Prevalence of emergency contraceptive pill use among Spanish adolescent girls and their family and psychological profiles

The Author(s). 2018 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.Background:Adolescent girls’ family context and psychological characteristics play important roles in their sexual behavior, including the use of the emergency contraceptive pill (ECP). This study aims to (1) determine the prevalence of ECP use among girls who have had sexual intercourse and (2) comparatively analyze their family and psychological profiles according to whether they have used ECPs. Methods:The sample of 1735 Spanish girls aged 15 to 18 came from a representative sample of the 2014 edition of the Health Behaviour in School-aged Children (HBSC) study. Of this sample, 398 girls had sexual intercourse and reported their ECP use. Data collection for the HBSC study was performed through an online questionnaire to which adolescents responded anonymously in school. Data analyses were descriptive and bivariate and were performed with the statistical program IBM SPSS Statistics 23.Results:The results demonstrated that 30.65% of girls who had sexual intercourse used ECPs. Noticeable differences in paternal knowledge and communication with the father were observed between girls who used the ECP at least once and those who did not use it. In contrast, differences between girls who used the ECP once and those who used it twice or more were pronounced with regard to parental knowledge, communication with parents, maternal affection,life satisfaction, sense of coherence and depression. Conclusions:This work demonstrates a high prevalence of ECP use and a more positive family and psychological profile for girls who used ECP once compared with those who used it twice or more.Peer reviewedFinal Published versio

Detection of leptospiral antibodies and DNA in freshwater fish

Leptospirosis is an important zoonotic disease that is maintained in populations due to chronic kidney infection of reservoir mammals. Previous work from our lab has identified rodents, voles, shrews, chipmunks and several species of amphibians and reptiles as hosts of Leptospira spp. in the Cumberland Gap Region of Kentucky, Tennessee, and Virginia. The aim of this study was to determine if fish contribute to the maintenance of the pathogen in the aquatic environment. Fish (n = 238), belonging to 19 genera, were collected from seven different locations in the Powell River in East Tennessee. Fish kidneys were harvested and screened for leptospiral DNA using a TaqMan quantitative polymerase chain reaction (qPCR) assay that targets pathogenic Leptospira spp. Blood samples were collected for measuring leptospiral antibodies using microscopic agglutination test (MAT). Of the 238 fish screened, 11 were positive by either qPCR or MAT (4.62%; 95% CI: 2.33–8.12). Of these 3 (3/238; 1.26%; 95% CI: 0.26–3.64) were positive by qPCR and 8 (8/237; 3.38%; 95% CI: 1.47–6.54) were found to have antibodies to at least one leptospiral serovar by MAT. This is the first report of leptospiral DNA detection in fish kidneys, providing insights on the potential role of fish in the epidemiology of leptospirosis in the region

Double-Bond Requirement in a Fatty Acid Desaturase Mutant of <i>Saccharomyces cerevisiae</i>

A fatty acid Δ 9 -desaturase mutant of yeast was analyzed to establish the specificity of the fatty acid structural requirement. Several double-bond-containing and substituted fatty acids were tested. It was concluded that the presence of a Δ 9 cis double bond is necessary for growth. The need for a specific chain length or a specific number of double bonds, or for both, is more flexible. Tracer-containing 16:1Δ 9 cis , 18:1Δ 9 cis , 18:2Δ 9,12 cis,cis and 18:3Δ 9,12,15 all cis revealed that each of these growth-supporting components, once taken up, was not converted into other fatty acids. Concentration effects on doubling time were also considered. </jats:p

Unique C1 inhibitor dysfunction in a kindred without angioedema. I. A mutant C1 INH that inhibits C1-s but not C1-r.

