Genetic Patterns of Domestication in Pigeonpea (Cajanus cajan (L.) Millsp.) and Wild Cajanus Relatives

Pigeonpea (Cajanus cajan) is an annual or short-lived perennial food legume of acute regional importance, providing significant protein to the human diet in less developed regions of Asia and Africa. Due to its narrow genetic base, pigeonpea improvement is increasingly reliant on introgression of valuable traits from wild forms, a practice that would benefit from knowledge of its domestication history and relationships to wild species. Here we use 752 single nucleotide polymorphisms (SNPs) derived from 670 low copy orthologous genes to clarify the evolutionary history of pigeonpea (79 accessions) and its wild relatives (31 accessions). We identified three well-supported lineages that are geographically clustered and congruent with previous nuclear and plastid sequence-based phylogenies. Among all species analyzed Cajanus cajanifolius is the most probable progenitor of cultivated pigeonpea. Multiple lines of evidence suggest recent gene flow between cultivated and non-cultivated forms, as well as historical gene flow between diverged but sympatric species. Evidence supports that primary domestication occurred in India, with a second and more recent nested population bottleneck focused in tropical regions that is the likely consequence of pigeonpea breeding. We find abundant allelic variation and genetic diversity among the wild relatives, with the exception of wild species from Australia for which we report a third bottleneck unrelated to domestication within India. Domesticated C. cajan possess 75% less allelic diversity than the progenitor clade of wild Indian species, indicating a severe ‘‘domestication bottleneck’’ during pigeonpea domestication

Multiple post-domestication origins of kabuli chickpea through allelic variation in a diversification-associated transcription factor

Chickpea (Cicer arietinum) is among the founder crops domesticated in the Fertile Crescent. One of two major forms of chickpea, the so-called kabuli type, has white flowers and light-colored seed coats, properties not known to exist in the wild progenitor. The origin of the kabuli form has been enigmatic. We genotyped a collection of wild and cultivated chickpea genotypes with 538 single nucleotide polymorphisms (SNPs) and examined patterns of molecular diversity relative to geographical sources and market types. In addition, we examined sequence and expression variation in candidate anthocyanin biosynthetic pathway genes. A reduction in genetic diversity and extensive genetic admixture distinguish cultivated chickpea from its wild progenitor species. Among germplasm, the kabuli form is polyphyletic. We identified a basic helix-loop-helix (bHLH) transcription factor at chickpea\u27s B locus that conditions flower and seed colors, orthologous to Mendel\u27s A gene of garden pea, whose loss of function is associated invariantly with the kabuli type of chickpea. From the polyphyletic distribution of the kabuli form in germplasm, an absence of nested variation within the bHLH gene and invariant association of loss of function of bHLH among the kabuli type, we conclude that the kabuli form arose multiple times during the phase of phenotypic diversification after initial domestication of cultivated chickpea

Post-harvest management and associated food losses and by-products of cassava in southern Ethiopia

Improved (high yield and disease resistant) cassava varieties were introduced into Ethiopia around the onset of the twenty-first century, as a potential food security crop. At present, limited information is available from the country on post-production aspects of the value chain (VC) and related food losses. The lack of such data prevents policymakers and VC actors from taking steps towards improving VC efficiencies, which can have a significant impact on livelihoods and food security. The focus of this study was to examine the prevailing post-harvest practices in the cassava VC in southern Ethiopia and quantify the extent of food losses and associated by-products in the framework of the recently developed ‘food loss and waste protocol’. The majority of the cassava in the study area was processed into dry chips and milled into a composite flour with teff and maize to prepare the staple bread (injera). ‘Critical loss points’ were during sun-drying (4%) and stockpiling at farm and marketplace (30–50%). Insect pest damage was primarily responsible for food losses at farm and market level. The most important insect species infesting dry cassava were identified during the survey. As far as the by-products were concerned, the ratio of leaf:wood (stem and stump):starchy root on a dry matter basis at harvest was 1:6:10. Further emphasis should be on improving processing and storage technologies to reduce food losses and the better recovery and utilisation of by-products, especially the leaves of cassava, which could be a potential source of protein in human diets

Proceeding Report of the 40th Anniversary of National Meteorological Agency, Official endorsement of the National Framework for Climate Services an International Scientific Conference

