Spatial variability of climate and past atmospheric circulation patterns from central West Antarctic glaciochemistry

Atmospheric circulation patterns and the spatial variability of atmospheric chemistry and moisture transport in central West Antarctica are investigated using new 40 year long (1954–1994 A.D.) glaciochemical and accumulation rate records developed from four firn cores from this region. The core sites lie on a 200 km traverse from 82° 22′ S, 119° 17′ W to 81° 22′ S, 107° 17′ W. The glaciochemical records represent the major ionic species present in Antarctic snow: Na+, K+, Mg2+, Ca2+, Cl−, NO3−, and SO42−. High spatial variability appears in comparisons of full record averages and poor intersite linear correlation results. Accumulation rates show 50–100% changes over distances of 50–100 km and sea‐salt concentrations drop by 50% between the middle two sites. One likely contributor to the high variability seen at this spatial scale is variability in synoptic‐ and finer‐scale meteorology. Empirical orthogonal function (EOF) analysis shows that 80% or more of the variance in site chemistry can be attributed to two types of air masses: winter season air (50–70% of site variance) with a strong marine signature (heavy loading of sea‐salt species) and summer season air (21% of the variance), marked by marine biogenic non‐sea‐salt SO4 plus NO3. This pattern of winter and summer regimes appears at other West Antarctic sites suggesting it may apply to the entire region. We show that a general picture of the patterns of variability in West Antarctica can best be drawn by using an analysis technique that fully exploits high resolution, multiparameter, multisite data sets

Oral diabetes medication monotherapy and short-term mortality in individuals with type 2 diabetes and coronary artery disease

Objective To determine whether sulfonylurea use, compared with non-sulfonylurea oral diabetes medication use, was associated with 2-year mortality in individuals with well-controlled diabetes and coronary artery disease (CAD). Research design and methods We studied 5352 US veterans with type 2 diabetes, obstructive CAD on coronary angiography, hemoglobin A1c ≤7.5% at the time of catheterization, and taking zero or one oral diabetes medication (categorized as no medications, non-sulfonylurea medication, or sulfonylurea). We estimated the association between medication category and 2-year mortality using inverse probability of treatment-weighted (IPW) standardized mortality differences and IPW multivariable Cox proportional hazards regression. Results 49%, 35%, and 16% of the participants were on no diabetes medications, non-sulfonylurea medications, and sulfonylureas, respectively. In individuals on no medications, non-sulfonylurea medications, and sulfonylureas, the unadjusted mortality rates were 6.6%, 5.2%, and 11.9%, respectively, and the IPW-standardized mortality rates were 5.9%, 6.5%, and 9.7%, respectively. The standardized absolute 2-year mortality difference between non-sulfonylurea and sulfonylurea groups was 3.2% (95% CI 0.7 to 5.7) (p=0.01). In Cox proportional hazards models, the point estimate suggested that sulfonylurea use might be associated with greater hazard of mortality than non-sulfonylurea medication use, but this finding was not statistically significant (HR 1.38 (95% CI 1.00 to 1.93), p=0.05). We did not observe significant mortality differences between individuals on no diabetes medications and non-sulfonylurea users. Conclusions Sulfonylurea use was common (nearly one-third of those taking medications) and was associated with increased 2-year mortality in individuals with obstructive CAD. The significance of the association between sulfonylurea use and mortality was attenuated in fully adjusted survival models. Caution with sulfonylurea use may be warranted for patients with well-controlled diabetes and CAD, and metformin or newer diabetes medications with cardiovascular safety data could be considered as alternatives when individualizing therapy

Comparative transcriptomics of stickleback immune gene responses upon infection by two helminth parasites, Diplostomum pseudospathaceum and Schistocephalus solidus

