Different Class: The Creation of the Premier League and the Commercialization of English Football

This project examines how English football evolved from a culture of hooliganism and poor upkeep into a popular and enterprising industry across the globe. The Premier League and its stars marketed the English game and its culture worldwide. Since the 1990s England has established itself as the leading club footballing nation. I argue that through football, and the culture and economics behind it, we can see the ways in which England attempted to change its image in the modern world. In the 1980s and 1990s Britain was confronted with its own established culture of violence, bigotry, and nationalist pride, particularly the sport of football. English football clubs and the English Football Association (FA) adapted in an effort to change their image and create a more accessible and marketable product. This study examines those changes and the ways in which they impacted the league, clubs, and fan culture in footballing communities. With a limited and economics-focused historiography on the subject, this work will contribute to the discussion by exploring a cultural perspective and examining the changes and economic impact from club and fan levels. It will also place this evolution within a broader European cultural context

Entrepreneurs as influencers: the impact of parasocial interactions on communication outcomes

Purpose: Drawing on the example of car manufacturer Tesla and its early investor Elon Musk, the purpose of this paper is to explore the connection between the personal communication activities of influential entrepreneurs on social media, the emergence of parasocial interactions (PSIs) and the related communication outcomes for the company. Design/methodology/approach: This paper conducted an online survey, recruiting 207 participants via purposive sampling. Partial least square path modeling and an independent t-test were conducted to test hypotheses. Findings: The results of this paper show that following entrepreneurs' personal social media activities amplifies PSIs, which in turn positively impact the company's communication outcomes. Organization-public relationships and purchase intentions are improved by PSI. Originality/value: To the best of the authors’ knowledge, this is one of the first studies that connects the personal and the organizational level in exploring entrepreneurial marketing. The results show that Elon Musk acts as an influential entrepreneur to effectively promote communication outcomes for Tesla. This paper illuminates the potential of entrepreneurs' personal social media activities to support the success of their ventures

A telephone survey of cancer awareness among frontline staff: informing training needs

Background: Studies have shown limited awareness about cancer risk factors among hospital-based staff. Less is known about general cancer awareness among community frontline National Health Service and social care staff. Methods: A cross-sectional computer-assisted telephone survey of 4664 frontline community-based health and social care staff in North West England. Results: A total of 671 out of 4664 (14.4%) potentially eligible subjects agreed to take part. Over 92% of staff recognised most warning signs, except an unexplained pain (88.8%, n=596), cough or hoarseness (86.9%, n=583) and a sore that does not heal (77.3%, n=519). The bowel cancer-screening programme was recognised by 61.8% (n=415) of staff. Most staff agreed that smoking and passive smoking ‘increased the chance of getting cancer.’ Fewer agreed about getting sunburnt more than once as a child (78.0%, n=523), being overweight (73.5%, n=493), drinking more than one unit of alcohol per day (50.2%, n=337) or doing less than 30 min of moderate physical exercise five times a week (41.1%, n=276). Conclusion: Cancer awareness is generally good among frontline staff, but important gaps exist, which might be improved by targeted education and training and through developing clearer messages about cancer risk factors

