The Wolf-Rayet binaries of the nitrogen sequence in the Large Magellanic Cloud: spectroscopy, orbital analysis, formation, and evolution

Massive Wolf-Rayet (WR) stars dominate the radiative and mechanical energy budget of galaxies and probe a critical phase in the evolution of massive stars prior to core-collapse. It is not known whether core He-burning WR stars (classical WR, cWR) form predominantly through wind-stripping (w-WR) or binary stripping (b-WR). With spectroscopy of WR binaries so-far largely avoided due to its complexity, our study focuses on the 44 WR binaries / binary candidates of the Large Magellanic Cloud (LMC, metallicity Z~0.5 Zsun), identified on the basis of radial velocity variations, composite spectra, or high X-ray luminosities. Relying on a diverse spectroscopic database, we aim to derive the physical and orbital parameters of our targets, confronting evolution models of evolved massive stars at sub-solar metallicity, and constraining the impact of binary interaction in forming them. Spectroscopy is performed using the Potsdam Wolf-Rayet (PoWR) code and cross-correlation techniques. Disentanglement is performed using the code Spectangular or the shift-and-add algorithm. Evolutionary status is interpreted using the Binary Population and Spectral Synthesis (BPASS) code, exploring binary interaction and chemically-homogeneous evolution. No obvious dichotomy in the locations of apparently-single and binary WN stars on the Hertzsprung-Russell diagram is apparent. According to commonly used stellar evolution models (BPASS, Geneva), most apparently-single WN stars could not have formed as single stars, implying that they were stripped by an undetected companion. Otherwise, it must follow that pre-WR mass-loss/mixing (e.g., during the red supergiant phase) are strongly underestimated in standard stellar evolution models.Comment: accepted to A&A on 10.05.2019; 69 pages (25 main paper + 44 appendix); Corrigendum: Shenar et al. 2020, A&A, 641, 2: An unfortunate typo in the implementation of the "transformed radius" caused errors of up to ~0.5dex in the derived mass-loss rates. This has now been correcte

An extensive spectroscopic time-series of three Wolf-Rayet stars. I. The lifetime of large-scale structures in the wind of WR 134

During the summer of 2013, a 4-month spectroscopic campaign took place to observe the variabilities in three Wolf-Rayet stars. The spectroscopic data have been analyzed for WR 134 (WN6b), to better understand its behaviour and long-term periodicity, which we interpret as arising from corotating interaction regions (CIRs) in the wind. By analyzing the variability of the He II $\lambda$5411 emission line, the previously identified period was refined to P = 2.255 $\pm$ 0.008 (s.d.) days. The coherency time of the variability, which we associate with the lifetime of the CIRs in the wind, was deduced to be 40 $\pm$ 6 days, or $\sim$ 18 cycles, by cross-correlating the variability patterns as a function of time. When comparing the phased observational grayscale difference images with theoretical grayscales previously calculated from models including CIRs in an optically thin stellar wind, we find that two CIRs were likely present. A separation in longitude of $\Delta \phi \simeq$ 90$^{\circ}$ was determined between the two CIRs and we suggest that the different maximum velocities that they reach indicate that they emerge from different latitudes. We have also been able to detect observational signatures of the CIRs in other spectral lines (C IV $\lambda\lambda$5802,5812 and He I $\lambda$5876). Furthermore, a DAC was found to be present simultaneously with the CIR signatures detected in the He I $\lambda$5876 emission line which is consistent with the proposed geometry of the large-scale structures in the wind. Small-scale structures also show a presence in the wind, simultaneously with the larger scale structures, showing that they do in fact co-exist.Comment: 13 pages, 13 figures, 4 tables, will appear in the Monthly Notices for the Royal Astronomical Society, http://www.astro.umontreal.ca/~emily/CIR_Lifetime_WR134_full.pd

The Tarantula Massive Binary Monitoring

We present the first SB2 orbital solution and disentanglement of the massive Wolf-Rayet binary R145 (P = 159d) located in the Large Magellanic Cloud. The primary was claimed to have a stellar mass greater than 300Msun, making it a candidate for the most massive star known. While the primary is a known late type, H-rich Wolf-Rayet star (WN6h), the secondary could not be so far unambiguously detected. Using moderate resolution spectra, we are able to derive accurate radial velocities for both components. By performing simultaneous orbital and polarimetric analyses, we derive the complete set of orbital parameters, including the inclination. The spectra are disentangled and spectroscopically analyzed, and an analysis of the wind-wind collision zone is conducted. The disentangled spectra and our models are consistent with a WN6h type for the primary, and suggest that the secondary is an O3.5 If*/WN7 type star. We derive a high eccentricity of e = 0.78 and minimum masses of M1 sin^3 i ~ M2 sin^3 i ~ 13 +- 2 Msun, with q = M2 / M1 = 1.01 +- 0.07. An analysis of emission excess stemming from a wind-wind collision yields a similar inclination to that obtained from polarimetry (i = 39 +- 6deg). Our analysis thus implies M1 = 53^{+40}_{-20} and M2 = 54^{+40}_{-20} Msun, excluding M1 > 300Msun. A detailed comparison with evolution tracks calculated for single and binary stars, as well as the high eccentricity, suggest that the components of the system underwent quasi-homogeneous evolution and avoided mass-transfer. This scenario would suggest current masses of ~ 80 Msun and initial masses of Mi,1 ~ 105 and Mi,2 ~ 90Msun, consistent with the upper limits of our derived orbital masses, and would imply an age of ~2.2 Myr.Comment: Accepted for Publication in A&A, 16 pages, 17 figures and 4 table

