Historical biogeography and ecological niche modelling of the Asimina-Disepalum clade (Annonaceae): role of ecological differentiation in Neotropical-Asian disjunctions and diversification in Asia

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Optic flow information influencing heading perception during rotation

Poster Session - Perception and Action: abstract no. 22.34We investigated what roles global spatial frequency, surface structure, and foreground motion play in heading perception during simulated rotation from optic flow. The display …postprin

Phylogenetic Reconstruction, Morphological Diversification and Generic Delimitation of Disepalum (Annonaceae)

Taxonomic delimitation of Disepalum (Annonaceae) is contentious, with some researchers favoring a narrow circumscription following segregation of the genus Enicosanthellum. We reconstruct the phylogeny of Disepalum and related taxa based on four chloroplast and two nuclear DNA regions as a framework for clarifying taxonomic delimitation and assessing evolutionary transitions in key morphological characters. Maximum parsimony, maximum likelihood and Bayesian methods resulted in a consistent, well-resolved and strongly supported topology. Disepalum s.l. is monophyletic and strongly supported, with Disepalum s. str. and Enicosanthellum retrieved as sister groups. Although this topology is consistent with both taxonomic delimitations, the distribution of morphological synapomorphies provides greater support for the inclusion of Enicosanthellum within Disepalum s.l. We propose a novel infrageneric classification with two subgenera. Subgen. Disepalum (= Disepalum s. str.) is supported by numerous synapomorphies, including the reduction of the calyx to two sepals and connation of petals. Subgen. Enicosanthellum lacks obvious morphological synapomorphies, but possesses several diagnostic characters (symplesiomorphies), including a trimerous calyx and free petals in two whorls. We evaluate changes in petal morphology in relation to hypotheses of the genetic control of floral development and suggest that the compression of two petal whorls into one and the associated fusion of contiguous petals may be associated with the loss of the pollination chamber, which in turn may be associated with a shift in primary pollinator. We also suggest that the formation of pollen octads may be selectively advantageous when pollinator visits are infrequent, although this would only be applicable if multiple ovules could be fertilized by each octad; since the flowers are apocarpous, this would require an extragynoecial compitum to enable intercarpellary growth of pollen tubes. We furthermore infer that the monocarp fruit stalks are likely to have evolved independently from those in other Annonaceae genera and may facilitate effective dispersal by providing a color contrast within the fruit.published_or_final_versio

Deterministic Assembly of Arrays of Lithographically Defined WS2 and MoS2 Monolayer Features Directly from Multilayer Sources into Van der Waals Heterostructures

One of the major challenges in the van der Waals (vdW) integration of two-dimensional (2D) materials is achieving high-yield and high-throughput assembly of predefined sequences of monolayers into heterostructure arrays. Mechanical exfoliation has recently been studied as a promising technique to transfer monolayers from a multilayer source synthesized by other techniques, allowing the deposition of a wide variety of 2D materials without exposing the target substrate to harsh synthesis conditions. Although a variety of processes have been developed to exfoliate the 2D materials mechanically from the source and place them deterministically onto a target substrate, they can typically transfer only either a wafer-scale blanket or one small flake at a time with uncontrolled size and shape. Here, we present a method to assemble arrays of lithographically defined monolayer WS2 and MoS2 features from multilayer sources and directly transfer them in a deterministic manner onto target substrates. This exfoliate-align-release process - without the need of an intermediate carrier substrate - is enabled by combining a patterned, gold-mediated exfoliation technique with a new optically transparent, heat-releasable adhesive. WS2/MoS2 vdW heterostructure arrays produced by this method show the expected interlayer exciton between the monolayers. Light-emitting devices using WS2 monolayers were also demonstrated, proving the functionality of the fabricated materials. Our work demonstrates a significant step toward developing mechanical exfoliation as a scalable dry transfer technique for the manufacturing of functional, atomically thin materials

