Study of permeability characteristics of membranes Quarterly reports, 9 Nov. 1967 - 9 Apr. 1968

Permeability characteristics and transport properties of membranes for salt water conversion, and experiment design

Study of permeability characteristics of membranes Quarterly report, 9 Feb. - 9 May 1969

Permeability characteristics of membrane

Study of permeability characteristics of membranes Quarterly report, 9 May - 9 Aug. 1969

Demineralizing gear pump system with mixed bed ion exchange columns for salt and volume transport experimen

Study of permeability characteristics of membranes Quarterly progress report, 9 Apr. - 9 Aug. 1968

Electrochemical cell constructed to measure membrane transport propertie

Rotation and Macroturbulence in Metal-poor Field Red Giant and Red Horizontal Branch Stars

We report the results for rotational velocities, Vrot sin i, and macroturbulence dispersion, zeta(RT), for 12 metal-poor field red giant branch stars and 7 metal-poor field red horizontal branch stars. The results are based on Fourier transform analyses of absorption line profiles from high-resolution (R ~ 120,000), high-S/N (~ 215 per pixel) spectra obtained with the Gecko spectrograph at CFHT. We find that the zeta(RT) values for the metal-poor RGB stars are very similar to those for metal-rich disk giants studied earlier by Gray and his collaborators. Six of the RGB stars have small rotational values, less than 2.0 km/sec, while five show significant rotation, over 3 km/sec. The fraction of rapidly rotating RHB stars is somewhat lower than found among BHB stars. We devise two empirical methods to translate the line-broadening results obtained by Carney et al. (2003, 2008) into Vrot sin i for all the RGB and RHB stars they studied. Binning the RGB stars by luminosity, we find that most metal-poor field RGB stars show no detectable sign, on average, of rotation. However, the most luminous stars, with M(V) <= -1.5, do show net rotation, with mean values of 2 to 4 km/sec, depending on the algorithm employed, and these stars also show signs of radial velocity jitter and mass loss.Comment: accepted for publication in the Astronomical Journa

Biosynthesis of Mitochondrial Porin and Insertion into the Outer Mitochondrial Membrane of Neuruspora crassa

Mitochondrial porin, the major protein of the outer mitochondrial membrane is synthesized by free cytoplasmic polysomes. The apparent molecular weight of the porin synthesized in homologous or heterologous cell-free systems is the same as that of the mature porin. Transfer in vitro of mitochondrial porin from the cytosolic fraction into the outer membrane of mitochondria could be demonstrated. Before membrane insertion, mitochondrial porin is highly sensitive to added proteinase; afterwards it is strongly protected. Binding of the precursor form to mitochondria occurs at 4°C and appears to precede insertion into the membrane. Unlike transfer of many precursor proteins into or across the inner mitochondrial membrane, assembly of the porin is not dependent on an electrical potential across the inner membrane

ENIGMA and global neuroscience: A decade of large-scale studies of the brain in health and disease across more than 40 countries

This review summarizes the last decade of work by the ENIGMA (Enhancing NeuroImaging Genetics through Meta Analysis) Consortium, a global alliance of over 1400 scientists across 43 countries, studying the human brain in health and disease. Building on large-scale genetic studies that discovered the first robustly replicated genetic loci associated with brain metrics, ENIGMA has diversified into over 50 working groups (WGs), pooling worldwide data and expertise to answer fundamental questions in neuroscience, psychiatry, neurology, and genetics. Most ENIGMA WGs focus on specific psychiatric and neurological conditions, other WGs study normal variation due to sex and gender differences, or development and aging; still other WGs develop methodological pipelines and tools to facilitate harmonized analyses of "big data" (i.e., genetic and epigenetic data, multimodal MRI, and electroencephalography data). These international efforts have yielded the largest neuroimaging studies to date in schizophrenia, bipolar disorder, major depressive disorder, post-traumatic stress disorder, substance use disorders, obsessive-compulsive disorder, attention-deficit/hyperactivity disorder, autism spectrum disorders, epilepsy, and 22q11.2 deletion syndrome. More recent ENIGMA WGs have formed to study anxiety disorders, suicidal thoughts and behavior, sleep and insomnia, eating disorders, irritability, brain injury, antisocial personality and conduct disorder, and dissociative identity disorder. Here, we summarize the first decade of ENIGMA's activities and ongoing projects, and describe the successes and challenges encountered along the way. We highlight the advantages of collaborative large-scale coordinated data analyses for testing reproducibility and robustness of findings, offering the opportunity to identify brain systems involved in clinical syndromes across diverse samples and associated genetic, environmental, demographic, cognitive, and psychosocial factors

3D osteocyte lacunar morphometry of human bone biopsies with high resolution microCT : from monoclonal gammopathy to newly diagnosed multiple myeloma

Osteocytes are bone-embedded cells connected via canaliculi and they regulate the bone resorption/formation balance. Osteocyte function is altered in skeletal disorders including cancer. Multiple myeloma (MM) is a hematological malignancy, whereby plasma cells disrupt the bone homeostasis and induce excessive resorption of the mineralized extracellular matrix (ECM), as evidenced by clinical computed tomography (CT). However, morphometric analyses of the mineralized ECM and bone microporosity are not yet performed in patients with MM, nor in the precursor conditions of monoclonal gammopathy of undetermined significance (MGUS) and smoldering MM (SMM). Here, we characterize changes in 3D osteocyte lacunar morphology in bone biopsy samples obtained from patients with MGUS, SMM and newly diagnosed MM, using high resolution microCT. In the small lacunae 20-900 um3 range, we found a trend for lower lacunar density and increasing 50% cutoff of the lacunar volume cumulative distribution for diseased samples. In the larger lacunae 900-3000 um3 range, we found significantly higher lacunar density and microporosity in the MM group compared to the MGUS/SMM group. Regarding the shape distribution, the MGUS/SMM group showed a trend for flatter, more elongated and anisotropic osteocyte lacunae compared to the control group, though the differences were not statistically significant. Altogether, our findings suggest that osteocytes in human MM bone disease undergo changes in their lacunae morphology, which could be an indicator for impaired bone quality. Future studies are needed to show whether these are consistent within comparable anatomical sites and could serve as a diagnostic or prognostic marker