Multivariate statistical techniques for the assessment of seasonal variations in surface water quality of pasture ecosystems

This study investigates the applicability of multivariate statistical techniques including cluster analysis (CA), discriminant analysis (DA), and factor analysis (FA) for the assessment of seasonal variations in the surface water quality of tropical pastures. The study was carried out in the TPU catchment, Kuala Lumpur, Malaysia. The dataset consisted of 1-year monitoring of 14 parameters at six sampling sites. The CA yielded two groups of similarity between the sampling sites, i.e., less polluted (LP) and moderately polluted (MP) at temporal scale. Fecal coliform (FC), NO3, DO, and pH were significantly related to the stream grouping in the dry season, whereas NH3, BOD, Escherichia coli, and FC were significantly related to the stream grouping in the rainy season. The best predictors for distinguishing clusters in temporal scale were FC, NH3, and E. coli, respectively. FC, E. coli, and BOD with strong positive loadings were introduced as the first varifactors in the dry season which indicates the biological source of variability. EC with a strong positive loading and DO with a strong negative loading were introduced as the first varifactors in the rainy season, which represents the physiochemical source of variability. Multivariate statistical techniques were effective analytical techniques for classification and processing of large datasets of water quality and the identification of major sources of water pollution in tropical pastures

Grazing Effects on the Seed Pool of \u3cem\u3eStipa Krylovii\u3c/em\u3e and Its Genetic Diversity in Relationship to the Plant Population on a Typical Steppe Community in Inner Mongolia

Stipa krylovii is an important tufted forage species on the typical steppe in Inner Mongolia and is sensitive to heavy grazing pressure. Vegetative recovery of plant density is dependent on the seed bank, which is a genetic reservoir that supports the vegetative expression of the species thus enhancing its resilience (McCue and Holtsford 1998). The ability of the seed bank to support the Stipa krylovii population is dependent on its size and genetic diversity. Therefore, we conducted a study to determine the effects of heavy grazing pressure on its seed reserves and examine its genetic diversity in relation to surviving plants

Grazing Impacts on the Production Stability of Fescue Prairie

The fescue prairie in southwestern Alberta is highly productive but susceptible to grazing during the growing season. This study examined how production stability is affected by grazing impact. It was initiated in 1949 using four fixed stocking rates (1.2, 1.6, 2.4, 4.8 animal-unitmonths ha-1). Forage and livestock production were estimated over 4 or 15 years, respectively. The coefficient of variation (CV) for either primary or secondary production was constant over all rates except for the very heavily stocked paddock. Forage production, and therefore livestock production, in that paddock was dependent on precipitation during the growing season. Heavy grazing pressure resulted in lost management opportunities and jeopardized sustainable production

Grazing Effects on Genetic Diversity of \u3cem\u3eFestuca Campestris\u3c/em\u3e Rydb. And \u3cem\u3eStipa Grandis\u3c/em\u3e L. On the Native Grasslands in Canada and China, Respectively

Genetic drift or selectively neutral mutation in finite populations may result in genetic diversity within a natural population (Kimura, 1986). Genetic diversity influences the resilience of a species to survive perturbations or adapt to changes in its environment. Grazing by livestock may affect genetic diversity by exerting selection pressure on grazing sensitive species. In this study, we examine the effects of heavy sustained grazing pressure on the genetic diversity of Festuca campestris Rydb. and Stipa grandis L. These species are found on the Canadian Plains and the steppes of Inner Mongolia, respectively. Each is an important forage species that dominates their respective grasslands but decline readily when subjected to heavy grazing pressure

Применение информационных технологий и этические нюансы в управлении массовым сознанием

This paper discusses the current value of information technology in our lives, as well as their application to control the mass consciousness

Acidic microenvironment plays a key role in human melanoma progression through a sustained exosome mediated transfer of clinically relevant metastatic molecules

Background: Microenvironment cues involved in melanoma progression are largely unknown. Melanoma is highly influenced in its aggressive phenotype by the changes it determinates in its microenvironment, such as pH decrease, in turn influencing cancer cell invasiveness, progression and tissue remodelling through an abundant secretion of exosomes, dictating cancer strategy to the whole host. A role of exosomes in driving melanoma progression under microenvironmental acidity was never described. Methods: We studied four differently staged human melanoma lines, reflecting melanoma progression, under microenvironmental acidic pHs pressure ranging between pH 6.0-6.7. To estimate exosome secretion as a function of tumor stage and environmental pH, we applied a technique to generate native fluorescent exosomes characterized by vesicles integrity, size, density, markers expression, and quantifiable by direct FACS analysis. Functional roles of exosomes were tested in migration and invasion tests. Then we performed a comparative proteomic analysis of acid versus control exosomes to elucidate a specific signature involved in melanoma progression. Results: We found that metastatic melanoma secretes a higher exosome amount than primary melanoma, and that acidic pH increases exosome secretion when melanoma is in an intermediate stage, i.e. metastatic non-invasive. We were thus able to show that acidic pH influences the intercellular cross-talk mediated by exosomes. In fact when exposed to exosomes produced in an acidic medium, pH naïve melanoma cells acquire migratory and invasive capacities likely due to transfer of metastatic exosomal proteins, favoring cell motility and angiogenesis. A Prognoscan-based meta-analysis study of proteins enriched in acidic exosomes, identified 11 genes (HRAS, GANAB, CFL2, HSP90B1, HSP90AB1, GSN, HSPA1L, NRAS, HSPA5, TIMP3, HYOU1), significantly correlating with poor prognosis, whose high expression was in part confirmed in bioptic samples of lymph node metastases. Conclusions: A crucial step of melanoma progression does occur at melanoma intermediate -stage, when extracellular acidic pH induces an abundant release and intra-tumoral uptake of exosomes. Such exosomes are endowed with pro-invasive molecules of clinical relevance, which may provide a signature of melanoma advancement

Strategies on Poisonous Plants Problem in China

Poisonous plants are widely distributed on large areas of native grasslands of China, causing livestock poisoning and grassland degradation, which severely impacts the development of animal husbandry. Of the almost 300 poisonous species that are responsible for livestock losses in China, locoweed, drunken horse grass and Langdu cause the greatest impact. Many strategies have been developed to minimise the impact of poisonous plants including the treatment of livestock that have been poisoned, controlling poisonous plants and managing livestock grazing. Both physical and chemical traditional methods are still used to eliminate poisonous plants while biological control using specific insects may eventually be used to control certain species. According to a grassland law, grazing systems (rotational, rest and forbidden grazing) may be applied on dense stands of poisonous plants