Pengaruh Pelatihan Dan Pengembangan Sumber Daya Manusia Terhadap Kinerja Pegawai Yang Di Mediasi Oleh Semangat Kerja

Penelitian ini bertujuan untuk menguji dan menganalisis pengaruh pelatihan dan pengembangan sumber daya manusia terhadap kinerja pegawai yang dimediasi olehsemangat kerja pada PT Bank Pembangunan Daerah Papua Cabang Utama Jayapura. Metode penelitian yang digunakan dalam penelitian ini adalah dengan teknik analisis deskriptif dan menggunakan alat uji statistik smartPLS 3 dengan metode analisis partial least square.Hasil penelitian ini menunjukkan bahwa secara langsung terdapat pengaruh pelatihan terhadap kinerja pegawai, pengembangan sumber daya manusia berpengaruh positif dan signifikan terhadap kinerja pegawai, pelatihan berpengaruh positif dan signifikan terhadap semangat kerja, pengembangan sumber daya manusia berpengaruh positif dan signifikan terhadap semangat kerja, semangat kerja berpengaruh positif dan signifikan terhadap kinerja pegawai. Selanjutnya secara tidak langsung menunjukkan bahwa pelatihan terhadap kinerjapegawai yang dimediasi variabel semangat kerja berpengaruh positif yang signifikan, pengembangan sumber daya manusia terhadap kinerja pegawai yang dimediasi variabel semangat kerja berpengaruh positif yang signifikan

NOS3 gene variants and male infertility: Association of 4a/4b with oligoasthenozoospermia

Results of recent studies confirmed that oxidative stress negatively affects sperm motility and causes sperm DNA damage. Produced by nitric oxide synthase 3 (NOS3), nitric oxide is considered to be one of the important mediators of oxidative stress in testis tissue. The aim of this study was to assess the possible association of three genetic variants (rs2070744, rs1799983 and intron variant 4a/4b) in NOS3 gene and infertility occurrence in two groups of infertile men (idiopathic azoospermia and oligoasthenozoospermia) and fertile controls. Genotypes for the single-nucleotide genetic variants rs1799983 and rs2070744 were determined by PCR-RFLP, while genotyping of intron 4 variant 4a/4b was performed by gel electrophoresis of PCR products. Statistical analysis was performed by SNPStats software. No significant association between the three genetic variants of the NOS3 gene and infertility risk was determined comparing allele and genotype frequencies among group of patients diagnosed with azoospermia and the control group. Nevertheless, there was a significant positive association between 4a/4b and infertility in the group of males diagnosed with oligoasthenozoospermia, under overdominant genetic model. Our findings suggest that tandem repeat variant within intron 4 of the NOS3 gene is associated with an increased risk of infertility in men diagnosed with idiopathic oligoasthenozoospermia.Andrologia (2017): e1281

Screening of mutations and polymorphism in CFRT gene in men infertile due to oligo- or azospermia

Do sada je otkriven veliki broj gena koji, kada su mutirani ili deletirani uzrokuju promene u muškom reprodukcionom sistemu. Opstrukciona azospermija, koja je uzrok infertiliteta, u šest posto slučajeva posledica je kongenitalnog nedostatka vaza deferensa. Takođe, kongenitalni nedostatak vaza deferensa se javlja kod 95 posto muškaraca obolelih od cistične fibroze, koja nastaje usled mutacija u genu CFTR. Novija istraživanja pokazuju da je kod muškaraca infertilnih usled kongenitalnog nedostatka vaza deferensa, bez kliničkih znakova cistične fibroze, povećana učestalost mutacija u genu CFTR u odnosu na učestalost mutiranog gena CFTR u opštoj populaciji. Pošto je kod muškaraca obolelih od cistične fibroze nađen širok spektar oštećenja reprodukcionog trakta odlučili smo se za analizu mutacija i polimorfizama u genu CFTR kod muškaraca, infertilnih usled oligospermije ili azospermije, radi rasvetljavanja moguće uloge gena CFTR u patogenezi infertilnosti muškaraca. U grupi osoba s opstrukcionom azospermijom otkrili smo statistički značajno veću učestalost mutacija u genu CFTR nego u opštoj populaciji, što ukazuje na njegovo učešće u patologiji infertilnosti kod ove trupe ispitanika. U grupi muškaraca s poremećajem u spermatogenezi ili sazrevanju sperme učestalost mutacija u genu CFTR takođe je bila veća nego u opštoj populaciji, ali niža nego kod ispitanika s opstrukcionom azospermijom. Ovo ukazuje na veće učešće drugih gena u procesu spermatogeneze nego kod opstrukcione azospermije. S obzirom da je kod muškaraca infertilnih usled opstrukcione azospermije veća učestalost mutacija u genu CFTR, oni su s većim rizikom za rađanje deteta s cističnom fibrozom, te je kod njih indikovana analiza mutacija u ovom genu pre pristupanja asistiranoj reprodukciji.We concluded that CFTR gene plays a role in the etiology of obstructive azoospermia and that it also could be involved in same cases of impaired spermatogenesis and sperm maturation. Due to the high incidence of CFRT mutations in patients with obstructive azoospermia we suggest screening of CFRT mutations before assisted reproduction

