ORAL LESIONS OF HIV-INFECTED CHILDREN IN WEST AFRICA IN THE ERA OF ANTIRETROVIRAL TREATMENTS

Oral Communication presented at the "Forum des Jeunes Chercheurs", Brest (France) 2011

HIV Status Disclosure and Retention in Care in HIV-Infected Adolescents on Antiretroviral Therapy (ART) in West Africa

We assessed the effect of HIV status disclosure on retention in care from initiation of antiretroviral therapy (ART) among HIV-infected children aged 10 years or more in Cote d'Ivoire, Mali and Sénégal.Multi-centre cohort study within five paediatric clinics participating in the IeDEA West Africa collaboration. HIV-infected patients were included in this study if they met the following inclusion criteria: aged 10-21 years while on ART; having initiated ART ≥ 200 days before the closure date of the clinic database; followed ≥ 15 days from ART initiation in clinics with ≥ 10 adolescents enrolled. Routine follow-up data were merged with those collected through a standardized ad hoc questionnaire on awareness of HIV status. Probability of retention (no death or loss-to-follow-up) was estimated with Kaplan-Meier method. Cox proportional hazard model with date of ART initiation as origin and a delayed entry at date of 10th birthday was used to identify factors associated with death or loss-to-follow-up.650 adolescents were available for this analysis. Characteristics at ART initiation were: median age of 10.4 years; median CD4 count of 224 cells/mm³ (47% with severe immunosuppression), 48% CDC stage C/WHO stage 3/4. The median follow-up on ART after the age of 10 was 23.3 months; 187 adolescents (28.8%) knew their HIV status. The overall probability of retention at 36 months after ART initiation was 74.6% (95% confidence interval [CI]: 70.5-79.0) and was higher for those disclosed compared to those not: adjusted hazard ratio for the risk of being death or loss-to-follow-up = 0.23 (95% CI: 0.13-0.39).About 2/3 of HIV-infected adolescents on ART were not aware of their HIV status in these ART clinics in West Africa but disclosed HIV status improved retention in care. The disclosure process should be thus systematically encouraged and organized in adolescent populations

The efficacy of R-Endo rotary NiTi and stainless-steel hand instruments to remove gutta-percha and Resilon.

International audienceAIM: To evaluate the efficacy of the R-Endo rotary nickel titanium instrumentation system and hand instrumentation to remove gutta-percha or Resilon from root canals. METHODOLOGY: Eighty single-rooted teeth with straight canals were selected, the pulp chamber was opened, and the canals prepared to a size 30, 0.04 taper. The teeth were divided randomly into two groups of 40 each for root filling with gutta-percha + MMSeal sealer or Resilon + RealSeal sealer. After storage, each group was divided into two subgroups, and material was removed with Hedström files or with R-Endo instrumentation. Times to reach the working length and to eliminate filling material were recorded. Root canal walls were examined using scanning electron microscope to evaluate material remnants on each third. The presence of material was measured according to a score scale. The Student's t-test was used to determine significant differences between mean values of time and remaining material (P 0.05). However, times to reach the working length and for removal of filling were lower with R-Endo than with Hedström files (P < 0.0001). CONCLUSIONS: Both instrumentation techniques left filling material inside the root canal and mainly in the apical third. There was no difference between the instrumentation techniques

Evaluation of rapid assays for screening and confirming HIV-1 infection in Ethiopia.

To evaluate a simple and rapid testing strategy to diagnose HIV infection in Ethiopia, we subjected a panel of 688 sera with known HIV serologic status (confirmed by ELISA/WB or double ELISA) to 3 rapid assays: Determine HIV-1/2, Capillus HIV-1/2 and Serocard HIV. Samples were obtained from participants in a cohort study on HIV-infection (72%), from tuberculosis patients (18%) and from participants in surveillance studies among police recruits and commercial sex workers (10%). The panel consisted of 249 HIV-1 positive samples, of which 68 were HIV-1 subtype C and 1 HIV-1 subtype A, and 439 HIV-1 negative samples. Determine and Capillus were 100% sensitive and 99.8% specific, Serocard was 100% sensitive and specific. On retrospective evaluation, both parallel (samples tested simultaneously by two rapid assays) and serial (samples tested by two consecutive rapid assays) testing algorithms were 100% sensitive and specific when compared to ELISA/WB or double ELISA testing strategy. In conclusion rapid assays have high sensitivity and specificity. HIV serodiagnosis based on rapid assays may therefore be a valuable alternative in voluntary counselling and testing centres and in facilities where sophisticated laboratories are not available

