A study of the effects of exercise on the urinary metabolome using normalisation to individual metabolic output

Aerobic exercise, in spite of its multi-organ benefit and potent effect on the metabolome, has yet to be investigated comprehensively via an untargeted metabolomics technology. We conducted an exploratory untargeted liquid chromatography mass spectrometry study to investigate the effects of a one-h aerobic exercise session in the urine of three physically active males. Individual urine samples were collected over a 37-h protocol (two pre-exercise and eight post-exercise). Raw data were subjected to a variety of normalization techniques, with the most effective measure dividing each metabolite by the sum response of that metabolite for each individual across the 37-h protocol expressed as a percentage. This allowed the metabolite responses to be plotted on a normalised scale. Our results highlight significant metabolites located in the following systems: purine pathway, tryptophan metabolism, carnitine metabolism, cortisol metabolism, androgen metabolism, amino acid oxidation, as well as metabolites from the gastrointestinal microbiome. Many of the significant changes observed in our pilot investigation mirror previous research studies, of various methodological designs, published within the last 15 years, although they have never been reported at the same time in a single study

An XML format for benchmarks in High School Timetabling

The High School Timetabling Problem is amongst the most widely used timetabling problems. This problem has varying structures in different high schools even within the same country or educational system. Due to lack of standard benchmarks and data formats this problem has been studied less than other timetabling problems in the literature. In this paper we describe the High School Timetabling Problem in several countries in order to find a common set of constraints and objectives. Our main goal is to provide exchangeable benchmarks for this problem. To achieve this we propose a standard data format suitable for different countries and educational systems, defined by an XML schema. The schema and datasets are available online

Σεισμική συμπεριφορά ξύλινων διαφραγμάτων σε κτίρια από άοπλη τοιχοποιία

Faced with an expected general labor shortage, Saudi Arabia allowed foreign workers to enter its labor markets or to be recruited by employers on a large scale since 1973. This led to a continuous inflow of labor migration, so much so that as early as 1980 there were more employed immigrants than natives. In this dissertation the Saudi policy to meet labor shortages with expatriate workers is evaluated using empirical analysis. Specifically, this study consists of: (1) a comparative study of other countries\u27 policies on labor shortages and immigration and the implications for the Saudi case; (2) a critical assessment of the effects of immigration on domestic wages supplemented by an empirical analysis of the Saudi case; and (3) an empirical analysis of the elasticities of substitution between native and expatriate workers in Saudi production, in which we also consider the effects of immigration on the level of wages. First, our study of other countries\u27 experiences with the use of foreign workers on a temporary basis clearly indicates that this can be a misleading strategy to counter labor shortages. The main reason is that several factors usually intervene and make the presence of foreign workers more of a permanent one. Most noticeable is the possibility that immigration is a cumulative and self-reinforcing phenomenon. The evidence from the Saudi case shows that it is not immune from this pitfall. Second, when we considered the effects of immigration on domestic wages, the evidence shows that, under almost any assumptions immigrants will reduce wage rates. The empirical analysis of the Saudi case shows that expatriate workers did reduce wage growth in the private sector. This effect seems to be, however, rather small and uneven among different labor groups. Our results show that had there been no immigration, wage growth could have been 3-10 percent higher than it was between 1968 and 1973 and 1-12 percent higher than it was betwen 1978 and 1982. Third, our study includes estimates of the elasticities of complementarity between native and immigrant labor. The elasticity of substitution between natives and migrants is estimated to be -2.13. This value changes when labor is disaggregated by skill or educational levels, but in all the classifications migrants and natives were found to be substitutable. This means that an increase in immigrant employment will decrease native employment. The simulation exercise which we used confirmed this conclusion

The application of metabolomic profiling to the effects of physical activity

Physical activity and exercise are potent lifestyle interventions for the reduction of several psychosomatic risks, including cardiovascular disease, diabetes and mild-to-moderate depression. The current review describes the studies which have utilised a metabolomics/ metabolite profiling approach in combination with exercise in the period between 1999-2014 in predominantly healthy subjects (N:64) and in a smaller subset of patient cohorts (N:10) between 2011-2014. A broad range of intervention types were used in both healthy and patient cohorts with varying exercise regimes with or without supplement use. Effects on purine metabolism, which have been known for over 40 years, were by far the most well-cited and persistent finding. Other reported effects included observations of: glucocorticoid and androgen metabolism, glycolysis and Krebs cycle, gluconeogenesis, fatty acid metabolism, fatty acid oxidation and pantothenic acid, amino acid oxidation, tryptophan metabolism and effects on microbiome metabolites. The discussion section draws observations from the very disparate studies together and links them to the general aspects of human metabolism

