Forcing mechanisms behind variations in TOC concentration of lake waters Forcing mechanisms behind variations in total organic carbon (TOC) concentration of lake waters during the past eight centuries – palaeolimnological evidence from southern Sweden Forcing mechanisms behind variations in TOC concentration of lake waters

International audienceDecadal-scale variations in total organic carbon (TOC) concentration in lake water since AD 1200 in two small lakes in southern Sweden were reconstructed based on visible-near infrared spectroscopy (VNIRS) of their recent sediment successions. In order to assess the impacts of local land-use changes and regional variations in 5 sulphur deposition and climate on the inferred changes in TOC concentration, the same sediment records were subjected to multi-proxy palaeolimnological analyses. Changes in lake-water pH were inferred from diatom analysis, whereas pollen-based land-use reconstructions (Landscape Reconstruction Algorithm) together with geo-chemical records provided information on catchment-scale environmental changes, 10 and comparisons were made with available records of climate and population density. Our long-term reconstructions reveal that TOC concentrations were generally high prior to AD 1900, with second-order variations coupled mainly to changes in agricultural land-use intensity. The last century showed significant changes, and unusually low TOC concentrations were recorded in 1930–1990, followed by a recent increase. Vari-15 ations in sulphur emissions, with an increase in the early 1900s to a peak around AD 1980 and a subsequent decrease, were most likely the main driver of these dynamics, although processes related to the introduction of modern forestry and recent increases in precipitation and temperature may have contributed. The increase in lake-water TOC concentration from around AD 1980 may therefore reflect a recovery process. Given 20 that the effects of sulphate deposition now subside, other forcing mechanisms related to land management and climate change will possibly become the main drivers of TOC concentration changes in boreal lake waters in the future

Historical TOC concentration minima during peak sulfur deposition in two Swedish lakes

International audienceDecadal-scale variations in total organic carbon (TOC) concentration in lake water since AD 1200 in two small lakes in southern Sweden were reconstructed based on visible–near-infrared spectroscopy (VNIRS) of their recent sediment successions. In order to assess the impacts of local land-use changes, regional variations in sulfur, and nitrogen deposition and climate variations on the inferred changes in TOC concentration, the same sediment records were subjected to multi-proxy palaeolimnological analyses. Changes in lake-water pH were inferred from diatom analysis , whereas pollen-based land-use reconstructions (Landscape Reconstruction Algorithm) together with geochemical records provided information on catchment-scale environmental changes, and comparisons were made with available records of climate and population density. Our long-term reconstructions reveal that inferred lake-water TOC concentrations were generally high prior to AD 1900, with additional variability coupled mainly to changes in forest cover and agricultural land-use intensity. The last century showed significant changes, and unusually low TOC concentrations were inferred at AD 1930–1990, followed by a recent increase , largely consistent with monitoring data. Variations in sulfur emissions, with an increase in the early 1900s to a peak around AD 1980 and a subsequent decrease, were identified as an important driver of these dynamics at both sites, while processes related to the introduction of modern forestry and recent increases in precipitation and temperature may have contributed, but the effects differed between the sites. The increase in lake-water TOC concentration from around AD 1980 may therefore reflect a recovery process. Given that the effects of sulfur deposition now subside and that the recovery of lake-water TOC concentrations has reached pre-industrial levels, other forcing mechanisms related to land management and climate change may become the main drivers of TOC concentration changes in boreal lake waters in the future

Two hundred years of land-use change in the South Swedish Uplands : comparison of historical map-based estimates with a pollen-based reconstruction using the landscape reconstruction algorithm

International audienceLong-term records of environmental history at decadal to millennial timescales enable an assessment of ecosystem variability and responses to past anthropogenic disturbances and are fundamental for the development of environmental management strategies. This study examines the local variability of land-use history in the South Swedish Uplands over the last 200 years based on pollen records from three lake-sediment successions. Temporal changes in the proportional cover of 14 plant taxa were quantified as percentages using the landscape reconstruction algorithm (LRA). The LRA-based estimates of the extent of four land-use categories (cropland, meadows/grassland, wetland, outland/woodland) were compared to corresponding estimates based on historical maps and aerial photographs from AD 1769–1823, 1837–1895, 1946 and 2005. Although the LRA approach tends to overestimate grassland cover by 10–30 % for the two earliest time periods, the reconstructed vegetation composition is generally in good agreement with estimates based on the historical records. Subsequently, the LRA approach was used to reconstruct the 200-year history of local land-use dynamics at 20-year intervals around two small lakes. The qualitative assessment of difference approach , which requires fewer assumptions and parameters than LRA for objective evaluation of between-site differences in plant abundances, provides consistent results in general. Significant differences exist in the land-use history between the sites. Local catchment characteristics, such as soil conditions and wetland cover, appear important for the development of human impact on the landscape. Quantifi-cations of past vegetation dynamics provide information on the amplitude, frequency and duration of the land-use changes and their effects on terrestrial and aquatic ecosystems , and should be taken into account when nature conservation strategies are developed

