Selective Oxidation of Pregabalin Drug Molecule: Exploration of Scope, Reaction Mechanism and Kinetic Modeling

The kinetics and mechanism of (RuCl3 + OsO4) in combination and, RuCl3 and OsO4 alone catalyzed oxidation of pregabalin (PGB) drug with chloramine-T have been investigated at 313 K in aqueous alkaline medium. The kinetic characteristics were found to be varied for each catalyzed reactions. In all the three catalyzed reactions, the reaction rate shows a first-order dependence of rate on [CAT]0 and a negative-fractional-order on [NaOH]. The order of [PGB]0 is found to be unity incase of [OsO4], but it is fractional in both RuCl3 and [RuCl3 + OsO4] catalyzed reactions. The orders with respect to [RuCl3] and [OsO4] are less than one whereas it is unity in case of [RuCl3 + OsO4]. Activation parameters have been evaluated. 2-isobutylsuccinic acid was identified as the oxidation product of PGB. Under identical set of experimental conditions, the reaction rates revealed that all the three catalyzed reactions are about 25 to 71-fold faster than the uncatalyzed reactions. The catalytic efficiency of these catalysts follows the order (RuCl3 + OsO4) > OsO4 > RuCl3. The observed reactivity sequence may be attributed to the different d-electronic configuration of the catalysts. Most noteworthy is the significant catalytic activity of 71-fold in case of (RuCl3 + OsO4) catalyst. It justifies the synergistic effect of (RuCl3 + OsO4) catalyst on the oxidation of PGB drug. An isokinetic relationship is observed with ß = 366 K, indicating that enthalpy factors are controlling the rate. The reaction mechanisms put forward and rigorous kinetic models deduced, give the best fit to the experimental results for each catalyzed reactions

Mechanistic chemistry of oxidation of balsalazide with acidic chloramine-​T and bromamine-​T: A comparative spectrophotometric kinetic study

Balsalazide (BSZ) belongs to a class of non-​steroidal anti-​inflammatory drugs. Kinetics and mechanism of oxidn. of BSZ with sodium N-​halo-​p-​toluenesulfonamides viz., chloramine-​T (CAT) and bromamine-​T (BAT) in HClO4 medium have been spectrophotometrically investigated (λmax = 357 nm) at 303 K. Under comparable exptl. conditions, reactions with both the oxidants follow a first-​order dependence of rate on [BSZ] and fractional-​order dependence on each [oxidant] and [HClO4]​. Activation parameters and reaction consts. have been computed. 2-​hydroxy-​5-​nitrosobenzoic acid and 3-​(4-​nitroso-​benzoylamino)​propionic acid are identified as the oxidn. products of BSZ with both CAT and BAT. The rate of oxidn. of BSZ is about five-​fold faster with BAT than with CAT. Plausible mechanism and related rate law have been deduced for the obsd. kinetics

Evaluation of bacteriological diagnosis of smear positive pulmonary tubreculosis under programme conditions in three districts in the context of DOTS implementation in India

Objective: To study the smear and culture positivity rates in pulmonary tuberculosis patients declared as smear positive in the districts of North Arcot (Tamil Nadu), Raichur (Karnataka) and Wardha (Maharashtra) in India in order to evaluate the diagnosis of pulmonary tuberculosis at the field level under programme conditions. Methods: Two specimens of sputum from each of 320 patients in North Arcot, 314 patients in Raichur and 302 patients from Wardha district, all of whom had been reported as smear-positive at the field level, were examined by smear and culture. Findings: The proportion of specimens found to be smear-negative was 4.7% in North Arcot and 5.7% in Raichur as against 38.7% in Wardha. The proportions of culture negative specimens were 5.7% and 6.3% respectively in North Arcot and Raichur, while it was 35.6% at Wardha. The difference in the smear and culture negativity between Wardha and the other two districts was highly significant. Conclusions: The study revealed an unacceptably high level of false positives in sputum smear microscopy in the Wardha district. This could be attributed to the absence of systematic and intensive training in smear examination consequent to the non-implementation of the DOTS strategy in this district and a high standard of training offered in the RNTCP implemented districts

Comparison of different methods of assessing in vitro resistance of Mycobacterium tuberculosis to rifampicin

