A comparison of head and manual control for a position-control pursuit tracking task

Head control was compared with manual control in a pursuit tracking task involving proportional controlled-element dynamics. An integrated control/display system was used to explore tracking effectiveness in horizontal and vertical axes tracked singly and concurrently. Compared with manual tracking, head tracking resulted in a 50 percent greater rms error score, lower pilot gain, greater high-frequency phase lag and greater low-frequency remnant. These differences were statistically significant, but differences between horizontal- and vertical-axis tracking and between 1- and 2-axis tracking were generally small and not highly significant. Manual tracking results were matched with the optimal control model using pilot-related parameters typical of those found in previous manual control studies. Head tracking performance was predicted with good accuracy using the manual tracking model plus a model for head/neck response dynamics obtained from the literature

Progress Towards Modeling the Ablation Response of NuSil-Coated PICA

The Mars Science Laboratory (MSL) Entry, Descent and Landing Instrumentation (MEDLI) collected in-flight data largely used by the ablation community to verify and validate physics-based models for the response of the Phenolic Impregnated Carbon Ablator (PICA) material [1-4]. MEDLI data were recently used to guide the development of NASAs high-fidelity material response models for PICA, implemented in the Porous material Analysis Toolbox based on OpenFOAM (PATO) software [5-6]. A follow-up instrumentation suite, MEDLI2, is planned for the upcoming Mars 2020 mission [7] after the large scientific impact of MEDLI. Recent analyses performed as part of MEDLI2 development draw the attention to significant effects of a protective coating to the aerothermal response of PICA. NuSil, a silicone-based overcoat sprayed onto the MSL heatshield as contamination control, is currently neglected in PICA ablation models. To mitigate the spread of phenolic dust from PICA, NuSil was applied to the entire MSL heatshield, including the MEDLI plugs. NuSil is a space grade designation of the siloxane copolymer, primarily used to protect against atomic oxygen erosion in the Low Earth Orbit environment. Ground testing of PICA-NuSil (PICA-N) models all exhibited surface temperature jumps of the order of 200 K due to oxide scale formation and subsequent NuSil burn-off. It is therefore critical to include a model for the aerothermal response of the coating in ongoing code development and validation efforts

Two dimensional eye tracking: Sampling rate of forcing function

A study was conducted to determine the minimum update rate of a forcing function display required for the operator to approximate the tracking performance obtained on a continuous display. In this study, frequency analysis was used to determine whether there was an associated change in the transfer function characteristics of the operator. It was expected that as the forcing function display update rate was reduced, from 120 to 15 samples per second, the operator's response to the high frequency components of the forcing function would show a decrease in gain, an increase in phase lag, and a decrease in coherence

Head tracking at large angles from the straight ahead position

One of the big advantages of a helmet sight in a high performance aircraft is its off-boresight capability in aiming a fire control system. However, tracking data using a target that is moving rapidly and randomly for an extended period of time is missing. This study is intended to provide data in this area that will be of value to engineers in designing head control systems

Whole genome sequencing-based mapping and candidate identification of mutations from fixed zebrafish tissue

As forward genetic screens in zebrafish become more common, the number of mutants that cannot be identified by gross morphology or through transgenic approaches, such as many nervous system defects, has also increased. Screening for these difficult-to-visualize phenotypes demands techniques such as whole-mount in situ hybridization (WISH) or antibody staining, which require tissue fixation. To date, fixed tissue has not been amenable for generating libraries for whole genome sequencing (WGS). Here, we describe a method for using genomic DNA from fixed tissue and a bioinformatics suite for WGS-based mapping of zebrafish mutants. We tested our protocol using two known zebrafish mutant alleles, gpr126st49 and egr2bfh227, both of which cause myelin defects. As further proof of concept we mapped a novel mutation, stl64, identified in a zebrafish WISH screen for myelination defects. We linked stl64 to chromosome 1 and identified a candidate nonsense mutation in the F-box and WD repeat domain containing 7 (fbxw7) gene. Importantly, stl64 mutants phenocopy previously described fbxw7vu56 mutants, and knockdown of fbxw7 in wild-type animals produced similar defects, demonstrating that stl64 disrupts fbxw7. Together, these data show that our mapping protocol can map and identify causative lesions in mutant screens that require tissue fixation for phenotypic analysis

Human Amniocytes Are Receptive to Chemically Induced Reprogramming to Pluripotency