Concepção e implementação de experiências laboratoriais sobre MPLS

Mestrado em Engenharia Electrónica e TelecomunicaçõesO Multiprotocol Label Switching (MPLS) é um mecanismo de transporte de dados, sob a forma de um protocolo agnóstico, com grande potencial de crescimento e adequação. Opera na “Camada 2.5” do modelo OSI e constitui um mecanismo de alto desempenho utilizado nas redes de núcleo para transportar dados de um nó da rede para outro. O sucesso do MPLS resulta do facto de permitir que a rede transporte todos os tipos de dados, desde tráfego IP a tráfego da camada de ligação de dados, devido ao encapsulamento dos pacotes dos diversos protocolos, permitindo a criação de “links virtuais” entre nós distantes. O MPLS pertence à família das “redes de comutação de pacotes”, sendo os pacotes de dados associados a “etiquetas” que determinam o seu encaminhamento, sem necessidade de examinar o conteúdo dos próprios pacotes. Isto permite a criação de circuitos “extremo-aextremo” através de qualquer tipo de rede de transporte e independentemente do protocolo de encaminhamento que é utilizado. O projecto do MPLS considera múltiplas tecnologias no sentido de prestar um serviço único de transporte de dados, tentando simultaneamente proporcionar capacidades de engenharia de tráfego e controlo “out-of-band”, uma característica muito atraente para uma implementação em grande escala. No fundo, o MPLS é uma forma de consolidar muitas redes IP dentro de uma única rede. Dada a importância desta tecnologia, é urgente desenvolver ferramentas que permitam entender melhor a sua complexidade. O MPLS corre normalmente nas redes de núcleo dos ISPs. No sentido de tornar o seu estudo viável, recorreu-se nesta dissertação à emulação para implementar cenários de complexidade adequada. Existem actualmente boas ferramentas disponíveis que permitem a recriação em laboratório de cenários bastante complicados. Contudo, a exigência computacional da emulação é proporcional à complexidade do projecto em questão, tornando-se rapidamente impossível de realizar numa única máquina. A computação distribuída ou a “Cloud Computing” são actualmente as abordagens mais adequadas e inovadoras apara a resolução deste problema. Esta dissertação tem como objectivo criar algumas experiências em laboratório que evidenciam aspectos relevantes da tecnologia MPLS, usando para esse efeito um emulador computacional, o Dynamips, impulsionado por generosas fontes computacionais disponibilizadas pela Amazon ec2. A utilização destas ferramentas de emulação permite testar cenários de rede e serviços reais em ambiente controlado, efectuando o debugging das suas configurações e optimizando o seu desempenho, antes de os colocar em funcionamento nas redes em operação.The Multiprotocol Label Switching (MPLS) is a highly scalable and agnostic protocol to carry network data. Operating at "Layer 2.5" of the OSI model, MPLS is an highperformance mechanism that is used at the network backbone for conveying data from one network node to the next. The success of MPLS results from the fact that it enables the network to carry all kinds of traffic, ranging from IP to layer 2 traffic, since it encapsulates the packets of the diverse network protocols, allowing the creation of "virtual links" between distant nodes. MPLS belongs to the family of packet switched networks, where labels are assigned to data packets that are forwarded based on decisions that rely only on the label contents, without the need to examine the packets contents. This allows the creation of end-to-end circuits across any type of transport medium, using any protocol. The MPLS design takes multiform transport technologies into account to provide a unified data-carrying service, attempting simultaneously to preserve traffic engineering and out-of-band control, a very attractive characteristic for large-scale deployment. MPLS is the way to consolidate many IP networks into a single one. Due to this obvious potential, it is urgent to develop means and tools to better understand its functioning and complexity. MPLS normally runs at the backbone of Service Providers networks, being deployed across an extensive set of expensive equipment. In order to turn the study of MPLS feasible, emulation was considered as the best solution. Currently, there are very good available tools to recreate, in a lab environment, quite complicated scenarios. However, the computational demand of the emulation is proportional to the complexity of the project, becoming quickly unfeasible in a single machine. Fortunately, distributed computing or Cloud computing are suitable and novel approaches to solve this computation problem. So, this work aims to create some lab experiments that can illustrate/demonstrate relevant aspects of the MPLS technology, using the Dynamips emulator driven by the computational resources that were made available by the Amazon ec2 cloud computing facilities. The utilization of these emulation tools allows testing real networks and service scenarios in a controlled environment, being able to debug their configurations and optimize their performance before deploying them in real operating networks

Fecal microbiota and bile acid interactions with systemic and adipose tissue metabolism in diet-induced weight loss of obese postmenopausal women

