Infrared dust emission in the outer disk of M51

We examine faint infrared emission features detected in Spitzer Space Telescope images of M51, which are associated with atomic hydrogen in the outer disk and tidal tail at R greater than R_25 (4.9', ~14 kpc at d=9.6 Mpc). The infrared colors of these features are consistent with the colors of dust associated with star formation in the bright disk. However, the star formation efficiency (as a ratio of star formation rate to neutral gas mass) implied in the outer disk is lower than that in the bright disk of M51 by an order of magnitude, assuming a similar relationship between infrared emission and star formation rate in the inner and outer disks.Comment: 13 pages in manuscript form, 2 figures; download PDF of manuscript with original-resolution Figure 1 at http://www.eg.bucknell.edu/physics/thornley/thornleym51.pd

Metal production in M33: space and time variations

Nearby galaxies are ideal places to study in detail metallicity gradients and their time evolution. We consider chemical abundances of a new sample of \hii\ regions complemented with previous literature data-sets. We compare \hii\ region and PN abundances obtained with a common set of observations taken at MMT. With an updated theoretical model, we follow the time evolution of the baryonic components and chemical abundances in the disk of M33, assuming that the galaxy is accreting gas from an external reservoir. Supported by a uniform sample of nebular spectroscopic observations, we conclude that: {\em i}) the metallicity distribution in M33 is very complex, showing a central depression in metallicity probably due to observational bias; {\em ii}) the metallicity gradient in the disk of M33 has a slope of -0.037$\pm$ 0.009 dex kpc$^{-1}$ in the whole radial range up to $\sim$8 kpc, and -0.044$\pm$ 0.009 dex kpc$^{-1}$ excluding the central kpc; {\em iii}) there is a small evolution of the slope with time from the epoch of PN progenitor formation to the present-time.}Comment: A&A accepted, 15 Pags, 13 Figs, language correctio

Discovery of a detached HI gas shell surrounding alpha Orionis

We report the detection of the HI line at 21 cm in the direction of alpha Ori with the Nancay Radiotelescope and with the Very Large Array. The observations confirm the previous detection of HI emission centered on alpha Ori, but additionally reveal for the first time a quasi-stationary detached shell of neutral atomic hydrogen ~4 arcmin. in diameter (0.24 pc at a distance of 200 pc). The detached shell appears elongated in a direction opposite to the star's space motion. A simple model shows that this detached atomic gas shell can result from the collision of the stellar wind from alpha Ori with the local interstellar medium (ISM). It implies that alpha Ori has been losing matter at a rate of ~ 1.2x10^-6 solar masses per year for the past 8x10^4 years. In addition, we report the detection of atomic hydrogen associated with the far-infrared arc located 6 arcmin. north-east of alpha Ori, that has been suggested to trace the bow shock resulting from the motion of the star through the ISM. We report also the detection by the Galaxy Evolution Explorer (GALEX) of a far-UV counterpart to this arc.Comment: Accepted for publication in the Monthly Notices; version with full resolution figures available at http://aramis.obspm.fr/~lebertre/paper-alphaOri_MNRAS.pd

Cool gas and dust in M33: Results from the Herschel M33 extended survey (HERM33ES)

We present an analysis of the first space-based far-IR-submm observations of M 33, which measure the emission from the cool dust and resolve the giant molecular cloud complexes. With roughly half-solar abundances, M33 is a first step towards young low-metallicity galaxies where the submm may be able to provide an alternative to CO mapping to measure their H$_2$ content. In this Letter, we measure the dust emission cross-section $\sigma$ using SPIRE and recent CO and \HI\ observations; a variation in $\sigma$ is present from a near-solar neighborhood cross-section to about half-solar with the maximum being south of the nucleus. Calculating the total H column density from the measured dust temperature and cross-section, and then subtracting the \HI\ column, yields a morphology similar to that observed in CO. The H$_2$/\HI\ mass ratio decreases from about unity to well below 10% and is about 15% averaged over the optical disk. The single most important observation to reduce the potentially large systematic errors is to complete the CO mapping of M 33.Comment: 5 pages, 5 figures Accepted for publication in Astronomy and Astrophysic

