GESTÃO POR COMPETÊNCIAS NA ADMINISTRAÇÃO PÚBLICA: AVALIAÇÃO DE NECESSIDADES DE CAPACITAÇÃO COM BASE NA LACUNA DE COMPETÊNCIAS INDIVIDUAIS NA SECRETARIA DE GESTÃO DE PESSOAS DA UNIVASF

Esta pesquisa buscou verificar, com base na lacuna de competências individuais, as necessidades de capacitação dos Técnico-Administrativos em Educação lotados na Secretaria de Gestão de Pessoas (SGP) da Universidade Federal do Vale do São Francisco (Univasf). O estudo de natureza qualitativa foi realizado em cinco etapas: análise dos elementos estratégicos; identificação das competências setoriais; identificação das competências individuais; identificação da lacuna de competências; e identificação das necessidades de capacitação. Os instrumentos utilizados para a coleta de dados desta pesquisa foram análise documental, entrevistas semiestruturadas, grupo focal e questionário. Como resultado do estudo, foram identificadas 56 competências individuais da SGP, sendo que a maioria dessas competências apresentou lacuna com variação igual ou inferior a cinco, indicando pouca ou nenhuma necessidade de capacitação. Apenas cinco competências individuais apresentaram variação da lacuna entre 12 a 20, indicando uma grande necessidade de capacitação dessas competências prioritárias, todas da área de saúde

The Pauli principle, QRPA and the two-neutrino double beta decay

We examine the violation of the Pauli exclusion principle in the Quasiparticle Random Phase Approximation (QRPA) calculation of the two-neutrino double beta decay matrix elements, which has its origin in the quasi-boson approximation. For that purpose we propose a new renormalized QRPA with proton-neutron pairing method (full-RQRPA) for nuclear structure studies, which includes ground state correlation beyond the QRPA. This is achieved by using of renormalized quasi-boson approximation, in which the Pauli exclusion principle is taken into account more carefully. The full-RQRPA has been applied to two-neutrino double beta decay of $^{76}Ge$, $^{82}Se$, $^{128}Te$ and $^{130}Te$. The nuclear matrix elements have been found significantly less sensitive to the increasing strength of particle-particle interaction in the physically interesting region in comparison with QRPA results. The strong differences between the results of both methods indicate that the Pauli exclusion principle plays an important role in the evaluation of the double beta decay. The inclusion of the Pauli principle removes the difficulties with the strong dependence on the particle-particle strength $g_{pp}$ in the QRPA on the two-neutrino double beta decay.Comment: Accepted for publication in Nucl. Phys. A, 22 pages, including 5 figures, LaTeX (using REVTeX and epsfig-style

Epstein-Barr virus transcription factor Zta acts through distal regulatory elements to directly control cellular gene expression

Lytic replication of the human gamma herpes virus Epstein-Barr virus (EBV) is an essential prerequisite for the spread of the virus. Differential regulation of a limited number of cellular genes has been reported in B-cells during the viral lytic replication cycle. We asked whether a viral bZIP transcription factor, Zta (BZLF1, ZEBRA, EB1), drives some of these changes. Using genome-wide chromatin immunoprecipitation coupled to next-generation DNA sequencing (ChIP-seq) we established a map of Zta interactions across the human genome. Using sensitive transcriptome analyses we identified 2263 cellular genes whose expression is significantly changed during the EBV lytic replication cycle. Zta binds 278 of the regulated genes and the distribution of binding sites shows that Zta binds mostly to sites that are distal to transcription start sites. This differs from the prevailing view that Zta activates viral genes by binding exclusively at promoter elements. We show that a synthetic Zta binding element confers Zta regulation at a distance and that distal Zta binding sites from cellular genes can confer Zta-mediated regulation on a heterologous promoter. This leads us to propose that Zta directly reprograms the expression of cellular genes through distal elements

