1,638 research outputs found

    Variable-Number-of-Tandem-Repeats Analysis of Genetic Diversity in Pasteuria ramosa

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    Variable-number-of-tandem-repeats (VNTR) markers are increasingly being used in population genetic studies of bacteria. They were recently developed for Pasteuria ramosa, an endobacterium that infects Daphnia species. In the present study, we genotyped P. ramosa in 18 infected hosts from the United Kingdom, Belgium, and two lakes in the United States using seven VNTR markers. Two Daphnia species were collected: D. magna and D. dentifera. Six loci showed length polymorphism, with as many as five alleles identified for a single locus. Similarity coefficient calculations showed that the extent of genetic variation between pairs of isolates within populations differed according to the population, but it was always less than the genetic distances among populations. Analysis of the genetic distances performed using principal component analysis revealed strong clustering by location of origin, but not by host Daphnia species. Our study demonstrated that the VNTR markers available for P. ramosa are informative in revealing genetic differences within and among populations and may therefore become an important tool for providing detailed analysis of population genetics and epidemiolog

    3D Particle Tracking Velocimetry Method: Advances and Error Analysis

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    A full three-dimensional particle tracking system was developed and tested. By using three separate CCDs placed at the vertices of an equilateral triangle, the threedimensional location of particles can be determined. Particle locations measured at two different times can then be used to create a three-component, three-dimensional velocity field. Key developments are: the ability to accurately process overlapping particle images, offset CCDs to significantly improve effective resolution, allowance for dim particle images, and a hybrid particle tracking technique ideal for three-dimensional flows when only two sets of images exist. An in-depth theoretical error analysis was performed which gives the important sources of error and their effect on the overall system. This error analysis was verified through a series of experiments, which utilized a test target with 100 small dots per square inch. For displacements of 2.54mm the mean errors were less than 2% and the 90% confidence limits were less than 5.2 μm in the plane perpendicular to the camera axis, and 66 μm in the direction of the camera axis. The system was used for flow measurements around a delta wing at an angle of attack. These measurements show the successful implementation of the system for three-dimensional flow velocimetry

    On imploding cylindrical and spherical shock waves in a perfect gas

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    The problem of a cylindrically or spherically imploding and reflecting shock wave in a flow initially at rest is studied without the use of the strong-shock approximation. Dimensional arguments are first used to show that this flow admits a general solution where an infinitesimally weak shock from infinity strengthens as it converges towards the origin. For a perfect-gas equation of state, this solution depends only on the dimensionality of the flow and on the ratio of specific heats. The Guderley power-law result can then be interpreted as the leading-order, strong-shock approximation, valid near the origin at the implosion centre. We improve the Guderley solution by adding two further terms in the series expansion solution for both the incoming and the reflected shock waves. A series expansion, valid where the shock is still weak and very far from the origin, is also constructed. With an appropriate change of variables and using the exact shock-jump conditions, a numerical, characteristics-based solution is obtained describing the general shock motion from almost infinity to very close to the reflection point. Comparisons are made between the series expansions, the characteristics solution, and the results obtained using an Euler solver. These show that the addition of two terms to the Guderley solution significantly extends the range of validity of the strong-shock series expansion

    MUN Festival and Chamber Choirs, Douglas Dunsmore, conductor (April 7, 1991)

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    MUN Festival and Chamber Choirs, Douglas Dunsmore, conductor (April 7, 1991

    The 2mrad horizontal crossing angle IR layout for a TeV ILC

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    The current status of the 2mrad crossing angle layout for the ILC is reviewed. The scheme developed in the UK and France is described and the performance discussed for a TeV machine. Secondly, the scheme developed at SLAC and BNL is then studied and modified for a TeV machine. We find that both schemes can handle the higher energy beam with modifications, and share many common features.Comment: The proceedings of the 2005 International Linear Collider Workshop, March 2005. 4 pages, 5 figure

    An Injector for the CLIC Test Facility (CTF3)

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    The CLIC Test Facility (CTF3) is an intermediate step to demonstrate the technical feasibility of the key concepts of the new RF power source for CLIC. CTF3 will use electron beams with an energy range adjustable from 170 MeV (3.5 A) to 380 MeV (with low current). The injector is based on a thermionic gun followed by a classical bunching system embedded in a long solenoidal field. As an alternative, an RF photo-injector is also being studied. The beam dynamics studies on how to reach the stringent beam parameters at the exit of the injector are presented. Simulations performed with the EGUN code showed that a current of 7 A can be obtained with an emittance less than 10 mm.mrad at the gun exit. PARMELA results are presented and compared to the requested beam performance at the injector exit. Sub-Harmonic Bunchers (SHB) are foreseen, to switch the phase of the bunch trains by 180 degrees from even to odd RF buckets. Specific issues of the thermionic gun and of the SHB with fast phase switch are discussed

    Modelling fish habitat preference with a genetic algorithm-optimized Takagi-Sugeno model based on pairwise comparisons

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    Species-environment relationships are used for evaluating the current status of target species and the potential impact of natural or anthropogenic changes of their habitat. Recent researches reported that the results are strongly affected by the quality of a data set used. The present study attempted to apply pairwise comparisons to modelling fish habitat preference with Takagi-Sugeno-type fuzzy habitat preference models (FHPMs) optimized by a genetic algorithm (GA). The model was compared with the result obtained from the FHPM optimized based on mean squared error (MSE). Three independent data sets were used for training and testing of these models. The FHPMs based on pairwise comparison produced variable habitat preference curves from 20 different initial conditions in the GA. This could be partially ascribed to the optimization process and the regulations assigned. This case study demonstrates applicability and limitations of pairwise comparison-based optimization in an FHPM. Future research should focus on a more flexible learning process to make a good use of the advantages of pairwise comparisons
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