Microbiota and bile acids in the gastrointestinal tract profoundly alter systemic metabolic processes. In obese subjects, gradual weight loss ameliorates adipose tissue inflammation and related systemic changes. We assessed how rapid weight loss due to a very low calorie diet (VLCD) affects the fecal microbiome and fecal bile acid composition, and their interactions with the plasma metabolome and subcutaneous adipose tissue inflammation in obesity. We performed a prospective cohort study of VLCD-induced weight loss of 10% in ten grades 2-3 obese postmenopausal women in a metabolic unit. Baseline and post weight loss evaluation included fasting plasma analyzed by mass spectrometry, adipose tissue transcription by RNA sequencing, stool 16S rRNA sequencing for fecal microbiota, fecal bile acids by mass spectrometry, and urinary metabolic phenotyping by H-NMR spectroscopy. Outcome measures included mixed model correlations between changes in fecal microbiota and bile acid composition with changes in plasma metabolite and adipose tissue gene expression pathways. Alterations in the urinary metabolic phenotype following VLCD-induced weight loss were consistent with starvation ketosis, protein sparing, and disruptions to the functional status of the gut microbiota. We show that the core microbiome was preserved during VLCD-induced weight loss, but with changes in several groups of bacterial taxa with functional implications. UniFrac analysis showed overall parallel shifts in community structure, corresponding to reduced abundance of the genus Roseburia and increased Christensenellaceae;g__ (unknown genus). Imputed microbial functions showed changes in fat and carbohydrate metabolism. A significant fall in fecal total bile acid concentration and reduced deconjugation and 7-α-dihydroxylation were accompanied by significant changes in several bacterial taxa. Individual bile acids in feces correlated with amino acid, purine, and lipid metabolic pathways in plasma. Furthermore, several fecal bile acids and bacterial species correlated with altered gene expression pathways in adipose tissue. VLCD dietary intervention in obese women changed the composition of several fecal microbial populations while preserving the core fecal microbiome. Changes in individual microbial taxa and their functions correlated with variations in the plasma metabolome, fecal bile acid composition, and adipose tissue transcriptome

Systematic review of the effects of the intestinal microbiota on selected nutrients and non-nutrients

The systematic review demonstrates that the IM plays a major role in the breakdown and transformation of the dietary substrates examined. However, recent human data are limited with the exception of data from studies examining fibres and polyphenols. Results observed in relation with dietary substrates were not always consistent or coherent across studies and methodological limitations and differences in IM analyses made comparisons difficult. Moreover, non-digestible components likely to reach the colon are often not well defined or characterised in studies making comparisons between studies difficult if not impossible. Going forward, further rigorously controlled randomised human trials with well-defined dietary substrates and utilizing omic-based technologies to characterise and measure the IM and their functional activities will advance the field. Current evidence suggests that more detailed knowledge of the metabolic activities and interactions of the IM hold considerable promise in relation with host health

Analysis of the intestinal microbiota using SOLiD 16S rRNA gene sequencing and SOLiD shotgun sequencing

Background: Metagenomics seeks to understand microbial communities and assemblages by DNA sequencing. Technological advances in next generation sequencing technologies are fuelling a rapid growth in the number and scope of projects aiming to analyze complex microbial environments such as marine, soil or the gut. Recent improvements in longer read lengths and paired-sequencing allow better resolution in profiling microbial communities. While both 454 sequencing and Illumina sequencing have been used in numerous metagenomic studies, SOLiD sequencing is not commonly used in this area, as it is believed to be more suitable in the context of reference-guided projects. Results: To investigate the performance of SOLiD sequencing in a metagenomic context, we compared taxonomic profiles of SOLiD mate-pair sequencing reads with Sanger paired reads and 454 single reads. All sequences were obtained from the bacterial 16S rRNA gene, which was amplified from microbial DNA extracted from a human fecal sample. Additionally, from the same fecal sample, complete genomic microbial DNA was extracted and shotgun sequenced using SOLiD sequencing to study the composition of the intestinal microbiota and the existing microbial metabolism. We found that the microbiota composition of 16S rRNA gene sequences obtained using Sanger, 454 and SOLiD sequencing provide results comparable to the result based on shotgun sequencing. Moreover, with SOLiD sequences we obtained more resolution down to the species level. In addition, the shotgun data allowed us to determine a functional profile using the databases SEED and KEGG. Conclusions: This study shows that SOLiD mate-pair sequencing is a viable and cost-efficient option for analyzing a complex microbiome. To the best of our knowledge, this is the first time that SOLiD sequencing has been used in a human sample. Keywords: Metagenomics; Intestinal Microbiota; Next-Generation Sequencing; SOLiD Mate-Pair Sequencing; Human Fecal SamplePublished versio