No Conclusive Evidence for Transits of Proxima b in MOST photometry

The analysis of Proxima Centauri's radial velocities recently led Anglada-Escud\'e et al. (2016) to claim the presence of a low mass planet orbiting the Sun's nearest star once every 11.2 days. Although the a-priori probability that Proxima b transits its parent star is just 1.5%, the potential impact of such a discovery would be considerable. Independent of recent radial velocity efforts, we observed Proxima Centauri for 12.5 days in 2014 and 31 days in 2015 with the MOST space telescope. We report here that we cannot make a compelling case that Proxima b transits in our precise photometric time series. Imposing an informative prior on the period and phase, we do detect a candidate signal with the expected depth. However, perturbing the phase prior across 100 evenly spaced intervals reveals one strong false-positive and one weaker instance. We estimate a false-positive rate of at least a few percent and a much higher false-negative rate of 20-40%, likely caused by the very high flare rate of Proxima Centauri. Comparing our candidate signal to HATSouth ground-based photometry reveals that the signal is somewhat, but not conclusively, disfavored (1-2 sigmas) leading us to argue that the signal is most likely spurious. We expect that infrared photometric follow-up could more conclusively test the existence of this candidate signal, owing to the suppression of flare activity and the impressive infrared brightness of the parent star.Comment: Accepted to ApJ. Posterior samples, MOST photometry and HATSouth photometry are all available at https://github.com/CoolWorlds/Proxim

Predictability of evolutionary trajectories in fitness landscapes

Experimental studies on enzyme evolution show that only a small fraction of all possible mutation trajectories are accessible to evolution. However, these experiments deal with individual enzymes and explore a tiny part of the fitness landscape. We report an exhaustive analysis of fitness landscapes constructed with an off-lattice model of protein folding where fitness is equated with robustness to misfolding. This model mimics the essential features of the interactions between amino acids, is consistent with the key paradigms of protein folding and reproduces the universal distribution of evolutionary rates among orthologous proteins. We introduce mean path divergence as a quantitative measure of the degree to which the starting and ending points determine the path of evolution in fitness landscapes. Global measures of landscape roughness are good predictors of path divergence in all studied landscapes: the mean path divergence is greater in smooth landscapes than in rough ones. The model-derived and experimental landscapes are significantly smoother than random landscapes and resemble additive landscapes perturbed with moderate amounts of noise; thus, these landscapes are substantially robust to mutation. The model landscapes show a deficit of suboptimal peaks even compared with noisy additive landscapes with similar overall roughness. We suggest that smoothness and the substantial deficit of peaks in the fitness landscapes of protein evolution are fundamental consequences of the physics of protein folding.Comment: 14 pages, 7 figure

Analytic descriptions for transitional nuclei near the critical point

Exact solutions of the Bohr Hamiltonian with a five-dimensional square well potential, in isolation or coupled to a fermion by the five-dimensional spin-orbit interaction, are considered as examples of a new class of dynamical symmetry or Bose-Fermi dynamical symmetry. The solutions provide baselines for experimental studies of even-even [E(5)] and odd-mass [E(5|4)] nuclei near the critical point of the spherical to deformed gamma-unstable phase transition.Comment: LaTeX (elsart), 53 pages; typographical correction to (3.15

Many-body correlations in a multistep variational approach

We discuss a multistep variational approach for the study of many-body correlations. The approach is developed in a boson formalism (bosons representing particle-hole excitations) and based on an iterative sequence of diagonalizations in subspaces of the full boson space. Purpose of these diagonalizations is that of searching for the best approximation of the ground state of the system. The procedure also leads us to define a set of excited states and, at the same time, of operators which generate these states as a result of their action on the ground state. We examine the cases in which these operators carry one-particle one-hole and up to two-particle two-hole excitations. We also explore the possibility of associating bosons to Tamm-Dancoff excitations and of describing the spectrum in terms of only a selected group of these. Tests within an exactly solvable three-level model are provided.Comment: 24 pages, 6 figures, to appear in Phys. Rev.

Simultaneous Dual Frequency Observations of Giant Pulses from the Crab Pulsar

Simultaneous measurements of giant pulses from the Crab pulsar were taken at two widely spaced frequencies using the real-time detection of a giant pulse at 1.4 GHz at the Very Large Array to trigger the observation of that same pulse at 0.6 GHz at a 25-m telescope in Green Bank, WV. Interstellar dispersion of the signals provided the necessary time to communicate the trigger across the country via the Internet. About 70% of the pulses are seen at both 1.4 GHz and 0.6 GHz, implying an emission mechanism bandwidth of at least 0.8 GHz at 1 GHz for pulse structure on time scales of one to ten microseconds. The arrival times at both frequencies display a jitter of 100 microseconds within the window defined by the average main pulse profile and are tightly correlated. This tight correlation places limits on both the emission mechanism and on frequency dependent propagation within the magnetosphere. At 1.4 GHz the giant pulses are resolved into several, closely spaced components. Simultaneous observations at 1.4 GHz and 4.9 GHz show that the component splitting is frequency independent. We conclude that the multiplicity of components is intrinsic to the emission from the pulsar, and reject the hypothesis that this is the result of multiple imaging as the signal propagates through the perturbed thermal plasma in the surrounding nebula. At both 1.4 GHz and 0.6 GHz the pulses are characterized by a fast rise time and an exponential decay time which are correlated. The pulse broadening with its exponential decay form is most likely the result of multipath propagation in intervening ionized gas.Comment: LaTeX, 18 pages, 7 figures, accepted for publication in The Astrophysical Journa