Modern and fossil non-pollen palynomorphs from the Basque mountains (western Pyrenees, France): the use of coprophilous fungi to reconstruct pastoral activity

International audienceThis paper presents results from a modern dataset of non-pollen palynomorphs and its application to aca. 2,000 year peat record from the same area in the western Pyrenees (Basque Country, France). The modern dataset is composed of 35 surface samples (moss polsters) from a mountainous pasture-woodland landscape. Airborne fungal spores (ascospores and conidia), found dominant in the dataset, are linked to the degree of landscape openness and grazing pressure. The complete spectrum of 13 selected spore-types of dung-related Ascomycetes is positively linked with grazing pressure. However, different dung affinities between the spore-types have been identified. These are types clearly related to high grazing pressure and types with no or unclear dung indicative value. The modern dataset is used to aid interpretation of the local fossil pollen record as an independent 'proxy' to assess past pastoral dynamics. This study confirms the utility of modern nonpollen palynomorphs from terrestrial ecosystems in the reconstruction of historical local pastoral activities but also shows their limitation. It may be necessary to extend such study to wetland ecosystems and to investigate the spatial dimension of some fungal spores

Recent climatic and anthropogenic imprints on lacustrine systems in the Pyrenean Mountains inferred from minerogenic and organic clastic supply (Vicdessos valley, Pyrenees, France)

High-resolution seismic profiling has been combined with geochemical analyses of both watershed samples and five lacustrine cores retrieved from two natural lacustrine basins of glacial origin: Lake Majeur and Lake Sigriou (1630 m a.s.l. and 1995 m a.s.l., respectively, Eastern French Pyrenees). Identifying specific minerogenic and organic markers of autochthonous and allochthonous supply, data allow documenting past climatic and anthropogenic pressures. Over the past century, the lacustrine sediment of Lake Majeur has been essentially composed of algae, drastically contrasting with the natural sedimentary infill of the basin, mainly resulting from soil erosion from the mid–late Holocene. Since ad 1907, the Lake Majeur has been used for hydroelectricity production. Human-induced lake-level regulations, affecting up to 37% of the lacustrine surface, have increased by fourfold the accumulation rate of the lake and favoured water enrichment. Rubidium abundance within the lacustrine sediments of the two lakes reflects the mid–late Holocene palaeohydrology. After dam construction in ad 1907, greater quantities of rubidium found in Lake Majeur sedimentary infills indicate drier climatic periods, such as from ad 1975 to ad 1982, during which water reservoirs were particularly in demand. Inversely, before the dam was built, rubidium fluctuations were correlated with wetter conditions and hydrological events were recorded as sandy layers deposited by canyon reactivation, synchronous with European climatic deterioration phases. We notably document that the Mediaeval Climate Anomaly was interrupted by some humid periods dated c. ad 940, ad 1080, ad 1100 and ad 1250. We also date the onset of the ‘Little Ice Age’ c. ad 1360 and identify that this period was wetter after c. ad 1500

Multidisciplinary approach to reconstructing local pastoral activities: an example from the Pyrenean Mountains (Pays Basque)

International audienceIn this study archaeology, history and palaeoecology (modern and fossil data sets of pollen and nonpollen palynomorphs) were used to reconstruct small-scale pastoral activities in the Pyrenees Mountains during the last two millennia. Modern pollen assemblages from the major vegetation units (both natural andanthropogenic) are studied on one restricted watershed area. A correlative model (RDA) of 61 modern pollen spectra and 35 external variables distinguishes two groups of taxa, providing information on the nature and spatial extent of human impact on the landscape. The first pool indicates local pastoral activities, and the second one implies regional input from outside the studied watershed, and is not characteristic of a specific land use. These pools are described as 'Local Pastoral Pollen Indicators' (LPPI) for this particular mountain region on crystalline bedrock and 'Regional Human Activities Pollen Indicators' (RHAPI). The modern data set is used to aid interpretation of the local pollen sequence of Sourzay that covers the last 2000 calendar years BP, using RDA reconstructions, and best modern analogues as a means of comparing modern and fossil spectra. The study also demonstrates agreement between the independent interpretations of two fossil proxies, LPPI and coprophilous fungi

Travelers With Cutaneous Leishmaniasis Cured Without Systemic Therapy

Guidelines recommend wound care and/or local therapy as first-line treatment for cutaneous leishmaniasis. An analysis of a referral treatment program in 135 travelers showed that this approach was feasible in 62% of patients, with positive outcome in 83% of evaluable patient