Background & objectives: Definitions of in vitro resistance to rifampicin in. strains of Mycobacterium tuberculosis by different methods have not been consistent, leading to variations in the interpretation and validity of results. This study compared three methods of defining in vitro resistance to rifampicin. Methods: (i) A total of 598 clinical isolates of M. tuberculosis were concurrently compared by the minimal inhibitory concentration (MIC) and the proportion method on Lowenstein-Jensen medium; (ii) 54 strains tested by the MIC method were retested by the proportion method and the BACTEC radiometric method; and (iii) 72 strains which yielded an MIC of 64 mg/l by the MIC method were retested by the same method. Results: Out of 598 cultures tested by the MIC and the proportion methods, identical classification as susceptible or resistant was observed in 99.7 per cent. A 100 per cent agreement was observed when 54 strains were tested by the MIC, proportion and BACTEC radiometric methods. When 72 strains with an MIC of 64 mg/l were retested by the same method, 61 (85%) yielded a lower MIC, 9 (12%) gave the same MIC while 2 (3%) yielded a higher MIC of 128 mg/l, reflecting perhaps the inherent limitations of the variations in the inoculum size. Interpretation & conclusion: All 3 definitions of resistance, viz., an MIC of 128 mg/l, a proportion of I per cent or more on 40 mg/l by the proportion method, both on L-J medium and a growth of 1 per cent or more on 2 mg/l by the radiometric method were found to be equally satisfactory

A Finite Element Analysis to Compare Three Dimensional Stress Distribution in Different Bone Densities by Extra Short Implants with Varying Abutment Lengths and Diameters.

Introduction: Dental implants have revolutionized the rehabilitation of partially and completely edentulous patients, offering a functional and aesthetic alternative to traditional prostheses. However, in patients with severe alveolar bone resorption, placing standard-length implants can be challenging due to limited vertical bone height. This often necessitates invasive procedures such as bone grafting or sinus augmentation, which may not be feasible in all clinical scenarios due to increased cost, surgical morbidity, or systemic contraindications. Objectives: To evaluate and compare three-dimensional stress distribution in different bone densities (D1, D2, D3, D4) using extra-short dental implants with varying abutment lengths and diameters through Finite Element Analysis. Methods: A total of 24 three-dimensional models were created using CAD software to simulate extra-short implants (6 mm length) with varying abutment diameter and length (5mmx5mm, 5mmx6.5mm, 5mmx10mm, 5mmx12mm, 6.5mmx6.5mm, 7.5mmx8mm). These implant-abutment assemblies were placed in bone models representing D1, D2, D3, and D4 bone densities. Finite Element Analysis was conducted under axial and oblique loading conditions. The von Mises stress values were evaluated at the implant, abutment, and crestal bone regions. Results: Implants with larger diameters showed reduced stress in all bone types, particularly at the crestal bone level. Higher abutment heights increased stress concentration, especially in oblique loading scenarios. Among the bone types, D2 bone showed the most favorable stress distribution, while D4 bone exhibited the highest stress values, indicating a higher risk of biomechanical failure. Conclusions: Bone density plays a critical role in stress distribution around extra-short implants. Increased implant diameter can enhance stress dissipation, while taller abutments may compromise stability in low-density bone. Optimal implant-abutment combinations must be selected based on bone quality to improve long-term success in atrophic jaw rehabilitations using extra-short implants

Detection of injection attacks on in-vehicle network using data analytics

We investigate the possibility of detection of injection attacks using data analytics techniques in this thesis. The automotive industry is innovating the modern vehicles towards connectivity by interfacing them with various external entities. These entities are exposing the automobile to cyber attacks instead of ensuring its safety. Therefore it is important to consider the security aspect while developing these interfaces. Firstly, we try understand the automobile network architecture and the possible security threats associated with it. Next, we examine the various possible cyber-attacks on automobiles described in the literature. We experiment and analyze the attack scenarios by performing injection attacks on a vehicle. We collect the data during the injection attacks and apply multiple data analysis techniques. These techniques build a model based on data during normal operation. The observations from the data collected during injection attacks is fit into these techniques. The data points that do not fit the model are termed as attack points. Finally we examine and analyze the results and their accuracy in detecting injection attacks

Origin of accessory left hepatic artery from left gastric artery

Liver is supplied by the branches of celiac trunk. Common hepatic artery which is a branch of celiac trunk continues as proper hepatic artery after giving gastroduodenal artery. Proper hepatic artery enters the liver at Porta hepatis after diving into right and left hepatic artery. The knowledge of branching patterns of arteries and their variations is important in various surgical and radiological procedures. During routine dissection conducted in the Department of Anatomy, MMC&RI, Mysore, an accessory left hepatic artery was seen arising from left gastric artery in an elderly male cadaver aged around 60 years. An accessory left hepatic artery was arising from left gastric artery and was entering the left lobe of liver. In less than 1% of cases, the accessory left hepatic artery supplies the part of left lobe of liver or whole liver. Knowledge of anomalous origin of left hepatic artery is important for successful liver transplant surgeries, hepatobiliary operations, gastrectomies, hiatal surgery for gastro esophageal reflex, bariatric surgeries and in selective arterial chemotherapy for liver cancers. Ligation or laceration of this artery during surgeries causes fatal ischemic necrosis of left lobe of liver