Immune systems of vertebrates are much more diverse than previously thought, in particular at the base of the vertebrate clade. RNA-seq was used to describe in detail the transcriptomic response of stickleback hosts to infection by two helminth parasites, the trematode . Diplostomum pseudospathaceum (2 genotypes plus a genotype mix) and the cestode . Schistocephalus solidus. Based on a global transcription profiling, we present immune genes that are active during chronic or multiple repeated infection. We found that the transcription profiles of . D. pseudospathaceum genotypes were as divergent as those of the two parasite species. When comparing the host immune response, only 5 immune genes were consistently upregulated upon infection by both species. These genes indicated a role for enhanced toll like receptor (TLR) activity (CTSK, CYP27B1) and an associated positive regulation of macrophages (CYP27B1, THBS1) for general helminth defense. We interpret the largely differentiated gene expression response among parasite species as general redundancy of the vertebrate immune system, which was also visible in genotype-specific responses among the different . D. . pseudospathaceum infections. The present study provides the first evidence that IL4-mediated activation of T-helper lymphocyte cells is also important in anti-helminthic immune responses of teleost fish

North Atlantic climate variability from a self-organizing map perspective

[1] North Atlantic variability in general, and the North Atlantic Oscillation (NAO) in particular, is a long-studied, very important but still not well-understood problem in climatology. The recent trend to a higher wintertime NAO index was accompanied by an additional increase in the Azores High not coupled to changes in the Icelandic Low, as shown by a self-organizing maps (SOMs) analysis of monthly mean DJF mean sea level pressure data from 1957 to 2002. SOMs are a nonlinear tool to optimally extract a user-specified number of patterns or icons from an input data set and to uniquely relate any input data field to an icon, allowing analyses of occurrence frequencies and transitions complementary to principal component analysis (PCA). SOMs analysis of ERA-40 data finds a North Atlantic monopole roughly colocated with the mean position of the Azores High, as well as the well-known NAO dipole involving the Icelandic Low and the subtropical high. Little trend is shown in December, but the Azores High increased along with the NAO in January and February over the study interval, with implications for storminess in northwestern Europe. In short, our SOM-based analyses of winter MSLP have both confirmed prior knowledge and expanded it through the relative ease of use and power with nonlinear systems of the SOM-based approach to climatological analysis

Studying Cat (Felis catus) Diabetes: Beware of the Acromegalic Imposter

Naturally occurring diabetes mellitus (DM) is common in domestic cats (Felis catus). It has been proposed as a model for human Type 2 DM given many shared features. Small case studies demonstrate feline DM also occurs as a result of insulin resistance due to a somatotrophinoma. The current study estimates the prevalence of hypersomatotropism or acromegaly in the largest cohort of diabetic cats to date, evaluates clinical presentation and ease of recognition. Diabetic cats were screened for hypersomatotropism using serum total insulin-like growth factor-1 (IGF-1; radioimmunoassay), followed by further evaluation of a subset of cases with suggestive IGF-1 (>1000 ng/ml) through pituitary imaging and/ or histopathology. Clinicians indicated pre-test suspicion for hypersomatotropism. In total 1221 diabetic cats were screened; 319 (26.1%) demonstrated a serum IGF-1>1000 ng/ml (95% confidence interval: 23.6-28.6%). Of these cats a subset of 63 (20%) underwent pituitary imaging and 56/63 (89%) had a pituitary tumour on computed tomography; an additional three on magnetic resonance imaging and one on necropsy. These data suggest a positive predictive value of serum IGF-1 for hypersomatotropism of 95% (95% confidence interval: 90-100%), thus suggesting the overall hypersomatotropism prevalence among UK diabetic cats to be 24.8% (95% confidence interval: 21.2-28.6%). Only 24% of clinicians indicated a strong pre-test suspicion; most hypersomatotropism cats did not display typical phenotypical acromegaly signs. The current data suggest hypersomatotropism screening should be considered when studying diabetic cats and opportunities exist for comparative acromegaly research, especially in light of the many detected communalities with the human disease

Specific Gene Expression Responses to Parasite Genotypes Reveal Redundancy of Innate Immunity in Vertebrates

