Poliovirus mutant that contains a cold-sensitive defect in viral RNA synthesis

By manipulating an infectious cDNA clone of poliovirus, we have introduced a single-codon insertion into the 3A region of the viral genome which has been proposed to encode a functional precursor of the virion-linked protein VPg. The resulting mutant was cold sensitive in monkey kidney cells. Viral RNA synthesis was poor at 32.5 degrees C, although no other function of the virus was obviously affected. The synthesis of both positive and negative strands was severely depressed. Temperature shift experiments suggest that a normal level of production of the affected function was required only during the early (exponential) phase of RNA synthesis. Analysis of viral polyprotein processing at the nonpermissive temperature revealed that some of the normal cleavages were not made, most likely as a consequence of the defect in RNA synthesis or as a result of the concomitant reduction in the level of virally encoded proteases

Photometric Redshift Biases from Galaxy Evolution

Proposed cosmological surveys will make use of photometric redshifts of galaxies that are significantly fainter than any complete spectroscopic redshift surveys that exist to train the photo-z methods. We investigate the photo-z biases that result from known differences between the faint and bright populations: a rise in AGN activity toward higher redshift, and a metallicity difference between intrinsically luminous and faint early-type galaxies. We find that even very small mismatches between the mean photometric target and the training set can induce photo-z biases large enough to corrupt derived cosmological parameters significantly. A metallicity shift of ~0.003dex in an old population, or contamination of any galaxy spectrum with ~0.2% AGN flux, is sufficient to induce a 10^-3 bias in photo-z. These results highlight the danger in extrapolating the behavior of bright galaxies to a fainter population, and the desirability of a spectroscopic training set that spans all of the characteristics of the photo-z targets, i.e. extending to the 25th mag or fainter galaxies that will be used in future surveys

Effects of Coulomb Coupling on Stopping Power and a Link to Macroscopic Transport

Molecular dynamics simulations are used to assess the influence of Coulomb coupling on the energy evolution of charged projectiles in the classical one-component plasma. The average projectile kinetic energy is found to decrease linearly with time when $\nu_{\alpha}/\omega_{p} \lesssim 10^{-2}$, where $\nu_{\alpha }$ is the Coulomb collision frequency between the projectile and the medium, and $\omega_{p}$ is the plasma frequency. Stopping power is obtained from the slope of this curve. In comparison to the weakly coupled limit, strong Coulomb coupling causes the magnitude of the stopping power to increase, the Bragg peak to shift to several times the plasma thermal speed, and for the stopping power curve to broaden substantially. The rate of change of the total projectile kinetic energy averaged over many independent simulations is shown to consist of two measurable components: a component associated with a one-dimensional friction force, and a thermal energy exchange rate. In the limit of a slow and massive projectile, these can be related to the macroscopic transport rates of self-diffusion and temperature relaxation in the plasma. Simulation results are compared with available theoretical models for stopping power, self-diffusion coefficients, and temperature relaxation rates.Comment: 15 pages, 11 figure

Implications and Policy Options of California's Reliance on Natural Gas

Examines existing and currently anticipated infrastructure, rising gas prices, and recurring supply problems, and looks at options to alleviate the problem. Part of a series of research reports that examines energy issues facing California

Systematic Errors in Future Weak Lensing Surveys: Requirements and Prospects for Self-Calibration

We study the impact of systematic errors on planned weak lensing surveys and compute the requirements on their contributions so that they are not a dominant source of the cosmological parameter error budget. The generic types of error we consider are multiplicative and additive errors in measurements of shear, as well as photometric redshift errors. In general, more powerful surveys have stronger systematic requirements. For example, for a SNAP-type survey the multiplicative error in shear needs to be smaller than 1%(fsky/0.025)^{-1/2} of the mean shear in any given redshift bin, while the centroids of photometric redshift bins need to be known to better than 0.003(fsky/0.025)^{-1/2}. With about a factor of two degradation in cosmological parameter errors, future surveys can enter a self-calibration regime, where the mean systematic biases are self-consistently determined from the survey and only higher-order moments of the systematics contribute. Interestingly, once the power spectrum measurements are combined with the bispectrum, the self-calibration regime in the variation of the equation of state of dark energy w_a is attained with only a 20-30% error degradation.Comment: 20 pages, 9 figures, to be submitted to MNRAS. Comments are welcom