Restoring pluripotency using chemical compounds alone would be a major step forward in developing clinical-grade pluripotent stem cells, but this has not yet been reported in human cells. We previously demonstrated that VPA_ AFS cells, human amniocytes cultivated with valproic acid (VPA) acquired functional pluripotency while remaining distinct from human embryonic stem cells (hESCs), questioning the relationship between the modulation of cell fate and molecular regulation of the pluripotency network. Here, we used single-cell analysis and functional assays to reveal that VPA treatment resulted in a homogeneous population of self-renewing non-transformed cells that fulfill the hallmarks of pluripotency, i.e., a short G1 phase, a dependence on glycolytic metabolism, expression of epigenetic modifications on histones 3 and 4, and reactivation of endogenous OCT4 and downstream targets at a lower level than that observed in hESCs. Mechanistic insights into the process of VPA-induced reprogramming revealed that it was dependent on OCT4 promoter activation, which was achieved independently of the PI3K (phosphatidylinositol 3-kinase)/ AKT/ mTOR (mammalian target of rapamycin) pathway or GSK3 beta inhibition but was concomitant with the presence of acetylated histones H3K9 and H3K56, which promote pluripotency. Our data identify, for the first time, the pluripotent transcriptional and molecular signature and metabolic status of human chemically induced pluripotent stem cells

A description of n-ary semigroups polynomial-derived from integral domains

We provide a complete classification of the n-ary semigroup structures defined by polynomial functions over infinite commutative integral domains with identity, thus generalizing G{\l}azek and Gleichgewicht's classification of the corresponding ternary semigroups

Lyman-alpha absorption around nearby galaxies

We have used STIS aboard HST to search for Lyman-alpha (Lya) absorption lines in the outer regions of eight nearby galaxies using background QSOs and AGN as probes. Lya lines are detected within a few hundred km/s of the systemic velocity of the galaxy in all cases. We conclude that a background line-of-sight which passes within 26-200 h-1 kpc of a foreground galaxy is likely to intercept low column density neutral hydrogen with log N(HI) >~ 13.0. The ubiquity of detections implies a covering factor of ~ 100% for low N(HI) gas around galaxies within 200 h-1 kpc. We discuss the difficulty in trying to associate individual absorption components with the selected galaxies and their neighbors, but show that by degrading our STIS data to lower resolutions, we are able to reproduce the anti-correlation of Lya equivalent width and impact parameter found at higher redshift. We also show that the equivalent width and column density of Lya complexes (when individual components are summed over ~ 1000 km/s) correlate well with a simple estimate of the volume density of galaxies brighter than M(B) = -17.5 at the same redshift as a Lya complex. We do not reject the hypothesis that the selected galaxies are directly responsible for the observed Lya lines, but our analysis indicates that absorption by clumpy intragroup gas is an equally likely explanation. (Abriged)Comment: Accepted for publication in Nov 20, 2002 issue of ApJ. Paper with all figures can be found at http://www.astro.princeton.edu/~dvb/lyapaper.ps (preferable). Minor typos fixe

How are normal sleeping controls selected? A systematic review of cross-sectional insomnia studies, and a standardised method to select healthy controls for sleep research

There appears to be some inconsistency in how normal sleepers (controls) are selected and screened for participation in research studies for comparison with insomnia patients. The purpose of the current study is to assess and compare methods of identifying normal sleepers in insomnia studies, with reference to published standards. We systematically reviewed the literature on insomnia patients which included control subjects. The resulting 37 articles were systematically reviewed with reference to the five criteria for normal sleep specified by Edinger et al. (2004). In summary, these criteria are: evidence of sleep disruption; sleep scheduling; general health; substance/medication use; and other sleep disorders. We found sleep diaries, PSG, and clinical screening examinations to be widely used with both control subjects and insomnia participants. However, there are differences between research groups in the precise definitions applied to the components of normal sleep. We found that none of reviewed studies applied all of the Edinger et al. criteria, and 16% met four criteria. In general, screening is applied most rigorously at the level of a clinical disorder, whether physical, psychiatric, or sleep. While the Edinger et al. criteria seem to be applied in some form by most researchers, there is scope to improve standards and definitions in this area. Ideally, different methods such as sleep diaries and questionnaires would be used concurrently with objective measures to ensure normal sleepers are identified, and descriptive information for control subjects would be reported. Here, we have devised working criteria and methods to be used for assessment of normal sleepers. This would help clarify the nature of the control group, in contrast to insomnia subjects and other patient groups