Development of a MALDI MS-based platform for early detection of acute kidney injury

Purpose: Septic acute kidney injury (AKI) is associated with poor outcome. This can partly be attributed to delayed diagnosis and incomplete understanding of the underlying pathophysiology. Our aim was to develop an early predictive test for AKI based on the analysis of urinary peptide biomarkers by MALDI-MS. Experimental design: Urine samples from 95 patients with sepsis were analyzed by MALDI-MS. Marker search and multimarker model establishment were performed using the peptide profiles from 17 patients with existing or within the next 5 days developing AKI and 17 with no change in renal function. Replicates of urine sample pools from the AKI and non-AKI patient groups and normal controls were also included to select the analytically most robust AKI markers. Results: Thirty-nine urinary peptides were selected by cross-validated variable selection to generate a support vector machine multidimensional AKI classifier. Prognostic performance of the AKI classifier on an independent validation set including the remaining 61 patients of the study population (17 controls and 44 cases) was good with an area under the receiver operating characteristics curve of 0.82 and a sensitivity and specificity of 86% and 76%, respectively. Conclusion and clinical relevance: A urinary peptide marker model detects onset of AKI with acceptable accuracy in septic patients. Such a platform can eventually be transferred to the clinic as fast MALDI-MS test format

A cytological study of stimulation of the liver

In correlation with the ... [Introduction] the work for this paper was undertaken to demonstrate that the changes taking place in the secretory cells of the liver are identical with those in the nerve cells under both normal and abnormal stimuli and to prove that cloudy swelling is nothing else than a product of stimulation.--Introduction

KAJIAN PEMANFAATAN LUBANG BEKAS TAMBANG (VOID) SEBAGAI BENTUK RESOLUSI KONFLIK RONA AKHIR TAMBANG (Studi pada Void Eks Pit 7 PT. Sarolangun Prima Coal)

Penelitian ini bertujuan untuk mengetahui potensi pemanfaatan yang dapat dilakukan pada void eks pit 7 PT. SPC, dan untuk mengetahui bagaimana persepsi masyarakat terhadap rencana pemanfaatan void sebagai bentuk resolusi konflik keberadaan void. Berdasarkan hasil uji kualitas air void (parameter fisika dan kimia), nilai rata-rata pH perairan studi berkisar antara 5,56–6,35; Suhu 26,8-31,60C; TSS 28–62 mg/l; DO 3,35–4,38 mg/l; COD 23,41–36,65 mg/l; Amonia 0,086-0,265 mg/l; Nitrat 0,117-0,564 mg/l; Phospat 0,065-0,134 mg/l; logam Fe 0,08-0,154 mg/l, dan logam Pb 0,076-0,112 mg/l. Dilihat dari parameter biologi, ditemukan 29 jenis fitoplankton dari 5 kelas yaitu Bacillarophyceae (15 jenis), Chlorophyceae (3 jenis), Chrysophyceae (1 jenis), Cyanophyceae (9 Jenis) dan Dinophyceae (1 jenis). Terdapat 5 jenis fitoplankton yang predominan yaitu Nitzschia obtusa, Pinnularia viridis, Dinobrion sertularia, Oscillatoria granulate dan Oscillatoria Limnetica. Indeks Diversitas rata-rata perairan studi adalah 2,36, sedangkan Indeks Keseragaman (IE) rata-rata yang diperoleh adalah 0,70. Hal ini menunjukkan bahwa stabilitas komunitas biota perairan studi berada pada tingkatan sedang/kondisi perairan tercemar ringan. Mengacu pada PP 82 Tahun 2001, kualitas air void eks Pit 7 PT. SPC termasuk dalam kategori mutu air kelas IV, berpotensi untuk dimanfaatkan sebagai reservoar air (embung) untuk pengairan tanaman dan wisata pemandangan. Selanjutnya hasil wawancara menunjukkan bahwa masyarakat memperlihatkan persepsi positif terhadap rencana pemanfaatan void untuk tujuan tertentu sebagai bentuk resolusi konflik. Jika void tidak ditimbun kembali, pemanfaatan void sebagai embung merupakan bentuk pemanfaatan yang paling berpotensi menjadi bentuk resolusi konflik. Kata kunci : kualitas air, lubang tambang, pemanfatan void, resolusi konflik

The Medium Is the Danger: Discourse about Television among Amish and Ultra-Orthodox (Haredi) Women

This study shows how Old Order Amish and ultra-Orthodox women’s discourse about television can help develop a better understanding of the creation, construction, and strengthening of limits and boundaries separating enclave cultures from the world. Based on questionnaires containing both closed- and open-ended questions completed by 82 participants, approximately half from each community, I argue that both communities can be understood as interpretive communities that negatively interpret not only television content, like other religious communities, but also the medium itself. Their various negative interpretive strategies is discussed and the article shows how they are part of an “us-versus-them” attitude created to mark the boundaries and walls that enclave cultures build around themselves. The comparison between the two communities found only a few small differences but one marked similarity: The communities perceive avoidance of a tool for communication, in this case television, as part of the communities’ sharing, participation, and common culture

CREDs, CRUDs, and Catholic Scandals Experimentally examining the effects of religious paragon behaviour on co-religionist belief

Previous research on ‘Credibility Enhancing Displays’ (CREDs) suggests that long-term exposure to religious role models ‘practicing what they preach’ aids the acceptance of religious representations by cultural learners. Likewise, a considerable amount of anecdotal evidence implicates its opposite, perceived ‘religious hypocrisy’ (forthwith ‘Credibility Undermining Displays’ or ‘CRUDs’), as a factor in the rejection of religion. However, there is currently little causal evidence on whether behaviours of either kind displayed by religious authorities directly affect pre-existing religious belief. The current study investigated this question by priming Irish self-identified ‘Catholic Christian’ participants with either a clerical ‘CRED’ or ‘CRUD’ and subsequently measuring levels of explicit and implicit belief. Our results revealed no effects of immediate CRED or CRUD exposure on either implicit religious belief or three different measures of explicit religiosity. Instead, explicit (but not implicit) religiosity was predicted by past CRED exposure. Prospects and limitations of experimental approaches to CREDs/CRUDs are discussed

Mothers construct fathers: Destabilized patriarchy in La Leche League

This paper examines changing masculine ideals from the point of view of women homemakers through a case study of La Leche League, a maternalist organization dedicated to breastfeeding and mother primacy. We suggest two reasons for studying the League: first, an emerging literature suggests that changing norms are seeping into many such seemingly conservative groups, and second, the League continues to be highly successful among white, middle-class, married women. The paper looks at two aspects of masculinity, examining changes in the League through fieldwork, interviews, and content analysis, and finds that new norms of increased father involvement and decreased rights over women's bodies have both influenced League philosophy. We conclude that while in some respects a measure of the decline of men's patriarchal privileges, the League's changes also may contribute to a “restabilization” of male dominance in a modified, partial form.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/43548/1/11133_2004_Article_BF00990071.pd