Lectura de los escritos de Elena G. de White y su relación con el compromiso misionero y la fidelidad en diezmos y ofrendas en feligreses de la Asociación Peruana Central Este, Lima, Perú 2020

En el presente trabajo de investigación realizado sobre la lectura de los escritos de Elena G. de White y su relación con el compromiso misionero y la fidelidad en diezmos y ofrendas en feligreses de la Asociación Peruana Central Este, Lima, Perú 2020, se tuvo la participación de 566 personas pertenecientes a la Iglesia Adventista. El contenido de este artículo está dividido en tres partes principales. La primera una breve introducción que brindará un marco a la problemática a tratar. La segunda contiene la metodología que se utilizó para la investigación y por último el análisis de los resultados obtenidos junto a la conclusión.LIMAEscuela de PosgradoInvestigación bíblico-aplicad

Novel hepatocyte-like liver organoids recapitulate crucial mature hepatic functions

Accurate liver disease modeling and drug toxicity testing still remain challenging as liver cells in vitro poorly resemble adult hepatocytes, as we previously demonstrated using whole transcriptome and cell identity analysis. To address this, we used our insights into hepatic modeling to develop hepatocyte-like liver organoids (HeLLOs), a novel human organoid model with mature hepatocyte functions superior to existing models. HeLLOs are easily established from (small) healthy or diseased liver tissues and rapidly expanded for an extended period in optimized culture conditions. Transcriptomic and functional analyses revealed that differentiated HeLLOs closely resemble fresh primary human hepatocytes (PHHs) and perform key hepatic functions such as gluconeogenesis, drug metabolism, and bile acid synthesis. We developed a HeLLO-based toxicity assay with higher sensitivity in predicting liver toxicity of known liver-toxic drugs compared to the gold-standard PHHs. By modeling disease-related mechanisms, such as bile acid transport, HeLLOs uncover transport-inhibition toxicity mechanisms of known liver toxic drugs. Single cell sequencing analysis of HeLLOs identified a heterogeneous cluster of cells with cholangiocyte-like and hepatocyte-like cells, overall resembling liver regenerative cells. As such, HeLLOs hold great promise for advancing liver disease modeling and drug testing. To our knowledge, HeLLOs are the best expandable liver model for predicting adverse drug reactions as well as modeling various liver disease mechanisms

The slaves, the state and the church : slavery and amelioration in Jamaica 1797-1833

This study explores slave agency and slave abolitionism during amelioration in Jamaica. The amelioration period was chosen because it offered the slave opportunities to acquire their freedom and improve their condition. Therefore, slave agency and abolitionism occurred more frequently after the start of amelioration, which officially began in Jamaica in 1797 when the planters embarked on a programme designed to improve slavery and prolong its existence. Amelioration continued until the British Parliament voted to abolish slavery in 1833.EThOS - Electronic Theses Online ServiceGBUnited Kingdo