Understanding the causes and consequences of high-carotene Cassava roots

A large dataset (2129 data points) was developed over the years of research to increase carotenoids content in cassava roots. Correlations were always based on more than 550 data points. Correlations between dry matter content (DMC) and total carotene content (TCC) or ?- carotene (TBC) were positive and weak (0.13 and 0.07, respectively). There is no problem, therefore, for producing biofortified cassava with adequate levels of DMC. Correlation between cyanogenic potential (HCN) and TCC was negative suggesting that it is possible to obtain highcarotene with low HCN values. Correlations of data from spectrophotometer and HPLC were very high. TCC and TBC had a high correlation (0.90) suggesting that most carotenoids in cassava roots are ?-carotene. Correlations between TCC and TBC with phytoene were relatively high (0.52 and 0.61, respectively). Correlations between TCC and TBC with phytofluene were 0.60 and 0.65, respectively. Correlation between phytoene and phytofluene was 0.96. No case where accumulation of phytoene or phytofluene without parallel levels of carotenoids was observed. Similarly there was no accumulation of phytoene without parallel levels of phtytofluene. There is no evidence, therefore, that accumulation of carotenoids in cassava roots is related to a blockage at the PSY or PDS steps in the carotenoids biosynthesis. (Résumé d'auteur

Utilization of NIRS and Minolta Chromameter in selection for increased carotenoids content in cassava roots. [SP10-03]

Significant progress has been made increasing carotenoids content in cassava roots. The information was used to test the usefulness of NIRs and the Minolta Chromameter in predicting carotenoids content (and other relevant traits). Quantification was made of fresh root tissue (not lyophilized). The dataset (2129 data points) was first cleaned of outlying or suspicious data points to develop reliable prediction equations. R2 values between NIRs prediction and actual measurements were 0.91 for total carotenoids content (TCC); 0.93 for total ?-carotene (TBC), and 0.95 for dry matter content, but is less efficient for cyanogenic potential (0,81). Standard error of cross validation (SECV) for TCC and TBC were (1.191 and 0.837, respectively) while the residual predictive deviations (RPD) were also acceptable (above 3.0). These results suggest that NIRs can be used to reliably predict different variables based on fresh root samples. The Minolta Chromameter can also be used for pre-selection as its R2 values were 0.58 for TCC and 0.64 for TBC. Relative concentration of different carotenoids and precursors did not suggest the existence of a major blockage in the metabolic pathway towards the synthesis of ?-carotene. Once phytoene is synthesized most to the different pigments in the metabolic pathway are found, as expected, in correlated proportions. (Résumé d'auteur

Molecular epidemiology of domestic and sylvatic Trypanosoma cruzi infection in rural northwestern Argentina

Genetic diversity of Trypanosoma cruzi populations and parasite transmission dynamics have been well documented throughout the Americas, but few studies have been conducted in the Gran Chaco ecoregion, one of the most highly endemic areas for Chagas disease, caused by T. cruzi. In this study, we assessed the distribution of T. cruzi lineages (identified by PCR strategies) in Triatoma infestans, domestic dogs, cats, humans and sylvatic mammals from two neighbouring rural areas with different histories of transmission and vector control in northern Argentina. Lineage II predominated amongst the 99 isolates characterised and lineage I amongst the six isolates obtained from sylvatic mammals. T. cruzi lineage IIe predominated in domestic habitats; it was found in 87% of 54 isolates from Tr. infestans, in 82% of 33 isolates from dogs, and in the four cats found infected. Domestic and sylvatic cycles overlapped in the study area in the late 1980s, when intense domestic transmission occurred, and still overlap marginally. The introduction of T. cruzi from sylvatic into domestic habitats is likely to occur very rarely in the current epidemiological context. The household distribution of T. cruzi lineages showed that Tr. infestans, dogs and cats from a given house compound shared the same parasite lineage in most cases. Based on molecular evidence, this result lends further support to the importance of dogs and cats as domestic reservoir hosts of T. cruzi. We believe that in Argentina, this is the first time that lineage IIc has been isolated from naturally infected domestic dogs and Tr. infestans.Fil: Cardinal, Marta Victoria. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; ArgentinaFil: Lauricella, Marta A.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C.G. Malbrán”. Instituto Nacional de Parasitología “Dr. M. Fatala Chabén”; ArgentinaFil: Ceballos, Leonardo A.. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; ArgentinaFil: Lanati, Leonardo. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; ArgentinaFil: Marcet, Paula Lorena. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; ArgentinaFil: Levin, Mariano Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Kitron, Uriel D.. Emory University; Estados UnidosFil: Gurtler, Ricardo Esteban. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; ArgentinaFil: Schijman, Alejandro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentin

Reconfigurable ferromagnetic liquid droplets.

Solid ferromagnetic materials are rigid in shape and cannot be reconfigured. Ferrofluids, although reconfigurable, are paramagnetic at room temperature and lose their magnetization when the applied magnetic field is removed. Here, we show a reversible paramagnetic-to-ferromagnetic transformation of ferrofluid droplets by the jamming of a monolayer of magnetic nanoparticles assembled at the water-oil interface. These ferromagnetic liquid droplets exhibit a finite coercivity and remanent magnetization. They can be easily reconfigured into different shapes while preserving the magnetic properties of solid ferromagnets with classic north-south dipole interactions. Their translational and rotational motions can be actuated remotely and precisely by an external magnetic field, inspiring studies on active matter, energy-dissipative assemblies, and programmable liquid constructs

HST Observations of the Serendipitous X-ray Companion to Mrk 273: Cluster at z=0.46?