A single residue substitution in the receptor-binding domain of H5N1 hemagglutinin is critical for packaging into pseudotyped lentiviral particles

© 2012 Tang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Background: Serological studies for influenza infection and vaccine response often involve microneutralization and hemagglutination inhibition assays to evaluate neutralizing antibodies against human and avian influenza viruses, including H5N1. We have previously characterized lentiviral particles pseudotyped with H5-HA (H5pp) and validated an H5pp-based assay as a safe alternative for high-throughput serological studies in BSL-2 facilities. Here we show that H5-HAs from different clades do not always give rise to efficient production of H5pp and the underlying mechanisms are addressed. Methodology/Findings: We have carried out mutational analysis to delineate the molecular determinants responsible for efficient packaging of HA from A/Cambodia/40808/2005 (H5Cam) and A/Anhui/1/2005 (H5Anh) into H5pp. Our results demonstrate that a single A134V mutation in the 130-loop of the receptor binding domain is sufficient to render H5Anh the ability to generate H5Anh-pp efficiently, whereas the reverse V134A mutation greatly hampers production of H5Cam-pp. Although protein expression in total cell lysates is similar for H5Anh and H5Cam, cell surface expression of H5Cam is detected at a significantly higher level than that of H5Anh. We further demonstrate by several independent lines of evidence that the behaviour of H5Anh can be explained by a stronger binding to sialic acid receptors implicating residue 134. Conclusions: We have identified a single A134V mutation as the molecular determinant in H5-HA for efficient incorporation into H5pp envelope and delineated the underlying mechanism. The reduced binding to sialic acid receptors as a result of the A134V mutation not only exerts a critical influence in pseudotyping efficiency of H5-HA, but has also an impact at the whole virus level. Because A134V substitution has been reported as a naturally occurring mutation in human host, our results may have implications for the understanding of human host adaptation of avian influenza H5N1 virusesThis work was supported by grants from the Research Fund for the Control of Infectious Diseases of Hong Kong (RFCID#08070972), the Area of Excellence Scheme of the University Grants Committee (grant AoE/M-12/-06 of the Hong Kong Special Administrative Region, China), the French Ministry of Health, and the RESPARI project of the Institut Pasteur International Network

Bayesian fitting of Taurus brown dwarf spectral energy distributions

We present derived stellar and disc parameters for a sample of Taurus brown dwarfs both with and without evidence of an associated disc. These parameters have been derived using an online fitting tool (http://bd-server.astro.ex.ac.uk/), which includes a statistically robust derivation of uncertainties, an indication of pa- rameter degeneracies, and a complete treatment of the input photometric and spectroscopic observations. The observations of the Taurus members with indications of disc presence have been fitted using a grid of theoretical models including detailed treatments of physical processes accepted for higher mass stars, such as dust sublimation, and a simple treatment of the accretion flux. This grid of models has been designed to test the validity of the adopted physical mechanisms, but we have also constructed models using parameterisation, for example semi-empirical dust sublimation radii, for users solely interested in parameter derivation and the quality of the fit. The parameters derived for the naked and disc brown dwarf systems are largely consistent with literature observations. However, our inner disc edge locations are consistently closer to the star than previous results and we also derive elevated accretion rates over non-SED based accretion rate derivations. For inner edge locations we attribute these differences to the detailed modelling we have performed of the disc structure, particularly at the crucial inner edge where departures in geometry from the often adopted vertical wall due to dust sublimation (and therefore accretion flux) can compensate for temperature (and therefore distance) changes to the inner edge of the dust disc. In the case of the elevated derived accretion rates, in some cases, this may be caused by the intrinsic stellar luminosities of the targets exceeding that predicted by the isochrones we have adopted.Comment: The paper contains 35 pages with 15 figures and 17 tables. Accepted for publication in MNRA