Anaerobic Carbon Monoxide Dehydrogenase Diversity in the Homoacetogenic Hindgut Microbial Communities of Lower Termites and the Wood Roach

Anaerobic carbon monoxide dehydrogenase (CODH) is a key enzyme in the Wood-Ljungdahl (acetyl-CoA) pathway for acetogenesis performed by homoacetogenic bacteria. Acetate generated by gut bacteria via the acetyl-CoA pathway provides considerable nutrition to wood-feeding dictyopteran insects making CODH important to the obligate mutualism occurring between termites and their hindgut microbiota. To investigate CODH diversity in insect gut communities, we developed the first degenerate primers designed to amplify cooS genes, which encode the catalytic (β) subunit of anaerobic CODH enzyme complexes. These primers target over 68 million combinations of potential forward and reverse cooS primer-binding sequences. We used the primers to identify cooS genes in bacterial isolates from the hindgut of a phylogenetically lower termite and to sample cooS diversity present in a variety of insect hindgut microbial communities including those of three phylogenetically-lower termites, Zootermopsis nevadensis, Reticulitermes hesperus, and Incisitermes minor, a wood-feeding cockroach, Cryptocercus punctulatus, and an omnivorous cockroach, Periplaneta americana. In total, we sequenced and analyzed 151 different cooS genes. These genes encode proteins that group within one of three highly divergent CODH phylogenetic clades. Each insect gut community contained CODH variants from all three of these clades. The patterns of CODH diversity in these communities likely reflect differences in enzyme or physiological function, and suggest that a diversity of microbial species participate in homoacetogenesis in these communities

Convergence to equilibrium for the discrete coagulation-fragmentation equations with detailed balance

Under the condition of detailed balance and some additional restrictions on the size of the coefficients, we identify the equilibrium distribution to which solutions of the discrete coagulation-fragmentation system of equations converge for large times, thus showing that there is a critical mass which marks a change in the behavior of the solutions. This was previously known only for particular cases as the generalized Becker-D\"oring equations. Our proof is based on an inequality between the entropy and the entropy production which also gives some information on the rate of convergence to equilibrium for solutions under the critical mass.Comment: 28 page

Four applications of embodied cognition

This article presents the views of four sets of authors, each taking concepts of embodied cognition into problem spaces where the new paradigm can be applied. The first considers consequences of embodied cognition on the legal system. The second explores how embodied cognition can change how we interpret and interact with art and literature. The third examines how we move through archi- tectural spaces from an embodied cognition perspective. And the fourth addresses how music cogni- tion is influenced by the approach. Each contribution is brief. They are meant to suggest the potential reach of embodied cognition, increase the visibility of applications, and inspire potential avenues for research

Probable detection of starlight reflected from the giant exoplanet orbiting tau Bootis

Giant planets orbiting stars other than the Sun are clearly detectable through precise radial-velocity measurements of the orbital reflex motion of the parent star. In the four years since the discovery of the companion to the star 51 Peg, similar low-amplitude ``Doppler star wobbles'' have revealed the presence of some 20 planets orbiting nearby solar-type stars. Several of these newly-discovered planets are very close to their parent stars, in orbits with periods of only a few days. Being an indirect technique, however, the reflex-velocity method has little to say about the sizes or compositions of the planets, and can only place lower limits on their masses. Here we report the use of high-resolution optical spectroscopy to achieve a probable detection of the Doppler-shifted signature of starlight reflected from one of these objects, the giant exoplanet orbiting the star tau Bootis. Our data give the planet's orbital inclination i=29 degrees, indicating that its mass is some 8 times that of Jupiter, and suggest strongly that the planet has the size and reflectivity expected for a gas-giant planet.Comment: 15 pages, 4 figures. (Fig 1 and equation for epsilon on p1 para 2 revised; changed from double to single spacing

Epidemiological associations between brachycephaly and upper respiratory tract disorders in dogs attending veterinary practices in England