Origin of the structural phase transition in Li7La3Zr2O12

Garnet-type Li7La3Zr2O12 (LLZO) is a solid electrolyte material with a low-conductivity tetragonal and a high-conductivity cubic phase. Using density-functional theory and variable cell shape molecular dynamics simulations, we show that the tetragonal phase stability is dependent on a simultaneous ordering of the Li ions on the Li sublattice and a volume-preserving tetragonal distortion that relieves internal structural strain. Supervalent doping introduces vacancies into the Li sublattice, increasing the overall entropy and reducing the free energy gain from ordering, eventually stabilizing the cubic phase. We show that the critical temperature for cubic phase stability is lowered as Li vacancy concentration (dopant level) is raised and that an activated hop of Li ions from one crystallographic site to another always accompanies the transition. By identifying the relevant mechanism and critical concentrations for achieving the high conductivity phase, this work shows how targeted synthesis could be used to improve electrolytic performance

Implementation of robust image artifact removal in SWarp through clipped mean stacking

We implement an algorithm for detecting and removing artifacts from astronomical images by means of outlier rejection during stacking. Our method is capable of addressing both small, highly significant artifacts such as cosmic rays and, by applying a filtering technique to generate single frame masks, larger area but lower surface brightness features such as secondary (ghost) images of bright stars. In contrast to the common method of building a median stack, the clipped or outlier-filtered mean stacked point-spread function (PSF) is a linear combination of the single frame PSFs as long as the latter are moderately homogeneous, a property of great importance for weak lensing shape measurement or model fitting photometry. In addition, it has superior noise properties, allowing a significant reduction in exposure time compared to median stacking. We make publicly available a modified version of SWarp that implements clipped mean stacking and software to generate single frame masks from the list of outlier pixels.Comment: PASP accepted; software for download at http://www.usm.uni-muenchen.de/~dgruen

Molecular collisions. 15 - Classical limit of the generalized phase shift treatment of rotational excitation - Atom-rigid rotor

Generalized phase shift approach to problem of rotationally inelastic molecular collision

Maximum Entropy/Optimal Projection (MEOP) control design synthesis: Optimal quantification of the major design tradeoffs

The underlying philosophy and motivation of the optimal projection/maximum entropy (OP/ME) stochastic modeling and reduced control design methodology for high order systems with parameter uncertainties are discussed. The OP/ME design equations for reduced-order dynamic compensation including the effect of parameter uncertainties are reviewed. The application of the methodology to several Large Space Structures (LSS) problems of representative complexity is illustrated

Slice Stretching Effects for Maximal Slicing of a Schwarzschild Black Hole

Slice stretching effects such as slice sucking and slice wrapping arise when foliating the extended Schwarzschild spacetime with maximal slices. For arbitrary spatial coordinates these effects can be quantified in the context of boundary conditions where the lapse arises as a linear combination of odd and even lapse. Favorable boundary conditions are then derived which make the overall slice stretching occur late in numerical simulations. Allowing the lapse to become negative, this requirement leads to lapse functions which approach at late times the odd lapse corresponding to the static Schwarzschild metric. Demanding in addition that a numerically favorable lapse remains non-negative, as result the average of odd and even lapse is obtained. At late times the lapse with zero gradient at the puncture arising for the puncture evolution is precisely of this form. Finally, analytic arguments are given on how slice stretching effects can be avoided. Here the excision technique and the working mechanism of the shift function are studied in detail.Comment: 16 pages, 4 figures, revised version including a study on how slice stretching can be avoided by using excision and/or shift