Performance tests on Screw Feeder Conveyor for Nodule Transfer Deep Sea Applications

A screw conveyor is a versatile conveyor used in many process plants for the transfer of bulk solids and powders. The following article has written to understand screw conveyor design and selection of the right screw conveyor for underwater applications to convey crushed manganese nodules to the pump system for nodule transfer. The nodules from the sea bed will be collected by a pickup device and crushed into less than 30mm pieces by using a crusher and pumped by a positive displacement pump to the mother vessel. Screw feeder is used to transfer curshed Polymetallic Nodules from hopper to pump system with controlled feed rate. A land based screw feeder is modified to suit the under water applications. Studies on screw conveyors were conducted to examine performance in land as well as underwater. Most of these studies were experimental in nature. This paper presents a critical review on the design and validation of a screw conveyor

Synthetic Studies in Phytochrome Chemistry

An account is given of the author’s several approaches to the synthesis of the parent chromophore of phytochrome (1), a protein-bound linear tetrapyrrole derivative that controls photomorphogenesis in higher plants. These studies culminated in enantioselective syntheses of both (2R)- and (2S)-phytochromobilin (4), as well as several 13C-labeled derivatives designed to probe the site of Z,E-isomerization during photoexcitation. When reacted in vitro, synthetic 2R-4 and recombinant-derived phytochrome apoprotein N-C produced a protein-bound chromophore with identical difference spectra to naturally occurring 1

Tracking with Sobolev active contours

Recently proposed Sobolev active contours introduced a new paradigm for minimizing energies defined on curves by changing the traditional cost of perturbing a curve and thereby redefining their gradients. Sobolev active contours evolve more globally and are less attracted to certain intermediate local minima than traditional active contours. In this paper we analyze Sobolev active contours in the Fourier domain in order to understand their evolution across different scales. This analysis shows an important and useful behavior of Sobolev contours, namely, that they move successively from coarse to increasingly finer scale motions in a continuous manner. Along with other properties, the previous observation reveals that Sobolev active contours are ideally suited for tracking problems that use active contours. Our purpose in this work is to show how a variety of active contour based tracking methods can be significantly improved merely by evolving the active contours according to the Sobolev method. 1

Reduced postoperative blood loss and transfusion requirement after beating-heart coronary operations: A prospective randomized study

AbstractObjective: Coronary artery bypass grafting on the beating heart through median sternotomy is a relatively new treatment, which allows multiple revascularization without the use of cardiopulmonary bypass. A prospective randomized study was designed to investigate the effect of coronary bypass with or without cardiopulmonary bypass on postoperative blood loss and transfusion requirement. Methods: Two hundred patients with coronary artery disease were prospectively randomized to (1) on-pump treatment with conventional cardiopulmonary bypass and cardioplegic arrest and (2) off-pump treatment on the beating heart. Postoperative blood loss identified as total chest tube drainage, transfusion requirement, and related costs together with hematologic indices and clotting profiles were analyzed. Results: There was no difference between the groups with respect to preoperative and intraoperative patient variables. The mean ratio of postoperative blood loss and 95% confidence interval between groups was 1.64 and 1.39 to 1.94, respectively, suggesting on average a postoperative blood loss 1.6 times higher in the on-pump group compared with the off-pump group. Seventy-seven patients in the off-pump group required no blood transfusion compared with only 48 in the on-pump group (P <.01). Furthermore, less than 5% of patients in the on-pump group required fresh frozen plasma and platelet transfusion compared with 30% and 25%, respectively, in the on-pump group (both P <.05). Mean transfusion cost per patient was higher in the on-pump compared with that in the off-pump group ($184.8 ±$35.2 vs $21.47 ±$6.9, P <.01). Conclusions: Coronary artery bypass grafting on the beating heart is associated with a significant reduction in postoperative blood loss, transfusion requirement, and transfusion-related cost when compared with conventional revascularization with cardiopulmonary bypass and cardioplegic arrest. (J Thorac Cardiovasc Surg 2001;121:689-96

Intraflagellar transport proteins are involved in thrombocyte filopodia formation and secretion

Intraflagellar transport (IFT) proteins are vital for the genesis and maintenance of cilia. Our identification of ift122 transcripts in zebrafish thrombocytes that lack primary cilia was unexpected. IFT proteins serve transport in cilia, whose narrow dimensions may have necessitated the evolution of IFT from vesicular transport in ancestral eukaryotes. We hypothesized that IFTs might also facilitate transport within the filopodia that form when thrombocytes are activated. To test this possibility, we knocked down ift122 expression by injecting antisense Morpholino oligonucleotides (MOs) into zebrafish embryos. Laser-induced arterial thrombosis showed prolonged time to occlusion (TTO) of the vessel, as would be expected with defective thrombocyte function. Acute effects in adult zebrafish were evaluated by Vivo-Morpholino (Vivo-MO) knockdown of ift122. Vivo-MO morphants showed a prolonged time to thrombocyte aggregation (TTA) in the plate tilt assay after thrombocyte activation by the following agonists: ADP, collagen, PAR1 peptide, and epinephrine. A luminescence assay for ATP revealed that ATP secretion by thrombocytes was reduced in collagen-activated blood of Vivo-MO ift122 morphants. Moreover, DiI-C18 labeled morphant thrombocytes exposed to collagen showed reductions in filopodia number and length. Analysis of ift mutants, in which cilia defects have been noted, also showed prolongation of TTO in our arterial laser thrombosis assay. Additionally, collagen activation of wild-type thrombocytes led to a concentration of IFT122 both within and at the base of filopodia. Taken together these results, suggest that IFT proteins are involved in both the extension of filopodia and secretion of ATP, which are critical in thrombocyte function