Background: Brachycephalic dog breeds are increasingly common. Canine brachycephaly has been associated with upper respiratory tract (URT) disorders but reliable prevalence data remain lacking. Using primary-care veterinary clinical data, this study aimed to report the prevalence and breed-type risk factors for URT disorders in dogs. Results: The sampling frame included 170,812 dogs attending 96 primary-care veterinary clinics participating within the VetCompass Programme. Two hundred dogs were randomly selected from each of three extreme brachycephalic breed types (Bulldog, French Bulldog and Pug) and three common small-to medium sized breed types (moderate brachycephalic: Yorkshire Terrier and non-brachycephalic: Border Terrier and West Highland White Terrier). Information on all URT disorders recorded was extracted from individual patient records. Disorder prevalence was compared between groups using the chi-squared test or Fisher’s test, as appropriate. Risk factor analysis used multivariable logistic regression modelling. During the study, 83 (6.9 %) study dogs died. Extreme brachycephalic dogs (median longevity: 8.6 years, IQR: 2.4-10.8) were significantly younger at death than the moderate and non-brachycephalic group of dogs (median 12.7 years, IQR 11.1-15.0) (P \u3c 0.001). A higher proportion of deaths in extreme brachycephalic breed types were associated with URT disorders (4/24 deaths, 16.7 %) compared with the moderate and non-brachycephalic group (0/59 deaths, 0.0 %) (P = 0.001). The prevalence of having at least one URT disorder in the extreme brachycephalic group was higher (22.0 %, 95 % confidence interval (CI): 18.0-26.0) than in the moderate and non-brachycephalic group (9.7 %, 95 % CI: 7.1-12.3, P \u3c 0.001). The prevalence of URT disorders varied significantly by breed type: Bulldogs 19.5 %, French Bulldogs 20.0 %, Pugs 26.5 %, Border Terriers 9.0 %, West Highland White Terriers 7.0 % and Yorkshire Terriers 13.0 % (P \u3c 0.001). After accounting for the effects of age, bodyweight, sex, neutering and insurance, extreme brachycephalic dogs had 3.5 times (95 % CI: 2.4-5.0, P \u3c 0.001) the odds of at least one URT disorder compared with the moderate and non-brachycephalic group. Conclusions: In summary, this study reports that URT disorders are commonly diagnosed in Bulldog, French Bulldog, Pug, Border Terrier, WHWT and Yorkshire Terrier dogs attending primary-care veterinary practices in England. The three extreme brachycephalic breed types (Bulldog, French Bulldog and Pug) were relatively short-lived and predisposed to URT disorders compared with three other small-to-medium size breed types that are commonly owned (moderate brachycephalic Yorkshire Terrier and non-brachycephalic: Border Terrier and WHWT). Conclusions: In summary, this study reports that URT disorders are commonly diagnosed in Bulldog, French Bulldog, Pug, Border Terrier, WHWT and Yorkshire Terrier dogs attending primary-care veterinary practices in England. The three extreme brachycephalic breed types (Bulldog, French Bulldog and Pug) were relatively short-lived and predisposed to URT disorders compared with three other small-to-medium size breed types that are commonly owned (moderate brachycephalic Yorkshire Terrier and non-brachycephalic: Border Terrier and WHWT)

Placental syncytiotrophoblast constitutes a major barrier to vertical transmission of Listeria monocytogenes.

