Species-specific differences in follicular antral sizes result from diffusion-based limitations on the thickness of the granulosa cell layer

The size of mature oocytes is similar across mammalian species, yet the size of ovarian follicles increases with species size, with some ovarian follicles reaching diameters more than 1000-fold the size of the enclosed oocyte. Here we show that the different follicular sizes can be explained with diffusion-based limitations on the thickness of the hormone-secreting granulosa layer. By analysing published data on human follicular growth and granulosa cell expansion during follicular maturation we find that the 4-fold increase of the antral follicle diameter is entirely driven by an increase in the follicular fluid volume, while the thickness of the surrounding granulosa layer remains constant at about 45+/-10 mkm. Based on the measured kinetic constants, the model reveals that the observed fall in the gonadotropin concentration from peripheral blood circulation to the follicular antrum is a result of sequestration in the granulosa. The model further shows that as a result of sequestration, an increased granulosa thickness cannot substantially increase estradiol production but rather deprives the oocyte from gonadotropins. Larger animals (with a larger blood volume) require more estradiol as produced by the ovaries to downregulate FSH-secretion in the pituitary. Larger follicle diameters result in larger follicle surface areas for constant granulosa layer thickness. The reported increase in follicular surface area in larger species indeed correlates linearly both with species mass and with the predicted increase in estradiol output. In summary, we propose a structural role for the antrum in that it determines the volume of the granulosa layer and thus the level of estrogen production.Comment: Mol Hum Repr 201

A Multifunctional Processing Board for the Fast Track Trigger of the H1 Experiment

The electron-proton collider HERA is being upgraded to provide higher luminosity from the end of the year 2001. In order to enhance the selectivity on exclusive processes a Fast Track Trigger (FTT) with high momentum resolution is being built for the H1 Collaboration. The FTT will perform a 3-dimensional reconstruction of curved tracks in a magnetic field of 1.1 Tesla down to 100 MeV in transverse momentum. It is able to reconstruct up to 48 tracks within 23 mus in a high track multiplicity environment. The FTT consists of two hardware levels L1, L2 and a third software level. Analog signals of 450 wires are digitized at the first level stage followed by a quick lookup of valid track segment patterns. For the main processing tasks at the second level such as linking, fitting and deciding, a multifunctional processing board has been developed by the ETH Zurich in collaboration with Supercomputing Systems (Zurich). It integrates a high-density FPGA (Altera APEX 20K600E) and four floating point DSPs (Texas Instruments TMS320C6701). This presentation will mainly concentrate on second trigger level hardware aspects and on the implementation of the algorithms used for linking and fitting. Emphasis is especially put on the integrated CAM (content addressable memory) functionality of the FPGA, which is ideally suited for implementing fast search tasks like track segment linking.Comment: 6 pages, 4 figures, submitted to TN

Structure of self-assembled Mn atom chains on Si(001)

Mn has been found to self-assemble into atomic chains running perpendicular to the surface dimer reconstruction on Si(001). They differ from other atomic chains by a striking asymmetric appearance in filled state scanning tunneling microscopy (STM) images. This has prompted complicated structural models involving up to three Mn atoms per chain unit. Combining STM, atomic force microscopy and density functional theory we find that a simple necklace-like chain of single Mn atoms reproduces all their prominent features, including their asymmetry not captured by current models. The upshot is a remarkably simpler structure for modelling the electronic and magnetic properties of Mn atom chains on Si(001).Comment: 5 pages, 4 figure