X Her and TX Psc: Two cases of ISM interaction with stellar winds observed by Herschel

The asymptotic giant branch (AGB) stars X Her and TX Psc have been imaged at 70 and 160 microns with the PACS instrument onboard the Herschel satellite, as part of the large MESS (Mass loss of Evolved StarS) Guaranteed Time Key Program. The images reveal an axisymmetric extended structure with its axis oriented along the space motion of the stars. This extended structure is very likely to be shaped by the interaction of the wind ejected by the AGB star with the surrounding interstellar medium (ISM). As predicted by numerical simulations, the detailed structure of the wind-ISM interface depends upon the relative velocity between star+wind and the ISM, which is large for these two stars (108 and 55 km/s for X Her and TX Psc, respectively). In both cases, there is a compact blob upstream whose origin is not fully elucidated, but that could be the signature of some instability in the wind-ISM shock. Deconvolved images of X Her and TX Psc reveal several discrete structures along the outermost filaments, which could be Kelvin-Helmholtz vortices. Finally, TX Psc is surrounded by an almost circular ring (the signature of the termination shock?) that contrasts with the outer, more structured filaments. A similar inner circular structure seems to be present in X Her as well, albeit less clearly.Comment: 11 pages, Astronomy & Astrophysics, in pres

Rescue of Dystrophic Skeletal Muscle by PGC-1α Involves a Fast to Slow Fiber Type Shift in the mdx Mouse

Increased utrophin expression is known to reduce pathology in dystrophin-deficient skeletal muscles. Transgenic over-expression of PGC-1α has been shown to increase levels of utrophin mRNA and improve the histology of mdx muscles. Other reports have shown that PGC-1α signaling can lead to increased oxidative capacity and a fast to slow fiber type shift. Given that it has been shown that slow fibers produce and maintain more utrophin than fast skeletal muscle fibers, we hypothesized that over-expression of PGC-1α in post-natal mdx mice would increase utrophin levels via a fiber type shift, resulting in more slow, oxidative fibers that are also more resistant to contraction-induced damage. To test this hypothesis, neonatal mdx mice were injected with recombinant adeno-associated virus (AAV) driving expression of PGC-1α. PGC-1α over-expression resulted in increased utrophin and type I myosin heavy chain expression as well as elevated mitochondrial protein expression. Muscles were shown to be more resistant to contraction-induced damage and more fatigue resistant. Sirt-1 was increased while p38 activation and NRF-1 were reduced in PGC-1α over-expressing muscle when compared to control. We also evaluated if the use a pharmacological PGC-1α pathway activator, resveratrol, could drive the same physiological changes. Resveratrol administration (100 mg/kg/day) resulted in improved fatigue resistance, but did not achieve significant increases in utrophin expression. These data suggest that the PGC-1α pathway is a potential target for therapeutic intervention in dystrophic skeletal muscle

100 mum and 160 mum emission as resolved star-formation rate estimators in M33 (HERM33ES)

Over the past few years several studies have provided estimates of the SFR (star-formation rate) or the total infrared luminosity from just one infrared band. However these relations are generally derived for entire galaxies, which are known to contain a large scale diffuse emission that is not necessarily related to the latest star-formation episode. We provide new relations to estimate the SFR from resolved star-forming regions at 100 mum and 160 mum. We select individual star-forming regions in the nearby (840 kpc) galaxy M33. We estimate the SFR combining the emission in Halpha and at 24 mum to calibrate the emission at 100 mum and 160 mum as SFR estimators, as mapped with PACS/Herschel. The data are obtained in the framework of the HERM33ES open time key project. There is less emission in the HII regions at 160 mum than at 100 mum. Over a dynamic range of almost 2 dex in Sigma(SFR) we find that the 100 mum emission is a nearly linear estimator of the SFR, whereas that at 160 mum is slightly superlinear. The behaviour of individual star-forming regions is surprisingly similar to that of entire galaxies. At high Sigma(SFR), star formation drives the dust temperature, whereas uncertainties and variations in radiation-transfer and dust-heated processes dominate at low Sigma(SFR). Detailed modelling of both galaxies and individual star forming regions will be needed to interpret similarities and differences between the two and assess the fraction of diffuse emission in galaxies.Comment: 5 pages, 3 figures, accepted for publication in the A&A Herschel special issu

