Axonal amyloid precursor protein and its fragments undergo somatodendritic endocytosis and processing.

Deposition of potentially neurotoxic Aβ fragments derived from amyloid precursor protein (APP) at synapses may be a key contributor to Alzheimer's disease. However, the location(s) of proteolytic processing and subsequent secretion of APP fragments from highly compartmentalized, euploid neurons that express APP and processing enzymes at normal levels is not well understood. To probe the behavior of endogenous APP, particularly in human neurons, we developed a system using neurons differentiated from human embryonic stem cells, cultured in microfluidic devices, to enable direct biochemical measurements from axons. Using human or mouse neurons in these devices, we measured levels of Aβ, sAPPα, and sAPPβ secreted solely from axons. We found that a majority of the fragments secreted from axons were processed in the soma, and many were dependent on somatic endocytosis for axonal secretion. We also observed that APP and the β-site APP cleaving enzyme were, for the most part, not dependent on endocytosis for axonal entry. These data establish that axonal entry and secretion of APP and its proteolytic processing products traverse different pathways in the somatodendritic compartment before axonal entry

On the smallest scale for the incompressible Navier-Stokes equations

It is proven that for solutions to the two- and three-dimensional incompressible Navier-Stokes equations the minimum scale is inversely proportional to the square root of the Reynolds number based on the kinematic viscosity and the maximum of the velocity gradients. The bounds on the velocity gradients can be obtained for two-dimensional flows, but have to be assumed to be three-dimensional. Numerical results in two dimensions are given which illustrate and substantiate the features of the proof. Implications of the minimum scale result to the decay rate of the energy spectrum are discussed

Enabling Future Sustainability Transitions: An Urban Metabolism Approach to Los Angeles Pincetl et al. Enabling Future Sustainability Transitions

Summary: This synthesis article presents an overview of an urban metabolism (UM) approach using mixed methods and multiple sources of data for Los Angeles, California. We examine electric energy use in buildings and greenhouse gas emissions from electricity, and calculate embedded infrastructure life cycle effects, water use and solid waste streams in an attempt to better understand the urban flows and sinks in the Los Angeles region (city and county). This quantification is being conducted to help policy-makers better target energy conservation and efficiency programs, pinpoint best locations for distributed solar generation, and support the development of policies for greater environmental sustainability. It provides a framework to which many more UM flows can be added to create greater understanding of the study area's resource dependencies. Going forward, together with policy analysis, UM can help untangle the complex intertwined resource dependencies that cities must address as they attempt to increase their environmental sustainability

Nuclear Security Applications of Antineutrino Detectors: Current Capabilities and Future Prospects

Antineutrinos are electrically neutral, nearly massless fundamental particles produced in large numbers in the cores of nuclear reactors and in nuclear explosions. In the half century since their discovery, major advances in the understanding of their properties, and in detector technology, have opened the door to a new discipline: Applied Antineutrino Physics. Because antineutrinos are inextricably linked to the process of nuclear fission, many applications of interest are in nuclear nonproliferation. This white paper presents a comprehensive survey of applied antineutrino physics relevant for nonproliferation, summarizes recent advances in the field, describes the overlap of this nascent discipline with other ongoing fundamental and applied antineutrino research, and charts a course for research and development for future applications. It is intended as a resource for policymakers, researchers, and the wider nuclear nonproliferation community.Comment: This is a white paper on nonproliferation applications of antineutrino detectors. It will be cross posted to Physics and Society under the Physics sectio

Total cross sections for positrons scattered elastically from helium based on new measurements of total ionization cross sections

An improved technique is presented for employing the 2.3m spectrometer to measure total ionization cross sections, Q sub ion, for positrons incident on He. The new ionization cross section agree with the values reported earlier. Estimates are also presented of total elastic scattering cross section, Q sub el, obtained by subtracting from total scattering cross sections, Q sub tot, reported in the literature, the Q sub ion and Q sub Ps (total positronium formation cross sections) and total excitation cross sections, Q sub ex, published by another researcher. The Q sub ion and Q sub el measured with the 3m high resolution time-of-flight spectrometer for 54.9eV positrons are in accord with the results from the 2.3m spectrometer. The ionization cross sections are in fair agreement with theory tending for the most part to be higher, especially at 76.3 and 88.5eV. The elastic cross section agree quite well with theory to the vicinity of 50eV, but at 60eV and above the experimental elastic cross sections climb to and remain at about 0.30 pi a sub o sq while the theoretical values steadily decrease

Angra Neutrino Project: status and plans

We present the status and plans of the Angra Project, a new nuclear reactor neutrino oscillation experiment, proposed to be built in Brazil at the Angra dos Reis nuclear reactor complex. This experiment is aimed to measure theta_13, the last unknown of the three neutrino mixing angles. Combining a high luminosity design, very low background from cosmic rays and careful control of systematic errors at the 1% level, we propose a high sensitivity multi-detector experiment, able to reach a sensitivity to antineutrino disappearance down to sin^2(2*theta_13) = 0.006 in a three years running period, improving present limits constrained by the CHOOZ experiment by more than an order of magnitude.Comment: 2 pages, 1 figure, talk presented by J.C. Anjos ([email protected]) at NuFact05, 21-26 June 2005, Frascati, Ital

High-Throughput and Cost-Effective Characterization of Induced Pluripotent Stem Cells.

Reprogramming somatic cells to induced pluripotent stem cells (iPSCs) offers the possibility of studying the molecular mechanisms underlying human diseases in cell types difficult to extract from living patients, such as neurons and cardiomyocytes. To date, studies have been published that use small panels of iPSC-derived cell lines to study monogenic diseases. However, to study complex diseases, where the genetic variation underlying the disorder is unknown, a sizable number of patient-specific iPSC lines and controls need to be generated. Currently the methods for deriving and characterizing iPSCs are time consuming, expensive, and, in some cases, descriptive but not quantitative. Here we set out to develop a set of simple methods that reduce cost and increase throughput in the characterization of iPSC lines. Specifically, we outline methods for high-throughput quantification of surface markers, gene expression analysis of in vitro differentiation potential, and evaluation of karyotype with markedly reduced cost