Basal rot of narcissus : understanding pathogenicity in fusarium oxysporum f. sp. narcissi

Fusarium oxysporum is a globally distributed soilborne fungal pathogen causing root rots, bulb rots, crown rots and vascular wilts on a range of horticultural plants. Pathogenic F. oxysporum isolates are highly host specific and are classified as formae speciales. Narcissus is an important ornamental crop and both the quality and yield of flowers and bulbs can be severely affected by a basal rot caused by F. oxysporum f. sp. narcissi (FON); 154 Fusarium isolates were obtained from different locations and Narcissus cultivars in the United Kingdom, representing a valuable resource. A subset of 30 F. oxysporum isolates were all found to be pathogenic and were therefore identified as FON. Molecular characterisation of isolates through sequencing of three housekeeping genes, suggested a monophyletic origin with little divergence. PCR detection of 14 Secreted in Xylem (SIX) genes, previously shown to be associated with pathogenicity in other F. oxysporum f. spp., revealed different complements of SIX7, SIX9, SIX10, SIX12 and SIX13 within FON isolates which may suggest a race structure. SIX gene sequences were unique to FON and SIX10 was present in all isolates, allowing for molecular identification of FON for the first time. The genome of a highly pathogenic isolate was sequenced and lineage specific (LS) regions identified which harboured putative effectors including the SIX genes. Real-time RT-PCR, showed that SIX genes and selected putative effectors were expressed in planta with many significantly upregulated during infection. This is the first study to characterise molecular variation in FON and provide an analysis of the FON genome. Identification of expressed genes potentially associated with virulence provides the basis for future functional studies and new targets for molecular diagnostics

Traffic jams and intermittent flows in microfluidic networks

We investigate both experimentally and theoretically the traffic of particles flowing in microfluidic obstacle networks. We show that the traffic dynamics is a non-linear process: the particle current does not scale with the particle density even in the dilute limit where no particle collision occurs. We demonstrate that this non-linear behavior stems from long range hydrodynamic interactions. Importantly, we also establish that there exists a maximal current above which no stationary particle flow can be sustained. For higher current values, intermittent traffic jams form thereby inducing the ejection of the particles from the initial path and the subsequent invasion of the network. Eventually, we put our findings in the broader context of the transport proccesses of driven particles in low dimension

Influence of flow confinement on the drag force on a static cylinder

The influence of confinement on the drag force $F$ on a static cylinder in a viscous flow inside a rectangular slit of aperture $h_0$ has been investigated from experimental measurements and numerical simulations. At low enough Reynolds numbers, $F$ varies linearly with the mean velocity and the viscosity, allowing for the precise determination of drag coefficients $\lambda_{||}$ and $\lambda_{\bot}$ corresponding respectively to a mean flow parallel and perpendicular to the cylinder length $L$. In the parallel configuration, the variation of $\lambda_{||}$ with the normalized diameter $\beta = d/h_0$ of the cylinder is close to that for a 2D flow invariant in the direction of the cylinder axis and does not diverge when $\beta = 1$. The variation of $\lambda_{||}$ with the distance from the midplane of the model reflects the parabolic Poiseuille profile between the plates for $\beta \ll 1$ while it remains almost constant for $\beta \sim 1$. In the perpendicular configuration, the value of $\lambda_{\bot}$ is close to that corresponding to a 2D system only if $\beta \ll 1$ and/or if the clearance between the ends of the cylinder and the side walls is very small: in that latter case, $\lambda_{\bot}$ diverges as $\beta \to 1$ due to the blockage of the flow. In other cases, the side flow between the ends of the cylinder and the side walls plays an important part to reduce $\lambda_{\bot}$: a full 3D description of the flow is needed to account for these effects

Experimental and numerical investigations of flow structure and momentum transport in a turbulent buoyancy-driven flow inside a tilted tube.

Buoyancy-driven turbulent mixing of fluids of slightly different densities [At = Δρ/(2〈ρ〉) = 1.15×10−2] in a long circular tube tilted at an angle θ = 15° from the vertical is studied at the local scale, both experimentally from particle image velocimetry and laser induced fluorescence measurements in the vertical diametrical plane and numerically throughout the tube using direct numerical simulation. In a given cross section of the tube, the axial mean velocity and the mean concentration both vary linearly with the crosswise distance z from the tube axis in the central 70% of the diameter. A small crosswise velocity component is detected in the measurement plane and is found to result from a four-cell mean secondary flow associated with a nonzero streamwise component of the vorticity. In the central region of the tube cross section, the intensities of the three turbulent velocity fluctuations are found to be strongly different, that of the streamwise fluctuation being more than twice larger than that of the spanwise fluctuation which itself is about 50% larger than that of the crosswise fluctuation. This marked anisotropy indicates that the turbulent structure is close to that observed in homogeneous turbulent shear flows. Still in the central region, the turbulent shear stress dominates over the viscous stress and reaches a maximum on the tube axis. Its crosswise variation is approximately accounted for by a mixing length whose value is about one-tenth of the tube diameter. The momentum exchange in the core of the cross section takes place between its lower and higher density parts and there is no net momentum exchange between the core and the near-wall regions. A sizable part of this transfer is due both to the mean secondary flow and to the spanwise turbulent shear stress. Near-wall regions located beyond the location of the extrema of the axial velocity (|z|≳0.36 d) are dominated by viscous stresses which transfer momentum toward (from) the wall near the top (bottom) of the tube

