Investigation of environmental change pattern in Japan: Investigation of variations in the prominent oceanic current, Kuroshio

The author has identified the following significant results. In the print of MSS band 4 of Shiono Misaki and Ise taken by LANDSAT on 11 September 1975, different water masses can be recognized distinctly. At 35 miles off Shiono Misaki, a vortex pattern is found which has an ellipsoidal shape with a diameter of 30 miles and appears to rotate counterclockwise. In Ise, water masses were found spreading widely along the coast from the mouth of Ise Bay. The temperature of sea surface obtained from sea truth shows almost a uniform pattern. The distribution of surface salinity and transparency shows a distinct pattern

Investigation of environmental change pattern in Japan. Investigation of variations in the prominent oceanic current, Kuroshio

The author has identified the following significant results. It was confirmed that band 4 and 5 had some information as to the sea surface, such as spreading of coastal waters or eddy-like motion of surface water. It was concluded that the front line along the coast from Kumano-nada to Shiono-misaki in the monochromatic prints and in the pseudocolor images was a boundary between coastal waters and offshore water. Most of Ise Bay was covered by polluted water, having salinity values less than 30%, high water color scale of 6 to 8, and transparency less than 5 m

Investigation of environmental change pattern in Japan. Investigation of variations in the prominent oceanic current, Kuroshio

The author has identified the following significant results. Rias shorelines are interpreted from the fine depiction of their complex features in the image of band 7. Sand beaches are discriminated from their linear nature, and the similarity of sand beaches among the all band is very good

Investigation of environmental change pattern in Japan. Classification of shorelines

The author has identified the following significant results. The sand beach was separated from sea water in each of four bands, if the beach had a width of 100 m or more. Density ranges of the sea for CCT counts were determined as 0-3 for band 7, 0-16 for band 6, 0-25 for band 5, and 0-27 for band 4

Self-learning Multiscale Simulation for Achieving High Accuracy and High Efficiency Simultaneously

We propose a new multi-scale molecular dynamics simulation method which can achieve high accuracy and high sampling efficiency simultaneously without aforehand knowledge of the coarse grained (CG) potential and test it for a biomolecular system. Based on the resolution exchange simulations between atomistic and CG replicas, a self-learning strategy is introduced to progressively improve the CG potential by an iterative way. Two tests show that, the new method can rapidly improve the CG potential and achieve efficient sampling even starting from an unrealistic CG potential. The resulting free energy agreed well with exact result and the convergence by the method was much faster than that by the replica exchange method. The method is generic and can be applied to many biological as well as non-biological problems.Comment: 14 pages, 6 figure

Quantitation of intracellular NAD(P)H can monitor an imbalance of DNA single strand break repair in base excision repair deficient cells in real time

DNA single strand breaks (SSBs) are one of the most frequent DNA lesions in genomic DNA generated either by oxidative stress or during the base excision repair pathways. Here we established a new real-time assay to assess an imbalance of DNA SSB repair by indirectly measuring PARP-1 activation through the depletion of intracellular NAD(P)H. A water-soluble tetrazolium salt is used to monitor the amount of NAD(P)H in living cells through its reduction to a yellow colored water-soluble formazan dye. While this assay is not a direct method, it does not require DNA extraction or alkaline treatment, both of which could potentially cause an artifactual induction of SSBs. In addition, it takes only 4 h and requires less than a half million cells to perform this measurement. Using this assay, we demonstrated that the dose- and time-dependent depletion of NAD(P)H in XRCC1-deficient CHO cells exposed to methyl methanesulfonate. This decrease was almost completely blocked by a PARP inhibitor. Furthermore, methyl methanesulfonate reduced NAD(P)H in PARP-1+/+cells, whereas PARP-1¿/¿ cells were more resistant to the decrease in NAD(P)H. These results indicate that the analysis of intracellular NAD(P)H level using water-soluble tetrazolium salt can assess an imbalance of SSB repair in living cells in real time

Competing Ground States of a Peierls-Hubbard Nanotube

Motivated by iodo platinum complexes assembled within a quadratic-prism lattice, [Pt(C$_2$H$_8$N$_2$)(C$_{10}$H$_8$N$_2$)I]$_4$(NO$_3$)$_8$, we investigate the ground-state properties of a Peierls-Hubbard four-legged tube. Making a group-theoretical analysis, we systematically reveal a variety of valence arrangements, including half-metallic charge-density-wave states. Quantum and thermal phase competition is numerically demonstrated with particular emphasis on doping-induced successive insulator-to-metal transitions with conductivity increasing stepwise.Comment: 6 pages, 4 figures. to be published in Europhys. Lett. 87 (2009) 1700

Cryobanking potentials of marine shrimp gametes

For developing marine shrimp industry through aquaculture technology, one of the major constraints is non-availability of sufficient seed and spawners to produce seed at the desired time. Cryopreservation of gametes of aquatic animals in contrast to the situation in other vertebrates particularly mammals has met with a very limited success. Very few attempts have been made on cryopreservation of sperms in decapod crustaceans in general and marine shrimps in particular. Efforts are now being made continuously on cryopreservation and cryobanking of penaeid gametes. The viable spermatozoa of P. indicus and P. monodon havebeen successfully preserved in CMFRI