Glutathionylation of Pea Chloroplast 2-Cys Prx and Mitochondrial Prx IIF Affects Their Structure and Peroxidase Activity and Sulfiredoxin Deglutathionylates Only the 2-Cys Prx

Together with thioredoxins (Trxs), plant peroxiredoxins (Prxs), and sulfiredoxins (Srxs) are involved in antioxidant defense and redox signaling, while their regulation by post-translational modifications (PTMs) is increasingly regarded as a key component for the transduction of the bioactivity of reactive oxygen and nitrogen species. Among these PTMs, S-glutathionylation is considered a protective mechanism against overoxidation, it also modulates protein activity and allows signaling. This study explores the glutathionylation of recombinant chloroplastic 2-Cys Prx and mitochondrial Prx IIF from Pisum sativum. Glutathionylation of the decameric form of 2-Cys Prx produced a change in the elution volume after FPLC chromatography and converted it to its dimeric glutathionylated form, while Prx IIF in its reduced dimeric form was glutathionylated without changing its oligomeric state. Mass spectrometry demonstrated that oxidized glutathione (GSSG) can glutathionylate resolving cysteine (Cys174), but not the peroxidatic equivalent (Cys52), in 2-Cys Prx. In contrast, GSSG was able to glutathionylate both peroxidatic (Cys59) and resolving (Cys84) cysteine in Prx IIF. Glutathionylation was seen to be dependent on the GSH/GSSG ratio, although the exact effect on the 2-Cys Prx and Prx IIF proteins differed. However, the glutathionylation provoked a similar decrease in the peroxidase activity of both peroxiredoxins. Despite growing evidence of the importance of post-translational modifications, little is known about the enzymatic systems that specifically regulate the reversal of this modification. In the present work, sulfiredoxin from P. sativum was seen to be able to deglutathionylate pea 2-Cys Prx but not pea Prx IIF. Redox changes during plant development and the response to stress influence glutathionylation/deglutathionylation processes, which may represent an important event through the modulation of peroxiredoxin and sulfiredoxin proteins.This research was supported by MICINN, Spain (BFU2014-52452-P co-financed by FEDER) and Seneca Foundation, Murcia, Spain (Excellence Group 19876/GERM/15).Peer reviewedPeer Reviewe

The Recipe : Making Latin-American Art in Canada

"At OBORO, The Recipe offers to see and hear revised/expanded/recomposed or site-specific versions of the works first presented at Sur Gallery (Toronto, 2018), revealing the wide spectrum of influences and references that shape these artists’ imaginaries, beyond their countries of origin or cultural roots." -- Publisher's website

¿Existen estereotipos genéricos en la escuela?

Una de las conquistas del siglo XX fue el acceso a la educación por parte de las mujeres. Se ha logrado así una igualdad formal, que no necesariamente alude a la igualdad real, pues la práctica es mucho más rica y nos muestra la presencia de un currículo oculto en la escuela que es simultáneo al currículo formal y que perpetúa estereotipos y prejuicios genéricos, sobre la base de una sociedad patriarcal y androcéntrica. El presente trabajo, pretende abordar la escuela desde una perspectiva de género. ABSTRACT IS THERE A GENDER'S STEREOTYPES AT SCHOOL? The access to the superior education for women was an achievement of the XX century. School educates women and men in the same way. This is apparently, because there is an informal curriculum which reproduces genre's stereotypes. We still have a patriarchal and andocentric society, that 's why we aspirate to analyze the school since a gender's perspective

Projeto de intervenção para conscientização dos pacientes da Unidade de Saúde sobre o consumo inadequado de Bensodiazepínicos