Spin splitting in a polarized quasi-two-dimensional exciton gas

We have observed a large spin splitting between "spin" $+1$ and $-1$ heavy-hole excitons, having unbalanced populations, in undoped GaAs/AlAs quantum wells in the absence of any external magnetic field. Time-resolved photoluminescence spectroscopy, under excitation with circularly polarized light, reveals that, for high excitonic density and short times after the pulsed excitation, the emission from majority excitons lies above that of minority ones. The amount of the splitting, which can be as large as 50% of the binding energy, increases with excitonic density and presents a time evolution closely connected with the degree of polarization of the luminescence. Our results are interpreted on the light of a recently developed model, which shows that, while intra-excitonic exchange interaction is responsible for the spin relaxation processes, exciton-exciton interaction produces a breaking of the spin degeneracy in two-dimensional semiconductors.Comment: Revtex, four pages; four figures, postscript file Accepted for publication in Physical Review B (Rapid Commun.

The significance of seniority for women managers’ interpretations of organizational restructuring

This paper examines the impact of restructuring within the transport and logistics sector on women managers working at senior and less senior (middle/junior management) levels of the organization. The majority of women experienced increased performance pressures and heavier workloads as well as an increase in working hours. At the same time, there were pressures to work at home (i.e. week-ends and evenings) and reduced opportunities to work from home (i.e. during normal office hours). Management level emerged as an important factor in how these changes were interpreted. Senior managers perceived more positive outcomes in terms of increased motivation and loyalty. Despite a longer working week, they were less likely to report low morale as an outcome from long hours. In fact, irrespective of management level, women working shorter hours were more likely to report low morale as an outcome. Results are discussed in relation to literature on restructuring and careers, in terms of perceptual framing and in relation to different levels of investment in the organization

Fine structure of excitons in Cu2_2O

Three experimental observations on 1s-excitons in Cu$_2$O are not consistent with the picture of the exciton as a simple hydrogenic bound state: the energies of the 1s-excitons deviate from the Rydberg formula, the total exciton mass exceeds the sum of the electron and hole effective masses, and the triplet-state excitons lie above the singlet. Incorporating the band structure of the material, we calculate the corrections to this simple picture arising from the fact that the exciton Bohr radius is comparable to the lattice constant. By means of a self-consistent variational calculation of the total exciton mass as well as the ground-state energy of the singlet and the triplet-state excitons, we find excellent agreement with experiment.Comment: Revised abstract; 10 pages, revtex, 3 figures available from G. Kavoulakis, Physics Department, University of Illinois, Urban

Quantum saturation and condensation of excitons in Cu2_2O: a theoretical study

Recent experiments on high density excitons in Cu$_2$O provide evidence for degenerate quantum statistics and Bose-Einstein condensation of this nearly ideal gas. We model the time dependence of this bosonic system including exciton decay mechanisms, energy exchange with phonons, and interconversion between ortho (triplet-state) and para (singlet-state) excitons, using parameters for the excitonic decay, the coupling to acoustic and low-lying optical phonons, Auger recombination, and ortho-para interconversion derived from experiment. The single adjustable parameter in our model is the optical-phonon cooling rate for Auger and laser-produced hot excitons. We show that the orthoexcitons move along the phase boundary without crossing it (i.e., exhibit a ``quantum saturation''), as a consequence of the balance of entropy changes due to cooling of excitons by phonons and heating by the non-radiative Auger two-exciton recombination process. The Auger annihilation rate for para-para collisions is much smaller than that for ortho-para and ortho-ortho collisions, explaining why, under the given experimental conditions, the paraexcitons condense while the orthoexcitons fail to do so.Comment: Revised to improve clarity and physical content 18 pages, revtex, figures available from G. Kavoulakis, Physics Department, University of Illinois, Urban

Disparities and guideline adherence in drugs of abuse screening in intracerebral hemorrhage

OBJECTIVE: To characterize the pattern of urine drug screening in a cohort of intracerebral hemorrhage (ICH) patients at our academic centers. METHODS: We identified cases of primary ICH occurring from 2009 to 2011 in our academic centers. Demographic data, imaging characteristics, processes of care, and short-term outcomes were ascertained. We performed logistic regression to identify predictors for screening and evaluated preguideline and postguideline reiteration screening patterns. RESULTS: We identified 610 patients with primary ICH in 2009-2011; 379 (62.1%) were initially evaluated at an outside hospital. Overall, 142/610 (23.3%) patients were screened, with 21 positive for cocaine and 3 for amphetamine. Of patients <55 years of age, only 65/140 (46.4%) were screened. Black patients <55 years of age were screened more than nonblack patients <55 years of age (38/61 [62.3%] vs 27/79 [34.2%]; p = 0.0009). In the best multivariable model, age group (p = 0.0001), black race (p = 0.4529), first Glasgow Coma Scale score (p = 0.0492), current smoking (p < 0.0001), and age group × black race (p = 0.0097) were associated with screening. Guideline reiteration in 2010 did not improve the proportion <55 years of age who were screened: 42/74 (56.8%) were screened before and 23/66 (34.9%) after (p = 0.01). CONCLUSIONS: We found disparities in drugs of abuse (DOA) screening and suboptimal guideline adherence. Systematic efforts to improve screening for DOA are warranted. Improved identification of sympathomimetic exposure may improve etiologic classification and influence decision-making and prognosis counseling

Management control – International Observatory 2014

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