Ethiopia is located in the Horn of Africa within 3–15° N and 33–48° E, bordered by Eritrea to the north and northeast, Djibouti to the east, Sudan to the west, Kenya to the south, and Somalia to the south and east. It covers an area of about 1.14 million square kilometers. The country's topography consists of high and rugged plateaus and peripheral lowlands. Elevations in the country range from 160 meters below sea level (northern exit of the Rift Valley) to over 4600 meters above sea level (of northern mountainous regions). The highest mountains are concentrated on the northern and southern plateaus of the country. A large percentage of the country consists of high plateaus and mountain ranges, dissected by major rivers such as Blue Nile, Tekeze, Awash, Omo, Wabi Shebelle, etc. Overall, Ethiopia consists of 9 major rivers and 19 lakes. The Blue Nile, the chief headstream of the Nile, rises in Lake Tana in northwest Ethiopia. The meteorological observation started in the 1890s with few meteorological stations. In 1951, meteorological services were established as a small unit in the then Civil Aviation Department to render aeronautical services. Since then, meteorological observation has been expanding over Ethiopia. As the importance of meteorology was realized by other economic sectors, National Meteorological Services Agency (NMSA) was established by the Government Proclamation Number 201/1980. Besides, NMA started seasonal forecasting and advisory service in 1987 for three seasons, namely Belg (February - May), Kiremt (June-September), and Bega (October-January). The primary duty of NMA is to support all country's socio-economic developments by delivering climate services. Moreover, NMA has more than one thousand three hundred conventional Meteorological stations, three hundred automatic weather stations, five AWOS, three air pollution monitoring stations, three upper air stations, one radar, and eleven satellite receiver stations. The government of Ethiopia is determined to eradicate poverty and become a prosperous country by 2030. In this regard, addressing climate variability and change play a pivotal role in achieving this goal. To this effect, NMA is equipping with modern weather observing and monitoring capabilities and improving processing, analyzing, interpreting, and forecasting weather and climate capabilities at a high resolution and accuracy to meet the end-user's demands and effectively support all socio-economic developments of the country. NMA collaborates with all key stakeholders and partners through continuous engagement on climate services. The NFCS, endorsed during the NMA 40th Anniversary, is envisaged to strengthen collaborative co-production between climate services provider (NMA) and climate service beneficiaries institutions (MoWIE, EFCCC, MoA, MoH, and NDRMC). To commemorate its 40th anniversary, NMA has organized a conference with a theme of "Forty Years of Climate and Weather Services in Ethiopia" on May 25-26, 2021, at Skylight Hotel, Addis Ababa, Ethiopia. Generally, three main sessions were held during the conference. These include; Session one: panel discussion and opening ceremony; session two: presentations on the history of NMA and NFCS Ethiopia, official endorsement of NFSC Ethiopia, and certificate wards; and session three: parallel session of four groups and paper presentations on different thematic areas were made. The 40th anniversary was attended by ministers, commissioners, heads of organizations, and representatives from the WMO Africa regional office, international institutes representatives, experts from different organizations, lecturers, researchers, and NMA officials and staff. This document is proceedings of the 40th anniversary, including summaries of the opening session and the presentations

Genetic Patterns of Domestication in Pigeonpea (Cajanus cajan (L.) Millsp.) and Wild Cajanus Relatives

Pigeonpea (Cajanus cajan) is an annual or short-lived perennial food legume of acute regional importance, providing significant protein to the human diet in less developed regions of Asia and Africa. Due to its narrow genetic base, pigeonpea improvement is increasingly reliant on introgression of valuable traits from wild forms, a practice that would benefit from knowledge of its domestication history and relationships to wild species. Here we use 752 single nucleotide polymorphisms (SNPs) derived from 670 low copy orthologous genes to clarify the evolutionary history of pigeonpea (79 accessions) and its wild relatives (31 accessions). We identified three well-supported lineages that are geographically clustered and congruent with previous nuclear and plastid sequence-based phylogenies. Among all species analyzed Cajanus cajanifolius is the most probable progenitor of cultivated pigeonpea. Multiple lines of evidence suggest recent gene flow between cultivated and non-cultivated forms, as well as historical gene flow between diverged but sympatric species. Evidence supports that primary domestication occurred in India, with a second and more recent nested population bottleneck focused in tropical regions that is the likely consequence of pigeonpea breeding. We find abundant allelic variation and genetic diversity among the wild relatives, with the exception of wild species from Australia for which we report a third bottleneck unrelated to domestication within India. Domesticated C. cajan possess 75% less allelic diversity than the progenitor clade of wild Indian species, indicating a severe “domestication bottleneck” during pigeonpea domestication

Global Roadkill Data: a dataset on terrestrial vertebrate mortality caused by collision with vehicles

