ФОТОДИНАМИЧЕСКАЯ ТЕРАПИЯ ПЛОСКОКЛЕТОЧНОГО РАКА КОЖИ ЩЕКИ (КЛИНИЧЕСКОЕ НАБЛЮДЕНИЕ)

The  results  of the  clinical observation  of a female  patient  with squamous cell carcinoma  of the  cheek  skin treated  by the  method of photodynamic therapy (PDT) using the photosensitizer of fotoditazin are represented. The patient  had single course of PDT. Fotoditazin was injected in a dose of 0.8 mg/kg of body weight as a single intravenous  infusion in 0.9% sodium chloride. The irradiation was performed  once, 2.5 h after injection of the photosensitizer (power density of laser irradiation – 200-300 mW/cm2, light dose of a single session of 300-350 J/ cm2). There were no side effects after photosensitizer injection and after irradiation. The result of the treatment was defined as partial tumor regression. For nowadays the follow-up period is 2 months. The repeated course of PDT is scheduled.Приведены результаты клинического наблюдения за пациенткой с плоскоклеточным раком кожи щеки, пролеченной методом фотодинамической терапии с фотосенсибилизатором фотодитазин. Пациентке проведен  однократный  курс фотодинамической  терапии. Фотодитазин вводили в дозе 0,8 мг/кг массы тела путем однократной  внутривенной инфузии в 0,9%-ом растворе  натрия хлорида. Облучение проводили  однократно, через 2,5 ч после введения  фотосенсибилизатора (плотность мощности лазерного  излучения – 200-300 мВт/см2, световая доза одного сеанса – 300-350 Дж/см2. Побочных эффектов после введения фотосенсибилизатора и проведения облучения не зарегистрировано. Результат лечения оценен как частичная регрессия опухоли. В настоящее время пациентка находится под динамическим наблюдением, срок наблюдения 2 мес. Планируется проведение повторного курса ФДТ

Исследование влияния вида обработки на прочность монокристаллических пластин нелегированного антимонида индия

The method of plane-transverse bending was used to measure the strength of thin single-crystal plates of undoped InSb with a crystallographic orientation of (100). It was found that the strength of the plates (thickness ≤ 800 μm) depends on their processing. Using a full processing cycle (grinding and chemical polishing) allows to increase the strength of InSb plates by 2 times (from 3.0 to 6.4 kg/mm2). It is shown that the dependence of strength on processing for wafers with (100) orientation is similar to this dependence for wafers (111), while the strength of wafers (111) is 2 times higher. The contact profilometry method was used to measure the roughness of thin plates, which also passed successive processing steps. It was found that during a full cycle of processing, the roughness of InSb plates decreases (Ra from 0.6 to 0.04 μm), leading to a general smoothing of the surface roughness. The strength and roughness of the (100) InSb and GaAs wafers are compared. It was found that the strength of GaAs cut wafers is 2 times higher than the strength of InSb cut wafers and slightly increases after a full cycle of their processing. It was shown that the roughness of GaAs and InSb plates after a full cycle of surface treatment is significantly reduced: 10 times for InSb due to overall surface leveling and 3 times for GaAs (Rz from 2.4 to 0.8 μm) due to a decrease in the peak component. Conducting a full cycle of processing InSb plates can increase their strength by removing broken layers by sequential operations and reducing the risk of mechanical damage.Методом плоско-поперечного изгиба проведены измерения прочности тонких монокристаллических пластин нелегированного InSb с кристаллографической ориентацией (100). Установлено, что прочность пластин (толщиной ≤ 800 мкм) зависит от их обработки. Использование полного цикла обработки (шлифовки и химической полировки) позволяет увеличить прочность пластин InSb в 2 раза (от 3,0 до 6,4 кг/мм2). Показано, что зависимость прочности от обработки для пластин с ориентацией (100) аналогична этой зависимости для пластин (111), при этом величина прочности пластин (111) в 2 раза выше. Методом контактной профилометрии измерена шероховатость тонких пластин, также прошедших последовательные этапы обработки. Установлено, что при проведении полного цикла обработки шероховатость пластин InSb уменьшается (Ra от 0,6 до 0,04 мкм), приводя к общему выравниванию шероховатости на поверхности. Проведено сравнение прочности и шероховатости пластин (100) InSb и GaAs. Установлено, что прочность резаных пластин GaAs в 2 раза выше прочности резаных пластин InSb и незначительно увеличивается после полного цикла их обработки. Показано, что шероховатость пластин GaAs и InSb после полного цикла обработки поверхности значительно уменьшается: в 10 раз для InSb за счет общего выравнивания поверхности и в 3 раза для GaAs (Rz от 2,4 до 0,8 мкм) за счет снижения пиковой составляющей. Проведение полного цикла обработки пластин InSb позволяет повысить их прочность, удаляя нарушенные слои последовательными операциями и снижая риск развития механических повреждений

