Functional Analysis of Spontaneous Cell Movement under Different Physiological Conditions

Cells can show not only spontaneous movement but also tactic responses to environmental signals. Since the former can be regarded as the basis to realize the latter, playing essential roles in various cellular functions, it is important to investigate spontaneous movement quantitatively at different physiological conditions in relation to cellular physiological functions. For that purpose, we observed a series of spontaneous movements by Dictyostelium cells at different developmental periods by using a single cell tracking system. Using statistical analysis of these traced data, we found that cells showed complex dynamics with anomalous diffusion and that their velocity distribution had power-law tails in all conditions. Furthermore, as development proceeded, average velocity and persistency of the movement increased and as too did the exponential behavior in the velocity distribution. Based on these results, we succeeded in applying a generalized Langevin model to the experimental data. With this model, we discuss the relation of spontaneous cell movement to cellular physiological function and its relevance to behavioral strategies for cell survival.Comment: Accepted to PLoS ON

Fluctuation theorem applied to Dictyostelium discoideum system

In this paper, we analyze the electrotactic movement of Dictyostelium discoideum from the viewpoint of non-equilibrium statistical mechanics. Because we can observe fluctuating behavior of cellular trajectories, we analyze the probability distribution of the trajectories with the aid of the fluctuation theorem. Recently, the validity of the fluctuation theorem was verified in a colloidal system, and it has also been applied to granular systems, turbulent systems and chemical oscillatory waves to investigate some of their statistical properties that are not yet completely understood. Noting that the fluctuation theorem is potentially applicable to cellular electrotaxis, here we employ it to help us obtain a phenomenological model of this biological system.Comment: 2 pages, to appear in J. Phys. Soc. Jp

Active Brownian Particles. From Individual to Collective Stochastic Dynamics

We review theoretical models of individual motility as well as collective dynamics and pattern formation of active particles. We focus on simple models of active dynamics with a particular emphasis on nonlinear and stochastic dynamics of such self-propelled entities in the framework of statistical mechanics. Examples of such active units in complex physico-chemical and biological systems are chemically powered nano-rods, localized patterns in reaction-diffusion system, motile cells or macroscopic animals. Based on the description of individual motion of point-like active particles by stochastic differential equations, we discuss different velocity-dependent friction functions, the impact of various types of fluctuations and calculate characteristic observables such as stationary velocity distributions or diffusion coefficients. Finally, we consider not only the free and confined individual active dynamics but also different types of interaction between active particles. The resulting collective dynamical behavior of large assemblies and aggregates of active units is discussed and an overview over some recent results on spatiotemporal pattern formation in such systems is given.Comment: 161 pages, Review, Eur Phys J Special-Topics, accepte

Powder Compaction: Compression Properties of Cellulose Ethers

Effective development of matrix tablets requires a comprehensive understanding of different raw material attributes and their impact on process parameters. Cellulose ethers (CE) are the most commonly used pharmaceutical excipients in the fabrication of hydrophilic matrices. The innate good compression and binding properties of CE enable matrices to be prepared using economical direct compression (DC) techniques. However, DC is sensitive to raw material attributes, thus, impacting the compaction process. This article critically reviews prior knowledge on the mechanism of powder compaction and the compression properties of cellulose ethers, giving timely insight into new developments in this field

Crustal exhumation and depocenter migration from the Alpine orogenic margin towards the Pannonian extensional back-arc basin controlled by inheritance

The formation and deformation history of back-arc basins play a critical role in understanding the tectonics of plate interactions. Furthermore, opening of extensional back-arc basins during the overall convergence between Africa and Europe is a fundamental process in the overall tectonic evolution of the Mediterranean and adjacent areas. In this frame, Miocene tectonic evolution of the western Pannonian Basin of Central Europe and its connection to inherited Cretaceous structures of the Eastern Alpine nappes are presented. Revision of published and addition of new structural and thermochronological data, as well as seismic profiles from the western Pannonian Basin is complemented by high-resolution thermo-mechanical numerical modeling in order to propose a new physically consistent tectono-sedimentary model for the basin evolution. The onset of extension is dated as ~25–23 Ma, and higher rates are inferred between 19 and 15 Ma at the south-western part of the area (Pohorje, Kozjak Domes, Murska Sobota Ridge, and Mura-Zala Basin). Rift initiation involved the exhumation of the middle part of the Austroalpine nappe pile along low-angle detachment faults and mylonite zones. The Miocene low-angle shear zones could reactivate major Cretaceous thrust boundaries, the exhumation channel of ultra-high-pressure rocks of the Pohorje Dome, or Late Cretaceous extensional structures. Miocene extension was associated with granodiorite and dacite intrusions between 18.64 and 15 Ma. The Pohorje pluton intruded at variable depth from ~4 to 16–18 km and experienced ductile stretching, westward tilting, and asymmetric exhumation of its eastern side. Terrestrial early Miocene (Ottnangian to Karpatian, 19–17.25 Ma) syn-rift depositional environment in supradetachment basins evolved to near-shore and bathyal one by the middle Miocene (Badenian, 15.97–12.8 Ma). Deformation subsequently migrated eastwards to the western part of the Transdanubian Range (Keszthely Hills) and to newly formed grabens. In this formerly emerged terrestrial area active faulting started at 15–14.5 Ma and continued through the late Miocene almost continuously up to ~8 Ma but basically terminated in the Mura-Zala Basin by ~15 Ma (early Badenian). These observations suggest a ~200 km shift of active faulting, basin formation, and related syn-tectonic sedimentation from the SW (Pohorje and Mura-Zala Basin) toward the Pannonian Basin center. Building on the above described observational and modeling data makes the Pannonian Basin an ideal natural laboratory for understanding the coupling between deep Earth and surface processes.ISSN:0921-818

A Quorum-Sensing Factor in Vegetative Dictyostelium Discoideum Cells Revealed by Quantitative Migration Analysis

Background: Many cells communicate through the production of diffusible signaling molecules that accumulate and once a critical concentration has been reached, can activate or repress a number of target genes in a process termed quorum sensing (QS). In the social amoeba Dictyostelium discoideum, QS plays an important role during development. However little is known about its effect on cell migration especially in the growth phase. Methods and Findings: To investigate the role of cell density on cell migration in the growth phase, we use multisite timelapse microscopy and automated cell tracking. This analysis reveals a high heterogeneity within a given cell population, and the necessity to use large data sets to draw reliable conclusions on cell motion. In average, motion is persistent for short periods of time (tƒ5min), but normal diffusive behavior is recovered over longer time periods. The persistence times are positively correlated with the migrated distances. Interestingly, the migrated distance decreases as well with cell density. The adaptation of cell migration to cell density highlights the role of a secreted quorum sensing factor (QSF) on cell migration. Using a simple model describing the balance between the rate of QSF generation and the rate of QSF dilution, we were able to gather all experimental results into a single master curve, showing a sharp cell transition between high and low motile behaviors with increasing QSF. Conclusion: This study unambiguously demonstrates the central role played by QSF on amoeboid motion in the growt