AMPK alpha 1-induced RhoA phosphorylation mediates vasoprotective effect of estradiol

OBJECTIVE: Estradiol (E2) mediates numerous beneficial effects assigned to estrogens, but whereas mechanisms have been described at the endothelial level, direct effects on vascular smooth muscle cells (VSMC) are poorly documented. As evidence accumulates regarding the role of RhoA in vascular pathophysiology and the benefit of RhoA-Rho associated protein kinase (Rock) pathway inhibition, we analyzed if E2 could inhibit it in VSMC. METHODS AND RESULTS: We show that in VSMC, E2 inhibits the RhoA-Rock pathway in a time- and concentration-dependent manner. The inhibition of RhoA-Rock pathway results from E2-induced phosphorylation of the Ser188 of RhoA. Using pharmacological, transfection, and in vitro phosphorylation experiments, we demonstrate that AMP-activated protein kinase subunit alpha 1 (AMPKalpha1) is activated by estrogen receptor stimulation and catalyzes RhoA phosphorylation induced by E2. Ex vivo, ovariectomy leads to an increase in the amplitude of phenylephrine- or serotonine-induced contractions of aortic rings in wild-type mice but not in AMPKalpha1-knock-out mice or E2-supplemented animals. These functional effects were correlated with a reduced level of RhoA phosphorylation in the aorta of ovariectomized female, male, and AMPKalpha1 knock-out mice. CONCLUSION: Our work thus defines AMPKalpha1 as (1) a new kinase for RhoA and (2) a new mediator of the vasoprotective effects of estrogen

The Spitzer-South Pole Telescope Deep Field: Survey Design and IRAC Catalogs

The Spitzer-South Pole Telescope Deep Field (SSDF) is a wide-area survey using Spitzer's Infrared Array Camera (IRAC) to cover 94 square degrees of extragalactic sky, making it the largest IRAC survey completed to date outside the Milky Way midplane. The SSDF is centered at 23:30,-55:00, in a region that combines observations spanning a broad wavelength range from numerous facilities. These include millimeter imaging from the South Pole Telescope, far-infrared observations from Herschel/SPIRE, X-ray observations from the XMM XXL survey, near-infrared observations from the VISTA Hemisphere Survey, and radio-wavelength imaging from the Australia Telescope Compact Array, in a panchromatic project designed to address major outstanding questions surrounding galaxy clusters and the baryon budget. Here we describe the Spitzer/IRAC observations of the SSDF, including the survey design, observations, processing, source extraction, and publicly available data products. In particular, we present two band-merged catalogs, one for each of the two warm IRAC selection bands. They contain roughly 5.5 and 3.7 million distinct sources, the vast majority of which are galaxies, down to the SSDF 5-sigma sensitivity limits of 19.0 and 18.2 Vega mag (7.0 and 9.4 microJy) at 3.6 and 4.5 microns, respectively.Comment: Accepted by ApJS; this version updated to conform to refereed articl

The XXL Survey VII: A supercluster of galaxies at z=0.43

The XXL Survey is the largest homogeneous and contiguous survey carried out with XMM-Newton. Covering an area of 50 square degrees distributed over two fields, it primarily investigates the large-scale structures of the Universe using the distribution of galaxy clusters and active galactic nuclei as tracers of the matter distribution. Given its depth and sky coverage, XXL is particularly suited to systematically unveiling the clustering of X-ray clusters and to identifying superstructures in a homogeneous X-ray sample down to the typical mass scale of a local massive cluster. A friends-of-friends algorithm in three-dimensional physical space was run to identify large-scale structures. In this paper we report the discovery of the highest redshift supercluster of galaxies found in the XXL Survey. We describe the X-ray properties of the clusters members of the structure and the optical follow-up. The newly discovered supercluster is composed of six clusters of galaxies at a median redshift z around 0.43 and distributed across approximately 30 by 15 arc minutes (10 by 5 Mpc on sky) on the sky. This structure is very compact with all the clusters residing in one XMM pointing; for this reason this is the first supercluster discovered with the XXL Survey. Spectroscopic follow-up with WHT (William Herschel Telescope) and NTT (New Technology Telescope) confirmed a median redshift of z = 0.43. An estimate of the X-ray mass and luminosity of this supercluster and of its total gas mass put XLSSC-e at the average mass range of superclusters; its appearance, with two members of equal size, is quite unusual with respect to other superclusters and provides a unique view of the formation process of a massive structure.Comment: A&A, accepted; special XXL issu

The XMM-LSS survey: the Class 1 cluster sample over the initial 5 square degrees and its cosmological modelling