Vertebrate innate immunity is the first line of defense against an invading pathogen and has long been assumed to be largely unspecific with respect to parasite/pathogen species. However, recent phenotypic evidence suggests that immunogenetic variation, i.e. allelic variability in genes associated with the immune system, results in host-parasite genotype-by-genotype interactions and thus specific innate immune responses. Immunogenetic variation is common in all vertebrate taxa and this reflects an effective immunological function in complex environments. However, the underlying variability in host gene expression patterns as response of innate immunity to within-species genetic diversity of macroparasites in vertebrates is unknown. We hypothesized that intra-specific variation among parasite genotypes must be reflected in host gene expression patterns. Here we used high-throughput RNA-sequencing to examine the effect of parasite genotypes on gene expression patterns of a vertebrate host, the three-spined stickleback (Gasterosteus aculeatus). By infecting naïve fish with distinct trematode genotypes of the species Diplostomum pseudospathaceum we show that gene activity of innate immunity in three-spined sticklebacks depended on the identity of an infecting macroparasite genotype. In addition to a suite of genes indicative for a general response against the trematode we also find parasite-strain specific gene expression, in particular in the complement system genes, despite similar infection rates of single clone treatments. The observed discrepancy between infection rates and gene expression indicates the presence of alternative pathways which execute similar functions. This suggests that the innate immune system can induce redundant responses specific to parasite genotypes

Spatial Variability of Climate and Past Atmospheric Circulation Patterns from Central West Antarctic Glaciochemistry

Atmospheric circulation patterns and the spatial variability of atmospheric chemistry and moisture transport in central West Antarctica are investigated using new 40 year long (1954–1994 A.D.) glaciochemical and accumulation rate records developed from four firn cores from this region. The core sites lie on a 200 km traverse from 82° 22′ S, 119° 17′ W to 81° 22′ S, 107° 17′ W. The glaciochemical records represent the major ionic species present in Antarctic snow: Na+, K+, Mg2+, Ca2+, Cl−, NO3−, and SO42−. High spatial variability appears in comparisons of full record averages and poor intersite linear correlation results. Accumulation rates show 50–100% changes over distances of 50–100 km and sea-salt concentrations drop by 50% between the middle two sites. One likely contributor to the high variability seen at this spatial scale is variability in synoptic- and finer-scale meteorology. Empirical orthogonal function (EOF) analysis shows that 80% or more of the variance in site chemistry can be attributed to two types of air masses: winter season air (50–70% of site variance) with a strong marine signature (heavy loading of sea-salt species) and summer season air (21% of the variance), marked by marine biogenic non-sea-salt SO4 plus NO3. This pattern of winter and summer regimes appears at other West Antarctic sites suggesting it may apply to the entire region. We show that a general picture of the patterns of variability in West Antarctica can best be drawn by using an analysis technique that fully exploits high resolution, multiparameter, multisite data sets

Long-Term Conditioning to Elevated pCO2 and Warming Influences the Fatty and Amino Acid Composition of the Diatom Cylindrotheca fusiformis

The unabated rise in anthropogenic CO2 emissions is predicted to strongly influence the ocean's environment, increasing the mean sea-surface temperature by 4°C and causing a pH decline of 0.3 units by the year 2100. These changes are likely to affect the nutritional value of marine food sources since temperature and CO2 can influence the fatty (FA) and amino acid (AA) composition of marine primary producers. Here, essential amino (EA) and polyunsaturated fatty (PUFA) acids are of particular importance due to their nutritional value to higher trophic levels. In order to determine the interactive effects of CO2 and temperature on the nutritional quality of a primary producer, we analyzed the relative PUFA and EA composition of the diatom Cylindrotheca fusiformis cultured under a factorial matrix of 2 temperatures (14 and 19°C) and 3 partial pressures of CO2 (180, 380, 750 μatm) for >250 generations. Our results show a decay of ∼3% and ∼6% in PUFA and EA content in algae kept at a pCO2 of 750 μatm (high) compared to the 380 μatm (intermediate) CO2 treatments at 14°C. Cultures kept at 19°C displayed a ∼3% lower PUFA content under high compared to intermediate pCO2, while EA did not show differences between treatments. Algae grown at a pCO2 of 180 μatm (low) had a lower PUFA and AA content in relation to those at intermediate and high CO2 levels at 14°C, but there were no differences in EA at 19°C for any CO2 treatment. This study is the first to report adverse effects of warming and acidification on the EA of a primary producer, and corroborates previous observations of negative effects of these stressors on PUFA. Considering that only ∼20% of essential biomolecules such as PUFA (and possibly EA) are incorporated into new biomass at the next trophic level, thepotential impacts of adverse effects of ocean warming and acidification at the base of the food web may be amplified towards higher trophic levels, which rely on them as source of essential biomolecules