Historical biogeography of Asimina-Disepalum (Annonaceae): origins of tropical intercontinental disjunctions and diversifications in Southeast Asia

Oral Session: Secondary forestsStudies of tropical intercontinental disjunctions, common biogeographical patterns in flowering plants, enable insights into the origin of extant distributions and diversity patterns. We investigate the temporal origin of the Neotropical-Asian disjunction in the Asimina-Disepalum clade of the early-divergent angiosperm family Annonaceae, and temporal congruence with underlying palaeoclimatic and geological processes. Moreover, we aim to identify the geographical origin of Disepalum, and to infer dispersal and vicariance events as well as potentially correlated climate niche differentiation in the evolution of the genus in …postprin

Disentangling the effects of object position and motion on heading judgments in the presence of a moving object

Tuesday Morning Posters - Motion Perception: Optic flow and heading: no. 53.4026Previous research has found that moving objects bias heading perception only when they occlude the focus of expansion (FOE) in the background optic flow, with the direction of bias depending on whether the moving object was approached or at a fixed distance from the moving observer. However, the effect of object motion on heading perception was confounded with object position in previous studies. Here, we disentangled the contributions of object motion and position to heading bias. In each 1s trial, the display simulated forward observer motion at 1 m/s through a ...postprin

Inkjet printed multimetal microelectrodes on PDMS for functionalized microfluidic systems

A novel direct method of metal microelectrode patterning on polydimethylsiloxane (PDMS) using inkjet printed gold and silver nanoparticles to form electrochemical sensors is presented. Inkjet printing is an additive microfabrication technique enabling microelectrode patterning directly over large areas at low-temperatures. (3-mercaptopropyl) trimethoxysilane (MPTMS) to promote PDMS surface wettability and improve metal adhesion and a pixel-printing subsampling method to overcome surface tension driven ink-droplet coalescence, are then employed to form a robust fabrication process. The resulting printed gold and silver microelectrodes exhibit good compactness, continuity and conductivity, and are used to manufacture functionalized microfluidic systems with in-situ three-electrode electrochemical sensors.published_or_final_versio

Two-photon excited photoluminescence in InGaN multi-quantum-wells structures

In this work, we report on the two-photon absorption induced luminescence of InGaN multiple quantum wells grown on sapphire. When the sample was excited by femtosecond near-infrared laser pulses at room temperature, an intense luminescence signal peaked at ∼415 nm from the sample was observed, which indicates strong nonlinear optical effect in InGaN quantum well structures. The interferometric autocorrelated luminescence traces were recorded to verify the second order nonlinearity of the luminescence. In addition, the strong second harmonic generation signal of the excitation laser was also observed. The mechanism of the two-photon excited photoluminescence in InGaN quantum wells was discussed.published_or_final_versionpublished_or_final_versio

In the fetal thymus, Gli3 in thymic epithelial cells promotes thymocyte positive selection and differentiation by repression of Shh

Gli3 is a Hedgehog (Hh) responsive transcription factor that can function as a transcriptional repressor or activator. We show that Gli3 activity in thymic epithelial cells (TEC) promotes positive selection and differentiation from CD4+CD8+ to CD4+CD8- single positive (SP4) cell in the fetal thymus and that Gli3 represses Shh Constitutive deletion of Gli3, and conditional deletion of Gli3 from TEC, reduced differentiation to SP4, whereas conditional deletion of Gli3 from thymocytes did not. Conditional deletion of Shh from TEC increased differentiation to SP4, and expression of Shh was upregulated in the Gli3-deficient thymus. Use of a transgenic Hh-reporter showed that the Hh pathway was active in thymocytes, and increased in the Gli3-deficient fetal thymus. Neutralisation of endogenous Hh proteins in the Gli3-/- thymus restored SP4 differentiation, indicating that Gli3 in TEC promotes SP4 differentiation by repression of Shh Transcriptome analysis showed that Hh-mediated transcription was increased but TCR-mediated transcription decreased in Gli3-/- thymocytes compared to WT