A shared role for RBF1 and dCAP-D3 in the regulation of transcription with consequences for innate immunity

Previously, we discovered a conserved interaction between RB proteins and the Condensin II protein CAP-D3 that is important for ensuring uniform chromatin condensation during mitotic prophase. The Drosophila melanogaster homologs RBF1 and dCAP-D3 co-localize on non-dividing polytene chromatin, suggesting the existence of a shared, non-mitotic role for these two proteins. Here, we show that the absence of RBF1 and dCAP-D3 alters the expression of many of the same genes in larvae and adult flies. Strikingly, most of the genes affected by the loss of RBF1 and dCAP-D3 are not classic cell cycle genes but are developmentally regulated genes with tissue-specific functions and these genes tend to be located in gene clusters. Our data reveal that RBF1 and dCAP-D3 are needed in fat body cells to activate transcription of clusters of antimicrobial peptide (AMP) genes. AMPs are important for innate immunity, and loss of either dCAP-D3 or RBF1 regulation results in a decrease in the ability to clear bacteria. Interestingly, in the adult fat body, RBF1 and dCAP-D3 bind to regions flanking an AMP gene cluster both prior to and following bacterial infection. These results describe a novel, non-mitotic role for the RBF1 and dCAP-D3 proteins in activation of the Drosophila immune system and suggest dCAP-D3 has an important role at specific subsets of RBF1-dependent genes

Form management

This paper consists of three interrelated parts. In the first part forms are intoduced as an abstraction and generalization of business paper forms. A set of facilities for the manipulation of forms and their contents is outlined. Forms can be created, stored, found, viewed in different media, mailed, and located by office workers. Data on forms can also be processed in a completely integrated way. The facilities are discussed both abstractly and in relation to a prototype system. In the second part a facility is outlined for the specification and implementation of automatic form procedures. These procedures specify actions on forms which are triggered automatically when certain preconditions are met. The preconditions, actions, and specification method are based on forms. The discussion is centered on our implementation of such a specification framework. Finally, in the third part, techniques for the analysis of office flow are specified. An algorithm is outlined for the categorization of forms into classes depending on the local routing and actions on the forms. In this way, we can obtain the paths that forms take and analyze the system for correctness and loading characteristics. </jats:p