MMP-13 binds to platelet receptors αIIbβ3 and GPVI and impairs aggregation and thrombus formation.

BACKGROUND: Acute thrombotic syndromes lead to atherosclerotic plaque rupture with subsequent thrombus formation, myocardial infarction and stroke. Following rupture, flowing blood is exposed to plaque components, including collagen, which triggers platelet activation and aggregation. However, plaque rupture releases other components into the surrounding vessel which have the potential to influence platelet function and thrombus formation. OBJECTIVES: Here we sought to elucidate whether matrix metalloproteinase-13 (MMP-13), a collagenolytic metalloproteinase up-regulated in atherothrombotic and inflammatory conditions, affects platelet aggregation and thrombus formation. RESULTS: We demonstrate that MMP-13 is able to bind to platelet receptors alphaIIbbeta3 (αIIbβ3) and platelet glycoprotein (GP)VI. The interactions between MMP-13, GPVI and αIIbβ3 are sufficient to significantly inhibit washed platelet aggregation and decrease thrombus formation on fibrillar collagen. CONCLUSIONS: Our data demonstrate a role for MMP-13 in the inhibition of both platelet aggregation and thrombus formation in whole flowing blood, and may provide new avenues of research into the mechanisms underlying the subtle role of MMP-13 in atherothrombotic pathologies

Low Flow Testing

In arid environments, streamflow enhancement in low flow channels is common. It is challenging to assess enhancement project effectiveness in these channels because of uncertainties in metering (regarding both meters and procedures). The basic equation for flow is: Q = VA (Eq. 1) Where, Q = channel flow rate (e.g., cubic feet per second, CFS) V = cross sectional average velocity (e.g., feet per second, fps) A = cross sectional area (e.g., square feet, ft2) There are three main factors that impact flow measurement uncertainty in natural channels: 1. Flow meter velocity measurements (V) 2. Depth and area measurements (A) 3. Lack of continuous measurements over time Items 1 and 2 above are directly shown in Eq. 1. However, the third point is important to also understand because point flow measurements are difficult to utilize, since flow changes over time. The work outlined in this report only focuses on the first two factors (V and D). The last factor is typically overcome by somehow continuously measuring the flow or relating point flow measurements to water depth (stage discharge relationship). The objectives of this study were to evaluate several meters under laboratory and semi-laboratory conditions in order to: 1. Determine the most effective meter to use under low flow conditions 2. Identify constraints in the metering process or with meters themselves 3. Utilize the results to identify improved methods or recommendations for improving low flow measurement accurac

Is faster still better in therapeutic hypothermia?

The rapid institution of therapeutic hypothermia after cardiac arrest has become an accepted practice. In the previous issue of Critical Care, Haugk and colleagues present a retrospective analysis of 13 years of experience with therapeutic hypothermia at their center that suggests an association between rate of cooling and less favorable neurological outcomes. The association most likely reflects easier cooling in patients more severely brain injured by their initial cardiac arrest, and should not lead clinicians to abandon or slow their efforts to achieve post-resuscitative cooling

Grass Referenced Based Vegetation Coefficients for Estimating Evapostranspiration for a Variery of Natural Vegetation

In arid and semi-arid regions, evapotranspiration from vegetation results in the significant utilization of available water. Accurate estimates of evapotranspiration are required for surface and subsurface hydrologic evaluations as well as irrigation district water balance studies. A significant amount of transferable information exists for irrigated agricultural crops through past and current research in the form of grass or alfalfa reference based crop coefficients (Kc) and basal crop coefficients (Kcb). However, transferable evapotranspiration information on natural vegetation is limited. Much of the work was conducted in the early to mid-1900’s and is presented as actual evapotranspiration from the vegetation at the research site either as annual or monthly values. In some cases, the data may have been referenced to evaporation pan measurements (typically Class A type pans) with unknown site conditions. An intensive literature review was conducted to extract monthly measured evapotranspiration information for natural vegetation types under various conditions. Monthly vegetation coefficients (Kv) for standardized grass reference based evapotranspiration (ETo) were computed using long-term average grass reference evapotranspiration information computed with data from nearby weather stations. Comparisons of the Kv values for similar vegetation indicate higher variability during the non-summer months but results from most of the studies examined are in good agreement. These Kv values provide some level of transferability so that it is possible to compute an accurate estimate of vegetative evapotranspiration with daily or monthly standardized grass reference evapotranspiration values in areas away from the original study

Pupillometry as a tool to study expertise in medicine

Background Pupillometry has been studied as a physiological marker for quantifying cognitive load since the early 1960s. It has been established that small changes in pupillary size can provide an index of the cognitive load of a participant as he/she performs a mental task. The utility of pupillometry as a measure of expertise is less well established, although recent research in the fields of education, medicine and psychology indicates that differences in pupillary size during domain-specific tasks allows differentiation between experts and novices in appropriately designed experiments.Purpose The goal of this review is to explore the existing body of evidence for the use of pupillometry as a measure of expertise and to identify its strengths and constraints within the context of expertise research in the medical sciences.Results Pupillometry is a robust metric that allows researchers to better understand cognitive load in medical practitioners with varying levels of expertise. In medical expertise research, it has been used to study surgeons, anesthetists and emergency physicians. Its strengths include its ability to provide quantitative and objective outputs, to be measured unobtrusively with new technology and to be precisely computed as cognitive load changes over the course of completion of a task. Constraints associated with this methodology include its potential inaccuracy with changes in ambient light and pupillary accommodation as well as the need for relatively expensive equipment. Conclusion With recent technological advances, pupillometry has become a simple and robust method for quantifying physiological changes attributable to cognitive load and is increasingly being utilized in medical education. It can be used as a reliable marker of mental effort and has been shown to differentiate levels of expertise in medical practitioners

Infrared Inspection

Infrared thermography is the use of infrared radiation to qualitatively and quantitatively express heat signatures and heat differences. Infrared inspection can help identify weak connections in an electrical box, uneven heating of pump windings, overheating of bearings, and many other possibly devastating problems well before any failure occurs. The use of thermography as a preventative tool can increase system reliability and efficiency

California Energy Commission Agricultural Peak Load Reduction Program Case Study: Berrenda Mesa WSD

Berrenda Mesa Water Storage District (BMWSD) is located in the southern part of the San Joaquin Valley near Bakersfield. The district receives water from the State Water Project – California Aqueduct

Rating Rectangular Farm Delivery Meter Gates for Flow Measurement

Traditional meter gates for farm delivery flow measurement from an open channel conveyance have traditionally incorporated round canal gates (Armco type) for control. In recent years, some irrigation water agencies (i.e., irrigation districts) have replaced deteriorating round gates with lower-cost rectangular gates that cover round holes. Similar to the situation described in a companion paper, where round gates were examined, there have been no investigations into flow measurement uncertainty using the existing rating tables for these gates. In this study, two commonly used rectangular gate sizes, 0.46 m (18-in.) and 0.61 m (24-in.), were tested under scenarios of various gate openings, upstream heads, and head differences. Coefficient of discharge (Cd) values were computed based on actual gate open areas. These improved Cdvalues were used to generate new discharge rating tables for 0.46 m (18-in.) and 0.61 m (24-in.) rectangular meter gates. Limitations for these rectangular gates are discussed. If guidelines presented in this paper and in the companion paper are followed, the average instantaneous flow measurement uncertainty that could be expected is better than ±5%. However, uncertainty is higher (up to approximately ±9.5%) at the lower end of the recommended gate openings [0.10 m (4 in.)] for these rectangular gates