Tsunami hazards in the Eastern Mediterranean: strong earthquakes and tsunamis in the East Hellenic Arc and Trench system

International audienceData on tsunami phenomena occurring in the East Hellenic Arc and Trench system (HA-T) from antiquity up to the present have been updated, critically evaluated and compiled in the standard GITEC format developed in the last decade for the New European Tsunami Catalogue. New field observations are presented for the tsunamis of 9 February 1948 and 24 March 2002. From the 18 tsunamis reported eight are rather well-documented while another nine remain doubtful. The mean recurrence of strong tsunamis is likely equal to about 142 years. Most of the tsunamis documented are caused by strong earthquakes occurring in the area offshore Rhodes to the east or northeast of the island. However, there are large earthquakes near Rhodes that do not cause tsunamis, like the 1926 and 1957 ones, which is of particular importance for the tsunami hazard assessment

A study of the effects of exercise on the urinary metabolome using normalisation to individual metabolic output

Aerobic exercise, in spite of its multi-organ benefit and potent effect on the metabolome, has yet to be investigated comprehensively via an untargeted metabolomics technology. We conducted an exploratory untargeted liquid chromatography mass spectrometry study to investigate the effects of a one-h aerobic exercise session in the urine of three physically active males. Individual urine samples were collected over a 37-h protocol (two pre-exercise and eight post-exercise). Raw data were subjected to a variety of normalization techniques, with the most effective measure dividing each metabolite by the sum response of that metabolite for each individual across the 37-h protocol expressed as a percentage. This allowed the metabolite responses to be plotted on a normalised scale. Our results highlight significant metabolites located in the following systems: purine pathway, tryptophan metabolism, carnitine metabolism, cortisol metabolism, androgen metabolism, amino acid oxidation, as well as metabolites from the gastrointestinal microbiome. Many of the significant changes observed in our pilot investigation mirror previous research studies, of various methodological designs, published within the last 15 years, although they have never been reported at the same time in a single study

Fano resonances in THz metamaterials composed of continuous metallic wires and split ring resonators

Cataloged from PDF version of article.We demonstrate theoretically and experimentally that Fano resonances can be obtained in terahertz metamaterials that are composed of periodic continuous metallic wires dressed with periodic split ring resonators. An asymmetric Fano lineshape has been found in a narrow frequency range of the transmission curve. By using a transmission line combined with lumped element model, we are able to not only fit the transmission spectra of Fano resonance which is attributed to the coupling and interference between the transmission continuum of continuous metallic wires and the bright resonant mode of split ring resonators, but also reveal the capacitance change of the split ring resonators induced frequency shift of the Fano resonance. Therefore, the proposed theoretical model shows more capabilities than conventional coupled oscillator model in the design of Fano structures. The effective parameters of group refractive index of the Fano structure are retrieved, and a large group index more than 800 is obtained at the Fano resonance, which could be used for slow light devices. (C) 2014 Optical Society of Americ

Metabolomic profiling of submaximal exercise at a standardised relative intensity in healthy adults

Ten physically active subjects underwent two cycling exercise trials. In the first, aerobic capacity (VO2max) was determined and the second was a 45 min submaximal exercise test. Urine samples were collected separately the day before (day 1) , the day of (day 2), and the day after (day 3) the submaximal exercise test (12 samples per subject). Metabolomic profiling of the samples was carried out using hydrophilic interaction chromatography (HILIC) coupled to an Orbitrap Exactive mass spectrometer. Data were extracted, database searched and then subjected to principle components (PCA) and orthogonal partial least squares (OPLSDA) modelling. The best results were obtained from pre-treating the data by normalising the metabolites to their mean output on days 1 and 2 of the trial. This allowed PCA to separate the day 2 first void samples (D2S1) from the day 2 post-exercise samples (D2S3) PCA also separated the equivalent samples obtained on day 1 (D1S1 and D1S3). OPLSDA modelling separated both the D2S1 and D2S3 samples and D1S1 and D1S3 samples. The metabolites affected by the exercise samples included a range of purine metabolites and several acyl carnitines. Some metabolites were subject to diurnal variation these included bile acids and several amino acids, the variation of these metabolites was similar on day 1 and day 2 despite the exercise intervention on day 2. Using OPLS modelling it proved possible to identify a single abundant urinary metabolite provisionally identified as oxo-aminohexanoic acid (OHA) as being strongly correlated with VO2max when the levels in the D2S3 samples were considered