A922 Sequential measurement of 1 hour creatinine clearance (1-CRCL) in critically ill patients at risk of acute kidney injury (AKI)

Meeting abstrac

Terrane history of the Iapetus Ocean as preserved in the northern Appalachians and western Caledonides

The Iapetus Ocean was the first ancient ocean to be identified following the development of plate tectonics; its history has been fundamental in relating orogenesis and plate motion. The ocean probably formed following 3-way rifting between Laurentia, Baltica, and Amazonia – West Africa (a block that became incorporated in Gondwana). Closure of the ocean trapped numerous terranes during the development of the Appalachian–Caledonide Orogen. Subsequent deformation, including late Paleozoic strike slip, transpression, and transtension, and Mesozoic stretching during Pangea breakup, must be taken into account in models for orogen development. Traditional analyses of Iapetan terranes have focussed on Cambrian sedimentary successions, and on isotopic criteria, to classify terranes into larger domains: Ganderia, Avalonia and Megumia. Detrital zircon data show that these domains did not cross the Iapetus as single entities, while paleomagnetic data reveal significant vertical-axis terrane rotations. We here review and interpret 17 paleomagnetic poles and >350 published detrital zircon data sets from the northern Appalachians and western Caledonides, using consistent and rigorous criteria for the selection and presentation of data. We place these data on an integrated stratigraphic chart to show timing relations and to seek constraints on the provenance and travel of terranes in the Iapetus Ocean. We distinguish groups of terranes that likely travelled together as terrane assemblages. In the Taconian/Grampian Orogeny, Furongian to Katian continent–arc collision involved off-margin blocks along the hyperextended Laurentian margin. In New England, early Taconian collision by 475 Ma involved the Gondwana-derived Moretown assemblage. An assemblage of the Bronson and Popelogan arc terranes probably arrived at the main Laurentian margin 25-30 Myr later. Subduction polarity reversal then led to the progressive accretion of additional terrane assemblages (Salinian Orogeny). The Miramichi–Victoria assemblage arrived close to the Ordovician–Silurian boundary. The Miramichi terrane underwent partial subduction in the Québec re-entrant, whereas the Victoria terrane was juxtaposed with the Newfoundland promontory without major metamorphism. In mid-Silurian time, an assemblage including the Gander terrane of Newfoundland and related portions of Britain and Ireland was accreted to Laurentia, along with Baltica (Scandian Orogeny). The St. Croix – La Poile assemblage may have been accreted slightly later, but is distinguished by the development of a Silurian arc–backarc system (coastal igneous belt) above a northwest-dipping subduction zone. The Avalon–Brookville assemblage encountered this system in Přídolí to Middle Devonian time (Acadian Orogeny), leading to the collapse of the backarc basin and northwest-vergent thrust emplacement onto Laurentia during sinistral transpression in the Appalachian Orogen. Acadian deformation involved mainly sinistral strike slip in Britain and Ireland. Several of the terranes that were accreted to the Laurentian margin carried internal records of earlier deformation that took place near Amazonia – West Africa in Early Ordovician time and earlier (Monian/Penobscottian Orogeny). The Iapetus Ocean thus contained a complex array of terranes, small ocean basins, arcs, and previously emplaced ophiolites analogous to modern southeast Asia. It closed to form a complex array of sutures in an orogen within which no single Iapetus suture can be clearly identified