Screening of mutations and polymorphism in CFRT gene in men infertile due to oligo- or azospermia

We concluded that CFTR gene plays a role in the etiology of obstructive azoospermia and that it also could be involved in same cases of impaired spermatogenesis and sperm maturation. Due to the high incidence of CFRT mutations in patients with obstructive azoospermia we suggest screening of CFRT mutations before assisted reproduction

Electric Potential Induced Prevention and Removal of an Algal Biofoulant from Planar SERS Substrates

Ulva zoospores are widespread marine macroalgae and a common organism found in biofouling communities due to their strong adhesive properties and quick settlement times. Using Ulva as a model organism, a strategy is presented where direct-current (DC) electric potentials are applied in conjunction with surface-enhanced Raman spectroscopy (SERS) to characterize, remove, and prevent Ulva from forming a biofilm on gold-capped nanopillar SERS substrates. Experiments were conducted within a poly­(tetrafluoroethylene) (PTFE) flow channel device where the SERS substrates were used as an electrode. Ulva density, determined in situ by SERS and ex situ by electron and fluorescence microscopy, decreased under successively increasing low negative potentials up to −1.0 V. The presence of damaged Ulva suggests that the applied potential led to spore rupture. At the highest negative applied potential (−1.0 V), microparticles containing copper, which is known for its antimicrobial properties, were associated with Ulva on the SERS substrate and the lowest Ulva density was observed. These findings indicate that (1) SERS can be employed to study biofilm formation on nanostructured metal surfaces and (2) applying low-voltage electric potentials may be used to control Ulva biofouling on SERS marine sensors

Association between genetic variant in hsa-miR-146a gene and prostate cancer progression: evidence from Serbian population

© 2014, Springer International Publishing Switzerland. Purpose: Two previous studies of association between rs2910164 in miR-146a gene and prostate cancer (PCa) risk have provided opposing results. Furthermore, no evidence of association of this SNP with standard prognostic parameters of PCa progression was obtained in mentioned studies. The main aim of this study was to evaluate the possible association between PCa onset and progression to a more aggressive form, since it has not been assessed in a population of European descent.Methods: In this study, 286 samples of peripheral blood were obtained from patients with PCa, while the control group comprised 199 volunteers derived from general population who gave samples of buccal swabs. For individuals diagnosed with PCa clinicopathological characteristics including serum prostate-specific antigen level at diagnosis, Gleason score (GS), and clinical stage were determined. Genotyping of rs2910164 was performed using Taqman®SNP Genotyping Assay. Analysis of SNP association was done using PLINK and SNPStats software.Results: rs2910164 showed no association with PCa risk. Nevertheless, heterozygous genotype was found to be associated with higher GS, as well as with the presence of distant metastases. rs2910164 was also shown to be associated with cancer aggressiveness (p = 0.0067; ORGC = 2.22, 95 %CI 1.24–3.97; ORCC = 0.47, 95 %CI 0.13–1.68).Conclusions: Our results show no evidence of association between rs2910164 and PCa risk in Serbian population. Conversely, this variant was found to be associated with PCa aggressiveness

Assessment of possible association between rs3787016 and prostate cancer risk in Serbian population

Recent study, which included meta-analysis of two genome-wide association studies (GWAS), followed by a replication, identified the association between single nucleotide polymorphism (SNP) rs3787016 at 19p13 and prostate cancer (PCa) risk. Considering possible genetic differences between populations, we conducted the study in order to evaluate the association of this polymorphism with prostate cancer risk in Serbian population. 261 samples of peripheral blood were obtained from the patients with PCa and 257 samples from patients with benign prostatic hyperplasia (BPH). 106 volunteers who gave samples of bucal swabs comprised the control group. For individuals diagnosed with PCa clinicopathological characteristics including serum prostate-specific antigen (PSA) level at diagnosis, Gleason score (GS) and clinical stage were determined. Genotypization of rs3787016 was performed by using Taqman(®) SNP Genotyping Assay. The differences in alelle and genotype frequencies between analyzed groups of subjects were performed by using PLINK, SPSS 17.0 for Windows and SNPStats statistical software. No significant association of rs3787016 with PCa risk was determined comparing allele and genotype frequencies among group of patients diagnosed with PCa and the control group, as well as among groups of patients with PCa and BPH. Also, no evidence of association of rs3787016 with PCa risk was shown using tests for association under dominant and recessive genetic models. SNP rs3787016 showed no significant association with standard prognostic parameters regarding PCa progression, nor with the risk of disease progression assessed according to two different risk classification systems