Genome Sequence Analyses of Pseudomonas savastanoi pv. glycinea and Subtractive Hybridization-Based Comparative Genomics with Nine Pseudomonads

Bacterial blight, caused by Pseudomonas savastanoi pv. glycinea (Psg), is a common disease of soybean. In an effort to compare a current field isolate with one isolated in the early 1960s, the genomes of two Psg strains, race 4 and B076, were sequenced using 454 pyrosequencing. The genomes of both Psg strains share more than 4,900 highly conserved genes, indicating very low genetic diversity between Psg genomes. Though conserved, genome rearrangements and recombination events occur commonly within the two Psg genomes. When compared to each other, 437 and 163 specific genes were identified in B076 and race 4, respectively. Most specific genes are plasmid-borne, indicating that acquisition and maintenance of plasmids may represent a major mechanism to change the genetic composition of the genome and even acquire new virulence factors. Type three secretion gene clusters of Psg strains are near identical with that of P. savastanoi pv. phaseolicola (Pph) strain 1448A and they shared 20 common effector genes. Furthermore, the coronatine biosynthetic cluster is present on a large plasmid in strain B076, but not in race 4. In silico subtractive hybridization-based comparative genomic analyses with nine sequenced phytopathogenic pseudomonads identified dozens of specific islands (SIs), and revealed that the genomes of Psg strains are more similar to those belonging to the same genomospecies such as Pph 1448A than to other phytopathogenic pseudomonads. The number of highly conserved genes (core genome) among them decreased dramatically when more genomes were included in the subtraction, suggesting the diversification of pseudomonads, and further indicating the genome heterogeneity among pseudomonads. However, the number of specific genes did not change significantly, suggesting these genes are indeed specific in Psg genomes. These results reinforce the idea of a species complex of P. syringae and support the reclassification of P. syringae into different species

Quorum Sensing Signaling Molecules Produced by Reference and Emerging Soft-Rot Bacteria (Dickeya and Pectobacterium spp.)

International audienceBACKGROUND: Several small diffusible molecules are involved in bacterial quorum sensing and virulence. The production of autoinducers-1 and -2, quinolone, indole and γ-amino butyrate signaling molecules was investigated in a set of soft-rot bacteria belonging to six Dickeya or Pectobacterium species including recent or emerging potato isolates. METHODOLOGY/PRINCIPAL FINDINGS: Using bacterial biosensors, immunoassay, and chromatographic analysis, we showed that soft-rot bacteria have the common ability to produce transiently during their exponential phase of growth the N-3-oxo-hexanoyl- or the N-3-oxo-octanoyl-l-homoserine lactones and a molecule of the autoinducer-2 family. Dickeya spp. produced in addition the indole-3-acetic acid in tryptophan-rich conditions. All these signaling molecules have been identified for the first time in the novel Dickeya solani species. In contrast, quinolone and γ-amino butyrate signals were not identified and the corresponding synthases are not present in the available genomes of soft-rot bacteria. To determine if the variations of signal production according to growth phase could result from expression modifications of the corresponding synthase gene, the respective mRNA levels were estimated by reverse transcriptase-PCR. While the N-acyl-homoserine lactone production is systematically correlated to the synthase expression, that of the autoinducer-2 follows the expression of an enzyme upstream in the activated methyl cycle and providing its precursor, rather than the expression of its own synthase. CONCLUSIONS/SIGNIFICANCE: Despite sharing the S-adenosylmethionine precursor, no strong link was detected between the production kinetics or metabolic pathways of autoinducers-1 and -2. In contrast, the signaling pathway of autoinducer-2 seems to be switched off by the indole-3-acetic acid pathway under tryptophan control. It therefore appears that the two genera of soft-rot bacteria have similarities but also differences in the mechanisms of communication via the diffusible molecules. Our results designate autoinducer-1 lactones as the main targets for a global biocontrol of soft-rot bacteria communications, including those of emerging isolates