Morphometric features of asterion in adult human skulls

Background: Asterion is the meeting point of temporal, occipital and parietal bones on the posterolateral surface of skull and surgically an important point of reference for approaching the posterior cranial fossa structures. However surgeons have been skeptical about its reliability due to population based differences in its morphology, distance with other external landmarks and also to sigmoid and transverse sinuses.Methods: In this study 50 (27 male & 23 female) adult skulls were investigated to determine the type of asterion, its distance from important bony landmarks and also the nearby venous sinuses were measured.Results: Our study revealed that type II (absence of sutural bones) was commoner than type I (presence of sutural bones) asterion. The asterion was 4.82 ± 0.58 cm from tip of the mastoid process on the right side and 4.70 ± 0.70 cm on the left. It was greater in males than in females, p value being statistically significant (P = 0.00 & P = 0.02 for right & left sides respectively). The distance of asterion from supramastoid crest was 4.22 ± 0.73 cm on the right and 4.23+/-0.58 cm on the left. The distance in males was more than in females. The P value 0.00 was statistically significant on the right side. Regarding the position of the asterion in relation to transverse sinus, it was on the transverse sinus in 62% cases, below it in 32% and above in 6%.Conclusions: The data obtained shows that the asterion is located either at the level or below the level of the transverse sinus in majority of the cases. This information is useful to neurosurgeons to reduce the risk during posterior cranial fossa surgeries. This work will also be useful to anthropologists, forensic science experts for determination of sex of the skull along with other parameters.

Bilateral high division of sciatic nerve

Sciatic nerve is the thickest nerve in the body formed by the sacral plexus from L4 to S3 in the lesser pelvis. It emerges through the greater sciatic foramen below the piriformis and enter the gluteal region. Then the nerve passes on the back of the thigh and at the level of superior angle of popliteal fossa it terminates by dividing into tibial and common peroneal nerve. The knowledge of anatomical variations in the division of nerve is important for various surgical and anaesthetic procedures. During routine dissection in the department of anatomy, Mysore Medical College & Research Institute, Mysore, a rare bilateral high division of sciatic nerve was observed in a female cadaver aged about 40 years. In the present case there was bilateral high division of sciatic nerve. The nerve was seen dividing into two branches before it emerges through the greater sciatic foramen. The tibial nerve was entering the gluteal region below the piriformis muscle and common peroneal nerve was entering by piercing the piriformis. The knowledge of this variation is important as the nerve may get compressed with surrounding anatomical structures resulting in non discogenic sciatica. The awareness of variations is important for surgeons during various procedures like fracture, posterior dislocation of hip joint and hip joint replacement. The anatomical variations are important during deep intramuscular injections in gluteal region and also for anaesthetists during sciatic nerve block

Situs inversus

Situs inversus is a rare condition with a genetic predisposition, where in organs are transposed from their normal location to the opposite side of the body with an incidence of 1 in 10000 live births. If Situs Inversus is associated with other congenital anomalies the survival rate is low. So prenatal diagnosis is essential to detect any associated anomalies. During routine fetal autopsies conducted in the department of anatomy, Mysore medical college, Mysore, a rare interesting case of still born baby with gestational age of 20 weeks was observed. Autopsy findings of external examination revealed kyphosis in thoracic region. Thoracotomy revealed heart on the right side (dextrocardia). Laparotomy revealed that the left lobe of liver was bigger than the right. The gall bladder was present on the visceral surface of the left lobe of liver, stomach was on right side, duodenum on left side, pancreas and spleen were on the right side. There was agenesis of right kidney, right ureter and both suprarenal glands. All the above findings correlate with the condition situs inversus. Situs inversus is a rare condition with a genetic predisposition, it’s etiology lies in the mutation of chromosome no 12, which is critical for recognition of right sidedness.1,2 Even though there is transposition of organs in situs inversus, the survival rate is good but if situs inversus is associated with other congenital anomalies the survival rate is low. So prenatal diagnosis is essential to detect any associated anomalies to bring down mortality rate