We present a sample of 29 galaxy clusters from the XMM-LSS survey over an area of some 5deg2 out to a redshift of z=1.05. The sample clusters, which represent about half of the X-ray clusters identified in the region, follow well defined X-ray selection criteria and are all spectroscopically confirmed. For all clusters, we provide X-ray luminosities and temperatures as well as masses. The cluster distribution peaks around z=0.3 and T =1.5 keV, half of the objects being groups with a temperature below 2 keV. Our L-T(z) relation points toward self-similar evolution, but does not exclude other physically plausible models. Assuming that cluster scaling laws follow self-similar evolution, our number density estimates up to z=1 are compatible with the predictions of the concordance cosmology and with the findings of previous ROSAT surveys. Our well monitored selection function allowed us to demonstrate that the inclusion of selection effects is essential for the correct determination of the evolution of the L-T relation, which may explain the contradictory results from previous studies. Extensive simulations show that extending the survey area to 10deg2 has the potential to exclude the non-evolution hypothesis, but that constraints on more refined ICM models will probably be limited by the large intrinsic dispersion of the L-T relation. We further demonstrate that increasing the dispersion in the scaling laws increases the number of detectable clusters, hence generating further degeneracy [in addition to sigma8, Omega_m, L(M,z) and T(M,z)] in the cosmological interpretation of the cluster number counts. We provide useful empirical formulae for the cluster mass-flux and mass-count-rate relations as well as a comparison between the XMM-LSS mass sensitivity and that of forthcoming SZ surveys.Comment: Accepted for publication by MNRAS. Full resolution images as well as additional cluster data are available through a dedicated database at http://l3sdb.in2p3.fr:8080/l3sdb

Exploring the galaxy cluster-group transition regime at high redshifts: Physical properties of two newly detected z > 1 systems

Context: Multi-wavelength surveys for clusters of galaxies are opening a window on the elusive high-redshift (z>1) cluster population. Well controlled statistical samples of distant clusters will enable us to answer questions about their cosmological context, early assembly phases and the thermodynamical evolution of the intracluster medium. Aims: We report on the detection of two z>1 systems, XMMU J0302.2-0001 and XMMU J1532.2-0836, as part of the XMM-Newton Distant Cluster Project (XDCP) sample. We investigate the nature of the sources, measure their spectroscopic redshift and determine their basic physical parameters. Methods: The results of the present paper are based on the analysis of XMM-Newton archival data, optical/near-infrared imaging and deep optical follow-up spectroscopy of the clusters. Results: We confirm the X-ray source XMMU J0302.2-0001 as a gravitationally bound, bona fide cluster of galaxies at spectroscopic redshift z=1.185. We estimate its M500 mass to (1.6+/-0.3) times 10^{14} Msun from its measured X-ray luminosity. This ranks the cluster among intermediate mass system. In the case of XMMU J1532.2-0836 we find the X-ray detection to be coincident with a dynamically bound system of galaxies at z=1.358. Optical spectroscopy reveals the presence of a central active galactic nucleus, which can be a dominant source of the detected X-ray emission from this system. We provide upper limits of X-ray parameters for the system and discuss cluster identification challenges in the high-redshift low-mass cluster regime. A third, intermediate redshift (z=0.647) cluster, XMMU J0302.1-0000, is serendipitously detected in the same field as XMMU J0302.2-0001. We provide its analysis as well.Comment: Accepted to A&A, 13/04/2011. 15 pages, 18 figures, 5 tables, 2 appendice

The XMM large scale structure survey: optical vs. X-ray classifications of active galactic nuclei and the unified scheme

Our goal is to characterize AGN populations by comparing their X-ray and optical classifications. We present a sample of 99 spectroscopically identified X-ray point sources in the XMM-LSS survey which are significantly detected in the [2-10] keV band, and with more than 80 counts. We performed an X-ray spectral analysis for all of these 99 X-ray sources. Introducing the fourfold point correlation coefficient, we find only a mild correlation between the X-ray and the optical classifications, as up to 30% of the sources have differing X-ray and optical classifications: on one hand, 10% of the type 1 sources present broad emission lines in their optical spectra and strong absorption in the X-rays. These objects are highly luminous AGN lying at high redshift and thus dilution effects are totally ruled out, their discrepant nature being an intrinsic property. Their X-ray luminosities and redshifts distributions are consistent with those of the unabsorbed X-ray sources with broad emission lines. On the other hand, 25/32 are moderate luminosity AGN, which are both unabsorbed in the X-rays and only present narrow emission lines in their optical spectra. The majority of them have an optical spectrum which is representative of the host galaxy. We finally infer that dilution of the AGN by the host galaxy seems to account for their nature. 5/25 have been defined as Seyfert 2. In conclusion, most of these 32 discrepant cases can be accounted for by the standard AGN unified scheme, as its predictions are not met for only 12% of the 99 X-ray sources. ABRIDGEDComment: 25 pages, 19 figures, Accepted for publication in A&