Europe's lost forests: a pollen-based synthesis for the last 11,000 years

8000 years ago, prior to Neolithic agriculture, Europe was mostly a wooded continent. Since then, its forest cover has been progressively fragmented, so that today it covers less than half of Europe’s land area, in many cases having been cleared to make way for fields and pasture-land. Establishing the origin of Europe’s current, more open land-cover mosaic requires a long-term perspective, for which pollen analysis offers a key tool. In this study we utilise and compare three numerical approaches to transforming pollen data into past forest cover, drawing on >1000 14C-dated site records. All reconstructions highlight the different histories of the mixed temperate and the northern boreal forests, with the former declining progressively since ~6000 years ago, linked to forest clearance for agriculture in later prehistory (especially in northwest Europe) and early historic times (e.g. in north central Europe). In contrast, extensive human impact on the needle-leaf forests of northern Europe only becomes detectable in the last two millennia and has left a larger area of forest in place. Forest loss has been a dominant feature of Europe’s landscape ecology in the second half of the current interglacial, with consequences for carbon cycling, ecosystem functioning and biodiversity

Long-term land-cover/use change in a traditional farming landscape in Romania inferred from pollen data, historical maps and satellite images

Traditional farming landscapes in the temperate zone that have persisted for millennia can be exceptionally species-rich and are therefore key conservation targets. In contrast to Europe’s West, Eastern Europe harbours widespread traditional farming landscapes, but drastic socio-economic and political changes in the twentieth century are likely to have impacted these landscapes profoundly. We reconstructed long-term land-use/cover and biodiversity changes over the last 150 years in a traditional farming landscape of outstanding species diversity in Transylvania. We used the Regional Estimates of Vegetation Abundance from Large Sites model applied to a pollen record from the Transylvanian Plain and a suite of historical and satellite-based maps. We documented widespread changes in the extent and location of grassland and cropland, a loss of wood pastures as well as a gradual increase in forest extent. Land management in the socialist period (1947–1989) led to grassland expansion, but grassland diversity decreased due to intensive production. Land-use intensity has declined since the collapse of socialism in 1989, resulting in widespread cropland abandonment and conversion to grassland. However, these trends may be temporary due to both ongoing woody encroachment as well as grassland management intensification in productive areas. Remarkably, only 8% of all grasslands existed throughout the entire time period (1860–2010), highlighting the importance of land-use history when identifying target areas for conservation, given that old-growth grasslands are most valuable in terms of biodiversity. Combining datasets from different disciplines can yield important additional insights into dynamic landscape and biodiversity changes, informing conservation actions to maintain these species-rich landscapes in the longer term

Towards an In Vitro Model of Plasmodium Hypnozoites Suitable for Drug Discovery

Contains fulltext : 96475.pdf (publisher's version ) (Open Access)BACKGROUND: Amongst the Plasmodium species in humans, only P. vivax and P. ovale produce latent hepatic stages called hypnozoites, which are responsible for malaria episodes long after a mosquito bite. Relapses contribute to increased morbidity, and complicate malaria elimination programs. A single drug effective against hypnozoites, primaquine, is available, but its deployment is curtailed by its haemolytic potential in glucose-6-phosphate dehydrogenase deficient persons. Novel compounds are thus urgently needed to replace primaquine. Discovery of compounds active against hypnozoites is restricted to the in vivo P. cynomolgi-rhesus monkey model. Slow growing hepatic parasites reminiscent of hypnozoites had been noted in cultured P. vivax-infected hepatoma cells, but similar forms are also observed in vitro by other species including P. falciparum that do not produce hypnozoites. METHODOLOGY: P. falciparum or P. cynomolgi sporozoites were used to infect human or Macaca fascicularis primary hepatocytes, respectively. The susceptibility of the slow and normally growing hepatic forms obtained in vitro to three antimalarial drugs, one active against hepatic forms including hypnozoites and two only against the growing forms, was measured. RESULTS: The non-dividing slow growing P. cynomolgi hepatic forms, observed in vitro in primary hepatocytes from the natural host Macaca fascicularis, can be distinguished from similar forms seen in P. falciparum-infected human primary hepatocytes by the differential action of selected anti-malarial drugs. Whereas atovaquone and pyrimethamine are active on all the dividing hepatic forms observed, the P. cynomolgi slow growing forms are highly resistant to treatment by these drugs, but remain susceptible to primaquine. CONCLUSION: Resistance of the non-dividing P. cynomolgi forms to atovaquone and pyrimethamine, which do not prevent relapses, strongly suggests that these slow growing forms are hypnozoites. This represents a first step towards the development of a practical medium-throughput in vitro screening assay for novel hypnozoiticidal drugs