We have used HST I-band images to identify Mrk 273X, the very unusual high-redshift X-ray-luminous Seyfert 2 galaxy found by ROSAT in the same field-of-view as Mrk 273. We have measured the photometric properties of Mrk 273X and have also analyzed the luminosity distribution of the faint galaxy population seen in the HST image. The luminosity of the galaxy and the properties of the surrounding environment suggest that Mrk 273X is the brightest galaxy in a relatively poor cluster at a redshift near 0.46. Its off-center location in the cluster and the presence of other galaxy groupings in the HST image may indicate that this is a dynamically young cluster on the verge of merging with its neighboring clusters. We find that Mrk 273X is a bright featureless elliptical galaxy with no evidence for a disk. It follows the de Vaucouleurs (r^{1/4}) surface brightness law very well over a range of 8 magnitudes. Though the surface brightness profile does not appear to be dominated by the AGN, the galaxy has very blue colors that do appear to be produced by the AGN. Mrk 273X is most similar to the IC 5063 class of active galaxies --- a hybrid Sy 2 / powerful radio galaxy.Comment: Accepted for publication in the Astrophysical Journal. 8 pages, including 4 postscript figures. Uses emulateapj.sty and psfig.sty. Higher quality version of Figure 1 is available at http://rings.gsfc.nasa.gov/~borne/fig1-markgals.gi

Vascular Dysfunction in a Transgenic Model of Alzheimer's Disease: Effects of CB1R and CB2R Cannabinoid Agonists

There is evidence of altered vascular function, including cerebrovascular, in Alzheimer's disease (AD) and transgenic models of the disease. Indeed vasoconstrictor responses are increased, while vasodilation is reduced in both conditions. β-Amyloid (Aβ) appears to be responsible, at least in part, of alterations in vascular function. Cannabinoids, neuroprotective and anti-inflammatory agents, induce vasodilation both in vivo and in vitro. We have demonstrated a beneficial effect of cannabinoids in models of AD by preventing glial activation. In this work we have studied the effects of these compounds on vessel density in amyloid precursor protein (APP) transgenic mice, line 2576, and on altered vascular responses in aortae isolated ring. First we showed increased collagen IV positive vessels in AD brain compared to control subjects, with a similar increase in TgAPP mice, which was normalized by prolonged oral treatment with the CB1/CB2 mixed agonist WIN 55,212-2 (WIN) and the CB2 selective agonist JWH-133 (JWH). In Tg APP mice the vasoconstriction induced by phenylephrine and the thromboxane agonist U46619 was significantly increased, and no change in the vasodilation to acetylcholine (ACh) was observed. Tg APP displayed decreased vasodilation to both cannabinoid agonists, which were able to prevent decreased ACh relaxation in the presence of Aβ. In summary, we have confirmed and extended the existence of altered vascular responses in Tg APP mice. Moreover, our results suggest that treatment with cannabinoids may ameliorate the vascular responses in AD-type pathology.This work was supported by the Council of Madrid (S- BIO/0170/2006 and P2010/BMD-2349 to MLC) and by Instituto de Salud Carlos III/FISS (PI12/00590 to TT). AM received a fellowship from the Spanish Ministry of Education and Science and JN-D from FISS. Dr. R. Martínez-Murillo is acknowledged for preliminary EM experiments.Peer reviewedPeer Reviewe

The XMM-Newton serendipitous survey. VII. The third XMM-Newton serendipitous source catalogue

Thanks to the large collecting area (3 x ~1500 cm$^2$ at 1.5 keV) and wide field of view (30' across in full field mode) of the X-ray cameras on board the European Space Agency X-ray observatory XMM-Newton, each individual pointing can result in the detection of hundreds of X-ray sources, most of which are newly discovered. Recently, many improvements in the XMM-Newton data reduction algorithms have been made. These include enhanced source characterisation and reduced spurious source detections, refined astrometric precision, greater net sensitivity and the extraction of spectra and time series for fainter sources, with better signal-to-noise. Further, almost 50\% more observations are in the public domain compared to 2XMMi-DR3, allowing the XMM-Newton Survey Science Centre (XMM-SSC) to produce a much larger and better quality X-ray source catalogue. The XMM-SSC has developed a pipeline to reduce the XMM-Newton data automatically and using improved calibration a new catalogue version has been produced from XMM-Newton data made public by 2013 Dec. 31 (13 years of data). Manual screening ensures the highest data quality. This catalogue is known as 3XMM. In the latest release, 3XMM-DR5, there are 565962 X-ray detections comprising 396910 unique X-ray sources. For the 133000 brightest sources, spectra and lightcurves are provided. For all detections, the positions on the sky, a measure of the quality of the detection, and an evaluation of the X-ray variability is provided, along with the fluxes and count rates in 7 X-ray energy bands, the total 0.2-12 keV band counts, and four hardness ratios. To identify the detections, a cross correlation with 228 catalogues is also provided for each X-ray detection. 3XMM-DR5 is the largest X-ray source catalogue ever produced. Thanks to the large array of data products, it is an excellent resource in which to find new and extreme objects.Comment: 23 pages, version accepted for publication in A&

Radio Loud and Radio Quiet Active Galactic Nuclei

We generated a sample of 409 AGNs for which both the radio luminosity at 5 GHz and the line luminosity in [OIII] 5007 have been measured. The radio luminosity spans a range of ten orders of magnitude, and the [OIII] line luminosity spans a range of eight orders of magnitude --- both considerably larger than the ranges in previous studies. We show that these two quantities are correlated in a similar way for both radio-loud and radio-quiet AGNs. We demonstrate that the observed correlation can be explained in terms of a model in which jets are accelerated and collimated by a vertical magnetic field.Comment: 45 pages inc. 7 figures, 1 table of 15 pages in ps-format. Accept to AJ September 199