Health and sustainable development

If sustainable development is to mean anything, people must be healthy enough to benefit from it and not have their lives cut off prematurely. Development without health is meaningless. But the processes which are likely to occur in a world undergoing globalisation, climate change, urbanisation, population increase and many other changes, will impact upon human health in complex ways. Some of them will benefit us, others will create new or augmented threats to survival and health, while many others will have a complex mixture of effects

Peripheral visual response time to colored stimuli imaged on the horizontal meridian

Two male observers were administered a binocular visual response time task to small (45 min arc), flashed, photopic stimuli at four dominant wavelengths (632 nm red; 583 nm yellow; 526 nm green; 464 nm blue) imaged across the horizontal retinal meridian. The stimuli were imaged at 10 deg arc intervals from 80 deg left to 90 deg right of fixation. Testing followed either prior light adaptation or prior dark adaptation. Results indicated that mean response time (RT) varies with stimulus color. RT is faster to yellow than to blue and green and slowest to red. In general, mean RT was found to increase from fovea to periphery for all four colors, with the curve for red stimuli exhibiting the most rapid positive acceleration with increasing angular eccentricity from the fovea. The shape of the RT distribution across the retina was also found to depend upon the state of light or dark adaptation. The findings are related to previous RT research and are discussed in terms of optimizing the color and position of colored displays on instrument panels

Decoupled freshwater transport and meridional overturning in the South Atlantic

Freshwater transports (F_ov) by the Atlantic Meridional Overturning Circulation (AMOC) are sensitive to salinity distributions and may determine AMOC stability. However, climate models show large salinity biases, distorting the relation between F_ov and the AMOC. Using free-running models and ocean reanalyses with realistic salinities but quite different AMOCs, we show that the fresh Antarctic Intermediate Water (AAIW) layer eliminates salinity differences across the AMOC branches at ~1200 m, ∆S_1200m, which decouples F_ov from the AMOC south of ~10˚N. As AAIW disappears north of ~10˚N, a large ∆S_1200m allows the AMOC to drive substantial southward F_ov in the North Atlantic. In the South Atlantic the 0-300 m zonal salinity contrasts control the gyre freshwater transports F_gyre, which also determine the total freshwater transports. This decoupling makes the southern F_ov unlikely to play any role in AMOC stability, leaving indirect F_gyre feedbacks or F_ov in the north, as more relevant factors

Anatomy, morphology and evolution of the patella in squamate lizards and tuatara (Sphenodon punctatus)

The patella (kneecap) is the largest and best-known of the sesamoid bones, postulated to confer biomechanical advantages including increasing joint leverage and reinforcing the tendon against compression. It has evolved several times independently in amniotes, but despite apparently widespread occurrence in lizards, the patella remains poorly characterised in this group and is, as yet, completely undescribed in their nearest extant relative Sphenodon (Rhynchocephalia). Through radiography, osteological and fossil studies we examined patellar presence in diverse lizard and lepidosauromorph taxa, and using computed tomography, dissection and histology we investigated in greater depth the anatomy and morphology of the patella in 16 lizard species and 19 Sphenodon specimens. We have found the first unambiguous evidence of a mineralised patella in Sphenodon, which appears similar to the patella of lizards and shares several gross and microscopic anatomical features. Although there may be a common mature morphology, the squamate patella exhibits a great deal of variability in development (whether from a cartilage anlage or not, and in the number of mineralised centres) and composition (bone, mineralised cartilage or fibrotendinous tissue). Unlike in mammals and birds, the patella in certain lizards and Sphenodon appears to be a polymorphic trait. We have also explored the evolution of the patella through ancestral state reconstruction, finding that the patella is ancestral for lizards and possibly Lepidosauria as a whole. Clear evidence of the patella in rhynchocephalian or stem lepidosaurian fossil taxa would clarify the evolutionary origin(s) of the patella, but due to the small size of this bone and the opportunity for degradation or loss we could not definitively conclude presence or absence in the fossils examined. The pattern of evolution in lepidosaurs is unclear but our data suggest that the emergence of this sesamoid may be related to the evolution of secondary ossification centres and/or changes in knee joint conformation, where enhancement of extensor muscle leverage would be more beneficial.Sophie Regnault, Marc E. H. Jones, Andrew A. Pitsillides, John R. Hutchinso

Study of an attitude control system for the astronaut maneuvering unit final report, dec. 1963 - jul. 1964

Attitude control system for astronaut maneuvering unit

Shapley Supercluster Survey: Ram-Pressure Stripping vs. Tidal Interactions in the Shapley Supercluster