Abstract We have described hereditary incomplete deficiency of the fourth component of complement (C4) in 10 members of a large kindred. C4 deficiency in this kindred is not linked to C4 loci in the HLA region. C4 synthesis is decreased, and C4 catabolism is normal in kindred members with low serum C4 levels. We have discovered a uniquely dysfunctional C1 inhibitor in all C4-deficient members of this kindred. C1 inhibitor dysfunction is revealed by incubating sera of affected members with EDTA, which destroys all C4 activity in these sera, but not in normal sera or sera from individuals with partial C4 deficiencies. The M(r) of C1 inhibitor purified from affected members is normal, but approximately 50% of this C1 inhibitor resists cleavage by trypsin (0.14 microM) at arg444, suggesting a substitution at this position. Moderate increases in trypsin, however, result in cleavage of the resistant molecules, which would not be expected if arg444 were the site of the mutation. All molecules in C1 inhibitor purified from affected members' plasma bind to activated C1s (C1-s), but approximately 50% of molecules in these preparations do not bind to activated C1r (C1r). These findings show that affected kindred members have a unique mutation in C1 inhibitor. The mutant C1 inhibitor does not prevent the activation of C1s by C1-r when serum Ca2+ is chelated by EDTA, but its inhibition of C1-s is normal in vivo, as shown by normal C2 levels, normal C4 catabolism, and absence of angioedema in C4-deficient members. The nature of the mutation, its selective failure to inhibit C1-r, and its relationship to decreased C4 synthesis remain to be defined.</jats:p

Comparison of Juvenile Feed Protocols on Growth and Spawning in Zebrafish

Over the past 2 decades, zebrafish, Danio rerio, have become a mainstream laboratory animal model, yet zebrafish husbandry practices remain far from standardized. Feeding protocols play a critical role in the health, wellbeing, and productivity of zebrafish laboratories, yet they vary significantly between facilities. In this study, we compared our current feeding protocol for juvenile zebrafish (30 dpf to 75 dpf), a 3:1mixture of fish flake and freeze-dried krill fed twice per day with live artemia twice per day (FKA), to a diet of Gemma Micro 300 fed once per day with live artemia once per day (GMA). Our results showed that juvenile EK wild-type zebrafish fed GMA were longer and heavier than juveniles fed FKA. As compared with FKA-fed juveniles, fish fed GMA as juveniles showed better reproductive performance as measured by spawning success, fertilization rate, and clutch size. As adults, fish from both feeding protocols were acclimated to our standard adult feeding protocol, and the long-term effects of juvenile diet were assessed. At 2 y of age, the groups showed no difference in mortality or fecundity. Reproductive performance is a crucial aspect of zebrafish research, as much of the research focuses on the developing embryo. Here we show that switching juvenile zebrafish from a mixture of flake and krill to Gemma Micro 300 improves reproductive performance, even with fewer feedings of live artemia, thus simplifying husbandry practices.</jats:p

Analysis of anti-C1q autoantibodies by western blot

Anti-C1q autoantibodies may be found in many conditions, most commonly in systemic lupus erythematosus (SLE) and hypocomplementemic urticarial vasculitis syndrome (HUVS), and are diagnostic markers as well as disease activity markers in lupus nephritis. Sera from patients with SLE and HUVS show partly distinct autoantibody reactivities to separated protein chains B and C of the first component of complement, C1q. These different binding specificities can be detected by Western blot analysis of the autoantibodies under reducing conditions. Results may help clinicians to differentiate between SLE and HUVS

Localization of diphtheria toxin nuclease activity to fragment A.

We describe a series of experiments that aimed to establish whether nuclease activity is actually associated with diphtheria toxin (DTx) and its A subunit (DTA), as we originally reported (M. P. Chang, R. L. Baldwin, C. Bruce, and B. J. Wisnieski, Science 246:1165-1168, 1989). Here we show that (i) trypsinization of DTx does indeed produce nucleolytically active DTA, (ii) reduction of electroeluted, unreduced, cleaved DTx (58 kDa) yields nuclease-active DTA (24 kDa), and (iii) fractionation of DTx and DTA by anion-exchange chromatography leads to coelution of nuclease activity with both forms of the toxin, even though each form elutes at a distinct salt concentration. In addition, we show that Escherichia coli-derived DTA also expresses nuclease activity. These studies confirm our initial assertion that the nuclease activity observed in DTx preparations is intrinsic to the DTA portion of DTx