Listeria monocytogenes is an important cause of maternal-fetal infections and serves as a model organism to study these important but poorly understood events. L. monocytogenes can infect non-phagocytic cells by two means: direct invasion and cell-to-cell spread. The relative contribution of each method to placental infection is controversial, as is the anatomical site of invasion. Here, we report for the first time the use of first trimester placental organ cultures to quantitatively analyze L. monocytogenes infection of the human placenta. Contrary to previous reports, we found that the syncytiotrophoblast, which constitutes most of the placental surface and is bathed in maternal blood, was highly resistant to L. monocytogenes infection by either internalin-mediated invasion or cell-to-cell spread. Instead, extravillous cytotrophoblasts-which anchor the placenta in the decidua (uterine lining) and abundantly express E-cadherin-served as the primary portal of entry for L. monocytogenes from both extracellular and intracellular compartments. Subsequent bacterial dissemination to the villous stroma, where fetal capillaries are found, was hampered by further cellular and histological barriers. Our study suggests the placenta has evolved multiple mechanisms to resist pathogen infection, especially from maternal blood. These findings provide a novel explanation why almost all placental pathogens have intracellular life cycles: they may need maternal cells to reach the decidua and infect the placenta

Observable Signatures of Planet Accretion in Red Giant Stars I: Rapid Rotation and Light Element Replenishment

The orbital angular momentum of a close-orbiting giant planet can be sufficiently large that, if transferred to the envelope of the host star during the red giant branch (RGB) evolution, it can spin-up the star's rotation to unusually large speeds. This spin-up mechanism is one possible explanation for the rapid rotators detected among the population of generally slow-rotating red giant stars. These rapid rotators thus comprise a unique stellar sample suitable for searching for signatures of planet accretion in the form of unusual stellar abundances due to the dissemination of the accreted planet in the stellar envelope. In this study, we look for signatures of replenishment in the Li abundances and (to a lesser extent) 12C/13C, which are both normally lowered during RGB evolution. Accurate abundances were measured from high signal-to-noise echelle spectra for samples of both slow and rapid rotator red giant stars. We find that the rapid rotators are on average enriched in lithium compared to the slow rotators, but both groups of stars have identical distributions of 12C/13C within our measurement precision. Both of these abundance results are consistent with the accretion of planets of only a few Jupiter masses. We also explore alternative scenarios for understanding the most Li-rich stars in our sample---particularly Li regeneration during various stages of stellar evolution. Finally, we find that our stellar samples show non-standard abundances even at early RGB stages, suggesting that initial protostellar Li abundances and 12C/13C may be more variable than originally thought.Comment: Accepted for publication in the Astrophysical Journal. 29 pages in emulateapj format, including 16 figures and 12 tables. Tables 4 and 8 are provided in their entirety as plain text ancillary files (and will also be available in the electronic edition of ApJ

Another thread in the tapestry of stellar feedback: X-ray binaries

We consider X-ray binaries (XBs) as potential sources of stellar feedback. XBs observationally appear able to deposit a high fraction of their power output into their local interstellar medium, which may make them a non-negligible source of energy input. The formation rate of the most luminous XBs rises with decreasing metallicity, which should increase their significance during galaxy formation in the early universe. We also argue that stochastic effects are important to XB feedback (XBF) and may dominate the systematic changes due to metallicity in many cases. Large stochastic variation in the magnitude of XBF at low absolute star formation rates provides a natural reason for diversity in the evolution of dwarf galaxies which were initially almost identical, with several percent of such halos experiencing energy input from XBs roughly two orders of magnitude above the most likely value. These probability distributions suggest that the effect of XBF is most commonly significant for total stellar masses between ~10^7 and 10^8 Msun, which might resolve a current problem with modelling populations of such galaxies. We explain how XBs might inject energy before luminous supernovae (SNe) contribute significantly to feedback and how XBs can assist in keeping gas hot long after the last core-collapse SN has exploded. [...] XBF could be especially important to some dwarf galaxies, potentially heating gas without expelling it; the properties of XBF also match those previously derived as allowing episodic star formation. We also argue that the efficiency of SN feedback (SNF) might be reduced when XBF has had the opportunity to act first. In addition, we note that the effect of SNF is unlikely to be scale-free; galaxies smaller than ~100 pc might well experience less effective SNF. (Slightly abbreviated to fit arXiv size limit.)Comment: Very belatedly updated to include a note added in proof and additional reference. The definitive version is at: mnras.oxfordjournals.org/content/423/2/164