Linking activity, composition and seasonal dynamics of atmospheric methane oxidizers in a meadow soil

Microbial oxidation is the only biological sink for atmospheric methane. We assessed seasonal changes in atmospheric methane oxidation and the underlying methanotrophic communities in grassland near Giessen (Germany), along a soil moisture gradient. Soil samples were taken from the surface layer (0–10 cm) of three sites in August 2007, November 2007, February 2008 and May 2008. The sites showed seasonal differences in hydrological parameters. Net uptake rates varied seasonally between 0 and 70 μg CH(4) m(−2) h(−1). Greatest uptake rates coincided with lowest soil moisture in spring and summer. Over all sites and seasons, the methanotrophic communities were dominated by uncultivated methanotrophs. These formed a monophyletic cluster defined by the RA14, MHP and JR1 clades, referred to as upland soil cluster alphaproteobacteria (USCα)-like group. The copy numbers of pmoA genes ranged between 3.8 × 10(5)–1.9 × 10(6) copies g(−1) of soil. Temperature was positively correlated with CH(4) uptake rates (P<0.001), but had no effect on methanotrophic population dynamics. The soil moisture was negatively correlated with CH(4) uptake rates (P<0.001), but showed a positive correlation with changes in USCα-like diversity (P<0.001) and pmoA gene abundance (P<0.05). These were greatest at low net CH(4) uptake rates during winter times and coincided with an overall increase in bacterial 16S rRNA gene abundances (P<0.05). Taken together, soil moisture had a significant but opposed effect on CH(4) uptake rates and methanotrophic population dynamics, the latter being increasingly stimulated by soil moisture contents >50 vol% and primarily related to members of the MHP clade

Evidence of topographic disequilibrium in the Subarnarekha River Basin, India: A digital elevation model based analysis

Cratonic areas experience complex process-response changes due to their operative endogenic and exogenic forces varying in intensity and spatiality over long timescales. Unlike zones of active deformation, the surface expression of the transient signals in relatively tectonically stable areas are usually scant. The Subarnarekha River Basin, in eastern India, is a prime example of a Precambrian cratonic landscape, overlain in places by Tertiary and Quaternary deposits. A coupled quantitative-qualitative approach is employed towards deciphering tectonic and geological influences across linear and areal aspects, at the basin and sub-basin scale. Within this landscape, the transient erosional signatures are explored, as recorded in the disequilibrium conditions of the longitudinal profiles of the major streams, which are marked by a number of waterfalls at structural and lithological boundaries. Mathematical expressions derived from the normalized longitudinal profiles of these streams are used to ascertain their stage of development. Cluster analysis and chi plots provide significant interpretations of the role of vertical displacements or litho-structural variations within the basin. These analyses suggest that a heterogeneous, piece-meal response to the ongoing deformation exists in the area, albeit, determining the actual rate of this deformation or its temporal variation is difficult without correlated chronological datasets.by Shantamoy Guha and Priyank Pravin Pate