Assembly and alignment of the 4-metre multi-object spectroscopic telescope wide field corrector

The 4-metre multi-object spectroscopic telescope (4MOST) is a fiber-fed multi-object spectrograph for the VISTA telescope at the European Southern Observatory (ESO) Paranal Observatory in Chile. The goal of the 4MOST project is to create a general purpose and highly efficient spectroscopic survey facility for astronomers in the 4MOST consortium and the ESO community. The instrument itself will record 2436 simultaneous spectra over a 1/44.2 square deg field of view and consists of an optical wide-field corrector (WFC), a fiber positioner system based on a tilting spine design, and three spectrographs giving both high and low spectral dispersion. The WFC comprises of six lenses grouped into four elements, two of which are cemented doublets that act as an atmospheric dispersion corrector. The first lens element is 0.9 m in diameter while the diameter of the other elements is 0.65 m. For the instrument to meet its science goals, each lens was aligned to be well within 1/4100 μm - a major challenge. This was achieved using contact metrology methods supplemented by pencil beam laser probes. In particular, an off-axis laser beam system has been implemented to test the optics' alignment before and after shipment. This work details the alignment and assembly methods and presents the latest results on the achieved lens positioning and projected performance of the WFC

Immunodetection of retinoblastoma-related protein and its phosphorylated form in interphase and mitotic alfalfa cells

Plant retinoblastoma-related (RBR) proteins are primarily considered as key regulators of G1/S phase transition, with functional roles in a variety of cellular events during plant growth and organ development. Polyclonal antibody against the C-terminal region of the Arabidopsis RBR1 protein also specifically recognizes the alfalfa 115 kDa MsRBR protein, as shown by the antigen competition assay. The MsRBR protein was detected in all cell cycle phases, with a moderate increase in samples representing G2/M cells. Antibody against the human phospho-pRb peptide (Ser807/811) cross-reacted with the same 115 kDa MsRBR protein and with the in vitro phosphorylated MsRBR protein C-terminal fragment. Phospho-MsRBR protein was low in G1 cells. Its amount increased upon entry into the S phase and remained high during the G2/M phases. Roscovitine treatment abolished the activity of alfalfa MsCDKA1;1 and MsCDKB2;1, and the phospho-MsRBR protein level was significantly decreased in the treated cells. Colchicine block increased the detected levels of both forms of MsRBR protein. Reduced levels of the MsRBR protein in cells at stationary phase or grown in hormone-free medium can be a sign of the division-dependent presence of plant RBR proteins. Immunolocalization of the phospho-MsRBR protein indicated spots of variable number and size in the labelled interphase nuclei and high signal intensity of nuclear granules in prophase. Structures similar to phospho-MsRBR proteins cannot be recognized in later mitotic phases. Based on the presented western blot and immunolocalization data, the possible involvement of RBR proteins in G2/M phase regulation in plant cells is discussed

Continuous-time modeling of cell fate determination in Arabidopsis flowers

<p>Abstract</p> <p>Background</p> <p>The genetic control of floral organ specification is currently being investigated by various approaches, both experimentally and through modeling. Models and simulations have mostly involved boolean or related methods, and so far a quantitative, continuous-time approach has not been explored.</p> <p>Results</p> <p>We propose an ordinary differential equation (ODE) model that describes the gene expression dynamics of a gene regulatory network that controls floral organ formation in the model plant <it>Arabidopsis thaliana</it>. In this model, the dimerization of MADS-box transcription factors is incorporated explicitly. The unknown parameters are estimated from (known) experimental expression data. The model is validated by simulation studies of known mutant plants.</p> <p>Conclusions</p> <p>The proposed model gives realistic predictions with respect to independent mutation data. A simulation study is carried out to predict the effects of a new type of mutation that has so far not been made in <it>Arabidopsis</it>, but that could be used as a severe test of the validity of the model. According to our predictions, the role of dimers is surprisingly important. Moreover, the functional loss of any dimer leads to one or more phenotypic alterations.</p