Roadkill is widely recognized as one of the primary negative effects of roads on many wildlife species and also has socioeconomic impacts when they result in accidents. A comprehensive dataset of roadkill locations is essential to evaluate the factors contributing to roadkill risk and to enhance our comprehension of its impact on wildlife populations and socioeconomic dimensions. We undertook a compilation of roadkill records, encompassing both published and unpublished data gathered from road surveys or opportunistic sources. GLOBAL ROADKILL DATA includes 208,570 roadkill records of terrestrial vertebrates from 54 countries across six continents, encompassing data collected between 1971 and 2024. This dataset serves to minimise the collection of redundant data and acts as a valuable resource for local and macro scale analysis regarding rates of roadkill, road- and landscape-related features associated with risk of roadkill, vulnerability of species to road traffic, and populations at risk of local extinction. The objective of this dataset is to promote scientific progress in infrastructure ecology and terrestrial vertebrate conservation while limiting the socio-economic costs

Prevalence of trachoma at sub-district level in Ethiopia : determining when to stop mass azithromycin distribution

To eliminate blinding trachoma, the World Health Organization emphasizes implementing the SAFE strategy, which includes annual mass drug administration (MDA) with azithromycin to the whole population of endemic districts. Prevalence surveys to assess impact at the district level are recommended after at least 3 years of intervention. The decision to stop MDA is based on a prevalence of trachomatous inflammation follicular (TF) among children aged 1-9 years below 5% at the sub-district level, as determined by an additional round of surveys limited within districts where TF prevalence is below 10%. We conducted impact surveys powered to estimate prevalence simultaneously at the sub-district and district in two zones of Amhara, Ethiopia to determine whether MDA could be stopped.; Seventy-two separate population-based, sub-district surveys were conducted in 25 districts. In each survey all residents from 10 randomly selected clusters were screened for clinical signs of trachoma. Data were weighted according to selection probabilities and adjusted for correlation due to clustering.; Overall, 89,735 residents were registered from 21,327 households of whom 72,452 people (80.7%) were examined. The prevalence of TF in children aged 1-9 years was below 5% in six sub-districts and two districts. Sub-district level prevalence of TF in children aged 1-9 years ranged from 0.9-76.9% and district-level from 0.9-67.0%. In only one district was the prevalence of trichiasis below 0.1%.; The experience from these zones in Ethiopia demonstrates that impact assessments designed to give a prevalence estimate of TF at sub-district level are possible, although the scale of the work was challenging. Given the assessed district-level prevalence of TF, sub-district-level surveys would have been warranted in only five districts. Interpretation was not as simple as stopping MDA in sub-districts below 5% given programmatic challenges of exempting sub-districts from a highly regarded programme and the proximity of hyper-endemic sub-districts

Incidence of cesarean delivery after induction of labor with intravenous oxytocin drip among women undergoing induction of labor at Dessie referral hospital, Northeast Ethiopia, 2017

&lt;p class="abstract"&gt;&lt;strong&gt;Background:&lt;/strong&gt; The number of delivering women undergoing an induction of labor is greater than 20% and continues to rise. Simultaneously, the cesarean delivery rate continues to increase as well. This increase has resulted from evidence-based recommendations on how to handle certain conditions. Labor induction has been associated with increased likelihood of cesarean birth for some groups of women.&lt;/p&gt;&lt;p class="abstract"&gt;&lt;strong&gt;Methods:&lt;/strong&gt; Institutional based retrospective cross sectional study was conducted on 319 women medical chard who undergone induction of labor with oxytocin infusion at Dessie referral hospital. Systematic sampling techniques was used to select the samples. The data was cleaned, edited, coded, and entered in to EPI INFO version 3.5 and exported and analyzed by SPSS with windows version 20.0.  &lt;/p&gt;&lt;p class="abstract"&gt;&lt;strong&gt;Results:&lt;/strong&gt; A total of 319 delivery records were reviewed. Out of this 256 (80.3%) was successful induction of labor. Incidence of cesarean section after induction of labor with oxytocin infusion among women at Dessie referral hospital was 136 (42.6%). The most frequent cause of induction of labor was due to hypertensive disorder 133 (41.7%) followed by pre labor rupture of membrane 111 (34.8%). Cesarean section was done due to failed induction of labor 63 (19.7%) followed by fetal distress 40 (12.5%).&lt;/p&gt;&lt;p class="abstract"&gt;&lt;strong&gt;Conclusions:&lt;/strong&gt; In present study incidence of cesarean delivery after induction of labor was 42.6%. Most frequent cause of induction of labor was due to hypertensive disorder followed by pre-labor rupture of membrane. Cesarean section was done due to failed induction of labor followed by fetal distress.&lt;/p&gt;</jats:p