389 research outputs found

    Miniature electrooptical air flow sensor

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    A sensor for measuring flow direction and airspeed that is suitable, because of its small size, for rapid instrumentation of research airplanes is described. A propeller driven sphere rotating at a speed proportional to airspeed presents a reflective target to an electro-optical system such that the duty cycle of the resulting electrical output is proportional to yaw angle and the frequency is proportional to airspeed

    A suspended anemometer system for measuring true airspeed on low-speed airplanes

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    A suspended anemometer system for calibrating pitot-static systems on low speed research airplanes is described. The anemometer measures true airspeed when suspended beneath the airplane on a long cable in regions of undisturbed air. The electrical output of the propeller driven tachometer is a sine wave, the frequency of which is proportional to true airspeed. The anemometer measures true airspeed over a range from 20 to 60 m/sec at altitudes to 3000 m, with an accuracy of + or - 0.5 percent of full scale range. This accuracy is exclusive of errors in the recording system. The stability of the suspended system was investigated and was found adequate in the airspeed range. For the purpose of determining the location of the anemometer relative to the airplane, a method is given for calculating the shape assumed by the deployed cable

    Miniature flow-direction and airspeed sensor for airplanes and radio controlled models in spin studies

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    A miniature flow direction and airspeed sensor was developed for use on 1/10- to 1/15 scale models and on full-scale airplanes engaged in spin research. The range of flow angles encountered in spinning flight (+ or - 120 degrees in angle of attack and + or - 55 degrees in sideslip) is larger than that of normal flight. These angles, along with an effective airspeed range of 9 to 90 m/sec, were measured with static accuracies of + or - 0.35 degrees for angle of attack, + or - 0.25 degrees for sideslip angle, and + or - 1 m/sec for airspeed. The dynamic accuracy is adequate to measure the rapidly changing flow angles and airspeed without singificant distortion. The sensor is rugged enough to withstand both the airplane environment and that of the radio-controlled, unpowered models

    The Restoration Handbook

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    https://digitalcommons.acu.edu/crs_books/1111/thumbnail.jp

    Generalized Interpolation Material Point Approach to High Melting Explosive with Cavities Under Shock

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    Criterion for contacting is critically important for the Generalized Interpolation Material Point(GIMP) method. We present an improved criterion by adding a switching function. With the method dynamical response of high melting explosive(HMX) with cavities under shock is investigated. The physical model used in the present work is an elastic-to-plastic and thermal-dynamical model with Mie-Gr\"uneissen equation of state. We mainly concern the influence of various parameters, including the impacting velocity vv, cavity size RR, etc, to the dynamical and thermodynamical behaviors of the material. For the colliding of two bodies with a cavity in each, a secondary impacting is observed. Correspondingly, the separation distance DD of the two bodies has a maximum value DmaxD_{\max} in between the initial and second impacts. When the initial impacting velocity vv is not large enough, the cavity collapses in a nearly symmetric fashion, the maximum separation distance DmaxD_{\max} increases with vv. When the initial shock wave is strong enough to collapse the cavity asymmetrically along the shock direction, the variation of DmaxD_{\max} with vv does not show monotonic behavior. Our numerical results show clear indication that the existence of cavities in explosive helps the creation of ``hot spots''.Comment: Figs.2,4,7,11 in JPG format; Accepted for publication in J. Phys. D: Applied Physic

    Cross-site comparison of ribosomal depletion kits for Illumina RNAseq library construction

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    Background Ribosomal RNA (rRNA) comprises at least 90% of total RNA extracted from mammalian tissue or cell line samples. Informative transcriptional profiling using massively parallel sequencing technologies requires either enrichment of mature poly-adenylated transcripts or targeted depletion of the rRNA fraction. The latter method is of particular interest because it is compatible with degraded samples such as those extracted from FFPE and also captures transcripts that are not poly-adenylated such as some non-coding RNAs. Here we provide a cross-site study that evaluates the performance of ribosomal RNA removal kits from Illumina, Takara/Clontech, Kapa Biosystems, Lexogen, New England Biolabs and Qiagen on intact and degraded RNA samples. Results We find that all of the kits are capable of performing significant ribosomal depletion, though there are differences in their ease of use. All kits were able to remove ribosomal RNA to below 20% with intact RNA and identify ~ 14,000 protein coding genes from the Universal Human Reference RNA sample at >1FPKM. Analysis of differentially detected genes between kits suggests that transcript length may be a key factor in library production efficiency. Conclusions These results provide a roadmap for labs on the strengths of each of these methods and how best to utilize them. Keywords: RNAseqr; RNA depletion; Illumina; NGS; ABRF; TranscriptomicsNational Cancer Institute (U.S.) (Grant P30-CA14051)National Institute of Environmental Health Sciences (Grant P30-ES002109

    Placement and orientation of individual DNA shapes on lithographically patterned surfaces

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    Artificial DNA nanostructures show promise for the organization of functional materials to create nanoelectronic or nano-optical devices. DNA origami, in which a long single strand of DNA is folded into a shape using shorter 'staple strands', can display 6-nm-resolution patterns of binding sites, in principle allowing complex arrangements of carbon nanotubes, silicon nanowires, or quantum dots. However, DNA origami are synthesized in solution and uncontrolled deposition results in random arrangements; this makes it difficult to measure the properties of attached nanodevices or to integrate them with conventionally fabricated microcircuitry. Here we describe the use of electron-beam lithography and dry oxidative etching to create DNA origami-shaped binding sites on technologically useful materials, such as SiO_2 and diamond-like carbon. In buffer with ~ 100 mM MgCl_2, DNA origami bind with high selectivity and good orientation: 70–95% of sites have individual origami aligned with an angular dispersion (±1 s.d.) as low as ±10° (on diamond-like carbon) or ±20° (on SiO_2)
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