Introdução: O presente projeto de intervenção tem como foco o uso inadequado de benzodiazepínicos em usuários da unidade básica de saúde UBS, Jardim Itaú, Municipio Itaperucu, estado Paraná. Os Benzodiazepínicos são medicamentos muito eficazes, que reduzem ansiedade, indicados em casos de insônia e outros. O tempo de uso deste medicamentos não deve ser prolongado, a fim de evitar quadros de dependências e outras complicações. Para isso as ações da UBS de caráter educativo e preventivo, com equipe multiprofissional devem ser priorizadas. Objetivo: O objetivo do trabalho foi propor um plano de intervenção para combater o uso indevido e indiscriminado de benzodiazepínicos de usuários na unidade básica de saúde. Metodologia: Primeiramente realizou se o diagnostico situacional de saúde na unidade, através dele foram levantados dados através de fichas dos usuários e o problema encontrado e priorizado foi o grande número de pacientes em uso inadequado de benzodiazepínicos. Serão realizadas reuniões semanais, na Unidade de saúde ou no Centro , com o grupo Ouro e os profissionais da saúde, nas quais cada dia será discutido um tema relacionado à medicação e doenças envolvidas, sob formas variadas, de acordo com o profissional selecionado para a data. Serão realizadas reuniões semanais, na Unidade de saúde ou no Centro , com o grupo Ouro e os profissionais da saúde, nas quais cada dia será discutido um tema relacionado à medicação e doenças envolvidas, sob formas variadas, de acordo com o profissional selecionado para a data. Resultados esperados: Através de atitude ativa, persistente e duradoura de toda a equipe, os pacientes do “Jardim Itaú alcançarão a percepção dos riscos que a medicação traz a eles e consequentemente o combate ao uso inadvertido, inadequado e excessivo dessa. Os professionais da saúde devem ter suas ações pautadas conscientização dos usuários sobre os benzodiazepínicos, de forma inserir o usuário em seus cuidados continuamente

Embarazo en la adolescencia: prevalenciaencia, riesgos y complicaciones

Se realizó un estudio de casos y controles para analizar la incidencia, así como los riesgos y complicaciones del embarazo y el parto en la adolescencia, en el hospital municipal de Santa Cruz del Sur, desde el lro. de enero de hasta el 31 de diciembre de 1999 Se tomó como grupo estudio a las 132 adolescentes que parieron en esta etapa y se comparó con un grupo control de 132 gestantes de 20 años o más que parieron en igual fecha. Esta información fue recogida de los expedientes clínicos de las pacientes y del libro de parto y llevada a encuestas. El bajo peso materno estuvo presente en el 59, 8 % de las adolescentes; el 87, 1 % de estas eran amas de casa; el 78 % presentó alguna enfermedad durante el embarazo; el 78 % de estas gestantes y sus productos sufrieron mayor número de complicaciones, además de asociarse con mayor frecuencia las complicaciones posparto para la madre. Este estudio nos muestra todos los riesgos que implica un embarazo en estas edades, por lo que insistimos en la importancia de disminuirlos al mínimo

Infections of the central nervous system in pediatric patients clinical epidemiological aspects

Introduction: infections of the central nervous system constitute a health problem on the planet. Objective: to clinically epidemiologically characterize   children with central nervous system infections admitted to be Pepe Portilla Provincial Teaching Pediatric Hospital of Pinar del Río in the years 2020­2021. Method: observational, descriptive, cross-sectional study. Universe 63 patients with diagnosis of central nervous system infection. A sample of 40 patients was selected through simple random sampling .Descriptive and inferential statistics were used. Results: meningoencephalitis of viral etiology predominated in 82,5 % of the sample, with ages from 11 to 13 years in 75 %. The male sex represented 67, 5 percent of the cases studied. Fever was identified in 97,5 % of patients as the most frequent symptom. Non­breastfeeding was found in 92,5 % of the patients as a risk factor associated with central nervous system infections. In 2020, epilepsy was reported in 12,5 % of patients as the most frequent complication and in 2021 it represented 20 %. The lethality of the infection was shown in 5 % of the cases in 2020 and in 2021 the fatal outcome occurred in 2,5 %. Conclusions: the important social impact of the central nervous system infections in pediatric patients was evidenced by their clinical evolution, fatal resolution or the presence of sequelae

Comportamiento de diferentes explantes de arroz (Variedad IACuba-28) en la transformación genética mediante Agrobacterium tumefaciens

