage. Conclusion: The findings from the process evaluation offer insight into the range of ways GP practices participating in ESTEEM implemented telephone triage, and the circumstances under which telephone triage can be successfully implemented beyond the context of a clinical trial. Staff experiences and perceptions of telephone triage are shaped by the way practices communicate with staff, prepare for and sustain the changes required to implement triage effectively, as well as by existing practice culture, and staff and patient behaviour arising in response to the changes made. Trial registration: Current Controlled Trials ISRCTN20687662. Registered 28 May 2009

Symmetric Skyrmions

We present candidates for the global minimum energy solitons of charge one to nine in the Skyrme model, generated using sophisticated numerical algorithms. Assuming the Skyrme model accurately represents the low energy limit of QCD, these configurations correspond to the classical nuclear ground states of the light elements. The solitons found are particularly symmetric, for example, the charge seven skyrmion has icosahedral symmetry, and the shapes are shown to fit a remarkable sequence defined by a geometric energy minimization (GEM) rule. We also calculate the energies and sizes to within at least a few percent accuracy. These calculations provide the basis for a future investigation of the low energy vibrational modes of skyrmions and hence the possibility of testing the Skyrme model against experiment.Comment: latex, 9 pages, 1 figure (fig1.gif

Can forest management based on natural disturbances maintain ecological resilience?

Given the increasingly global stresses on forests, many ecologists argue that managers must maintain ecological resilience: the capacity of ecosystems to absorb disturbances without undergoing fundamental change. In this review we ask: Can the emerging paradigm of natural-disturbance-based management (NDBM) maintain ecological resilience in managed forests? Applying resilience theory requires careful articulation of the ecosystem state under consideration, the disturbances and stresses that affect the persistence of possible alternative states, and the spatial and temporal scales of management relevance. Implementing NDBM while maintaining resilience means recognizing that (i) biodiversity is important for long-term ecosystem persistence, (ii) natural disturbances play a critical role as a generator of structural and compositional heterogeneity at multiple scales, and (iii) traditional management tends to produce forests more homogeneous than those disturbed naturally and increases the likelihood of unexpected catastrophic change by constraining variation of key environmental processes. NDBM may maintain resilience if silvicultural strategies retain the structures and processes that perpetuate desired states while reducing those that enhance resilience of undesirable states. Such strategies require an understanding of harvesting impacts on slow ecosystem processes, such as seed-bank or nutrient dynamics, which in the long term can lead to ecological surprises by altering the forest's capacity to reorganize after disturbance

Targeted knock-down of miR21 primary transcripts using snoMEN vectors induces apoptosis in human cancer cell lines

We have previously reported an antisense technology, 'snoMEN vectors', for targeted knock-down of protein coding mRNAs using human snoRNAs manipulated to contain short regions of sequence complementarity with the mRNA target. Here we characterise the use of snoMEN vectors to target the knock-down of micro RNA primary transcripts. We document the specific knock-down of miR21 in HeLa cells using plasmid vectors expressing miR21-targeted snoMEN RNAs and show this induces apoptosis. Knock-down is dependent on the presence of complementary sequences in the snoMEN vector and the induction of apoptosis can be suppressed by over-expression of miR21. Furthermore, we have also developed lentiviral vectors for delivery of snoMEN RNAs and show this increases the efficiency of vector transduction in many human cell lines that are difficult to transfect with plasmid vectors. Transduction of lentiviral vectors expressing snoMEN targeted to pri-miR21 induces apoptosis in human lung adenocarcinoma cells, which express high levels of miR21, but not in human primary cells. We show that snoMEN-mediated suppression of miRNA expression is prevented by siRNA knock-down of Ago2, but not by knock-down of Ago1 or Upf1. snoMEN RNAs colocalise with Ago2 in cell nuclei and nucleoli and can be co-immunoprecipitated from nuclear extracts by antibodies specific for Ago2

Reconceptualizing users: The roles and activities of people as they engage with digital technologies

The concept of the user has persisted in information systems research and practice since the field’s inception in the 1950s. However, the roles that people play and the activities they perform have changed considerably over time. People now engage with digital technologies for all kinds of activities and in all kinds of ways, including for both personal and professional purposes. Hence, the purpose of this paper is to reconceptualize the term “user.” We discuss the various activities that people engage in with digital technologies, the myriad roles they play, and an essential shift in what the term “user” means. Users might be active in shaping how a particular information system is used, they might be partners with an information system, they might be used by an information system, and they may spend sizable parts of their lives living essentially within information systems. We conclude by discussing the implications for the IS discipline of reconceptualizing the term “user.

