Proteomics for rejection diagnosis in renal transplant patients: where are we now?

Rejection is one of the key factors that determine the long-term allograft function and survival in renal transplant patients. Reliable and timely diagnosis is important to treat rejection as early as possible. Allograft biopsies are not suitable for continuous monitoring of rejection. Thus, there is an unmet need for non-invasive methods to diagnose acute and chronic rejection. Proteomics in urine and blood samples has been explored for this purpose in 29 studies conducted since 2003. This review describes the different proteomic approaches and summarizes the results from the studies that examined proteomics for the rejection diagnoses. The potential limitations and open questions in establishing proteomic markers for rejection are discussed, including ongoing trials and future challenges to this topic

Formulating earthquake response modes in Iran

Thesis (M. Eng.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2004.Includes bibliographical references (leaves 50-51).The objective of this paper was to try and find the optimal distribution of rescuers after an earthquake with a very large magnitude caused major damage in two different cities. A model was developed to optimally divide all of the available rescuer workers such that the expected number of lives saved was maximized. When the method was tested on random sets of data on average a 5% improvement in lives saved was found. However it was also determined that there was a positive relationship between percent improvement and severity of the earthquake. This shows that the method is especially effective when extreme amounts of damage occur.by Michael D. Metzger.M.Eng

Using S Isotopes to Identify the Source of Gypsum in Mammoth Cave

Many of the dry passages of the cave are lined with gypsum (CaSO4�2H2O) crystals, an evaporite mineral. However, the source of the sulfur in these gypsum deposits is poorly constrained with possible sources including pyrite, sedimentary gypsum/anhydrite, and carbonate associated sulfate (“CAS”, SO42- substituted for CO3 2- in the calcite crystal lattice). The two most abundant forms of sulfur in the bedrock above and around Mammoth Cave are pyrite (FeSs) and CAS. These phases commonly have very different isotopic signatures (δ34S)* and the δ34S values of these phases can be compared to the δ34S of the gypsum to aid in identifying the source of the sulfur. Isolation of sulfur from pyrite and CAS is currently ongoing. Results from 110 gypsum crystals, 4 rocks in strata from within the caves, and 15 rocks from strata overlying the caves reveal some distinct patterns. 1) Gypsum crystals show relatively small scatter (~5‰) for samples from a single location (e.g. a 30 m2 room). 2) A significant correlation between δ34Sgypsum and elevation suggests a variable δ34Ssource over vertical distances of a few meters. 3) Microsampling of sulfur along the growth axes of single gypsum crystals shows a constant δ34S values suggesting no change in δ34S of the S source during its growth. Because the growth period of these crystals may be on the order of thousands of years, these results suggest a constant sulfate for long intervals. The relationship to δ34S of samples in a given room and elevation suggests that the source of gypsum sulfur is local, arising from lateral, rather than vertical, fluid fl ow, an important insight into the transport pathways of water in a karstic system. Sampling of pyrite and CAS is currently ongoing. *δ34S = [(34S/32Ssample)/(34S/32Sstandard)-1(x)1,000‰ where 34S and 32S are the molar ratios of each S isotope given in “per mil” (‰), equivalent to parts per thousand

Third International Workshop on Variability Modelling of Software-intensive Systems. Proceedings

This ICB Research Report constitutes the proceedings of the Third International Workshop on Variability Modelling of Software-intensive Systems (VaMoS'09), which was held from January 28-30, 2009 at the University of Sevilla, Spain

Use of severity classification systems in the surgical decision-making process in emergency laparotomy for perforated diverticulitis

Purpose: Hartman's procedure (HP) or primary anastomosis (PA) are the two surgical techniques used in patients undergoing emergency colectomy for perforated diverticulitis. There are no objective criteria to guide the surgeon's choice of procedure. This study assesses whether classification and scoring systems can be used in the decision-making process. Methods: One hundred eleven patients undergoing emergency laparotomy for perforated diverticulitis were analyzed. Logistic regression and interaction models were used to determine the predictive value in the two settings. Results: Sixty five patients underwent HP and 46 patients underwent PA. Patients with HP had significantly higher scores, median age, and were more often on immunosuppressive medication. Mortality and surgical morbidity did not differ between the groups. The clinical anastomotic leak rate was 28.3% in the PA group. In the univariate logistic regression for in-hospital death, all scores showed a significant influence. The multivariate logistic regression analysis showed that only Charlson comorbidity index (CCI) and American Society of Anesthesiologists score had a significant influence on mortality. Each score was analyzed for its predictive value regarding mortality and morbidity with respect to type of operative procedure. Only CCI revealed a trend towards statistical significance. The risk of death increases with increasing CCI when PA is performed compared to HP. Conclusion: None of the tested scores can be used to help the surgeon decide whether a PA or HP is appropriate in a specific patient. Comorbidity, represented as CCI in this study, might be more important than the locoregional situatio

An X-ray measurement of Titan's atmospheric extent from its transit of the Crab Nebula

Saturn's largest satellite, Titan, transited the Crab Nebula on 5 January 2003. We observed this astronomical event with the {\it Chandra} X-ray Observatory. An ``occultation shadow'' has clearly been detected and is found to be larger than the diameter of Titan's solid surface. The difference gives a thickness for Titan's atmosphere of 880 $\pm$ 60 km. This is the first measurement of Titan's atmospheric extent at X-ray wavelengths. The value measured is consistent with or slightly larger than those estimated from earlier Voyager observations at other wavelengths. We discuss the possibility of temporal variations in the thickness of Titan's atmosphere.Comment: 14 pages, 5 figures, AASTeX preprint. Accepted for publication in the Astrophysical Journa

Optical trapping with "on-demand" two-photon luminescence using Cr:LiSAF laser with optically addressed saturable Bragg reflector

We demonstrate a diode-pumped Cr:LiSAF laser with controllable and reliable fast switching between its continuous-wave and mode-locked states of operation using an optically-addressed semiconductor Bragg reflector, permitting dyed microspheres to be continuously trapped and monitored using a standard microscope imaging and on-demand two-photon-excited luminescence techniques

XROMM analysis of tooth occlusion and temporomandibular joint kinematics during feeding in juvenile miniature pigs

Like humans, domestic pigs are omnivorous and thus are a common model for human masticatory function. Prior attempts to characterize food–tooth interactions and jaw movements associated with mastication have been limited to aspects of the oral apparatus that are visible externally (with videography) and/or to 2D movements of oral structures (with monoplanar videofluoroscopy). We used XROMM, a 3D technique that combines CT-based morphology with biplanar videofluoroscopy, to quantify mandibular kinematics, tooth occlusion and mandibular condylar displacements within the temporomandibular joint (TMJ) during feeding. We observed that the pig TMJ moved detectably in only three of six possible degrees of freedom during mastication: two rotations, pitch and yaw; and one translation, protraction–retraction. Asymmetrical yaw around a dorsoventral axis produced the observed alternating left–right chewing cycles responsible for food reduction. Furthermore, the relative motions of the upper and lower premolars contained a substantial mesiodistal component in addition to the buccolingual component, resulting in an oblique (rather than a strictly transverse) power stroke. This research demonstrates the capacity of XROMM to explore the kinematic underpinnings of key masticatory movements, such as the occlusal power stroke, by integrating tooth, joint and rigid body jaw movements. XROMM also allowed us to test kinematic hypotheses based on skeletal anatomy with actual kinematics observed during naturalistic feeding behaviors. We observed that the soft tissue structures of the TMJ appear to play a significant role in limiting the range of motion of a joint, and thus analyses based solely on osseous morphology may over-estimate joint mobility