Prevalence of malarial parasites in pregnant women attending Sir Muhammad Sunusi Specialist Hospital, Kano, Nigeria

A total of 300 blood samples of pregnant women were tested to determine the prevalence of malarial parasite using Leishman’s stain method. Out of which, 155 (51.7%) were found to be M.P positive, while 145 (48.3%) were M.P negative. 85 (54.8%) of M.P positive had the highest percentage as the primigravidae followed by 70 (45.16%) as multigravidae. Observation have shown that there was a high prevalence of 76 (49.03%) positive patients in the month of August followed by September with 44 (34.19%) as well as 26(16.77%) in July respectively. This was attributed as a result of heavy rainfall experience between Julys to September. It was also seen that patients from Sauna quarters has a highest number of prevalence of 36 (24.5%). this attributed to the fact that they have a close proximity with the hospital and has a highest number of patients that attended the antenatal clinic during the period. Keyword: Prevalence, Malarial Parasite, Pregnant Women, Kano

Evaluation of antimicrobial activities of Combretum micranthum L.

The antibacterial and antifungal activities of extracts of Combretum micranthum L. (root) were tested against Pseudomonas aeruginosa, Klebsiella pneumoniae, Streptococcus pneumoniae, Salmonella typhi, Staphylococcus aureus, Escherichia coli and Candida albicans respectively. The plant (root) extracts were obtained using ethanol, chloroform, ethyl acetate and distilled water solvents. All the test organisms were studied using the Agar Diffusion Method (ADM) and were susceptible to crude and ethyl acetate extracts but Escherichia coli and Candida albicans were resistant. The extracts of Combretum micranthum L. had broader spectrum of activity than the commercial antibiotics tested against isolates. Results of the study confirmed the chemotherapeutic values in ethnomedicine of extracts of this plant in the treatment of various ailments. Key words: Antimicrobial, Extracts, Combretum micranthu

A Study of the Phytochemical Properties and Synergistic Action of Leaf Extracts of Dodonea Viscosa Linn, Annona Comosus (Linn) Merr Peel and Citrus Senensis Peel on Aeromonas hydrophila and Salmonella Species

The leaf of Dodonea viscosa Linn, peels of Annona comosus (Linn) Merr and Citrus senensis are widely used traditional remedies against various ailments, such as digestive system disorders like: indigestion, ulcers, diarrhoea, constipation, upset stomach and tonic to digestive system. The major chemical constituents reported from the plant parts are alkaloids, flavoniods, saponins, tannins, steroids, triterpenoids and phytosterols which show that these plant parts can be a potential candidate to be use as a therapeutic agent. The aim of the study was to determine the synergism at a concentration ratio of 1:1 between the three extracts using discs diffusion, broth tube dilution and fractional inhibitory concentration techniques against six Salmonella paratyphi B, one Salmonella typhi and three A. hydrophila. In vitro anti-salmonellae and A. hydrophila activities of the extracts were confirmed and no synergism was demonstrated (P = 0.05). Keywords: Bioactivity, phytochemicals, synergism, Dodonea viscosa, Annona comosus, Citrus senensis, MIC, MBC, FIC

Stroke-associated pattern of gene expression previously identified by machine-learning is diagnostically robust in an independent patient population

Our group recently employed genome-wide transcriptional profiling in tandem with machine-learning based analysis to identify a ten-gene pattern of differential expression in peripheral blood which may have utility for detection of stroke. The objective of this study was to assess the diagnostic capacity and temporal stability of this stroke-associated transcriptional signature in an independent patient population. Publicly available whole blood microarray data generated from 23 ischemic stroke patients at 3, 5, and 24 h post-symptom onset, as well from 23 cardiovascular disease controls, were obtained via the National Center for Biotechnology Information Gene Expression Omnibus. Expression levels of the ten candidate genes (ANTXR2, STK3, PDK4, CD163, MAL, GRAP, ID3, CTSZ, KIF1B, and PLXDC2) were extracted, compared between groups, and evaluated for their discriminatory ability at each time point. We observed a largely identical pattern of differential expression between stroke patients and controls across the ten candidate genes as reported in our prior work. Furthermore, the coordinate expression levels of the ten candidate genes were able to discriminate between stroke patients and controls with levels of sensitivity and specificity upwards of 90% across all three time points. These findings confirm the diagnostic robustness of the previously identified pattern of differential expression in an independent patient population, and further suggest that it is temporally stable over the first 24 h of stroke pathology

Conversion of biomass to adsorbent: a review

Tons of biomass are produced every year including organic agricultural and forestry by-products but they are of limited value. Mostly, in the developing countries, the biomasses are considered as waste and are being burnt or thrown to liter the environment as part of teaming solid waste. Presently, there are no sustainable long-term management strategies to use biomass. The utilization of biomass to produce activated carbon is a good approach that is industrially useful and environmentally benign materials. The adsorption technique is using adsorbents in the removal of heavy metals from water therefore, biomass can be converted to the adsorbent and utilized as a waste-to-wealth commodity in water purification. In this review, the suitable process for conversion of biomass to cheap and simple means of obtaining activation carbon as adsorbent is presented. The potential uses of biomass and the conversion stages including carbonization, pyrolysis, gasification, and activation were discussed. This work depicts that the issue of solid waste utilization to solve existing issues with locally available and cheap materials is beneficial to man and the environment