Examining the use of drama to develop epistemological understanding about the nature of science: A collective case from experience in New Zealand and England, UK

Understanding the nature of science (NoS) is perplexing for young children because it is concerned with not only understanding how evidence is generated but also what kind of meanings can be made from information collected. However, acting as a scientist-in-role, making independent decisions about what information to collect and deciding how to go about it, can enable students to experience scientific practices that empower them to better appreciate and understand the NOS. This paper illustrates how drama processes, in two international settings in Wellington, New Zealand and Oxford, United Kingdom encouraged nine to ten year old children to engage in the scientific ‘as-if’ world. The data collected from these two locations was analysed deductively to illustrate how working-in-role can influence the nature of learning and shape the scientific practices experienced that consequently inform how the NoS is understood. The children in Wellington (New Zealand) worked in-role as atmospheric scientists to design a reduced-emissions race track. The class in Oxford (UK) adopted the role of technological scientists theorising about properties of materials to create and test original carriers designed to transport a range of everyday objects. How drama promoted working-in-role to experience scientific practices supporting the understanding of the NoS, are discussed. The findings suggest that being in-role as a scientist offered learners various opportunities to be agentive, to think and act scientifically, better appreciate the nature of work that scientists do and consequently appreciate the NoS

Zero mode quantization of multi-Skyrmions

A zero mode quantization of the minimal energy SU(2) Skyrmions for nucleon numbers four to nine and seventeen is described. This involves quantizing the rotational and isorotational modes of the configurations. For nucleon numbers four, six and eight the ground states obtained are in agreement with the observed nuclear states of Helium, Lithium and Beryllium. However, for nucleon numbers five, seven, nine and seventeen the spins obtained conflict with the observed isodoublet nuclear states.Comment: 37 pages, LaTeX, 4 figures. More careful treatment of double covers, reference adde

Digital First: The Ontological Reversal and New Challenges for IS Research

The classical view of an information system is that it represents and reflects physical reality. We suggest this classical view is increasingly obsolete: digital technologies are now creating and shaping physical reality. We call this phenomenon the ontological reversal. The ontological reversal is where the digital version is created first, and the physical version second (if needed). This ontological reversal challenges us to think about the role of humans and technology in society. It also challenges us to think about our role as IS scholars in this digital world and what it means for our research agendas

A design theory for e-service environments: The interoperability challenge

The delivery of e-services across organizational boundaries poses a number of issues in terms of design of inter-organizational systems that support service delivery effectively. In this context interoperability emerges as a mandatory requirement for the design of Information Technology (IT) platforms supporting collaborative e-service environments. In this paper we address this issue by presenting a design theory for IT platforms supporting e-services based on both a deep understanding of the interoperability concept and a design research approach. Through the analysis of a cooperation framework developed in the context of an EU funded project, we instantiate the theory by providing the concrete example of a solution addressing this design problem. © 2012 Springer-Verlag GmbH Berlin Heidelberg.The delivery of e-services across organizational boundaries poses a number of issues in terms of design of inter-organizational systems that support service delivery effectively. In this context interoperability emerges as a mandatory requirement for the design of Information Technology (IT) platforms supporting collaborative e-service environments. In this paper we address this issue by presenting a design theory for IT platforms supporting e-services based on both a deep understanding of the interoperability concept and a design research approach. Through the analysis of a cooperation framework developed in the context of an EU funded project, we instantiate the theory by providing the concrete example of a solution addressing this design problem. © 2012 Springer-Verlag GmbH Berlin Heidelberg.Monograph's chapter