We present two new examples of galaxies undergoing transformation in the Shapley supercluster core. These low-mass (stellar mass from 0.4E10 to 1E10 Msun) galaxies are members of the two clusters SC-1329-313 (z=0.045) and SC-1327-312 (z=0.049). Integral-field spectroscopy complemented by imaging in ugriK bands and in Halpha narrow-band are used to disentangle the effects of tidal interaction (TI) and ram-pressure stripping (RPS). In both galaxies, SOS-61086 and SOS-90630, we observe one-sided extraplanar ionized gas extending respectively 30kpc and 41kpc in projection from their disks. The galaxies' gaseous disks are truncated and the kinematics of the stellar and gas components are decoupled, supporting the RPS scenario. The emission of the ionized gas extends in the direction of a possible companion for both galaxies suggesting a TI. The overall gas velocity field of SOS-61086 is reproduced by ad hoc N-body/hydrodynamical simulations of RPS acting almost face-on and starting about 250Myr ago, consistent with the age of the young stellar populations. A link between the observed gas stripping and the cluster-cluster interaction experienced by SC-1329-313 and A3562 is suggested. Simulations of ram pressure acting almost edge-on are able to fully reproduce the gas velocity field of SOS-90630, but cannot at the same time reproduce the extended tail of outflowing gas. This suggests that an additional disturbance from a TI is required. This study adds a piece of evidence that RPS may take place in different environments with different impacts and witnesses the possible effect of cluster-cluster merger on RPS.Comment: 27 pages, 28 figures, MNRAS accepte

Fatty-acid uptake in prostate cancer cells using dynamic microfluidic raman technology

It is known that intake of dietary fatty acid (FA) is strongly correlated with prostate cancer progression but is highly dependent on the type of FAs. High levels of palmitic acid (PA) or arachidonic acid (AA) can stimulate the progression of cancer. In this study, a unique experimental set-up consisting of a Raman microscope, coupled with a commercial shear-flow microfluidic system is used to monitor fatty acid uptake by prostate cancer (PC-3) cells in real-time at the single cell level. Uptake of deuterated PA, deuterated AA, and the omega-3 fatty acids docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) were monitored using this new system, while complementary flow cytometry experiments using Nile red staining, were also conducted for the validation of the cellular lipid uptake. Using this novel experimental system, we show that DHA and EPA have inhibitory effects on the uptake of PA and AA by PC-3 cells

Substrate proteolysis is inhibited by dominant-negative Nedd4 and Rsp5 mutants harboring alterations in WW domain 1

Mammalian Nedd4 and its budding yeast orthologue Rsp5 are members of a large family of HECT-domain-containing ubiquitin ligases. Besides possessing a Ca2+/lipid-binding domain, both ligases have multiple protein-interacting modules termed WW domains. The C-terminal WW domains mediate interactions with substrates, but the function of the first WW domain remains unclear. We found that expression of a WW domain 1 Nedd4 mutant inhibits the growth of budding yeast by affecting the rsp5-ole1 pathway. The WW domain 1 mutant-induced phenotype is suppressed by ole1 cDNA overexpression or oleic acid supplementation of growth media and ole1 RNA levels are reduced in cells expressing this Nedd4 mutant. Also, the WW domain 1 Nedd4 mutant associates via WW domains 2 and 3 with Spt23, a Rsp5 target and ole1 transactivator. The dominant-negative activity of this mutant is associated with promoting accumulation of unprocessed Spt23 and inhibiting generation of processed and presumably active protein. Also, Spt23 processing is inhibited by a Nedd4 mutant that lacks ubiquitin ligase activity and Spt23-binding-competent Rsp5 mutants harboring WW domain 1 or ligase domain mutations. Interestingly, in mammalian cells, wild-type Nedd4 promotes proteasome-mediated degradation of the precursor form of Spt23. WW domain 1 and ligase domain Nedd4 mutants block its degradation. These results indicate that WW domain 1 of these ligases interacts with cofactors that are required for ubiquitin/proteasome-dependent proteolysis of bound substrates.Natalia Shcherbik, Sharad Kumar and Dale S. Haine

Testing the Modern Merger Hypothesis via the Assembly of Massive Blue Elliptical Galaxies in the Local Universe

The modern merger hypothesis offers a method of forming a new elliptical galaxy through merging two equal-mass, gas-rich disk galaxies fuelling a nuclear starburst followed by efficient quenching and dynamical stabilization. A key prediction of this scenario is a central concentration of young stars during the brief phase of morphological transformation from highly-disturbed remnant to new elliptical galaxy. To test this aspect of the merger hypothesis, we use integral field spectroscopy to track the stellar Balmer absorption and 4000\AA\ break strength indices as a function of galactic radius for 12 massive (${\rm M_{*}}\ge10^{10}{\rm M_{\odot}}$), nearby (${\rm z}\le0.03$), visually-selected plausible new ellipticals with blue-cloud optical colours and varying degrees of morphological peculiarities. We find that these index values and their radial dependence correlate with specific morphological features such that the most disturbed galaxies have the smallest 4000\AA\ break strengths and the largest Balmer absorption values. Overall, two-thirds of our sample are inconsistent with the predictions of the modern merger hypothesis. Of these eight, half exhibit signatures consistent with recent minor merger interactions. The other half have star formation histories similar to local, quiescent early-type galaxies. Of the remaining four galaxies, three have the strong morphological disturbances and star-forming optical colours consistent with being remnants of recent, gas-rich major mergers, but exhibit a weak, central burst consistent with forming $\sim5\%$ of their stars. The final galaxy possesses spectroscopic signatures of a strong, centrally-concentrated starburst and quiescent core optical colours indicative of recent quenching (i.e., a post-starburst signature) as prescribed by the modern merger hypothesis.Comment: 25 pages, 37 figures, accepted to MNRA