HIV Protease Inhibitors Sensitize Human Head and Neck Squamous Carcinoma Cells to Radiation by Activating Endoplasmic Reticulum Stress

Background Human head and neck squamous cell carcinoma (HNSCC) is the sixth most malignant cancer worldwide. Despite significant advances in the delivery of treatment and surgical reconstruction, there is no significant improvement of mortality rates for this disease in the past decades. Radiotherapy is the core component of the clinical combinational therapies for HNSCC. However, the tumor cells have a tendency to develop radiation resistance, which is a major barrier to effective treatment. HIV protease inhibitors (HIV PIs) have been reported with radiosensitizing activities in HNSCC cells, but the underlying cellular/molecular mechanisms remain unclear. Our previous study has shown that HIV PIs induce cell apoptosis via activation of endoplasmic reticulum (ER) stress. The aim of this study was to examine the role of ER stress in HIV PI-induced radiosensitivity in human HNSCC. Methodology and Principal Findings HNSCC cell lines, SQ20B and FaDu, and the most commonly used HIV PIs, lopinavir and ritonavir (L/R), were used in this study. Clonogenic assay was used to assess the radiosensitivity. Cell viability, apoptosis and cell cycle were analyzed using Cellometer Vision CBA. The mRNA and protein levels of ER stress-related genes (eIF2α, CHOP, ATF-4, and XBP-1), as well as cell cycle related protein, cyclin D1, were detected by real time RT-PCR and Western blot analysis, respectively. The results demonstrated that L/R dose-dependently sensitized HNSCC cells to irradiation and inhibited cell growth. L/R-induced activation of ER stress was correlated to down-regulation of cyclin D1 expression and cell cycle arrest under G0/G1 phase. Conclusion and Significance HIV PIs sensitize HNSCC cells to radiotherapy by activation of ER stress and induction of cell cycle arrest. Our results provided evidence that HIV PIs can be potentially used in combination with radiation in the treatment of HNSCC

Monocyte-lymphocyte cross-communication via soluble CD163 directly links innate immune system activation and adaptive immune system suppression following ischemic stroke

CD163 is a scavenger receptor expressed on innate immune cell populations which can be shed from the plasma membrane via the metalloprotease ADAM17 to generate a soluble peptide with lympho-inhibitory properties. The purpose of this study was to investigate CD163 as a possible effector of stroke-induced adaptive immune system suppression. Liquid biopsies were collected from ischemic stroke patients (n = 39), neurologically asymptomatic controls (n = 20), and stroke mimics (n = 20) within 24 hours of symptom onset. Peripheral blood ADAM17 activity and soluble CD163 levels were elevated in stroke patients relative to non-stroke control groups, and negatively associated with post-stroke lymphocyte counts. Subsequent in vitro experiments suggested that this stroke-induced elevation in circulating soluble CD163 likely originates from activated monocytic cells, as serum from stroke patients stimulated ADAM17-dependant CD163 shedding from healthy donor-derived monocytes. Additional in vitro experiments demonstrated that stroke-induced elevations in circulating soluble CD163 can elicit direct suppressive effects on the adaptive immune system, as serum from stroke patients inhibited the proliferation of healthy donor-derived lymphocytes, an effect which was attenuated following serum CD163 depletion. Collectively, these observations provide novel evidence that the innate immune system employs protective mechanisms aimed at mitigating the risk of post-stroke autoimmune complications driven by adaptive immune system overactivation, and that CD163 is key mediator of this phenomenon

Machine-Learning Approach Identifies a Pattern of Gene Expression in Peripheral Blood that can Accurately Detect Ischaemic Stroke

Early and accurate diagnosis of stroke improves the probability of positive outcome. The objective of this study was to identify a pattern of gene expression in peripheral blood that could potentially be optimised to expedite the diagnosis of acute ischaemic stroke (AIS). A discovery cohort was recruited consisting of 39 AIS patients and 24 neurologically asymptomatic controls. Peripheral blood was sampled at emergency department admission, and genome-wide expression profiling was performed via microarray. A machine-learning technique known as genetic algorithm k-nearest neighbours (GA/kNN) was then used to identify a pattern of gene expression that could optimally discriminate between groups. This pattern of expression was then assessed via qRT-PCR in an independent validation cohort, where it was evaluated for its ability to discriminate between an additional 39 AIS patients and 30 neurologically asymptomatic controls, as well as 20 acute stroke mimics. GA/kNN identified 10 genes (ANTXR2, STK3, PDK4, CD163, MAL, GRAP, ID3, CTSZ, KIF1B and PLXDC2) whose coordinate pattern of expression was able to identify 98.4% of discovery cohort subjects correctly (97.4% sensitive, 100% specific). In the validation cohort, the expression levels of the same 10 genes were able to identify 95.6% of subjects correctly when comparing AIS patients to asymptomatic controls (92.3% sensitive, 100% specific), and 94.9% of subjects correctly when comparing AIS patients with stroke mimics (97.4% sensitive, 90.0% specific). The transcriptional pattern identified in this study shows strong diagnostic potential, and warrants further evaluation to determine its true clinical efficacy

Selection of properties as references for ecological restoration in Brazilian Semiarid.

This study aimed to select 2 properties per city for pilot implementation restoration ecology in two ways. Firstly, it was defined a series criteria for choosing areas through GIS techniques. After, questionnaires were applied for selecting the properties in the cities