Performance of routine syphilis serology in the Ethiopian cohort on HIV/AIDS

OBJECTIVES: To assess the performance of routine syphilis screening during 5 year follow up of Ethiopian factory workers, participating in a cohort study on HIV/AIDS. METHODS: Syphilis serology test results of factory workers, who each donated at least six blood samples were evaluated. Screening in 1997-8 had been performed by the Treponema pallidum particle agglutination (TPPA) assay and in 1999-2001 by the rapid plasma reagin (RPR) test. TPPA had been followed by RPR or RPR by TPPA, in case of a positive screening result. Samples of study subjects showing inconsistent sequential TPPA and/or RPR results were retested independently by three laboratory technicians. RESULTS: A total of 540 cohort participants (8.3% HIV positive at enrollment) donated 4,376 blood samples (mean 8.3 per subject). From 93 of the 176 participants with at least one positive TPPA result during follow up, 152 samples were retested by RPR and/or TPPA. Based on the revised syphilis test results, the 540 cohort participants were classified as having no (70.5%), past (20.6%), prevalent (6.9%), or incident (2.0%) syphilis. The RPR screening test was difficult to interpret and yielded 8.2% biological false positive (BFP) RPR results, or 3.2% if weak positive results were excluded. There was no correlation between HIV infection and BFP RPR reactions. Sample mix-ups were detected in 1.2%. CONCLUSION: Evaluation of routine syphilis screening as performed in a long term cohort study on HIV/AIDS in Ethiopia showed difficulties encountered in syphilis screening programmes such as a high percentage of BFP RPR, inconsistencies in interpretation of the RPR test, and sample mix ups. The findings stress the need to develop a syphilis screening assay that is easy to perform and interpret and to implement quality assurance programme

Critères de repérage précoce des enfants à risque de développer un surpoids

info:eu-repo/semantics/publishe

Evaluation of the oral health of pregnant women in prenatal consultations in Côte d’Ivoire

Abstract Background Despite the proven links between oral health status and adverse pregnancy outcomes, oral examination is not systematically integrated into prenatal examinations in Côte d’Ivoire. In addition, there are no prevention messages specifically targeted at pregnant women. Objective: To assess the oral health of pregnant women in Côte d’Ivoire. Methods A cross-sectional survey was conducted at the Maternal and Child Health Department of the National Public Health Institute in Abidjan. The data collected were: age, education level, marital status, source of income, trimester of pregnancy, gestational age, oral hygiene habits and the fact that they whether or not received oral health information. A clinical examination was conducted to assess oral hygiene (OHIS index), periodontal condition (CPI index), dental condition (DMFT index), as well as the presence of epulis, erosion or dental mobility. Frequencies and counts were calculated for the different variables in the study. Results A total of 207 women were observed. The age range was 15 to 44 years with 14.0% under 19 years old. The majority were in couple (76.8%), multi-gesture (77.3%), and more than half had no education (52.2%) or source of income (56.0%); 50.7% snacked between meals and 33.3% had vomiting. They brushed their teeth at least twice a day (70.0%) and had not changed their oral hygiene habits (77.3%) during this pregnancy. Only 3.4% had information on oral health conditions during pregnancy. The frequency of caries was 75.4%, dental erosions 13.0%, gingivitis 57.0%, periodontitis 6.8% and epulis 3.4%. Conclusions The results suggest the integration of systematic dental consultation into prenatal consultations in order permit the sensitization of these women, screening and management of their oral conditions. Key messages Oral diseases of pregnant women are a reality without their knowledge in Côte d’Ivoire. A systematic dental visit is essential during prenatal consultations in Côte d’Ivoire. </jats:sec