Trehalose is required for the acquisition of tolerance to a variety of stresses in the filamentous fungus Aspergillus nidulans

Trehalose is a non-reducing disaccharide found at high concentrations in Aspergillus nidulans conidia and rapidly degraded upon induction of conidial germination. Furthermore, trehalose is accumulated in response to a heat shock or to an oxidative shock. The authors have characterized the A. nidulans tpsA gene encoding trehalose-6-phosphate synthase, which catalyses the first step in trehalose biosynthesis. Expression of tpsA in a Saccharomyces cerevisiae tps1 mutant revealed that the tpsA gene product is a functional equivalent of the yeast Tps1 trehalose-6-phosphate synthase. The A. nidulans tpsA-null mutant does not produce trehalose during conidiation or in response to various stress conditions. While germlings of the tpsA mutant show an increased sensitivity to moderate stress conditions (growth at 45 °C or in the presence of 2 mM H2O2), they display a response to severe stress (60 min at 50 °C or in the presence of 100 mM H2O2) similar to that of wild-type germlings. Furthermore, conidia of the tpsA mutant show a rapid loss of viability upon storage. These results are consistent with a role of trehalose in the acquisition of stress tolerance. Inactivation of the tpsA gene also results in increased steady-state levels of sugar phosphates but does not prevent growth on rapidly metabolizable carbon sources (glucose, fructose) as seen in Saccharomyces cerevisiae. This suggests that trehalose 6-phosphate is a physiological inhibitor of hexokinase but that this control is not essential for proper glycolytic flux in A. nidulans. Interestingly, tpsA transcription is not induced in response to heat shock or during conidiation, indicating that trehalose accumulation is probably due to a post-translational activation process of the trehalose 6-phosphate synthase

The popular music heritage of the Dutch pirates: illegal radio and cultural identity

This article explores how cultural identities are negotiated in relation to the heritage of illegal radio in the Netherlands. The term ‘pirate radio’ commonly refers to the offshore radio stations that were broadcasting during the 1960s. These stations introduced commercial radio and popular music genres like beat music, which were not played by public broadcasters at the time. In their wake, land-based pirates began broadcasting for local audiences. This study examines the identities that are constituted by the narrative of pirate radio. Drawing on in-depth interviews with archivists, fans and broadcasters, this article explores the connection between pirate radio, popular music heritage and cultural identity. Moreover, it considers how new technologies such as internet radio provide platforms to engage with this heritage and thus to maintain these local identities. To examine how the memories of pirate radio live on in the present a narrative approach to identity will be used

Consumption patterns and living conditions inside Het Steen, the late medieval prison of Malines (Mechelen, Belgium)

Excavations at the Main Square (Grote Markt) of Malines (Mechelen, Belgium) have unearthed the building remains of a tower, arguably identifiable as the former town prison: Het Steen. When this assumption is followed, the contents of the fills of two cesspits dug out in the cellars of the building illustrate aspects of daily life within the early 14th-century prison. An integrated approach of all find categories, together with the historical context available, illuminates aspects of the material culture of the users of the cesspits, their consumption patterns and the living conditions within the building

Platformisation

Este artigo contextualiza, define e operacionaliza o conceito de plataformização. A partir de insights vindos de diferentes perspectivas acadêmicas sobre plataformas – estudos de software, economia política crítica, estudos de negócios e estudos culturais – desenvolvemos uma abordagem compreensiva em relação a esse processo. A plataformização é definida como a penetração de infraestruturas, processos econômicos e estruturas governamentais das plataformas digitais em diferentes setores econômicos e esferas da vida. Ela também envolve a reorganização de práticas e imaginários culturais em torno dessas plataformas. A partir do exemplo de app stores, mostramos como essa definição pode ser empregada em pesquisas empíricas.Palavras-chave: Plataformas. Plataformização. Dataficação. Mercados multilaterais. Economia política. Governança.This article contextualises, defines, and operationalises the concept of platformisation. Drawing insights from different scholarly perspectives on platforms—software studies, critical political economy, business studies, and cultural studies—it develops a comprehensive approach to this process. Platformisation is defined as the penetration of infrastructures, economic processes and governmental frameworks of digital platforms in different economic sectors and spheres of life, as well as the reorganisation of cultural practices and imaginations around these platforms. Using app stores as an example, we show how this definition can be employed in research.Keywords: Platforms. Platformisation. Datafication. Multi-sided markets. Political Economy. Governance

Human cloning in film: horror, ambivalence, hope

Fictional filmic representations of human cloning have shifted in relation to the 1997 announcement of the birth of Dolly the cloned sheep, and since therapeutic human cloning became a scientific practice in the early twentieth century. The operation and detail of these shifts can be seen through an analysis of the films The Island (2005) and Aeon Flux (2005). These films provide a site for the examination of how these changes in human cloning from fiction to practice, and from horror to hope, have been represented and imagined, and how these distinctions have operated visually in fiction, and in relation to genre

Locked down apps versus the social media ecology : why do young people and educators disagree on the best delivery platform for digital sexual health entertainment education?

This article reports on focus groups exploring the best way to reach young men with vulgar comedy videos that provide sexual health information. Young people reported that they found the means by which the material was presented - as a locked down app - to be problematic, and that it would better be delivered through social media platforms such as YouTube. This would make it more 'spreadable'. By contrast, adult sex education stakeholders thought the material should be contained within a locked down, stand-alone app - otherwise it might be seen by children who are too young, and/or young people might misunderstand the messages. We argue that the difference in approach represented by these two sets of opinions represents a fundamental stumbling block for attempts to reach young people with digital sexual health materials, which can be understood through the prism of different cultural forms - education versus entertainment

The narrative self, distributed memory, and evocative objects

In this article, I outline various ways in which artifacts are interwoven with autobiographical memory systems and conceptualize what this implies for the self. I first sketch the narrative approach to the self, arguing that who we are as persons is essentially our (unfolding) life story, which, in turn, determines our present beliefs and desires, but also directs our future goals and actions. I then argue that our autobiographical memory is partly anchored in our embodied interactions with an ecology of artifacts in our environment. Lifelogs, photos, videos, journals, diaries, souvenirs, jewelry, books, works of art, and many other meaningful objects trigger and sometimes constitute emotionally-laden autobiographical memories. Autobiographical memory is thus distributed across embodied agents and various environmental structures. To defend this claim, I draw on and integrate distributed cognition theory and empirical research in human-technology interaction. Based on this, I conclude that the self is neither defined by psychological states realized by the brain nor by biological states realized by the organism, but should be seen as a distributed and relational construct

Social Media and trust in scientific expertise: Debating the Covid-19 Pandemic in The Netherlands

This article examines the role of social media dynamics in the public exchange of information between scientists (experts), government (policy-makers), mass media (journalists), and citizens (nonexperts) during the first 4 months after the Covid-19 outbreak in the Netherlands. Over the past decade, the institutional model of science communication, based on linear vectors of information flows between institutions, has gradually converted into a networked model where social media propel information flows circulating between all actors involved. The question driving our research is, “How are social media deployed to both undermine and enhance public trust in scientific expertise during a health crisis?” Analyzing the public debate during the period of the corona outbreak in the Netherlands, we investigate two stages: the emergency response phase and the “smart exit strategy” phase, discussing how scientific experts, policy-makers, journalists, and citizens appropriate social media logic to steer information and to control the debate. We conclude by outlining the potential risks and benefits of adopting social media dynamics in institutional contexts of science communication