The present work describes the behavior of four rice explants: coleoptiles, embryogenic calli, earliest calli and mature embryos, in genetic transformation via Agrobacterium tumefaciens, applying the protocol established for cuban rice cultivar in our laboratory. We used GUS activity as a signal for measuring transformation efficiency. The best results were obtained with embryogenic calli, in which transformation efficiency was 37 %, and an average of 11.20 blue spots per transformed calli was obtained. In coleoptiles and earliest calli was detected ß-Glucuronidase expression, but it was not detected in mature embryos, whose tissues were affected seriously by bacterial infection. We demonstrated the versatility of this transformation procedure in different tissues of IACuba-28 rice cultivar.Key words: ß-Glucuronidase, embryogenic calli, transient activityEn este trabajo se describe el comportamiento de cuatro tipos de explantes de arroz: coleoptilos, callos embriogénicos, callos primarios y embriones maduros, en la transformación genética vía Agrobacterium tumefaciens. Como parámetro para evaluar la eficiencia de transformación se utilizó la actividad GUS. Los mejores resultados se obtuvieron con los callos embriogénicos, con un 37% de eficiencia de transformación y un promedio de 11.20 puntos azules por callo transformado. En los callos primarios y los coleoptilos también fue detectada la expresión de la ß-Glucuronidasa, pero no fue así en los embriones, donde la infección bacteriana causó daños tisulares importantes. Se demuestra la versatilidad del procedimiento de transformación aplicado en diferentes tejidos de la variedad de arroz IACuba-28.Palabras clave: actividad transitoria, ß-Glucuronidasa, callos embriogénico

Características neuropsicológicas del pensamiento rumiativo en pacientes sometidos a cirugía cardíaca con y sin circulación extracorpórea

This paper presents the results of the research “Comparison of neuropsychological performance of thought on patients subjected to heart surgery with and without extracorporeal circulation”, carried out at the Cardiocentro Ernesto Che Guevara (Ernesto Che Guevara Heart Clinic), Santa Clara, Villa Clara, Cuba, from October 2010 to april 2011. The sample was composed of 40 patients that underwent heart surgery, divided in two groups according to whether they had extracorporeal circulation during the surgery. For the development of the research we employed psychological analysis of the elements from clinical records, a semistructured psichological interview of the patient and the While-Bear Suppression Inventory (wbsi). The analysis of acquired data confirmed the presence of greater manifestations of ruminative and suppressive thought in patients that underwent surgery with extracorporeal circulation; we highlight the presence of intrusive and unwanted thoughts and self-distraction.El artículo presenta los resultados de la investigación “Comparación del funcionamiento neuropsicológico del pensamiento en pacientes sometidos a cirugía cardíaca con y sin circulación extracorpórea”, llevada a cabo en el Cardiocentro Ernesto Che Guevara, Santa Clara, Villa Clara, Cuba, de octubre del 2010 a abril del 2011. La muestra estuvo conformada por 40 pacientes sometidos a cirugía cardíaca, divididos en dos grupos: uno con circulación extracorpórea y otro sin ella. Para el desarrollo de la investigación se utilizaron técnicas como el análisis psicológico de los elementos contenidos en la historia clínica, una entrevista psicológica semiestructurada al paciente y el While-Bear Supresión Inventory (wbsi). El análisis de los datos obtenidos permitió comprobar la presencia de manifestaciones más elevadas de pensamiento rumiativo y supresivo en los pacientes sometidos a cirugía con circulación extracorpórea; se resalta la presencia de pensamientos intrusivos no deseados y la autodistracción

Phenotypic and Genetic Characterization of Antimicrobial Profiles of Helicobacter pylori Strains in Cuba

The study evaluated the antibiotic resistance patterns of Helicobacter pylori strains against metronidazole and clarithromycin in a hospital in Havana, Cuba. Eighty-five percent, 22.5%, and 10% of 40 H. pylori strains investigated were resistant to metronidazole, ciprofloxacin, and clarithromycin respectively but all were susceptible to amoxicillin and tetracycline. RdxA truncation was found only in metronidazole-resistant strains. In such strains, reported are eight and two novel mutations in the rdxA and frxA genes respectively. Two-point mutations in the 23S rRNA genes of clarithromycin-resistant strains were detected. A high prevalence of metronidazole resistance was found in Cuban H. pylori strains. Mutations in the rdxA gene may contribute more significantly than frxA gene to the high level of resistance to metronidazole. This study supports the need to continue monitoring the antibiotic susceptibility in H. pylori in Cuba to guide the treatment of such infection