Vaccination with M2e-Based Multiple Antigenic Peptides: Characterization of the B Cell Response and Protection Efficacy in Inbred and Outbred Mice

The extracellular domain of the influenza A virus protein matrix protein 2 (M2e) is remarkably conserved between various human isolates and thus is a viable target antigen for a universal influenza vaccine. With the goal of inducing protection in multiple mouse haplotypes, M2e-based multiple antigenic peptides (M2e-MAP) were synthesized to contain promiscuous T helper determinants from the Plasmodium falciparum circumsporozoite protein, the hepatitis B virus antigen and the influenza virus hemagglutinin. Here, we investigated the nature of the M2e-MAP-induced B cell response in terms of the distribution of antibody (Ab) secreting cells (ASCs) and Ab isotypes, and tested the protective efficacy in various mouse strains.Immunization of BALB/c mice with M2e-MAPs together with potent adjuvants, CpG 1826 oligonucleotides (ODN) and cholera toxin (CT) elicited high M2e-specific serum Ab titers that protected mice against viral challenge. Subcutaneous (s.c.) and intranasal (i.n.) delivery of M2e-MAPs resulted in the induction of IgG in serum and airway secretions, however only i.n. immunization induced anti-M2e IgA ASCs locally in the lungs, correlating with M2-specific IgA in the bronchio-alveolar lavage (BAL). Interestingly, both routes of vaccination resulted in equal protection against viral challenge. Moreover, M2e-MAPs induced cross-reactive and protective responses to diverse M2e peptides and variant influenza viruses. However, in contrast to BALB/c mice, immunization of other inbred and outbred mouse strains did not induce protective Abs. This correlated with a defect in T cell but not B cell responsiveness to the M2e-MAPs.Anti-M2e Abs induced by M2e-MAPs are highly cross-reactive and can mediate protection to variant viruses. Although synthetic MAPs are promising designs for vaccines, future constructs will need to be optimized for use in the genetically heterogeneous human population

Epitope mapping of avian influenza m2e protein: different species recognise various epitopes

Published: June 30, 2016A common approach for developing diagnostic tests for influenza virus detection is the use of mouse or rabbit monoclonal and/or polyclonal antibodies against a target antigen of the virus. However, comparative mapping of the target antigen using antibodies from different animal sources has not been evaluated before. This is important because identification of antigenic determinants of the target antigen in different species plays a central role to ensure the efficiency of a diagnostic test, such as competitive ELISA or immunohistochemistry-based tests. Interest in the matrix 2 ectodomain (M2e) protein of avian influenza virus (AIV) as a candidate for a universal vaccine and also as a marker for detection of virus infection in vaccinated animals (DIVA) is the rationale for the selection of this protein for comparative mapping evaluation. This study aimed to map the epitopes of the M2e protein of avian influenza virus H5N1 using chicken, mouse and rabbit monoclonal or monospecific antibodies. Our findings revealed that rabbit antibodies (rAbs) recognized epitope 6EVETPTRN13 of the M2e, located at the N-terminal of the protein, while mouse (mAb) and chicken antibodies (cAbs) recognized epitope 10PTRNEWECK18, located at the centre region of the protein. The findings highlighted the difference between the M2e antigenic determinants recognized by different species that emphasized the importance of comparative mapping of antibody reactivity from different animals to the same antigen, especially in the case of multi-host infectious agents such as influenza. The findings are of importance for antigenic mapping, as well as diagnostic test and vaccine development.Noor Haliza Hasan, Esmaeil Ebrahimie, Jagoda Ignjatovic, Simson Tarigan, Anne Peaston, Farhid Hemmatzade

Ecological State of Roadside Soils of the Ussuriysk - Pogranichny Highway on the Border Territory