The XMM-LSS catalogue: X-ray sources and associated optical data. Version I

Following the presentation of the XMM-LSS X-ray source detection package by Pacaud et al., we provide the source lists for the first 5.5 surveyed square degrees. The catalogues pertain to the [0.5-2] and [2-10] keV bands and contain in total 3385 point-like or extended sources above a detection likelihood of 15 in either band. The agreement with deep logN-logS is excellent. The main parameters considered are position, countrate, source extent with associated likelihood values. A set of additional quantities such as astrometric corrections and fluxes are further calculated while errors on the position and countrate are deduced from simulations. We describe the construction of the band-merged catalogue allowing rapid sub-sample selection and easy cross-correlation with external multi-wavelength catalogues. A small optical CFHTLS multi-band subset of objects is associated wich each source along with an X-ray/optical overlay. We make the full X-ray images available in FITS format. The data are available at CDS and, in a more extended form, at the Milan XMM-LSS database.Comment: 13 pages, 7 figures and 11 tables (fig. 1 and 6 are enclosed in reduced resolution), MNRAS Latex, accepted by MNRA

Bayesian Cluster Finder: Clusters in the CFHTLS Archive Research Survey

The detection of galaxy clusters in present and future surveys enables measuring mass-to-light ratios, clustering properties, galaxy cluster abundances and therefore, constraining cosmological parameters. We present a new technique for detecting galaxy clusters, which is based on the Matched Filter Algorithm from a Bayesian point of view. The method is able to determine the position, redshift and richness of the cluster through the maximization of a filter depending on galaxy luminosity, density and photometric redshift combined with a galaxy cluster prior that accounts for color-magnitude relations and BCG-redshift relation. We tested the algorithm through realistic mock galaxy catalogs, revealing that the detections are 100% complete and 80% pure for clusters up to z $$20 (Abell Richness $\sim$0, M$\sim4\times10^{14} M_{\odot}$). The completeness and purity remains approximately the same if we do not include the prior information, implying that this method is able to detect galaxy cluster with and without a well defined red sequence. We applied the algorithm to the CFHTLS Archive Research Survey (CARS) data, recovering similar detections as previously published using the same or deeper data plus additional clusters which appear to be real.Comment: Accepted for publication in MNRAS; 17 pages, 38 figure

The Observed Growth of Massive Galaxy Clusters I: Statistical Methods and Cosmological Constraints

(Abridged) This is the first of a series of papers in which we derive simultaneous constraints on cosmological parameters and X-ray scaling relations using observations of the growth of massive, X-ray flux-selected galaxy clusters. Our data set consists of 238 clusters drawn from the ROSAT All-Sky Survey, and incorporates extensive follow-up observations using the Chandra X-ray Observatory. Here we describe and implement a new statistical framework required to self-consistently produce simultaneous constraints on cosmology and scaling relations from such data, and present results on models of dark energy. In spatially flat models with a constant dark energy equation of state, w, the cluster data yield Omega_m=0.23 +- 0.04, sigma_8=0.82 +- 0.05, and w=-1.01 +- 0.20, marginalizing over conservative allowances for systematic uncertainties. These constraints agree well and are competitive with independent data in the form of cosmic microwave background (CMB) anisotropies, type Ia supernovae (SNIa), cluster gas mass fractions (fgas), baryon acoustic oscillations (BAO), galaxy redshift surveys, and cosmic shear. The combination of our data with current CMB, SNIa, fgas, and BAO data yields Omega_m=0.27 +- 0.02, sigma_8=0.79 +- 0.03, and w=-0.96 +- 0.06 for flat, constant w models. For evolving w models, marginalizing over transition redshifts in the range 0.05-1, we constrain the equation of state at late and early times to be respectively w_0=-0.88 +- 0.21 and w_et=-1.05 +0.20 -0.36. The combined data provide constraints equivalent to a DETF FoM of 15.5. Our results highlight the power of X-ray studies to constrain cosmology. However, the new statistical framework we apply to this task is equally applicable to cluster studies at other wavelengths.Comment: 16 pages, 7 figures. v4: final version (typographic corrections). Results can be downloaded at https://www.stanford.edu/group/xoc/papers/xlf2009.htm