A Comparative Study on the WCRF International/University of Bristol Methodology for Systematic Reviews of Mechanisms Underpinning Exposure-Cancer Associations

The World Cancer Research Fund (WCRF) International and the University of Bristol have developed a novel framework for providing an overview of mechanistic pathways and conducting a systematic literature review of the biologically plausible mechanisms underlying exposure-cancer associations. Two teams independently applied the two-stage framework on mechanisms underpinning the association between body fatness and breast cancer to test the framework feasibility and reproducibility as part of a WCRF-commissioned validation study. In stage I, a "hypothesis-free" approach was used to provide an overview of potential intermediate mechanisms between body fatness and breast cancer. Dissimilar rankings of potential mechanisms were observed between the two teams due to different applications of the framework. In stage II, a systematic review was conducted on the insulin-like growth factor 1 receptor (IGF1R) chosen as an intermediate mechanism. Although the studies included differed, both teams found inconclusive evidence for the body fatness-IGF1R association and modest evidence linking IGF1R to breast cancer, and therefore concluded that there is currently weak evidence for IGF1R as mechanism linking body fatness to breast cancer. The framework is a good starting point for conducting systematic reviews by integrating evidence from mechanistic studies on exposure-cancer associations. On the basis of our experience, we provide recommendations for future users. (C) 2017 AACR

Corticosteroids as adjuvant therapy for ocular toxoplasmosis.

BACKGROUND: Ocular infestation with Toxoplasma gondii, a parasite, may result in inflammation in the retina, choroid, and uvea and consequently lead to complications such as glaucoma, cataract, and posterior synechiae. OBJECTIVES: The objective of this systematic review was to assess the effects of adjunctive use of corticosteroids for ocular toxoplasmosis. SEARCH METHODS: We searched CENTRAL (which contains the Cochrane Eyes and Vision Group Trials Register) (The Cochrane Library 2012, Issue 9), Ovid MEDLINE, Ovid MEDLINE In-Process and Other Non-Indexed Citations, Ovid MEDLINE Daily, Ovid OLDMEDLINE, (January 1950 to October 2012), EMBASE (January 1980 to October 2012), Latin American and Caribbean Literature on Health Sciences (LILACS) (January 1982 to October 2012), the metaRegister of Controlled Trials (mRCT) (www.controlled-trials.com), ClinicalTrials.gov (www.clinicaltrials.gov) and the WHO International Clinical Trials Registry Platform (ICTRP) (www.who.int/ictrp/search/en). We searched the reference lists of included studies for any additional studies not identified by the electronic searches. We did not use any date or language restrictions in the electronic searches for trials. We last searched the electronic databases on 11 October 2012. SELECTION CRITERIA: We planned to include randomized and quasi-randomized controlled trials. Eligible trials would have enrolled participants of any age who were immunocompetent and were diagnosed with active ocular toxoplasmosis. Included trials would have compared anti-parasitic therapy plus corticosteroids versus anti-parasitic therapy alone, or different doses or times of initiation of corticosteroids. DATA COLLECTION AND ANALYSIS: Two authors independently screened titles and abstracts retrieved from the electronic searches. We retrieved full-text articles of studies categorized as \u27unsure\u27 or \u27include\u27 after review of the abstracts. Two authors independently reviewed each full-text article. Discrepancies were resolved through discussion. MAIN RESULTS: The electronic searches retrieved 368 titles and abstracts. We reviewed 20 full-text articles. We identified no trials eligible for inclusion in this systematic review. AUTHORS\u27 CONCLUSIONS: Although research has identified wide variation in practices regarding use of corticosteroids, our systematic review did not identify evidence from randomized controlled trials for the role of corticosteroids in the management of ocular toxoplasmosis. Several questions remain unanswered by well-conducted randomized trials in this context, including whether use of corticosteroids is more effective than use of anti-parasitic therapy alone, when corticosteroids should be initiated in the treatment regimen (early versus late course of treatment), and which dosage and duration of steroid use is best. These questions are easily amenable to research using a randomized controlled design and they are ethical due to the absence of evidence to support or discourage use of corticosteroids for this condition. The question of foremost importance, however, is whether they should be used as adjunct therapy (that is, additional) to anti-parasitic agents