The main physical and chemical properties and the content of heavy metals in the topsoils of roadside’s were determined. Most soils are heavy loams, low-humus, slightly acidic and neutral. Motor vehicle emissions are the main source of contamination of roadside soils with Co, Cu, Cr, Ni, average concentrations of which are 1.3-4.8 times higher than the background level. The highest values of the NPI pollution index were found in the soils of a flat area with a high concentration of heavy-duty vehicles operating at idle. There are significant differences in determining the degree of soil contamination with using various geochemical indices.</jats:p

PREDICTORS OF ADVERSE LEFT VENTRICULAR REMODELING IN PATIENTS AFTER MYOCARDIAL INFARCTION

Functional recovery or irreversible negative remodeling of injured myocardial segments has different prognostic implication in patients with myocardial infarction (MI) undergoing coronary percutaneous intervention (PCI). Increased pulse wave velocity (PWV), a non-invasive index of arterial stiffness, is one of the important risk factors of cardiovascular events in different clinical conditions. The aim of the study was to assess the relationship between PWV and changes of left ventricular (LV) systolic function in patients with acute MI. Adverse cardiac remodeling was revealed in 72% of patients and they more often had non-recovery of left ventricular longitudinal function. Higher baseline PWV is associated with less effective recovery of LV function

Polymorphisms in SSR-loci associated with E genes in soybean mutant lines offer perspective for breeding

Aim. To analyse genetic diversity in 10 new soybean lines created by using the chemical mutagens D-6, DMSSO-11, DMSSO-12, DMSNPIR-11, DUDMS12, D12DMC-11B obtained from four cultivars Femida, Oksana, Podils’ka 416, Zolotysta. The microsatellite (MS) markers Satt100, Satt229, Satt319, Satt354, Satt365, Sat_038 were used. These markers are linked with genes, which determine sensitivity of soybean plants to photoperiod and time to maturation. Methods of DNA extraction, PCR, MS-analysis, fi eld trial, one-way analysis of variance (ANOVA) have been applied. Results. Parental cultivars, mutant lines and control genotypes were characterized by alleles of microsatellite loci, 25 alleles of 6 microsatellite loci were detected. Signifi cant differences between investigated lines were detected in three year fi eld trials for traits − days to maturation (DTM) and length of the vegetative period (LV). We have revealed effects of the factor «Alleles of MS-locus», so alleles of Satt100 locus affected all traits except DTF (days to fl owering); alleles of Satt319 and Satt354 affected DTM and LV; Sat_038 affected DTF and S-F (duration of the period shoots-fl owering). Lines with alleles 167 bp at Satt100 and 175 bp at Satt319 loci (that marks dominant E7) were shown to have a longer vegetation period and later maturity, than other. The lines with allele 247 bp at Sat_038 fl owered earlier, than lines with a 245 bp allele, and the lines with allele 232 bp at Satt354 reached maturity later, than lines with other alleles at this locus. Conclusions. We have found that applied mutagens induce changes in the soybean genome and by using these mutagens it is possible to effectively increase genetic diversity in loci associated with genes/loci that determine time of maturity and/or photoperiod sensitivity of soybean, enabling to obtain soybean cultivars with different terms of maturity and yield. The microsatellite markers, particularly Sat_038, Satt100, Satt319 and Satt354 that were applied in our study are considered to be useful tools for marker assisted breeding of soybean cultivars with programmed time of development. We did not observe signifi cant effects of «Alleles of MS-locus Satt229» that is known to be linked with E3 on the investigated agronomical traits. For soybean genotypes with the E7 allele the DTF was longer for 3-9 days and LV for 10-11 days. In lines with an allele of 175 bp at locus Satt319 the S-F period was 6-9 days shorter</jats:p

PHOTODYNAMIC THERAPY FOR SQUAMOUS CELL SKIN CANCER OF THE CHEEK (CASE REPORT)

The  results  of the  clinical observation  of a female  patient  with squamous cell carcinoma  of the  cheek  skin treated  by the  method of photodynamic therapy (PDT) using the photosensitizer of fotoditazin are represented. The patient  had single course of PDT. Fotoditazin was injected in a dose of 0.8 mg/kg of body weight as a single intravenous  infusion in 0.9% sodium chloride. The irradiation was performed  once, 2.5 h after injection of the photosensitizer (power density of laser irradiation – 200-300 mW/cm2, light dose of a single session of 300-350 J/ cm2). There were no side effects after photosensitizer injection and after irradiation. The result of the treatment was defined as partial tumor regression. For nowadays the follow-up period is 2 months. The repeated course of PDT is scheduled